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The map label for this gene is thcR [H]

Identifier: 116249356

GI number: 116249356

Start: 749326

End: 750378

Strand: Direct

Name: thcR [H]

Synonym: pRL120693

Alternate gene names: 116249356

Gene position: 749326-750378 (Clockwise)

Preceding gene: 116249354

Following gene: 116249359

Centisome position: 86.13

GC content: 57.45

Gene sequence:

>1053_bases
GTGGAAGAGGAAAGAACCGAGTTGCAGATATCGTTGAGATCCGAAAATACGGCGCGCGCTCCCGAGCGTGACGCATACGG
GTTTGCGCCAACGCATGAAGGCAGTGACTTCGAGTCGATGGTTCGTGCCTATTCTGCAGGCTACGGGGTGTTTGGCGCGA
AGCCGCTGAGCAACGACCGGGCCTTTGCCTGGGCAGCGGACTTGAGAACAAGTGAGGCATTTACGGCACTTCATTCCGTC
TATCAGAGCTCCTGGACAATACGAACGCTCGATGAGACGCCGCAACACCTTGCGTTTTACATTCCGCACACGGGATCCTT
CCGGCTGTCCATTGGAAGAAAGGTGGTTGAAAGTGGAGCGGGTCATCTTCTCATGGCCAACAACCACGAAGCTGGCGATC
GTTTCATCCAAGGCCCGCACTGCTCGGATGCTCTCTTCCTGGACTGGAAAATTGTCAGGCGAATGCTCGTTTCACTGGTG
GAAACGCCGATCCTCGAGTCGCTTGACCTTGAGCCCGTTGTGGATCTCGCAACACCATCGGGTCAGCTTATCGGCAGTCT
GGTGCAGACGATCGTGCAGGGCATGCGCAATGGCGGTCCGCTTCTGTCTTCACCTCTGGCCTTGGCGGCGATGAGTGAAA
CGCTCGCTAACCTTGTGATTCGGTTTGGCCGCCACCGTCTTTCCGGCCATTTGGAAAAACAGAAAGTGTGTTTGGTCGCG
CCGTGGCATGTCCGGCGTGCTATCGACTATATGCACGCCAATATCGCAGAGCCCCTCACCATGACGATGGTTGCCGATGG
TGTCGGTGTTTCACTTCGCGCGCTGCAAACCGGTTTCAGGGCCTTCCGGGGAACTTCACCGGCCGGTTACTTGCGCACGA
TCAGGCTGCAGGCAGCCCGCGACCAGTTGCAGGATCCGATGAATCAGCGATCCGTCCGCGAAATCTGCGCGATGTGGGGT
TTTTCTCATGCCGGCAGGTTCTCTATTATCTACCGCAGCGCCTTTGGAGAAAGCCCGCGCGATACGCGCCTGCGGGCTGA
GCGCTTGCGATAA

Upstream 100 bases:

>100_bases
ACGAAGGTGGAGGTGATCGTGCCCCATGCGTTTGGAAGGCACGATATCGGCGACGCAGCGATCTCAAGCGAGATGGAGCG
CTTGATAGGCGGGTCGGGGT

Downstream 100 bases:

>100_bases
AGAAGGACGCCGAAAAGTGTGAACGGTTTTCGGACAACATCATGCTCTAGAGCCTTTCCGGGTTAGATTGAAGCATTCTG
TGGGCTCAAACGGAGTCGGA

Product: AraC family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MEEERTELQISLRSENTARAPERDAYGFAPTHEGSDFESMVRAYSAGYGVFGAKPLSNDRAFAWAADLRTSEAFTALHSV
YQSSWTIRTLDETPQHLAFYIPHTGSFRLSIGRKVVESGAGHLLMANNHEAGDRFIQGPHCSDALFLDWKIVRRMLVSLV
ETPILESLDLEPVVDLATPSGQLIGSLVQTIVQGMRNGGPLLSSPLALAAMSETLANLVIRFGRHRLSGHLEKQKVCLVA
PWHVRRAIDYMHANIAEPLTMTMVADGVGVSLRALQTGFRAFRGTSPAGYLRTIRLQAARDQLQDPMNQRSVREICAMWG
FSHAGRFSIIYRSAFGESPRDTRLRAERLR

Sequences:

>Translated_350_residues
MEEERTELQISLRSENTARAPERDAYGFAPTHEGSDFESMVRAYSAGYGVFGAKPLSNDRAFAWAADLRTSEAFTALHSV
YQSSWTIRTLDETPQHLAFYIPHTGSFRLSIGRKVVESGAGHLLMANNHEAGDRFIQGPHCSDALFLDWKIVRRMLVSLV
ETPILESLDLEPVVDLATPSGQLIGSLVQTIVQGMRNGGPLLSSPLALAAMSETLANLVIRFGRHRLSGHLEKQKVCLVA
PWHVRRAIDYMHANIAEPLTMTMVADGVGVSLRALQTGFRAFRGTSPAGYLRTIRLQAARDQLQDPMNQRSVREICAMWG
FSHAGRFSIIYRSAFGESPRDTRLRAERLR
>Mature_350_residues
MEEERTELQISLRSENTARAPERDAYGFAPTHEGSDFESMVRAYSAGYGVFGAKPLSNDRAFAWAADLRTSEAFTALHSV
YQSSWTIRTLDETPQHLAFYIPHTGSFRLSIGRKVVESGAGHLLMANNHEAGDRFIQGPHCSDALFLDWKIVRRMLVSLV
ETPILESLDLEPVVDLATPSGQLIGSLVQTIVQGMRNGGPLLSSPLALAAMSETLANLVIRFGRHRLSGHLEKQKVCLVA
PWHVRRAIDYMHANIAEPLTMTMVADGVGVSLRALQTGFRAFRGTSPAGYLRTIRLQAARDQLQDPMNQRSVREICAMWG
FSHAGRFSIIYRSAFGESPRDTRLRAERLR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=95, Percent_Identity=37.8947368421053, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38922; Mature: 38922

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEERTELQISLRSENTARAPERDAYGFAPTHEGSDFESMVRAYSAGYGVFGAKPLSNDR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
AFAWAADLRTSEAFTALHSVYQSSWTIRTLDETPQHLAFYIPHTGSFRLSIGRKVVESGA
CEEEEHHCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCEEEHHHHHHHHCCC
GHLLMANNHEAGDRFIQGPHCSDALFLDWKIVRRMLVSLVETPILESLDLEPVVDLATPS
CEEEEECCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
GQLIGSLVQTIVQGMRNGGPLLSSPLALAAMSETLANLVIRFGRHRLSGHLEKQKVCLVA
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PWHVRRAIDYMHANIAEPLTMTMVADGVGVSLRALQTGFRAFRGTSPAGYLRTIRLQAAR
CHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DQLQDPMNQRSVREICAMWGFSHAGRFSIIYRSAFGESPRDTRLRAERLR
HHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MEEERTELQISLRSENTARAPERDAYGFAPTHEGSDFESMVRAYSAGYGVFGAKPLSNDR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
AFAWAADLRTSEAFTALHSVYQSSWTIRTLDETPQHLAFYIPHTGSFRLSIGRKVVESGA
CEEEEHHCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCEEEHHHHHHHHCCC
GHLLMANNHEAGDRFIQGPHCSDALFLDWKIVRRMLVSLVETPILESLDLEPVVDLATPS
CEEEEECCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
GQLIGSLVQTIVQGMRNGGPLLSSPLALAAMSETLANLVIRFGRHRLSGHLEKQKVCLVA
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PWHVRRAIDYMHANIAEPLTMTMVADGVGVSLRALQTGFRAFRGTSPAGYLRTIRLQAAR
CHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DQLQDPMNQRSVREICAMWGFSHAGRFSIIYRSAFGESPRDTRLRAERLR
HHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]