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The map label for this gene is aatA [H]

Identifier: 116249075

GI number: 116249075

Start: 441840

End: 443048

Strand: Direct

Name: aatA [H]

Synonym: pRL120409

Alternate gene names: 116249075

Gene position: 441840-443048 (Clockwise)

Preceding gene: 116249074

Following gene: 116249076

Centisome position: 50.78

GC content: 60.79

Gene sequence:

>1209_bases
ATGAGCACTTTTGTGCCTGCATCGCGTGTGTCCAGAATCAAGGTTTCGCCAAGCACGGCTGCGGCAGCCCGGGCTCGCGA
ACTGAAGGCAGCCGGCCGCGATATCGTCGACCTGACCGTCGGCGAGCCGGACTTCGATACGCCTGACAACATCAAGGCCG
CGGCGCACGCGGCCATCGACAGGGGCGAAACCAAATATACCGCCGTCAACGGCACGCCGGCGCTGCGCAAGGCAATCATC
GGCGATATCAAGCGCCGGCTCGGCCTCAGCTACGCAGACAACGAAATCTGCGTGGGTGGCGGCGCCAAGCAGATTCTGTT
TCTGGCGCTTATGGCGAGCGTGGAAAACAATGCCGAGGTGATCATCCCCGCACCATACTGGGTCTCCTATCCCGATATGG
TCATCGCCAACGAAGGCAAGCCGGTCATTATCGAATGCCCACAGGAAACAGGCTTCAAGCTCACGCCGGAGGCTTTGGAA
AGAGCGATCACGCCGAAGACCTTGTGGCTGGTTCTCAACGCGCCGTCCAATCCAACGGGCGCGGCATATGACAGGAGCGA
GCTCGAAGCCCTCGGCAAAGTGCTGCTGCGCCATCCCCACGTCTTCGTGCTTTCCGACGACATTTACGACCAGGTCTGGT
TCAAGGACGAGCCGATGACGACGCTGGTGGCCGCCGTCCCGGAACTGAAGGAGCGTGTTCTGCTCACCAACGGCGTCTCA
AAGTCCTACGCCATGACCGGATGGCGTATCGGCTACGCGGCGGGTCCGGCCGTCTTGATCGCAGCCATCAACAAGCTGCA
GTCGCAGATGTCGTCCTGCCCGTCGTCGGTCAGTCAGGCCGCAGCCGCCTATGCGCTGTCTTCGGACCAGTCATTCGTCA
CGGAAAGCGTGACGGTCTACAGAAAGCGCCGGGATTATGCGTGCTCACGGCTCAACGCGGTGCCAGGCCTCTCCTGCCTG
GTGCCTGACGGCGCTTTCTATCTGTTTCCGAACTGCGCTGGTGTTATCGGCAAGAAGACCCCCGAAGGTAAGACAATCGA
GACAGACCTCGATTTCGTGCTCTATCTGCTCGATGGCGTCGGGGTCGCGGCGCTGCAGGGTGCCGCCTACGGAGTATCGC
CTCATTTCCGTCTCTCGATCGCAACGTCGATGGAGGCAATCACGCAGGCATGCGATCGCATTGAACGGGCTGTTGCCGAC
CTGTATTGA

Upstream 100 bases:

>100_bases
AGCTCGGCGAACTGTTCCTGACCGGGAAGATGCGCGGCGAAACCGAAGCCGTTCACTGAGGCGGTCCCGCCTCTCAAGAA
TCATAATCGAGGAAATCTCA

Downstream 100 bases:

>100_bases
CTGCAAAGAGGCTGGCGGGGCCTTGCGCCCCACCGGCCGCAGGCTTTACGTTTTTTCGTGAAACAAGAAGCCGGTGGCAA
TGAGCGTCGATTTCCGAAAA

Product: aspartate aminotransferase

Products: NA

Alternate protein names: AspAT; Transaminase A [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSTFVPASRVSRIKVSPSTAAAARARELKAAGRDIVDLTVGEPDFDTPDNIKAAAHAAIDRGETKYTAVNGTPALRKAII
GDIKRRLGLSYADNEICVGGGAKQILFLALMASVENNAEVIIPAPYWVSYPDMVIANEGKPVIIECPQETGFKLTPEALE
RAITPKTLWLVLNAPSNPTGAAYDRSELEALGKVLLRHPHVFVLSDDIYDQVWFKDEPMTTLVAAVPELKERVLLTNGVS
KSYAMTGWRIGYAAGPAVLIAAINKLQSQMSSCPSSVSQAAAAYALSSDQSFVTESVTVYRKRRDYACSRLNAVPGLSCL
VPDGAFYLFPNCAGVIGKKTPEGKTIETDLDFVLYLLDGVGVAALQGAAYGVSPHFRLSIATSMEAITQACDRIERAVAD
LY

Sequences:

>Translated_402_residues
MSTFVPASRVSRIKVSPSTAAAARARELKAAGRDIVDLTVGEPDFDTPDNIKAAAHAAIDRGETKYTAVNGTPALRKAII
GDIKRRLGLSYADNEICVGGGAKQILFLALMASVENNAEVIIPAPYWVSYPDMVIANEGKPVIIECPQETGFKLTPEALE
RAITPKTLWLVLNAPSNPTGAAYDRSELEALGKVLLRHPHVFVLSDDIYDQVWFKDEPMTTLVAAVPELKERVLLTNGVS
KSYAMTGWRIGYAAGPAVLIAAINKLQSQMSSCPSSVSQAAAAYALSSDQSFVTESVTVYRKRRDYACSRLNAVPGLSCL
VPDGAFYLFPNCAGVIGKKTPEGKTIETDLDFVLYLLDGVGVAALQGAAYGVSPHFRLSIATSMEAITQACDRIERAVAD
LY
>Mature_401_residues
STFVPASRVSRIKVSPSTAAAARARELKAAGRDIVDLTVGEPDFDTPDNIKAAAHAAIDRGETKYTAVNGTPALRKAIIG
DIKRRLGLSYADNEICVGGGAKQILFLALMASVENNAEVIIPAPYWVSYPDMVIANEGKPVIIECPQETGFKLTPEALER
AITPKTLWLVLNAPSNPTGAAYDRSELEALGKVLLRHPHVFVLSDDIYDQVWFKDEPMTTLVAAVPELKERVLLTNGVSK
SYAMTGWRIGYAAGPAVLIAAINKLQSQMSSCPSSVSQAAAAYALSSDQSFVTESVTVYRKRRDYACSRLNAVPGLSCLV
PDGAFYLFPNCAGVIGKKTPEGKTIETDLDFVLYLLDGVGVAALQGAAYGVSPHFRLSIATSMEAITQACDRIERAVADL
Y

Specific function: Required for symbiotic nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI95147551, Length=399, Percent_Identity=27.5689223057644, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI169881279, Length=399, Percent_Identity=27.5689223057644, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI56713254, Length=361, Percent_Identity=26.0387811634349, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI56713256, Length=361, Percent_Identity=26.0387811634349, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI169881281, Length=396, Percent_Identity=24.2424242424242, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI4507369, Length=421, Percent_Identity=23.7529691211401, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI19263340, Length=369, Percent_Identity=24.1192411924119, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI215599424, Length=369, Percent_Identity=24.1192411924119, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786816, Length=398, Percent_Identity=27.1356783919598, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788627, Length=413, Percent_Identity=26.1501210653753, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1788722, Length=409, Percent_Identity=29.0953545232274, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994472, Length=364, Percent_Identity=26.6483516483516, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71994476, Length=349, Percent_Identity=26.9340974212034, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17567369, Length=292, Percent_Identity=25.6849315068493, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567663, Length=407, Percent_Identity=22.6044226044226, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71981209, Length=404, Percent_Identity=22.5247524752475, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322401, Length=369, Percent_Identity=24.6612466124661, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323118, Length=403, Percent_Identity=23.3250620347395, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28573069, Length=337, Percent_Identity=24.3323442136499, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24646114, Length=337, Percent_Identity=24.3323442136499, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28573067, Length=337, Percent_Identity=24.3323442136499, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28573065, Length=337, Percent_Identity=24.3323442136499, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18859735, Length=379, Percent_Identity=23.2189973614776, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.1 [H]

Molecular weight: Translated: 43147; Mature: 43016

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFVPASRVSRIKVSPSTAAAARARELKAAGRDIVDLTVGEPDFDTPDNIKAAAHAAID
CCCCCCCHHCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHC
RGETKYTAVNGTPALRKAIIGDIKRRLGLSYADNEICVGGGAKQILFLALMASVENNAEV
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEE
IIPAPYWVSYPDMVIANEGKPVIIECPQETGFKLTPEALERAITPKTLWLVLNAPSNPTG
EEECCCEECCCCEEEECCCCEEEEECCCCCCCEECHHHHHHCCCCEEEEEEEECCCCCCC
AAYDRSELEALGKVLLRHPHVFVLSDDIYDQVWFKDEPMTTLVAAVPELKERVLLTNGVS
CCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHEECCCCHHHHHHHHHHHHHHEEHCCCCC
KSYAMTGWRIGYAAGPAVLIAAINKLQSQMSSCPSSVSQAAAAYALSSDQSFVTESVTVY
CCCEECCEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHH
RKRRDYACSRLNAVPGLSCLVPDGAFYLFPNCAGVIGKKTPEGKTIETDLDFVLYLLDGV
HHHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCC
GVAALQGAAYGVSPHFRLSIATSMEAITQACDRIERAVADLY
CHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STFVPASRVSRIKVSPSTAAAARARELKAAGRDIVDLTVGEPDFDTPDNIKAAAHAAID
CCCCCCHHCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHC
RGETKYTAVNGTPALRKAIIGDIKRRLGLSYADNEICVGGGAKQILFLALMASVENNAEV
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEE
IIPAPYWVSYPDMVIANEGKPVIIECPQETGFKLTPEALERAITPKTLWLVLNAPSNPTG
EEECCCEECCCCEEEECCCCEEEEECCCCCCCEECHHHHHHCCCCEEEEEEEECCCCCCC
AAYDRSELEALGKVLLRHPHVFVLSDDIYDQVWFKDEPMTTLVAAVPELKERVLLTNGVS
CCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHEECCCCHHHHHHHHHHHHHHEEHCCCCC
KSYAMTGWRIGYAAGPAVLIAAINKLQSQMSSCPSSVSQAAAAYALSSDQSFVTESVTVY
CCCEECCEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHH
RKRRDYACSRLNAVPGLSCLVPDGAFYLFPNCAGVIGKKTPEGKTIETDLDFVLYLLDGV
HHHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCC
GVAALQGAAYGVSPHFRLSIATSMEAITQACDRIERAVADLY
CHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2019560; 8096210; 11481430 [H]