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The map label for this gene is ilvD [H]

Identifier: 116248908

GI number: 116248908

Start: 248469

End: 250205

Strand: Reverse

Name: ilvD [H]

Synonym: pRL120238

Alternate gene names: 116248908

Gene position: 250205-248469 (Counterclockwise)

Preceding gene: 116248910

Following gene: 116248907

Centisome position: 28.76

GC content: 62.06

Gene sequence:

>1737_bases
GTGACCGCGAGGAAAACCTACGAGCAATTGCGGTCCGCCCGATGGATGCTGCCGGATGATCAGCGCTCGTTCGGCCACCG
GTCGCGGACCATGCAGATGGGTTACGCGCCGGAGGATTGGCAGGGAAAGCCGATCATTGCTGTGATCAACACCTGGTCCG
ATGCGCAGCCGTGCCACATGCATTTCCGTGAACGCGCCGAATGGGTGAAGCGGGGAATTCTTCAGTCGGGTGGGTTCCCC
ATGGAACTGCCCGCTCTTTCCCTTTCCGAAAACTTCGTCAAGCCGACCACGATGCTCTATCGCAATATGCTGGCGATGGA
GACCGAGGAACTGTTGCGGAGCCATCCTGTCGATGGTGCCGTTCTGATGGGTGGCTGCGACAAGACGACACCCGGCCTTG
TCATGGGCGCAGTCAGCATGGGCATTCCCTTCATTTATCTGCCGGCCGGCCCGATGCTTCGCGGCAACTATGCCGGCAAG
ACGCTGGGCTCCGGTACCGACGGCTTCAAATATTGGGACGAGCGCCGCGCCGGAACGATCACCCAGGAGGAGTGGCAGGG
CATCGAAGGCGGGATCGCCCGCAGTTACGGCCATTGCATGACCATGGGCACGGCGTCGACGATGACGGCGATCGCCGAGG
CTATGGGATTGACACTGCCGGGCGCATCCTCGATCCCTGCAGCCGACGCCAACCACCAGCGCATGTCGGCGGCCTGCGGC
CGCCGCATCGTCGATATGGTGTGGGAGGATCTGACGCCGGAGCAGATCATCACGTCCGCGGCCGTCGACAACGCAGTCAC
GGTCGCCATGGCGACCGGCTGCTCCACCAATGCCATCATTCACCTGATCGCCATGGCGCGGCGCGCCGGCGTCCCGCTGG
AGCTCGATGATCTCGATCGCATCGGTCGCACGACGCCGGTTCTCGCCAACATCCGCCCTTCCGGTTCGACCTACCTGATG
GAGGATTTCTTTTATGCGGGTGGCTTGCGCGCCCTGATGAAGCAACTCGGCGACAAGCTGGATCCCACCGCGATCACCGT
GACTGGAAAACCCTTGGTGGATGGTCTGGACCAGGTGAAGATCTACAATGACGACGTTATCCGGCCATTGTCGAACCCGG
TTTATCATGAAGGTTCGCTGGCCGTGCTCAAGGGGAACCTGTGTCCCGACGGCGCGGTCATCAAACCGGCGGCCTGCGAT
CCGAAATTCCATCGCCATCGCGGCCCGGCGCTGGTCGCCGACAGCTATGCGGAGATGAAGAAGATCATCGATGATCCCGA
CTATCCGCTGACGCCGGACACGGTTCTCGTGCTCCGCAATGCCGGCCCCCAAGGCGGACCGGGCATGCCGGAATGGGGCA
TGATCCCGATGCCGAAGGCGCTGTTGAAGCTCGGCCTGCGCGACATGGTGCGTATCTCGGACGCCCGCATGTCCGGAACC
AGTTTCGGTGCCTGCGTGCTGCATGTCGCACCGGAATCTTATGTTGGCGGGCCTTTGGCGCTGCTGAGAACGGGGGACAT
GGTCGAGCTTGATATTCCGGCACGCAGCCTCAATATGCTGGTTGCCGAAGAAGAGATCACAGCGCGGCGGGCCGCCTGGG
TGGCGCCGACGCGGCATTACGAGCGCGGTTACGGTTTTATGTTCTCCGGCCATATCGAACAGGCCGACAAAGGCTGCGAT
TTTGATTTCCTGACGACGGAATTCGGTGGCAAGACGCCGGAACCGGCCATCAACTGA

Upstream 100 bases:

>100_bases
AATGCACAAGAACGCAAACTTTCCATTGATATATATGTCAAACTAAAATATCAGTTGGTGCAAACGCTGAAATAAGCAAG
CGAAAAGGTGGGAGAAAGCC

Downstream 100 bases:

>100_bases
GGCACCACGATGTTTGATCCCGATCGACGTACGATTCCCGCACAGCCTCCCAAGGCTGGCTTCGCTGCCGGTCCGTCCAA
GTTGGACGCCATCCGATCCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MTARKTYEQLRSARWMLPDDQRSFGHRSRTMQMGYAPEDWQGKPIIAVINTWSDAQPCHMHFRERAEWVKRGILQSGGFP
MELPALSLSENFVKPTTMLYRNMLAMETEELLRSHPVDGAVLMGGCDKTTPGLVMGAVSMGIPFIYLPAGPMLRGNYAGK
TLGSGTDGFKYWDERRAGTITQEEWQGIEGGIARSYGHCMTMGTASTMTAIAEAMGLTLPGASSIPAADANHQRMSAACG
RRIVDMVWEDLTPEQIITSAAVDNAVTVAMATGCSTNAIIHLIAMARRAGVPLELDDLDRIGRTTPVLANIRPSGSTYLM
EDFFYAGGLRALMKQLGDKLDPTAITVTGKPLVDGLDQVKIYNDDVIRPLSNPVYHEGSLAVLKGNLCPDGAVIKPAACD
PKFHRHRGPALVADSYAEMKKIIDDPDYPLTPDTVLVLRNAGPQGGPGMPEWGMIPMPKALLKLGLRDMVRISDARMSGT
SFGACVLHVAPESYVGGPLALLRTGDMVELDIPARSLNMLVAEEEITARRAAWVAPTRHYERGYGFMFSGHIEQADKGCD
FDFLTTEFGGKTPEPAIN

Sequences:

>Translated_578_residues
MTARKTYEQLRSARWMLPDDQRSFGHRSRTMQMGYAPEDWQGKPIIAVINTWSDAQPCHMHFRERAEWVKRGILQSGGFP
MELPALSLSENFVKPTTMLYRNMLAMETEELLRSHPVDGAVLMGGCDKTTPGLVMGAVSMGIPFIYLPAGPMLRGNYAGK
TLGSGTDGFKYWDERRAGTITQEEWQGIEGGIARSYGHCMTMGTASTMTAIAEAMGLTLPGASSIPAADANHQRMSAACG
RRIVDMVWEDLTPEQIITSAAVDNAVTVAMATGCSTNAIIHLIAMARRAGVPLELDDLDRIGRTTPVLANIRPSGSTYLM
EDFFYAGGLRALMKQLGDKLDPTAITVTGKPLVDGLDQVKIYNDDVIRPLSNPVYHEGSLAVLKGNLCPDGAVIKPAACD
PKFHRHRGPALVADSYAEMKKIIDDPDYPLTPDTVLVLRNAGPQGGPGMPEWGMIPMPKALLKLGLRDMVRISDARMSGT
SFGACVLHVAPESYVGGPLALLRTGDMVELDIPARSLNMLVAEEEITARRAAWVAPTRHYERGYGFMFSGHIEQADKGCD
FDFLTTEFGGKTPEPAIN
>Mature_577_residues
TARKTYEQLRSARWMLPDDQRSFGHRSRTMQMGYAPEDWQGKPIIAVINTWSDAQPCHMHFRERAEWVKRGILQSGGFPM
ELPALSLSENFVKPTTMLYRNMLAMETEELLRSHPVDGAVLMGGCDKTTPGLVMGAVSMGIPFIYLPAGPMLRGNYAGKT
LGSGTDGFKYWDERRAGTITQEEWQGIEGGIARSYGHCMTMGTASTMTAIAEAMGLTLPGASSIPAADANHQRMSAACGR
RIVDMVWEDLTPEQIITSAAVDNAVTVAMATGCSTNAIIHLIAMARRAGVPLELDDLDRIGRTTPVLANIRPSGSTYLME
DFFYAGGLRALMKQLGDKLDPTAITVTGKPLVDGLDQVKIYNDDVIRPLSNPVYHEGSLAVLKGNLCPDGAVIKPAACDP
KFHRHRGPALVADSYAEMKKIIDDPDYPLTPDTVLVLRNAGPQGGPGMPEWGMIPMPKALLKLGLRDMVRISDARMSGTS
FGACVLHVAPESYVGGPLALLRTGDMVELDIPARSLNMLVAEEEITARRAAWVAPTRHYERGYGFMFSGHIEQADKGCDF
DFLTTEFGGKTPEPAIN

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=595, Percent_Identity=29.9159663865546, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788157, Length=545, Percent_Identity=28.256880733945, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI2367371, Length=457, Percent_Identity=31.7286652078775, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1786464, Length=456, Percent_Identity=29.6052631578947, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322476, Length=579, Percent_Identity=30.0518134715026, Blast_Score=268, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62951; Mature: 62820

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARKTYEQLRSARWMLPDDQRSFGHRSRTMQMGYAPEDWQGKPIIAVINTWSDAQPCHM
CCCHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCHHHH
HFRERAEWVKRGILQSGGFPMELPALSLSENFVKPTTMLYRNMLAMETEELLRSHPVDGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VLMGGCDKTTPGLVMGAVSMGIPFIYLPAGPMLRGNYAGKTLGSGTDGFKYWDERRAGTI
EEECCCCCCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCC
TQEEWQGIEGGIARSYGHCMTMGTASTMTAIAEAMGLTLPGASSIPAADANHQRMSAACG
CHHHHCCCCCCHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
RRIVDMVWEDLTPEQIITSAAVDNAVTVAMATGCSTNAIIHLIAMARRAGVPLELDDLDR
HHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHH
IGRTTPVLANIRPSGSTYLMEDFFYAGGLRALMKQLGDKLDPTAITVTGKPLVDGLDQVK
CCCCCCEEEEECCCCCEEHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCHHHCCCHHEE
IYNDDVIRPLSNPVYHEGSLAVLKGNLCPDGAVIKPAACDPKFHRHRGPALVADSYAEMK
EECCCHHCCCCCCCEECCCEEEEECCCCCCCCEECCCCCCCHHHHCCCCEEEHHHHHHHH
KIIDDPDYPLTPDTVLVLRNAGPQGGPGMPEWGMIPMPKALLKLGLRDMVRISDARMSGT
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCC
SFGACVLHVAPESYVGGPLALLRTGDMVELDIPARSLNMLVAEEEITARRAAWVAPTRHY
CCCEEEEEECCCCCCCCCEEEEECCCEEEEECCHHHHHEEEEHHHHHHHHHHHCCCHHHH
ERGYGFMFSGHIEQADKGCDFDFLTTEFGGKTPEPAIN
HCCCCEEEECCHHHHCCCCCCCEEEHHCCCCCCCCCCC
>Mature Secondary Structure 
TARKTYEQLRSARWMLPDDQRSFGHRSRTMQMGYAPEDWQGKPIIAVINTWSDAQPCHM
CCHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCHHHH
HFRERAEWVKRGILQSGGFPMELPALSLSENFVKPTTMLYRNMLAMETEELLRSHPVDGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VLMGGCDKTTPGLVMGAVSMGIPFIYLPAGPMLRGNYAGKTLGSGTDGFKYWDERRAGTI
EEECCCCCCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCC
TQEEWQGIEGGIARSYGHCMTMGTASTMTAIAEAMGLTLPGASSIPAADANHQRMSAACG
CHHHHCCCCCCHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
RRIVDMVWEDLTPEQIITSAAVDNAVTVAMATGCSTNAIIHLIAMARRAGVPLELDDLDR
HHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHH
IGRTTPVLANIRPSGSTYLMEDFFYAGGLRALMKQLGDKLDPTAITVTGKPLVDGLDQVK
CCCCCCEEEEECCCCCEEHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCHHHCCCHHEE
IYNDDVIRPLSNPVYHEGSLAVLKGNLCPDGAVIKPAACDPKFHRHRGPALVADSYAEMK
EECCCHHCCCCCCCEECCCEEEEECCCCCCCCEECCCCCCCHHHHCCCCEEEHHHHHHHH
KIIDDPDYPLTPDTVLVLRNAGPQGGPGMPEWGMIPMPKALLKLGLRDMVRISDARMSGT
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCC
SFGACVLHVAPESYVGGPLALLRTGDMVELDIPARSLNMLVAEEEITARRAAWVAPTRHY
CCCEEEEEECCCCCCCCCEEEEECCCEEEEECCHHHHHEEEEHHHHHHHHHHHCCCHHHH
ERGYGFMFSGHIEQADKGCDFDFLTTEFGGKTPEPAIN
HCCCCEEEECCHHHHCCCCCCCEEEHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA