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The map label for this gene is nac [H]

Identifier: 116249076

GI number: 116249076

Start: 443128

End: 444066

Strand: Direct

Name: nac [H]

Synonym: pRL120410

Alternate gene names: 116249076

Gene position: 443128-444066 (Clockwise)

Preceding gene: 116249075

Following gene: 116249077

Centisome position: 50.93

GC content: 61.13

Gene sequence:

>939_bases
ATGAGCGTCGATTTCCGAAAACTGAAGAGCTTCGTCAAGATCGTCGATACAGGCAGCGTATCGCGAGCAGCAAGCCTTCT
GAGGACGGCCCAGCCGGCACTGTCGCAGCAGATTGCCGCTCTCGAAGCGCACTTCAAGCACAAGCTTCTCATCCGCAGCA
ATGTCGGCATTACGCCGACCGAGGCGGGGCTCATCCTCTATCGGCATGCGCAACTGATGCTGAAGCAGATCGAGCAGGCG
CAGGTCGACATCGATCAGGCCGCCAAATCGGTGGCGGGCCGCGTCTCGATCGGGCTTGCGACCTATTCCTCGTCAAGCGC
CTTGTCGCTGCCGATCCTCAAGGAGATGAAGGCCAGGCATCCCCATATCGTCGTCCACATCAATGACAGTTTCGGCCAGA
TCCTCAGCGAACTGATCATGACGGGCAAGATGGATATGGCGCTGATCTATGCCGCTGCCCCGATCAAGGGCGTCACCCTG
CAGCCATTGTTTCGCGAGGAAATGTTCCTTGTCTCGCCGCCGGGAATCGAACTTCCCGGCGAGAGCAGTGCGCCGCTTCC
CCTCTCTGCCCTCCAGGATATTCCGCTGCTGCTTCCCAGCAAGGCACATCTGCTCCGGCGGCTGATCGACGACGCCCTGG
CGAGAGCGAGAGCCACACCTGATGTCGTCGCGGAAATCGAATCCGTTCCGGCCCTCAGCGCGGCCGTCCTCGATGGGCTT
GGATCGACCATTCTGCCGGCTTCGGTGGTCACCGAGACTGCGATCTTTTCAGGCGCGCAGGTGCGGGCGCTGACGAAGCC
GGTGATTGACGCGACCGTGTCGCTTTGCGTCGCCGATCATCTTCCCTTGTCGGAACCCGCCATCGCTGCGCGGTCGGTTC
TCCTCGATGTCGTGGGCAAGCTCATGAGCAGTCATCACCCCGGAATCAGGCCGGCTTAG

Upstream 100 bases:

>100_bases
GCTGTTGCCGACCTGTATTGACTGCAAAGAGGCTGGCGGGGCCTTGCGCCCCACCGGCCGCAGGCTTTACGTTTTTTCGT
GAAACAAGAAGCCGGTGGCA

Downstream 100 bases:

>100_bases
CCCGCGGCCAATCCTCGCAACGGCGATCTGACGGCGTATCTCCGCTTCGCCATAAGCAAACCCTATAGCGGCAGACTGTA
GTTGTGTTAGCCAACGCGCC

Product: LysR family transcriptional regulatory protein

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSVDFRKLKSFVKIVDTGSVSRAASLLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPTEAGLILYRHAQLMLKQIEQA
QVDIDQAAKSVAGRVSIGLATYSSSSALSLPILKEMKARHPHIVVHINDSFGQILSELIMTGKMDMALIYAAAPIKGVTL
QPLFREEMFLVSPPGIELPGESSAPLPLSALQDIPLLLPSKAHLLRRLIDDALARARATPDVVAEIESVPALSAAVLDGL
GSTILPASVVTETAIFSGAQVRALTKPVIDATVSLCVADHLPLSEPAIAARSVLLDVVGKLMSSHHPGIRPA

Sequences:

>Translated_312_residues
MSVDFRKLKSFVKIVDTGSVSRAASLLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPTEAGLILYRHAQLMLKQIEQA
QVDIDQAAKSVAGRVSIGLATYSSSSALSLPILKEMKARHPHIVVHINDSFGQILSELIMTGKMDMALIYAAAPIKGVTL
QPLFREEMFLVSPPGIELPGESSAPLPLSALQDIPLLLPSKAHLLRRLIDDALARARATPDVVAEIESVPALSAAVLDGL
GSTILPASVVTETAIFSGAQVRALTKPVIDATVSLCVADHLPLSEPAIAARSVLLDVVGKLMSSHHPGIRPA
>Mature_311_residues
SVDFRKLKSFVKIVDTGSVSRAASLLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPTEAGLILYRHAQLMLKQIEQAQ
VDIDQAAKSVAGRVSIGLATYSSSSALSLPILKEMKARHPHIVVHINDSFGQILSELIMTGKMDMALIYAAAPIKGVTLQ
PLFREEMFLVSPPGIELPGESSAPLPLSALQDIPLLLPSKAHLLRRLIDDALARARATPDVVAEIESVPALSAAVLDGLG
STILPASVVTETAIFSGAQVRALTKPVIDATVSLCVADHLPLSEPAIAARSVLLDVVGKLMSSHHPGIRPA

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=296, Percent_Identity=38.1756756756757, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787601, Length=283, Percent_Identity=27.5618374558304, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI157672245, Length=272, Percent_Identity=28.3088235294118, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=28.7449392712551, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=25, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787806, Length=252, Percent_Identity=24.2063492063492, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787879, Length=256, Percent_Identity=25.78125, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789506, Length=245, Percent_Identity=23.265306122449, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790399, Length=275, Percent_Identity=24.7272727272727, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788481, Length=255, Percent_Identity=22.3529411764706, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=24.390243902439, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33138; Mature: 33007

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDFRKLKSFVKIVDTGSVSRAASLLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
EAGLILYRHAQLMLKQIEQAQVDIDQAAKSVAGRVSIGLATYSSSSALSLPILKEMKARH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEECHHHHHHHCCC
PHIVVHINDSFGQILSELIMTGKMDMALIYAAAPIKGVTLQPLFREEMFLVSPPGIELPG
CEEEEEECCHHHHHHHHHHHHCCCHHEEEEEECCCCCCEECHHHHCCEEEECCCCCCCCC
ESSAPLPLSALQDIPLLLPSKAHLLRRLIDDALARARATPDVVAEIESVPALSAAVLDGL
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHC
GSTILPASVVTETAIFSGAQVRALTKPVIDATVSLCVADHLPLSEPAIAARSVLLDVVGK
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LMSSHHPGIRPA
HHHCCCCCCCCC
>Mature Secondary Structure 
SVDFRKLKSFVKIVDTGSVSRAASLLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPT
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
EAGLILYRHAQLMLKQIEQAQVDIDQAAKSVAGRVSIGLATYSSSSALSLPILKEMKARH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEECHHHHHHHCCC
PHIVVHINDSFGQILSELIMTGKMDMALIYAAAPIKGVTLQPLFREEMFLVSPPGIELPG
CEEEEEECCHHHHHHHHHHHHCCCHHEEEEEECCCCCCEECHHHHCCEEEECCCCCCCCC
ESSAPLPLSALQDIPLLLPSKAHLLRRLIDDALARARATPDVVAEIESVPALSAAVLDGL
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHC
GSTILPASVVTETAIFSGAQVRALTKPVIDATVSLCVADHLPLSEPAIAARSVLLDVVGK
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LMSSHHPGIRPA
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]