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The map label for this gene is dgoD [H]

Identifier: 116249251

GI number: 116249251

Start: 636948

End: 638180

Strand: Direct

Name: dgoD [H]

Synonym: pRL120586

Alternate gene names: 116249251

Gene position: 636948-638180 (Clockwise)

Preceding gene: 116249250

Following gene: 116249252

Centisome position: 73.21

GC content: 63.91

Gene sequence:

>1233_bases
GTGACGAACGGAAAGACATATGCCCTGCGGCTCGCGGGCCGTGAGGGGCAGGGGATCCGAGACATGCGCATCAAGACCAT
AGAGGCCTGGTGGATTAAAATTCCCATCGAGGCGAACCGTCAGCACCGCAGCGACTTCGGCCGACTGACGACCTTCGATA
GTGCGATCCTGCGGATCGAAACGGACGACGGCATCGTCGGCTGGGGAGAGGGCAAGAATGCCGCGGGCAGTGCGGGCACT
TATGGCACGCTGGTACACATGCTCAATCACGAGATTGGGCCGAAGCTCATCGATCGCGATCCCGCCGACATCTCCACAAT
ATGGGAGATGCTCTATAACGGCGTACGCCACGAGACGGCGGCGAGTTCGGGTCACGCCATGCCCGAGATCGCGCGGCGCG
GCCTGTCCGTCGCGGCGATCAGCGCGGTCGACATAGCGCTCTGGGACATTCTCGGCAAGTCGCTCGGCGTCCCCGTCTGG
AAGCTGCTGGGTGGGCGCAAGGCGGACAGGTTGCCCGCCTATGCCTCGGGCGGTTGGGAGAGCGTGGACCGGATCGGCGA
ACAGCTCCGGTCCTATGTCGCGGCCGGTGGTTTCAAGTCGGTCAAGATGCGCGTCGGCGCGATGGATCGTGCGCTGCATG
TCTCGGCATCGCGCGTGCGGGCGGCTCGCAAGGCAATCGGCCCCGACGTCGACCTGATGGTCGATGCGCACGGCACCTAT
ACTGTTGCCGAGGCAAAGCGCTTCATCCAGATGGTCGCCGATTGCGACCTCGCCTGGTTCGAGGAGCCGGTGATAGCGGA
TGACAAGCCGGGCATGGCGGAGGTGCGCGCGGCTGGGAACGTGCCGATCGCTGCGGGCGAGAGCGAGGCGACGCGTTTTG
CCTTCCGAGATCTTGCCATGCTTCGGGCCGCCGACATATTCCAGCCGGATCCCGCCTTCTGCGGCGGCATTACGGAGGCG
ATGCGCATCAGTTCGCTCGCCAGCGCCTTCAACCTCCGTTTCGCGCCGCATCTTTGGGCCGGCGCGCCCTGCTTCTTCTC
CGGCCTGCACTTGTGCGCCGCTTCCCCGGCAAGCTTCATCATCGAATATTCGCTCGGCGCCAATCCGATGATCCACGATC
TTGTCGAGGACACTGTGTCGGTGAAAGACGGTATGGTAGAGATCCCGGACAGGCCTGGCCTCGGCTTCACGATCAATCAG
CGGGTCCTGGAGACTCACGCGCAAAGACAGTGA

Upstream 100 bases:

>100_bases
AGCTGAAATTTATCCAAGACCACGGCTCGAGCCTTCTCACTGAGAAGTTCTACGACGATCAGATGCGGATCACTGCCGAG
ACGATCCTGCCGCTGATCAA

Downstream 100 bases:

>100_bases
CGATGAAAGAAAATTCCCTCCTCTCCGATCTCGCGGCCCATCTTTTCTCGAACTCGAGCGGCAACGGCCGTACGCCTTCG
GAGCGAGAGCTTGCGGAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTNGKTYALRLAGREGQGIRDMRIKTIEAWWIKIPIEANRQHRSDFGRLTTFDSAILRIETDDGIVGWGEGKNAAGSAGT
YGTLVHMLNHEIGPKLIDRDPADISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVW
KLLGGRKADRLPAYASGGWESVDRIGEQLRSYVAAGGFKSVKMRVGAMDRALHVSASRVRAARKAIGPDVDLMVDAHGTY
TVAEAKRFIQMVADCDLAWFEEPVIADDKPGMAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEA
MRISSLASAFNLRFAPHLWAGAPCFFSGLHLCAASPASFIIEYSLGANPMIHDLVEDTVSVKDGMVEIPDRPGLGFTINQ
RVLETHAQRQ

Sequences:

>Translated_410_residues
MTNGKTYALRLAGREGQGIRDMRIKTIEAWWIKIPIEANRQHRSDFGRLTTFDSAILRIETDDGIVGWGEGKNAAGSAGT
YGTLVHMLNHEIGPKLIDRDPADISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVW
KLLGGRKADRLPAYASGGWESVDRIGEQLRSYVAAGGFKSVKMRVGAMDRALHVSASRVRAARKAIGPDVDLMVDAHGTY
TVAEAKRFIQMVADCDLAWFEEPVIADDKPGMAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEA
MRISSLASAFNLRFAPHLWAGAPCFFSGLHLCAASPASFIIEYSLGANPMIHDLVEDTVSVKDGMVEIPDRPGLGFTINQ
RVLETHAQRQ
>Mature_409_residues
TNGKTYALRLAGREGQGIRDMRIKTIEAWWIKIPIEANRQHRSDFGRLTTFDSAILRIETDDGIVGWGEGKNAAGSAGTY
GTLVHMLNHEIGPKLIDRDPADISTIWEMLYNGVRHETAASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVWK
LLGGRKADRLPAYASGGWESVDRIGEQLRSYVAAGGFKSVKMRVGAMDRALHVSASRVRAARKAIGPDVDLMVDAHGTYT
VAEAKRFIQMVADCDLAWFEEPVIADDKPGMAEVRAAGNVPIAAGESEATRFAFRDLAMLRAADIFQPDPAFCGGITEAM
RISSLASAFNLRFAPHLWAGAPCFFSGLHLCAASPASFIIEYSLGANPMIHDLVEDTVSVKDGMVEIPDRPGLGFTINQR
VLETHAQRQ

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=413, Percent_Identity=25.181598062954, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI186972148, Length=332, Percent_Identity=26.2048192771084, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI48994953, Length=365, Percent_Identity=31.5068493150685, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1787864, Length=383, Percent_Identity=25.8485639686684, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI226510960, Length=317, Percent_Identity=27.1293375394322, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44468; Mature: 44337

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNGKTYALRLAGREGQGIRDMRIKTIEAWWIKIPIEANRQHRSDFGRLTTFDSAILRIE
CCCCCEEEEEEECCCCCCCHHHHEEEEEEEEEEEEECCCCHHHHHHCCEEEECCEEEEEE
TDDGIVGWGEGKNAAGSAGTYGTLVHMLNHEIGPKLIDRDPADISTIWEMLYNGVRHETA
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHCHHHHHH
ASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWE
CCCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHH
SVDRIGEQLRSYVAAGGFKSVKMRVGAMDRALHVSASRVRAARKAIGPDVDLMVDAHGTY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCE
TVAEAKRFIQMVADCDLAWFEEPVIADDKPGMAEVRAAGNVPIAAGESEATRFAFRDLAM
EHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHH
LRAADIFQPDPAFCGGITEAMRISSLASAFNLRFAPHLWAGAPCFFSGLHLCAASPASFI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
IEYSLGANPMIHDLVEDTVSVKDGMVEIPDRPGLGFTINQRVLETHAQRQ
EEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure 
TNGKTYALRLAGREGQGIRDMRIKTIEAWWIKIPIEANRQHRSDFGRLTTFDSAILRIE
CCCCEEEEEEECCCCCCCHHHHEEEEEEEEEEEEECCCCHHHHHHCCEEEECCEEEEEE
TDDGIVGWGEGKNAAGSAGTYGTLVHMLNHEIGPKLIDRDPADISTIWEMLYNGVRHETA
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHCHHHHHH
ASSGHAMPEIARRGLSVAAISAVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWE
CCCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHH
SVDRIGEQLRSYVAAGGFKSVKMRVGAMDRALHVSASRVRAARKAIGPDVDLMVDAHGTY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCE
TVAEAKRFIQMVADCDLAWFEEPVIADDKPGMAEVRAAGNVPIAAGESEATRFAFRDLAM
EHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHH
LRAADIFQPDPAFCGGITEAMRISSLASAFNLRFAPHLWAGAPCFFSGLHLCAASPASFI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
IEYSLGANPMIHDLVEDTVSVKDGMVEIPDRPGLGFTINQRVLETHAQRQ
EEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]