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The map label for this gene is adhI [H]

Identifier: 116249188

GI number: 116249188

Start: 569136

End: 570263

Strand: Direct

Name: adhI [H]

Synonym: pRL120524

Alternate gene names: 116249188

Gene position: 569136-570263 (Clockwise)

Preceding gene: 116249187

Following gene: 116249189

Centisome position: 65.42

GC content: 62.77

Gene sequence:

>1128_bases
ATGGACGTTCGCGCCGCCGTTGCCGTTCAGGCAGGAAAACCGCTCGAAGTGATGACCGTGCAGCTGGAAGGCCCGAAGGC
CGGCGAAGTGCTGGTGGAGGTCAAGGCGACGGGCATCTGCCACACCGATGATTTCACCTTGTCGGGCGCCGATCCGGAAG
GCCTCTTCCCGGCCATTCTCGGCCATGAAGGCGCCGGCATCGTCGTCGATGTCGGCCCCGGCGTCACCTCGGTGAAGAAG
GGCGACCACGTCATTCCGCTCTACACCCCGGAATGCCGCGAATGCTATTCCTGCACCTCGCGCAAGACCAATCTCTGCAC
CTCGATTCGATCAACCCAGGGCCAGGGCGTGATGCCGGACGGCACCTCGCGCTTCTCGATCGGCAAGGACAAGATCCACC
ACTATATGGGCTGCTCGACCTTTTCGAACTTCACCGTGCTGCCGGAGATCGCCCTTGCCAAGGTCAATCCCGACGCTCCG
TTCGACAAGATTTGCTACATCGGCTGCGGTGTGACCACTGGCATCGGCGCCGTCATCAACACCGCCAAGGTGGAGATCGG
CGCCACGGCGATCGTCTTCGGGCTTGGCGGTATCGGCCTCAACGTGCTTCAGGGCCTGCGTCTTGCCGGTGCCGACATGA
TCATCGGCGTCGACATCAACCCTGATCGCAAGGCCTGGGGCGAAAAGTTCGGCATGACCCACTTCGTCAATCCGAAGGAG
GTCGGCGACGACATCGTGCCCTATCTCGTCAACCTGACGAAGCGCCACGGCGATCTGATCGGCGGCGCCGACTACACCTT
CGACTGCACCGGCAACACCAAGGTGATGCGTCAGGCGCTGGAAGCATCGCACCGCGGCTGGGGCAAGTCGATTATCATCG
GCGTTGCCGGCGCCGGCCAGGAAATCTCCACCCGTCCGTTCCAGCTGGTCACCGGCCGCAACTGGATGGGCACCGCCTTC
GGCGGCGCGCGCGGCCGCACCGACGTGCCGAAGATCGTCGACTGGTACATGCAGGGCAAGATCCAGATCGATCCGATGAT
CACCCACACAATGCCGCTCGAAGACATCAACAAGGGCTTCGAGCTGATGCACAAGGGCGAAAGCATCCGCGGCGTGGTCG
TGTACTGA

Upstream 100 bases:

>100_bases
GAGCATGAAATCTCGGCTGTGACGCAGCAGCAGATCGGTGAGAAGCCGGCCCCTTCGAATATGTAGCATCAGAATTCAAT
TTAAGAGGAAGGATATCAAA

Downstream 100 bases:

>100_bases
GCCATGAAGACGATCTCGACCGAAAATTCTCATGGCGGCACCCAGGGCGTTTACCTCGATCGCTCGGACGCCTGCGACTG
CGACATGACCTTTGCGGTCT

Product: putative alcohol dehydrogenase class III

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDVRAAVAVQAGKPLEVMTVQLEGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCTSRKTNLCTSIRSTQGQGVMPDGTSRFSIGKDKIHHYMGCSTFSNFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEIGATAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
VGDDIVPYLVNLTKRHGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINKGFELMHKGESIRGVVVY

Sequences:

>Translated_375_residues
MDVRAAVAVQAGKPLEVMTVQLEGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCTSRKTNLCTSIRSTQGQGVMPDGTSRFSIGKDKIHHYMGCSTFSNFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEIGATAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
VGDDIVPYLVNLTKRHGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINKGFELMHKGESIRGVVVY
>Mature_375_residues
MDVRAAVAVQAGKPLEVMTVQLEGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCTSRKTNLCTSIRSTQGQGVMPDGTSRFSIGKDKIHHYMGCSTFSNFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEIGATAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
VGDDIVPYLVNLTKRHGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINKGFELMHKGESIRGVVVY

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=56.3002680965147, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI71565152, Length=380, Percent_Identity=42.8947368421053, Blast_Score=325, Evalue=3e-89,
Organism=Homo sapiens, GI34577061, Length=375, Percent_Identity=43.2, Blast_Score=321, Evalue=6e-88,
Organism=Homo sapiens, GI4501929, Length=375, Percent_Identity=42.9333333333333, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI262073058, Length=376, Percent_Identity=42.0212765957447, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI71743840, Length=376, Percent_Identity=42.0212765957447, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI4501933, Length=375, Percent_Identity=42.1333333333333, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI156523966, Length=371, Percent_Identity=40.7008086253369, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI4501939, Length=364, Percent_Identity=40.9340659340659, Blast_Score=295, Evalue=3e-80,
Organism=Escherichia coli, GI1786552, Length=375, Percent_Identity=58.4, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1788073, Length=372, Percent_Identity=27.4193548387097, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787863, Length=365, Percent_Identity=23.2876712328767, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082125, Length=388, Percent_Identity=25, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI226510992, Length=369, Percent_Identity=24.9322493224932, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786825, Length=381, Percent_Identity=24.4094488188976, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081918, Length=372, Percent_Identity=26.3440860215054, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=379, Percent_Identity=53.2981530343008, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71997431, Length=381, Percent_Identity=53.0183727034121, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=53.6443148688047, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17562584, Length=378, Percent_Identity=26.1904761904762, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=374, Percent_Identity=24.5989304812834, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562582, Length=382, Percent_Identity=24.869109947644, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320033, Length=373, Percent_Identity=54.9597855227882, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322619, Length=359, Percent_Identity=27.0194986072423, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319621, Length=379, Percent_Identity=27.4406332453826, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=359, Percent_Identity=27.0194986072423, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323099, Length=375, Percent_Identity=24.8, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319257, Length=379, Percent_Identity=24.5382585751979, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=280, Percent_Identity=28.2142857142857, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=372, Percent_Identity=25, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=366, Percent_Identity=24.5901639344262, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=359, Percent_Identity=25.3481894150418, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17737895, Length=374, Percent_Identity=58.0213903743316, Blast_Score=451, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 40007; Mature: 40007

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRAAVAVQAGKPLEVMTVQLEGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVVDVGPGVTSVKKGDHVIPLYTPECRECYSCTSRKTNLCTSIRSTQGQGVMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
GTSRFSIGKDKIHHYMGCSTFSNFTVLPEIALAKVNPDAPFDKICYIGCGVTTGIGAVIN
CCCEECCCHHHHHHHHCCCCCCCCEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHEE
TAKVEIGATAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
EEEEEECCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEECCCHHH
VGDDIVPYLVNLTKRHGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQ
HCCHHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
EISTRPFQLVTGRNWMGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINKGF
CCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCEEEECCEEEECCCHHHHHHHH
ELMHKGESIRGVVVY
HHHHCCCCCEEEEEC
>Mature Secondary Structure
MDVRAAVAVQAGKPLEVMTVQLEGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVVDVGPGVTSVKKGDHVIPLYTPECRECYSCTSRKTNLCTSIRSTQGQGVMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
GTSRFSIGKDKIHHYMGCSTFSNFTVLPEIALAKVNPDAPFDKICYIGCGVTTGIGAVIN
CCCEECCCHHHHHHHHCCCCCCCCEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHEE
TAKVEIGATAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
EEEEEECCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEECCCHHH
VGDDIVPYLVNLTKRHGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQ
HCCHHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
EISTRPFQLVTGRNWMGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINKGF
CCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCEEEECCEEEECCCHHHHHHHH
ELMHKGESIRGVVVY
HHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]