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The map label for this gene is dctA [H]

Identifier: 116248943

GI number: 116248943

Start: 288229

End: 289554

Strand: Reverse

Name: dctA [H]

Synonym: pRL120273

Alternate gene names: 116248943

Gene position: 289554-288229 (Counterclockwise)

Preceding gene: 116248948

Following gene: 116248942

Centisome position: 33.28

GC content: 59.28

Gene sequence:

>1326_bases
ATGACACCTGTGGGTGAGACGCAAAAACCGGCGGGCCGATTTGGCCTCTTTGGCCAGTTGTGGTTTCAGGTGCTGGCAGG
GACGCTGCTCGGCATCCTGCTGGGTCATTTCGCTCCCGCCACTGCGGTGGCGATGAAACCTTTCGGCGACGCCTTCATAA
AAATGATCCGCATGATGATCGGGCCGATCATCTTCGTCACCGTTGTGCATGGTATCGCCGGCATGAACGACATGAAGAGC
GTCGGGCGTGTCGCGCTGAAATCCATTATCTATTTCGAGGCGATCACGATCTTGGCGCTGATCTTCGGACTGATCGCAGT
CGATCTGTGGCGTCCGGGCGAGGGCATGAACATCGACGCTACCGCAATCGATACCGCATCGATCAAGACCTATCTTGCGA
CGGCGCACGACCAGTCCATTTCAAGCTATTTCCTTGACATCATCCCAGACACCTTCGCCAGCGCGCTGACGGAAGGACAC
GTTCTGCAGGTCCTGTTTATCTCTGTCCTGTTCGGTATCGCCATCGCCGCCATCGGAGAACGCGGACGCCCCGTGTTCCA
GGTGATCGAAAGCGCCTCCCAAGGGTTTTTCAAGATCATTGGCTACATCATGTACTTCGCGCCGCTCGGGGCCTTTGGCG
CAATTGCCTTTACCGTCGGTCAGTTCGGCACCGCCTCGCTCTGGTCGCTGGGCGAGTTAATCATCGAGTTCTTCGTCGTC
TGCGCCTTGTTCACAACAATCGTCCTCGGCAGCGTTGCCCATTGGTGCGGTGTCAGCCTGTGGCGGCTGCTAGGCTATCT
CTGGGATGAAATCGTCATCGTCGCTGCGACCACCTCGACCGAAACGGTGCTGCCGCGCCTCATCCAGAAGATGCGCAAGG
CCGGCTGTGAGGAAAGCGTGGTCGGCTTCGTCATCCCGGCCGGCTATTCGTTCAACCTCGACGGCACATGCCTCTATCTG
ACCACCGTCGCTGTGTTCCTGGCACAGGCGACGAACACACATCTGACCTTCTGGCACGAACTCGGCCTGATCGCGGTGTT
GCTGCTCACCTCGAAGGGAGCGGCGGGCATCGCCGGAGCGGCCTTCGTTGTACTGGCGGCAACCCTGAATACCACCGGAA
CCATTCCGGTCGCCAGCATCGCGCTGATCCTCGGAATTCACCGTATCCTTGCGGAGGGGCTGACTTTTGTGAATCTGATC
GGCAATGCGCTTGCCACCGTCGTCGTCGCGAAATGGGAAGGCAAGCTTGACGAGCAGCTACTGGCCGCCACTATCGGCAA
CGACCGCTTGCGCGCCAGGCACCAAACCGCCGCCGCTGCGTTGTGA

Upstream 100 bases:

>100_bases
CGGTGCAGTTGCCATTGAACAATTGAAAGATGGGACATCATGGCCGGGCAGGCAGCTTGGGAGGAAATAAAACATGACGT
ATTTGGAGGCGAGTAAAACG

Downstream 100 bases:

>100_bases
CATGGTGCAAGACTCAGCTCCGGTCTGCGATCTATCCTATTGGAAGCCGAATTGTCGCATAGGTTCAGGTCCTGTGACAA
AATTACGGGACGCCACCATG

Product: putative aerobic c4-dicarboxylate transport protein

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTPVGETQKPAGRFGLFGQLWFQVLAGTLLGILLGHFAPATAVAMKPFGDAFIKMIRMMIGPIIFVTVVHGIAGMNDMKS
VGRVALKSIIYFEAITILALIFGLIAVDLWRPGEGMNIDATAIDTASIKTYLATAHDQSISSYFLDIIPDTFASALTEGH
VLQVLFISVLFGIAIAAIGERGRPVFQVIESASQGFFKIIGYIMYFAPLGAFGAIAFTVGQFGTASLWSLGELIIEFFVV
CALFTTIVLGSVAHWCGVSLWRLLGYLWDEIVIVAATTSTETVLPRLIQKMRKAGCEESVVGFVIPAGYSFNLDGTCLYL
TTVAVFLAQATNTHLTFWHELGLIAVLLLTSKGAAGIAGAAFVVLAATLNTTGTIPVASIALILGIHRILAEGLTFVNLI
GNALATVVVAKWEGKLDEQLLAATIGNDRLRARHQTAAAAL

Sequences:

>Translated_441_residues
MTPVGETQKPAGRFGLFGQLWFQVLAGTLLGILLGHFAPATAVAMKPFGDAFIKMIRMMIGPIIFVTVVHGIAGMNDMKS
VGRVALKSIIYFEAITILALIFGLIAVDLWRPGEGMNIDATAIDTASIKTYLATAHDQSISSYFLDIIPDTFASALTEGH
VLQVLFISVLFGIAIAAIGERGRPVFQVIESASQGFFKIIGYIMYFAPLGAFGAIAFTVGQFGTASLWSLGELIIEFFVV
CALFTTIVLGSVAHWCGVSLWRLLGYLWDEIVIVAATTSTETVLPRLIQKMRKAGCEESVVGFVIPAGYSFNLDGTCLYL
TTVAVFLAQATNTHLTFWHELGLIAVLLLTSKGAAGIAGAAFVVLAATLNTTGTIPVASIALILGIHRILAEGLTFVNLI
GNALATVVVAKWEGKLDEQLLAATIGNDRLRARHQTAAAAL
>Mature_440_residues
TPVGETQKPAGRFGLFGQLWFQVLAGTLLGILLGHFAPATAVAMKPFGDAFIKMIRMMIGPIIFVTVVHGIAGMNDMKSV
GRVALKSIIYFEAITILALIFGLIAVDLWRPGEGMNIDATAIDTASIKTYLATAHDQSISSYFLDIIPDTFASALTEGHV
LQVLFISVLFGIAIAAIGERGRPVFQVIESASQGFFKIIGYIMYFAPLGAFGAIAFTVGQFGTASLWSLGELIIEFFVVC
ALFTTIVLGSVAHWCGVSLWRLLGYLWDEIVIVAATTSTETVLPRLIQKMRKAGCEESVVGFVIPAGYSFNLDGTCLYLT
TVAVFLAQATNTHLTFWHELGLIAVLLLTSKGAAGIAGAAFVVLAATLNTTGTIPVASIALILGIHRILAEGLTFVNLIG
NALATVVVAKWEGKLDEQLLAATIGNDRLRARHQTAAAAL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=477, Percent_Identity=23.4800838574423, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI194239697, Length=471, Percent_Identity=23.1422505307856, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI66773030, Length=432, Percent_Identity=22.6851851851852, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21314632, Length=403, Percent_Identity=22.0843672456576, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4827012, Length=309, Percent_Identity=24.9190938511327, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI40254478, Length=463, Percent_Identity=22.6781857451404, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI262359914, Length=375, Percent_Identity=23.2, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI223468564, Length=264, Percent_Identity=23.1060606060606, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI223468566, Length=264, Percent_Identity=23.1060606060606, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI5032093, Length=410, Percent_Identity=22.6829268292683, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789947, Length=415, Percent_Identity=50.3614457831325, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=40.0479616306954, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1788024, Length=403, Percent_Identity=21.0918114143921, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71996953, Length=430, Percent_Identity=26.2790697674419, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17537407, Length=424, Percent_Identity=24.7641509433962, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=407, Percent_Identity=24.5700245700246, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=473, Percent_Identity=21.7758985200846, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71983099, Length=473, Percent_Identity=21.7758985200846, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193206654, Length=445, Percent_Identity=20.8988764044944, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17541374, Length=431, Percent_Identity=20.8816705336427, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583025, Length=419, Percent_Identity=27.4463007159904, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17137668, Length=419, Percent_Identity=27.4463007159904, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583023, Length=419, Percent_Identity=27.4463007159904, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281360481, Length=483, Percent_Identity=22.9813664596273, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281360483, Length=483, Percent_Identity=22.9813664596273, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137666, Length=483, Percent_Identity=22.9813664596273, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46986; Mature: 46855

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPVGETQKPAGRFGLFGQLWFQVLAGTLLGILLGHFAPATAVAMKPFGDAFIKMIRMMI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
GPIIFVTVVHGIAGMNDMKSVGRVALKSIIYFEAITILALIFGLIAVDLWRPGEGMNIDA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
TAIDTASIKTYLATAHDQSISSYFLDIIPDTFASALTEGHVLQVLFISVLFGIAIAAIGE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
RGRPVFQVIESASQGFFKIIGYIMYFAPLGAFGAIAFTVGQFGTASLWSLGELIIEFFVV
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
CALFTTIVLGSVAHWCGVSLWRLLGYLWDEIVIVAATTSTETVLPRLIQKMRKAGCEESV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHCCCCCCC
VGFVIPAGYSFNLDGTCLYLTTVAVFLAQATNTHLTFWHELGLIAVLLLTSKGAAGIAGA
EEEEEECCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
AFVVLAATLNTTGTIPVASIALILGIHRILAEGLTFVNLIGNALATVVVAKWEGKLDEQL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAATIGNDRLRARHQTAAAAL
HHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA