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The map label for this gene is braC [H]

Identifier: 116249070

GI number: 116249070

Start: 437179

End: 438372

Strand: Direct

Name: braC [H]

Synonym: pRL120404

Alternate gene names: 116249070

Gene position: 437179-438372 (Clockwise)

Preceding gene: 116249069

Following gene: 116249071

Centisome position: 50.25

GC content: 58.88

Gene sequence:

>1194_bases
ATGAAACTTACCGTAAGCCGCAGAGCACTTTTCATTTCTACAGCGGTCCTTTCGCTGGCCGTGACCGTCTCCGGCCTGCC
GGCTATCGCGCTGGCAGCGGACGGGACGATCAAGCTCGGACTTGTCGCGCCGATGAGCGGTCCGAATGCGCGCTACGGCG
CATTTTCAATGCATGGAGCCGAACTCGCCGTCAAAGACATCAATGATGCCGGCGGCATCGACGGGATGAAAATCGAACTC
CTCAACGCCGACAGCCAGGGCACGCCGGTCGAAGGCGTTTCGGCCGCCCGTCGTTTGATTGACCAGGATGGCGTCGACTT
CATCATCGGCGATGTTTCCAGCTCGGTGACGCTTGCCATCCAGCCCGTCGCGGAAGATGCCGAAGTGCTTCTCCTGAATG
CGGCCTCATCCAATCCGAAGATCACCTATCAGGCCGGTGTCGGCGGTTTCAAGTGGACCTATCGCAACTATCCGACAGAT
GAGAACCGGGCGCTGATCGTCGCCAAATATGCCGCAGAACAGCGCGGCTTCACCAAGTTCGCGGTGCTCTCGGTCGACAG
CGACTATGGCCGCTCGGCGATCACCTTCACGAAGAAGTACCTGCCCGATTTCAAGGGTGAGATCCTCAGCGAAGACTATT
ACAAGGAAGGCGAAGTCGACTTCCGCAGCGTTCTCGCCAAGATCCGCGACAATGGCGCCCAGGCGATCATCATGTACGGC
CTCGCCGATACGACGCCCATCATCGCCCGCCAGATGATCGAACTCGGAATAGCCGGCAAGGTCGCGCTGATCGGCAATGG
CGAGTTCAACACGGAAAAGACCATCAAATCAGCCCCGTCGGCGCTCGAAGGTGCCGTCGAAGCCGCCGCATGGCTTCCGC
AGTTCGACTCGCCTGAAAGCAAGGCTTTCGTCGAGAAGTTCACTGCCACCTACAACGAAGCCCCGAACAACCACGCCTAC
GTTCACTGGGATACCGTCAACCTCCTCGCCGCGGCGATCAAGCAGGCCGGCAGTGCTGACAAGGTGAAGGTTCGCGATGC
ACTCTCGAAGATCAAGTACAAGAGCCCGGTCGGCGAAGTCACTTTCGACGACCACAACCAGGCCCGCCTGCCGATGATCC
TACTGCAGATCGAGAACGGCAAGCCGAGCATCAAGGGTGCATATACCGCGGACATTCAATATCCTGCCAACTAA

Upstream 100 bases:

>100_bases
GTGTCAGCGCTGGGCTGACGCGATAAGAACCGCGGAAAGCGGTCAGGACTACGCTGGGCCATATGCCAAGCGTTTGCAAC
CGAGAACATGGAGTGGGAAC

Downstream 100 bases:

>100_bases
TCTGCCGGGAGGAGTAGGCCCATGCTCTCGACCATACTCGAGCAATCTGTGAATGGAATCGTCACGGGTTCCGTTTACGC
CATCGTCGCCGTCGGCATGA

Product: putative substrate-binding component of ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKLTVSRRALFISTAVLSLAVTVSGLPAIALAADGTIKLGLVAPMSGPNARYGAFSMHGAELAVKDINDAGGIDGMKIEL
LNADSQGTPVEGVSAARRLIDQDGVDFIIGDVSSSVTLAIQPVAEDAEVLLLNAASSNPKITYQAGVGGFKWTYRNYPTD
ENRALIVAKYAAEQRGFTKFAVLSVDSDYGRSAITFTKKYLPDFKGEILSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
LADTTPIIARQMIELGIAGKVALIGNGEFNTEKTIKSAPSALEGAVEAAAWLPQFDSPESKAFVEKFTATYNEAPNNHAY
VHWDTVNLLAAAIKQAGSADKVKVRDALSKIKYKSPVGEVTFDDHNQARLPMILLQIENGKPSIKGAYTADIQYPAN

Sequences:

>Translated_397_residues
MKLTVSRRALFISTAVLSLAVTVSGLPAIALAADGTIKLGLVAPMSGPNARYGAFSMHGAELAVKDINDAGGIDGMKIEL
LNADSQGTPVEGVSAARRLIDQDGVDFIIGDVSSSVTLAIQPVAEDAEVLLLNAASSNPKITYQAGVGGFKWTYRNYPTD
ENRALIVAKYAAEQRGFTKFAVLSVDSDYGRSAITFTKKYLPDFKGEILSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
LADTTPIIARQMIELGIAGKVALIGNGEFNTEKTIKSAPSALEGAVEAAAWLPQFDSPESKAFVEKFTATYNEAPNNHAY
VHWDTVNLLAAAIKQAGSADKVKVRDALSKIKYKSPVGEVTFDDHNQARLPMILLQIENGKPSIKGAYTADIQYPAN
>Mature_397_residues
MKLTVSRRALFISTAVLSLAVTVSGLPAIALAADGTIKLGLVAPMSGPNARYGAFSMHGAELAVKDINDAGGIDGMKIEL
LNADSQGTPVEGVSAARRLIDQDGVDFIIGDVSSSVTLAIQPVAEDAEVLLLNAASSNPKITYQAGVGGFKWTYRNYPTD
ENRALIVAKYAAEQRGFTKFAVLSVDSDYGRSAITFTKKYLPDFKGEILSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
LADTTPIIARQMIELGIAGKVALIGNGEFNTEKTIKSAPSALEGAVEAAAWLPQFDSPESKAFVEKFTATYNEAPNNHAY
VHWDTVNLLAAAIKQAGSADKVKVRDALSKIKYKSPVGEVTFDDHNQARLPMILLQIENGKPSIKGAYTADIQYPAN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=362, Percent_Identity=24.0331491712707, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI48994941, Length=201, Percent_Identity=30.3482587064677, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42532; Mature: 42532

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTVSRRALFISTAVLSLAVTVSGLPAIALAADGTIKLGLVAPMSGPNARYGAFSMHGA
CEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCCCCCEEEECCC
ELAVKDINDAGGIDGMKIELLNADSQGTPVEGVSAARRLIDQDGVDFIIGDVSSSVTLAI
EEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEE
QPVAEDAEVLLLNAASSNPKITYQAGVGGFKWTYRNYPTDENRALIVAKYAAEQRGFTKF
EECCCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCCCCEEE
AVLSVDSDYGRSAITFTKKYLPDFKGEILSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
EEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
LADTTPIIARQMIELGIAGKVALIGNGEFNTEKTIKSAPSALEGAVEAAAWLPQFDSPES
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCH
KAFVEKFTATYNEAPNNHAYVHWDTVNLLAAAIKQAGSADKVKVRDALSKIKYKSPVGEV
HHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCE
TFDDHNQARLPMILLQIENGKPSIKGAYTADIQYPAN
EECCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MKLTVSRRALFISTAVLSLAVTVSGLPAIALAADGTIKLGLVAPMSGPNARYGAFSMHGA
CEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCCCCCEEEECCC
ELAVKDINDAGGIDGMKIELLNADSQGTPVEGVSAARRLIDQDGVDFIIGDVSSSVTLAI
EEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEE
QPVAEDAEVLLLNAASSNPKITYQAGVGGFKWTYRNYPTDENRALIVAKYAAEQRGFTKF
EECCCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCCCCEEE
AVLSVDSDYGRSAITFTKKYLPDFKGEILSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
EEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
LADTTPIIARQMIELGIAGKVALIGNGEFNTEKTIKSAPSALEGAVEAAAWLPQFDSPES
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCH
KAFVEKFTATYNEAPNNHAYVHWDTVNLLAAAIKQAGSADKVKVRDALSKIKYKSPVGEV
HHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCE
TFDDHNQARLPMILLQIENGKPSIKGAYTADIQYPAN
EECCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]