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The map label for this gene is braD [H]

Identifier: 116249071

GI number: 116249071

Start: 438394

End: 439266

Strand: Direct

Name: braD [H]

Synonym: pRL120405

Alternate gene names: 116249071

Gene position: 438394-439266 (Clockwise)

Preceding gene: 116249070

Following gene: 116249072

Centisome position: 50.39

GC content: 59.45

Gene sequence:

>873_bases
ATGCTCTCGACCATACTCGAGCAATCTGTGAATGGAATCGTCACGGGTTCCGTTTACGCCATCGTCGCCGTCGGCATGAC
CATGATTTTCGGCGTGCTGAGAGCCATCAATTTTGCTCACGGCGAATACTACATGCTCGGCACGTTCGGGGCCTGGTTTG
CGATCGATTATCTCGGCCTGTCCTACGAAGTGTCGATTGTCGTGGGCGTTCTTGCGACAATCGCCGTTGCCTATGTGGTC
GGGCAACTCGTCATGCGCCGGATGGTCGGCGCGCCCGCGGAGTCGGGCGTGCTCGCCACCCTTGGCATCGCGCTGATCCT
GCAGAACACCATCATTCTCGTCTTCGGCGGAGGCTACAAGTTCTTCGCCGGAGGCTATATCGAACCGGTTTCCATCCTGG
GCTTCAGTCTGGCCGAGCAGCGCATTCTCATTCTCGTCGTCTGCCTGTTGGTATTCATCGGCCTCGAACTGATGGTCAGC
TACACCCGCCTCGGAATGGCGATGCGCGCCGTTTCCCAGAACGTCGAGTGCTGCAGCGTCGTCGGCATCGATGTGCCACA
GGTGGTGCTTCGAACCTTCATGCTGGGGGCGGCCCTCGCCGCACTTTCGGGTGTTCTGACGGCGCCGGTCAATGTCAGTG
TTTATGGCGGCATGGGCGAGTTGATCACCTTCAAGACGCTGCCGATCATTATCATGGGCGGTTTGGGGAATGTGCGTGGA
ACTTTCGTCGCGGCGATGATCCTTGGCGTTGCCGAGAGCCTGGTCGCCACCTATGTCGGCTTGCAATTCCGCGACACGGT
CGGCTTCGCCACGCTGATCCTGATGCTGATGTGGCGACCGCATGGCCTCTTTTCGACGCAGGCGCGCTTCTAG

Upstream 100 bases:

>100_bases
GATCCTACTGCAGATCGAGAACGGCAAGCCGAGCATCAAGGGTGCATATACCGCGGACATTCAATATCCTGCCAACTAAT
CTGCCGGGAGGAGTAGGCCC

Downstream 100 bases:

>100_bases
CAGCTGCGCCCGCCATCTTTCTCGAACGACCGAGGACTCGATATGTCTCTCACGGAAACCGCTCAACAGGCACGCAGGCG
CGACCTTCGCATACCGCTCG

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MLSTILEQSVNGIVTGSVYAIVAVGMTMIFGVLRAINFAHGEYYMLGTFGAWFAIDYLGLSYEVSIVVGVLATIAVAYVV
GQLVMRRMVGAPAESGVLATLGIALILQNTIILVFGGGYKFFAGGYIEPVSILGFSLAEQRILILVVCLLVFIGLELMVS
YTRLGMAMRAVSQNVECCSVVGIDVPQVVLRTFMLGAALAALSGVLTAPVNVSVYGGMGELITFKTLPIIIMGGLGNVRG
TFVAAMILGVAESLVATYVGLQFRDTVGFATLILMLMWRPHGLFSTQARF

Sequences:

>Translated_290_residues
MLSTILEQSVNGIVTGSVYAIVAVGMTMIFGVLRAINFAHGEYYMLGTFGAWFAIDYLGLSYEVSIVVGVLATIAVAYVV
GQLVMRRMVGAPAESGVLATLGIALILQNTIILVFGGGYKFFAGGYIEPVSILGFSLAEQRILILVVCLLVFIGLELMVS
YTRLGMAMRAVSQNVECCSVVGIDVPQVVLRTFMLGAALAALSGVLTAPVNVSVYGGMGELITFKTLPIIIMGGLGNVRG
TFVAAMILGVAESLVATYVGLQFRDTVGFATLILMLMWRPHGLFSTQARF
>Mature_290_residues
MLSTILEQSVNGIVTGSVYAIVAVGMTMIFGVLRAINFAHGEYYMLGTFGAWFAIDYLGLSYEVSIVVGVLATIAVAYVV
GQLVMRRMVGAPAESGVLATLGIALILQNTIILVFGGGYKFFAGGYIEPVSILGFSLAEQRILILVVCLLVFIGLELMVS
YTRLGMAMRAVSQNVECCSVVGIDVPQVVLRTFMLGAALAALSGVLTAPVNVSVYGGMGELITFKTLPIIIMGGLGNVRG
TFVAAMILGVAESLVATYVGLQFRDTVGFATLILMLMWRPHGLFSTQARF

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=298, Percent_Identity=30.5369127516779, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 30776; Mature: 30776

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTILEQSVNGIVTGSVYAIVAVGMTMIFGVLRAINFAHGEYYMLGTFGAWFAIDYLGL
CHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCC
SYEVSIVVGVLATIAVAYVVGQLVMRRMVGAPAESGVLATLGIALILQNTIILVFGGGYK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCCEE
FFAGGYIEPVSILGFSLAEQRILILVVCLLVFIGLELMVSYTRLGMAMRAVSQNVECCSV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGIDVPQVVLRTFMLGAALAALSGVLTAPVNVSVYGGMGELITFKTLPIIIMGGLGNVRG
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHEEEEEECCCCCHHH
TFVAAMILGVAESLVATYVGLQFRDTVGFATLILMLMWRPHGLFSTQARF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLSTILEQSVNGIVTGSVYAIVAVGMTMIFGVLRAINFAHGEYYMLGTFGAWFAIDYLGL
CHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCC
SYEVSIVVGVLATIAVAYVVGQLVMRRMVGAPAESGVLATLGIALILQNTIILVFGGGYK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCCEE
FFAGGYIEPVSILGFSLAEQRILILVVCLLVFIGLELMVSYTRLGMAMRAVSQNVECCSV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGIDVPQVVLRTFMLGAALAALSGVLTAPVNVSVYGGMGELITFKTLPIIIMGGLGNVRG
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHEEEEEECCCCCHHH
TFVAAMILGVAESLVATYVGLQFRDTVGFATLILMLMWRPHGLFSTQARF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]