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The map label for this gene is livM [H]

Identifier: 116249072

GI number: 116249072

Start: 439309

End: 440295

Strand: Direct

Name: livM [H]

Synonym: pRL120406

Alternate gene names: 116249072

Gene position: 439309-440295 (Clockwise)

Preceding gene: 116249071

Following gene: 116249073

Centisome position: 50.49

GC content: 60.49

Gene sequence:

>987_bases
ATGTCTCTCACGGAAACCGCTCAACAGGCACGCAGGCGCGACCTTCGCATACCGCTCGCCGCAGTCCTGGTCGTCCTGGC
ACTTGCCGCGCCGTTCATCGGCAGCCGTTACATCACCCATTCCCTGATCATCGCCCTGATCTTCATGCTTCCGGCTCACG
GGCTCAATCTGCTTGTGGGATACACCGGTCTTCTGTCATTGGCTCAGGCCGCGTTTTTCGGCATCGGCGCCTATACGGCA
GGTCTTCTCGCCGTCACCTACGGAACGCCGTTCTACGTCAATCTCATCGCCGCGGGCGCCGTCTCCGGGGCCCTTGCTCT
TCCGCTCGGCATTCCCGCCTTGCGTCTGCGCTCGACGTCCTTCGTCATGTGTACGCTCGGTTTCGTGATCATCGGCCAGG
CAATCGCCAAGAACTGGGTCTCGCTGACGCGCGGCGACATGGGACTATCGAGCATTCCAAAACCATACTTCGAGCTCGGC
CCGCTTTCGTTCACGGTGTCCGGTACGACGAACTTCTACTATCTTGCCCTGATTATCGGGACGCTTGCCACCTTCGCCGT
CTGGGCAATCGTCCGCTCGCCGGCCGGGCGCAATATGGTGGCGATCCGTGAGAACGAAACGCTCGCCGAATCTCTCGGCG
TTCCCACCTGGCACTACAAGCTGATCGTCTTCATGATCAGCGCCGTGTTTGCCGGGGTCGGCGGCTGCCTCTACGCCTAT
TACCTGACGGTCGTCAGCCCGCTGACCTTCCAGATGTACTATTCGACCACGATGCTGATCATCGTGCTCGGAGGCGGGGC
GGGGATGATTTCCGGCACTGTCTTCGGAAGCCTGCTCTTCGTCGGGCTGACGGAGGCGCTGCGCATTACACCCGAACTGC
GCATGATCGCCTATGGTGTCTGCCTTCTCGTCCTCGTCTTCTGGTTCAAGAAGGGCTGCGCGCCGCTGATCAATCGTTTC
TGGAATGCGATTGGAGGCGCGAAATGA

Upstream 100 bases:

>100_bases
GATCCTGATGCTGATGTGGCGACCGCATGGCCTCTTTTCGACGCAGGCGCGCTTCTAGCAGCTGCGCCCGCCATCTTTCT
CGAACGACCGAGGACTCGAT

Downstream 100 bases:

>100_bases
CCAGTCATCTTCCGCTTCTCGAGATCAAGAACCTCAGCAAGTCCTACGGTGCGGTCAAGGCCGTCAACGATGTCAGCATT
CACATCGATCGAGGTGAGAT

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSLTETAQQARRRDLRIPLAAVLVVLALAAPFIGSRYITHSLIIALIFMLPAHGLNLLVGYTGLLSLAQAAFFGIGAYTA
GLLAVTYGTPFYVNLIAAGAVSGALALPLGIPALRLRSTSFVMCTLGFVIIGQAIAKNWVSLTRGDMGLSSIPKPYFELG
PLSFTVSGTTNFYYLALIIGTLATFAVWAIVRSPAGRNMVAIRENETLAESLGVPTWHYKLIVFMISAVFAGVGGCLYAY
YLTVVSPLTFQMYYSTTMLIIVLGGGAGMISGTVFGSLLFVGLTEALRITPELRMIAYGVCLLVLVFWFKKGCAPLINRF
WNAIGGAK

Sequences:

>Translated_328_residues
MSLTETAQQARRRDLRIPLAAVLVVLALAAPFIGSRYITHSLIIALIFMLPAHGLNLLVGYTGLLSLAQAAFFGIGAYTA
GLLAVTYGTPFYVNLIAAGAVSGALALPLGIPALRLRSTSFVMCTLGFVIIGQAIAKNWVSLTRGDMGLSSIPKPYFELG
PLSFTVSGTTNFYYLALIIGTLATFAVWAIVRSPAGRNMVAIRENETLAESLGVPTWHYKLIVFMISAVFAGVGGCLYAY
YLTVVSPLTFQMYYSTTMLIIVLGGGAGMISGTVFGSLLFVGLTEALRITPELRMIAYGVCLLVLVFWFKKGCAPLINRF
WNAIGGAK
>Mature_327_residues
SLTETAQQARRRDLRIPLAAVLVVLALAAPFIGSRYITHSLIIALIFMLPAHGLNLLVGYTGLLSLAQAAFFGIGAYTAG
LLAVTYGTPFYVNLIAAGAVSGALALPLGIPALRLRSTSFVMCTLGFVIIGQAIAKNWVSLTRGDMGLSSIPKPYFELGP
LSFTVSGTTNFYYLALIIGTLATFAVWAIVRSPAGRNMVAIRENETLAESLGVPTWHYKLIVFMISAVFAGVGGCLYAYY
LTVVSPLTFQMYYSTTMLIIVLGGGAGMISGTVFGSLLFVGLTEALRITPELRMIAYGVCLLVLVFWFKKGCAPLINRFW
NAIGGAK

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=307, Percent_Identity=32.8990228013029, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35076; Mature: 34945

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTETAQQARRRDLRIPLAAVLVVLALAAPFIGSRYITHSLIIALIFMLPAHGLNLLVG
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YTGLLSLAQAAFFGIGAYTAGLLAVTYGTPFYVNLIAAGAVSGALALPLGIPALRLRSTS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCHHHHCCCH
FVMCTLGFVIIGQAIAKNWVSLTRGDMGLSSIPKPYFELGPLSFTVSGTTNFYYLALIIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHH
TLATFAVWAIVRSPAGRNMVAIRENETLAESLGVPTWHYKLIVFMISAVFAGVGGCLYAY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLTVVSPLTFQMYYSTTMLIIVLGGGAGMISGTVFGSLLFVGLTEALRITPELRMIAYGV
HHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
CLLVLVFWFKKGCAPLINRFWNAIGGAK
HHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLTETAQQARRRDLRIPLAAVLVVLALAAPFIGSRYITHSLIIALIFMLPAHGLNLLVG
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YTGLLSLAQAAFFGIGAYTAGLLAVTYGTPFYVNLIAAGAVSGALALPLGIPALRLRSTS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCHHHHCCCH
FVMCTLGFVIIGQAIAKNWVSLTRGDMGLSSIPKPYFELGPLSFTVSGTTNFYYLALIIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHH
TLATFAVWAIVRSPAGRNMVAIRENETLAESLGVPTWHYKLIVFMISAVFAGVGGCLYAY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLTVVSPLTFQMYYSTTMLIIVLGGGAGMISGTVFGSLLFVGLTEALRITPELRMIAYGV
HHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
CLLVLVFWFKKGCAPLINRFWNAIGGAK
HHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]