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The map label for this gene is budB [H]

Identifier: 116248941

GI number: 116248941

Start: 285111

End: 286757

Strand: Reverse

Name: budB [H]

Synonym: pRL120271

Alternate gene names: 116248941

Gene position: 286757-285111 (Counterclockwise)

Preceding gene: 116248942

Following gene: 116248940

Centisome position: 32.96

GC content: 61.02

Gene sequence:

>1647_bases
ATGACTAAAGGCTCCGATCTTCTAGTGGCAGCGATTGAGAACGAAGGTGTCGATCGCATCTTCGGCATCCCAGGCGAAGA
GAATCTCGACGTCGTCGAATCCATCCGCAAGTCGTCGATTGAGCTCGTCCTCACCCGACACGAGCAGGCAGCAGCGTTCA
TGGCCGCGACCTATGGCCGCCTGACTGGAAAGCCCGGCGTCTGCCTGACGACGCTTGGCCCCGGCGCTCTGAACCTCTCG
ACGGGTGCCGCCTATGCCTTGCTCGGCGCGATGCCGATGGTGATGATCACCGGTCAGAAGGGGATCCTCTCGTCCCGCCA
GGCACGTTTCCAGGTGGTCGACGTCGTCGCGTCGATGAAGCCGCTGACCAAGCTCGCAAGGCAGATCGTGTCGCCGCAGA
TGATCCCGACGACGGTCAGGGAAGCGTTCCGCATCGCCCAGGAGGAAAAGCCGGGGCCGGTGCATCTGGAGCTTCCGGAA
GATATTGCAGCCGAGGAATGCCAGGAGGTGGCGCTGATTGCACCGCACCAGCTCGAACTGCCAACGGCAAGCGACGCGGC
CCTTGACCGTGCCGCCGACCTCATCGCCGCAGCCAAACGTCCGCTTCTGATGTTCGGGGCCGCCGCCTCGCGTCCCCGCT
CGACATCAGATATTGCGCAGTTTGTGATCCGTACGCGCATTCCGTTCTTTACCACTCAGATGGGGAAGGGAACGGTTCCC
GGCGGAACCGAACTTTATATGGGCACCGCCGCGCTGTCGGAGCGGGATTATGTCCACGAGGCCATCGAACAGGCAGACCT
TATCATCACGATCGGGCACGACACCATCGAGAAGCCACCGTTCATCATGGGCAAGGGCGGCCCCAAGGTCGTTCATGTCG
GATATCAGCCGGCGACCGTCGAGCAGGTCTACTTCCCGCAATCCGAAGTCATCGGCGACATCGGCCCTTCGCTGAAGGCG
CTGGCGGATCGCCTCGAGGGCAAGCTGCCAAACGCGCATGCGCTTCTCCATCTCCGCGAGCGCATTCTCGAGCGCATCGC
CGCGCGGGCGACGGAGGATCGCTTCACGCCACAGCGGCTGGTTCACGATATAAGAGAGGTGATGCCGCATGATGGCATCC
TGGCCCTCGACAACGGCATGTACAAGATCTGGTTCGCCCGCAACTACCGGACGCGGATGGCGAACACGCTGCTGCTCGAC
AATGCTCTTGCAACGATGGGAGCGGGACTGCCGTCGGCCATGGTTGCCTCGATGCTCTACCCTGAGCGGCGTGTCATGGC
GATCTGCGGTGACGGCGGCTTCATGATGAATTCCCAGGAACTCGAAACCGCCGTCCGGCTGAAACTCAACCTCGTCGTTC
TTGTCATCGAGGACAATGCTTACGGGATGATCCGTTGGAAGCAGGCCGTGGACGAATTCCCGGATTTCGGGATGACCTTC
GGCAATCCCGACTTCGTGAAATATGCAGAATCCTACGGCGCCAGGGGAACCAGGGTCGAGGATATCAGCCAGTTCAAACA
GGTTCTCGAAGAGGCATTCTCCGGAGGTGGCGTCCATCTGGTGAACGTCCCGGTCGACTACTCGGAAAATGAACGCGTGC
TGGTAAAGGAGCTTCGTGAACGGCTCCCCGCGATATTGGAGGCCTGA

Upstream 100 bases:

>100_bases
CCGATGCCGCCGAACGCATGGACAGGCCCGGAAAAGAACATATCTTCAAGGGGCCTTCCTTTCTGGAAGCCTCGAACCAA
ACAGCACGGGCACACATCCA

Downstream 100 bases:

>100_bases
GATGCCGCCTGCCATGAAGGTCCACAGGCGTTCGACCGCGAGCCGCACGATTGCCGGCACACGACGCGGCACGATGGAAC
AGATACCTATCTTCTTGAAC

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTKGSDLLVAAIENEGVDRIFGIPGEENLDVVESIRKSSIELVLTRHEQAAAFMAATYGRLTGKPGVCLTTLGPGALNLS
TGAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASMKPLTKLARQIVSPQMIPTTVREAFRIAQEEKPGPVHLELPE
DIAAEECQEVALIAPHQLELPTASDAALDRAADLIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTTQMGKGTVP
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKGGPKVVHVGYQPATVEQVYFPQSEVIGDIGPSLKA
LADRLEGKLPNAHALLHLRERILERIAARATEDRFTPQRLVHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLD
NALATMGAGLPSAMVASMLYPERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIRWKQAVDEFPDFGMTF
GNPDFVKYAESYGARGTRVEDISQFKQVLEEAFSGGGVHLVNVPVDYSENERVLVKELRERLPAILEA

Sequences:

>Translated_548_residues
MTKGSDLLVAAIENEGVDRIFGIPGEENLDVVESIRKSSIELVLTRHEQAAAFMAATYGRLTGKPGVCLTTLGPGALNLS
TGAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASMKPLTKLARQIVSPQMIPTTVREAFRIAQEEKPGPVHLELPE
DIAAEECQEVALIAPHQLELPTASDAALDRAADLIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTTQMGKGTVP
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKGGPKVVHVGYQPATVEQVYFPQSEVIGDIGPSLKA
LADRLEGKLPNAHALLHLRERILERIAARATEDRFTPQRLVHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLD
NALATMGAGLPSAMVASMLYPERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIRWKQAVDEFPDFGMTF
GNPDFVKYAESYGARGTRVEDISQFKQVLEEAFSGGGVHLVNVPVDYSENERVLVKELRERLPAILEA
>Mature_547_residues
TKGSDLLVAAIENEGVDRIFGIPGEENLDVVESIRKSSIELVLTRHEQAAAFMAATYGRLTGKPGVCLTTLGPGALNLST
GAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASMKPLTKLARQIVSPQMIPTTVREAFRIAQEEKPGPVHLELPED
IAAEECQEVALIAPHQLELPTASDAALDRAADLIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTTQMGKGTVPG
GTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKGGPKVVHVGYQPATVEQVYFPQSEVIGDIGPSLKAL
ADRLEGKLPNAHALLHLRERILERIAARATEDRFTPQRLVHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLDN
ALATMGAGLPSAMVASMLYPERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIRWKQAVDEFPDFGMTFG
NPDFVKYAESYGARGTRVEDISQFKQVLEEAFSGGGVHLVNVPVDYSENERVLVKELRERLPAILEA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=558, Percent_Identity=25.6272401433692, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI93004078, Length=554, Percent_Identity=24.0072202166065, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=28.5714285714286, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=27.124773960217, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=26.4044943820225, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1787096, Length=526, Percent_Identity=26.0456273764259, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788716, Length=484, Percent_Identity=26.2396694214876, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=540, Percent_Identity=27.2222222222222, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=540, Percent_Identity=27.2222222222222, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=578, Percent_Identity=25.4325259515571, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=561, Percent_Identity=29.9465240641711, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=22.7941176470588, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323073, Length=477, Percent_Identity=24.1090146750524, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=475, Percent_Identity=24.2105263157895, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323163, Length=473, Percent_Identity=24.1014799154334, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922626, Length=489, Percent_Identity=26.3803680981595, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59916; Mature: 59785

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGSDLLVAAIENEGVDRIFGIPGEENLDVVESIRKSSIELVLTRHEQAAAFMAATYGR
CCCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHH
LTGKPGVCLTTLGPGALNLSTGAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASMK
CCCCCCEEEEECCCCCEECCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
PLTKLARQIVSPQMIPTTVREAFRIAQEEKPGPVHLELPEDIAAEECQEVALIAPHQLEL
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHEEEECCCEECC
PTASDAALDRAADLIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTTQMGKGTVP
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCC
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKGGPKVVHVGYQPATV
CCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCH
EQVYFPQSEVIGDIGPSLKALADRLEGKLPNAHALLHLRERILERIAARATEDRFTPQRL
HEEECCHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLDNALATMGAGLPSAMVASMLY
HHHHHHHCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIRWKQAVDEFPDFGMTF
CCCEEEEEECCCCEEECHHHHHHHHEEEEEEEEEEEECCCCEEEEEHHHHHCCCCCCCCC
GNPDFVKYAESYGARGTRVEDISQFKQVLEEAFSGGGVHLVNVPVDYSENERVLVKELRE
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
RLPAILEA
HHHHHHCC
>Mature Secondary Structure 
TKGSDLLVAAIENEGVDRIFGIPGEENLDVVESIRKSSIELVLTRHEQAAAFMAATYGR
CCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHH
LTGKPGVCLTTLGPGALNLSTGAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASMK
CCCCCCEEEEECCCCCEECCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
PLTKLARQIVSPQMIPTTVREAFRIAQEEKPGPVHLELPEDIAAEECQEVALIAPHQLEL
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHEEEECCCEECC
PTASDAALDRAADLIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTTQMGKGTVP
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCC
GGTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKGGPKVVHVGYQPATV
CCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCH
EQVYFPQSEVIGDIGPSLKALADRLEGKLPNAHALLHLRERILERIAARATEDRFTPQRL
HEEECCHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLDNALATMGAGLPSAMVASMLY
HHHHHHHCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIRWKQAVDEFPDFGMTF
CCCEEEEEECCCCEEECHHHHHHHHEEEEEEEEEEEECCCCEEEEEHHHHHCCCCCCCCC
GNPDFVKYAESYGARGTRVEDISQFKQVLEEAFSGGGVHLVNVPVDYSENERVLVKELRE
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
RLPAILEA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]