| Definition | Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence. |
|---|---|
| Accession | NC_008378 |
| Length | 870,021 |
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The map label for this gene is melA [H]
Identifier: 116248926
GI number: 116248926
Start: 267534
End: 268850
Strand: Reverse
Name: melA [H]
Synonym: pRL120256
Alternate gene names: 116248926
Gene position: 268850-267534 (Counterclockwise)
Preceding gene: 116248928
Following gene: 116248925
Centisome position: 30.9
GC content: 57.18
Gene sequence:
>1317_bases ATGCCGAAAATTTGCCTGGTAGGCGCTGGGAGCACCGTTTTTGCTCAGAACATTCTGGGAGATGTCCTGTCTTCGCAAAG AGGCGGCGACTATCTCATCAGCCTCTTCGATATCGATCCAGAGCGACTGAAGACTTCAGAGATCGTTGCTCGCCGGATCT GCGAGTCGCTTAAGCTTTCAAACGTCAGGATAGACGCCACGCTCGACCGGCGGGAAGCGCTGCGCGGTTCCGATTTCGTG ATCCTCATGATGCAGGTCGGCGGCTACAAGCCGGCGACGGTCACGGACTTCGACATTCCAAAAAAATATGGCTTGCGTCA GACGATCGCCGACACCCTTGGCATCGGCGGCATTTTCCGCGGTTTGCGGACGATACCAGTCCTTGAGGCGATCTGCCGAG ACATGCAGGAGGTCTGCCCACAGGCTCTTCTGATGCAGTACGTCAACCCGATGGCCATCAACTGCTGGGCGATCAAGGAT CTGGCCCCGGAAATCCGCACCGTCGGCTTGTGTCACAGTGTGCAGCACACAGCCGGGCATCTCGCGCAATGCCTCGGCGA GGACATTGCCGACGTGAACTATGTTTCGGCTGGGATCAACCACGTCGCCTTCTTCCTGAAATATGAGAAAATTCATAGCG ACGGACGGCGGGAAGACCTTTATCCGAGACTGAACGCTCTCGCCGCCGATGGCCGCGTTCCGCCGGACGATCGGGTGCGG TTCGATGTTCTCAAACGGCTCGGCCACTTCGTCACCGAATCCAGCGAGCATTTCTCCGAGTATACCTCCTGGTACATCAA GGAGGGACGAGGGGATCTGATCGATCAGCTCAATATTCCGTTGGACGAATACATCCGGCGCTGCGAGGTGCAGATCAAAG AGTGGCATGCCCTTCGCAAGGAACTGGAAGGCGACAAGCCGATCGAGGTGTGCCGCAGCAATGAATATGCGGCCGGCATC ATTCATGCCGCAATAACCGGCAACCCGGCGCTGATATACGGCAATGTCCCAAACAACGGCCTGATCGAGAATCTGCCCGA TGAATGCATCGTGGAAGTGCCTTGCCATGTCGACAGAAACGGAATTCAGCCGGTCCGGGTCGGTCGGATCCCCTCTCAGC TTTCCGCCGTCATGAACTTGAGCGTTTCCGTTCAGCAGTTGACGGTCGAGGCGGCTCTTACAAGAAACCGCGAGCGCATC TACCAGGCCGCTCTGCTCGATCCGCATACGTCCGCGGAATTGTCGCCGGACCAAATCTGGAACCTTGTCGACGACCTGAT CGTCGCACACGGCGATTTGTTGCCGAGATATCAGTGA
Upstream 100 bases:
>100_bases TTTGCAGAAGACTCGAATCCCGGAATTAATAGGCCGATGCCAGGAGGAAATTCTGATCTAGAAGGAGCAACAAGCAATCA CAACCCATGGGGAGATTGCA
Downstream 100 bases:
>100_bases GACCCCGCGGATAAGCCAGCCACAAGCGCAGGGCAGTTCATTTGTTTAGACGCGTCGTGAATTCACGGCGCGTTTTTTTC ATTCCAGCGTCATCCAATAA
Product: alpha-galactosidase (melibiase)
Products: NA
Alternate protein names: Melibiase [H]
Number of amino acids: Translated: 438; Mature: 437
Protein sequence:
>438_residues MPKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLSNVRIDATLDRREALRGSDFV ILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFRGLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKD LAPEIRTVGLCHSVQHTAGHLAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRKELEGDKPIEVCRSNEYAAGI IHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRNGIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERI YQAALLDPHTSAELSPDQIWNLVDDLIVAHGDLLPRYQ
Sequences:
>Translated_438_residues MPKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLSNVRIDATLDRREALRGSDFV ILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFRGLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKD LAPEIRTVGLCHSVQHTAGHLAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRKELEGDKPIEVCRSNEYAAGI IHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRNGIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERI YQAALLDPHTSAELSPDQIWNLVDDLIVAHGDLLPRYQ >Mature_437_residues PKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLSNVRIDATLDRREALRGSDFVI LMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFRGLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKDL APEIRTVGLCHSVQHTAGHLAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVRF DVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRKELEGDKPIEVCRSNEYAAGII HAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRNGIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERIY QAALLDPHTSAELSPDQIWNLVDDLIVAHGDLLPRYQ
Specific function: Unknown
COG id: COG1486
COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 4 family [H]
Homologues:
Organism=Escherichia coli, GI1790560, Length=445, Percent_Identity=51.9101123595506, Blast_Score=464, Evalue=1e-132, Organism=Escherichia coli, GI1788029, Length=460, Percent_Identity=25, Blast_Score=107, Evalue=2e-24,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR019802 - InterPro: IPR001088 - InterPro: IPR022616 - InterPro: IPR015955 - InterPro: IPR016040 [H]
Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]
EC number: =3.2.1.22 [H]
Molecular weight: Translated: 48915; Mature: 48784
Theoretical pI: Translated: 5.61; Mature: 5.61
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.5 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 2.5 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLS CCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHC NVRIDATLDRREALRGSDFVILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFR CEEEEEEECHHHHCCCCCEEEEEEECCCCCCCEECCCCCCHHHCHHHHHHHHHCHHHHHH GLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKDLAPEIRTVGLCHSVQHTAGH HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH LAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHH FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRK HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH ELEGDKPIEVCRSNEYAAGIIHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRN HCCCCCCHHHHCCCCCHHEEEEEEECCCCEEEECCCCCCCCCCCCCCHHEEECCCCCCCC GIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERIYQAALLDPHTSAELSPDQIW CCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHH NLVDDLIVAHGDLLPRYQ HHHHHHHHHCCCCCCCCC >Mature Secondary Structure PKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLS CCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHC NVRIDATLDRREALRGSDFVILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFR CEEEEEEECHHHHCCCCCEEEEEEECCCCCCCEECCCCCCHHHCHHHHHHHHHCHHHHHH GLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKDLAPEIRTVGLCHSVQHTAGH HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH LAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHH FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRK HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH ELEGDKPIEVCRSNEYAAGIIHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRN HCCCCCCHHHHCCCCCHHEEEEEEECCCCEEEECCCCCCCCCCCCCCHHEEECCCCCCCC GIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERIYQAALLDPHTSAELSPDQIW CCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHH NLVDDLIVAHGDLLPRYQ HHHHHHHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]