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The map label for this gene is lutR [H]

Identifier: 116249252

GI number: 116249252

Start: 638177

End: 638875

Strand: Direct

Name: lutR [H]

Synonym: pRL120587

Alternate gene names: 116249252

Gene position: 638177-638875 (Clockwise)

Preceding gene: 116249251

Following gene: 116249253

Centisome position: 73.35

GC content: 62.23

Gene sequence:

>699_bases
GTGACGATGAAAGAAAATTCCCTCCTCTCCGATCTCGCGGCCCATCTTTTCTCGAACTCGAGCGGCAACGGCCGTACGCC
TTCGGAGCGAGAGCTTGCGGAGCATTTCGGCGTCAGTCGGGGCCAAATTCGTGAGGCACTGGCCATTCTGGAGGCCATGC
GCATCGTCGAGCGCCGGGCCAAGTCGGGCATCTATCTGACGACCACGGAGGCGAGCGTGGAGGCAATGGCGCTTTTTGCC
CGCGCCGGCGTGCCGCTTGATCCTATCCTGATCTACGAGACCGTGGAGCTTCGCAAGATCCATGAGATCAAGGCCGCGGA
GCTCGCCTGCGATCGGGCGACGGAGGAGAATTACCAGCACTTGCGCGATATCCTCGCGGCGTCGGAGGCGAAGCTTGCCG
CCGGCGAGGGGCTGGCGCGCGAGGACCGAGATTTTCATTTGGAGATCGTCCGGGCCACCAAGAACAGCGTCTTCCATCGG
GTGTGCAGCGTCTATTACGTCATGGGCGAGCACCGCCTGCCGATCTATTTTGCCGACGCCGCCCGCAGCCGGCGCTCGCA
TGAGGAACATATCCGCATCTACGAGGCGCTGCTTGCCCGAGACGGCAATCTGGCCCAGGCGCTGATGAGCGCACATCTTC
AAGGTGCGGAAAGCTATTGGAAGGGTCTCATCGGTGGTCCAGCGACGTCAGCCGGATAG

Upstream 100 bases:

>100_bases
ACACTGTGTCGGTGAAAGACGGTATGGTAGAGATCCCGGACAGGCCTGGCCTCGGCTTCACGATCAATCAGCGGGTCCTG
GAGACTCACGCGCAAAGACA

Downstream 100 bases:

>100_bases
GCGCAACCGGCGGGAGGGCCGATGAGCTTCATCTTTTCCACACATCCCCTGCACCCTGCGGCCAAGTCCATGCTTGAGGC
TGCGGGTGATCTGCGCGTCG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTMKENSLLSDLAAHLFSNSSGNGRTPSERELAEHFGVSRGQIREALAILEAMRIVERRAKSGIYLTTTEASVEAMALFA
RAGVPLDPILIYETVELRKIHEIKAAELACDRATEENYQHLRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFHR
VCSVYYVMGEHRLPIYFADAARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAESYWKGLIGGPATSAG

Sequences:

>Translated_232_residues
MTMKENSLLSDLAAHLFSNSSGNGRTPSERELAEHFGVSRGQIREALAILEAMRIVERRAKSGIYLTTTEASVEAMALFA
RAGVPLDPILIYETVELRKIHEIKAAELACDRATEENYQHLRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFHR
VCSVYYVMGEHRLPIYFADAARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAESYWKGLIGGPATSAG
>Mature_231_residues
TMKENSLLSDLAAHLFSNSSGNGRTPSERELAEHFGVSRGQIREALAILEAMRIVERRAKSGIYLTTTEASVEAMALFAR
AGVPLDPILIYETVELRKIHEIKAAELACDRATEENYQHLRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFHRV
CSVYYVMGEHRLPIYFADAARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAESYWKGLIGGPATSAG

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=201, Percent_Identity=29.8507462686567, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25705; Mature: 25573

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKENSLLSDLAAHLFSNSSGNGRTPSERELAEHFGVSRGQIREALAILEAMRIVERRA
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KSGIYLTTTEASVEAMALFARAGVPLDPILIYETVELRKIHEIKAAELACDRATEENYQH
HCCEEEEECCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFHRVCSVYYVMGEHRLPIYFADA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCH
ARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAESYWKGLIGGPATSAG
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TMKENSLLSDLAAHLFSNSSGNGRTPSERELAEHFGVSRGQIREALAILEAMRIVERRA
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KSGIYLTTTEASVEAMALFARAGVPLDPILIYETVELRKIHEIKAAELACDRATEENYQH
HCCEEEEECCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDILAASEAKLAAGEGLAREDRDFHLEIVRATKNSVFHRVCSVYYVMGEHRLPIYFADA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCH
ARSRRSHEEHIRIYEALLARDGNLAQALMSAHLQGAESYWKGLIGGPATSAG
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]