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The map label for this gene is mdlA [H]

Identifier: 116248907

GI number: 116248907

Start: 247269

End: 248459

Strand: Reverse

Name: mdlA [H]

Synonym: pRL120237

Alternate gene names: 116248907

Gene position: 248459-247269 (Counterclockwise)

Preceding gene: 116248908

Following gene: 116248906

Centisome position: 28.56

GC content: 63.9

Gene sequence:

>1191_bases
ATGTTTGATCCCGATCGACGTACGATTCCCGCACAGCCTCCCAAGGCTGGCTTCGCTGCCGGTCCGTCCAAGTTGGACGC
CATCCGATCCGTCACCCTGTCGCTCGTTTACCTGCCTTTGGCGCGGCCGATCAGCGATGCCAAGGTTTTGACGGGCAGGC
AGAAGCCGCTGACGCAGGTGGCCTTTCTATTTTGCGAGATCGTCTCCGAGGCCGGCCATAGCGGTCTCGGTTTCAGTTAC
TCGAAGCGGGCAGGCGGACCTGCCCTTTATGCACACGCCTGCGAGCTTGCCGACAACCTGATCGGCGAAGACCCCAACGA
TACCGCGCGCATCTGGGACAAGCTCTGCTGGGCAGGGGCCTCGGTCGGCCGCTCCGGCATCGCAACGCAGGCAATCGCCG
CCATCGACATCTGCCTCTGGGACCTCAAGGCGAAACGCGCCGGCCTGCCGCTCGCAAAACTGCTTGGCGCCCATCGCGAC
AGTGTCGCCTGCTACAACACCTCCGGCGGCTTCCTGTCCTCGAGCGTCGAGGAAATCCGCGATGCCATCGACCACTCGAT
CGCCTCCGGTATCGGCGGCATCAAGATCAAGGTCGGACAGCCGGACCCGATGATCGACCTTCGCCGGCTTGATGCGGTGA
CCAGCCATATCGATGGCCGTGTCCCACTGATGGTCGATGCAAACCAGCAATGGGACCGCACGACGGCCTTGCGCTTCGGC
CGGCTGGTCGAACCGCTCAACCTCGAATGGATCGAAGAGCCGCTCGATGCCTATGACGCCGAGGGCCATGCGGCACTGGC
GCGCGAACTTGCCACACCGATCGCCACCGGCGAAATGCTGGCAAGCGCCGACGAACACATGGCGCTGATCCGGGCCGATG
CGGTCGACTTCATTCAGCCGGATGCGCCGCGCGTCGGCGGCATCACGCCCTTTCTGCGCATCTGCACGCACGCCGAGGCG
AAACGTATGCGCCTGGCTCCGCATTTCGCCATGGAAATCCATCTGCACCTGGCGGCCGCCTATGCGCATGAGCCGTGGGT
AGAGCATTTCGATTGGCTGGCGCCGCTGTTCAACGAAGAACTCGAGATCAAGGACGGCCGGATGATCGTGCCGGCCCGTC
CGGGGCTTGGTTGCAGCCTGACGGGCAAAGCCCGCGACTGGACCGTGGAAACGCGCAGTTTCGGCGTCTGA

Upstream 100 bases:

>100_bases
CGGCCATATCGAACAGGCCGACAAAGGCTGCGATTTTGATTTCCTGACGACGGAATTCGGTGGCAAGACGCCGGAACCGG
CCATCAACTGAGGCACCACG

Downstream 100 bases:

>100_bases
ATGAGAACGAGGAGAAGGAAATATGAACCCCGCAACCCGTGAAAAGCTGATGGGTGTCTCCGTCGCGACGCTCTGTTCGG
CGCTGTTCAAGCGCGGCCTG

Product: putative mandelate racemase

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MFDPDRRTIPAQPPKAGFAAGPSKLDAIRSVTLSLVYLPLARPISDAKVLTGRQKPLTQVAFLFCEIVSEAGHSGLGFSY
SKRAGGPALYAHACELADNLIGEDPNDTARIWDKLCWAGASVGRSGIATQAIAAIDICLWDLKAKRAGLPLAKLLGAHRD
SVACYNTSGGFLSSSVEEIRDAIDHSIASGIGGIKIKVGQPDPMIDLRRLDAVTSHIDGRVPLMVDANQQWDRTTALRFG
RLVEPLNLEWIEEPLDAYDAEGHAALARELATPIATGEMLASADEHMALIRADAVDFIQPDAPRVGGITPFLRICTHAEA
KRMRLAPHFAMEIHLHLAAAYAHEPWVEHFDWLAPLFNEELEIKDGRMIVPARPGLGCSLTGKARDWTVETRSFGV

Sequences:

>Translated_396_residues
MFDPDRRTIPAQPPKAGFAAGPSKLDAIRSVTLSLVYLPLARPISDAKVLTGRQKPLTQVAFLFCEIVSEAGHSGLGFSY
SKRAGGPALYAHACELADNLIGEDPNDTARIWDKLCWAGASVGRSGIATQAIAAIDICLWDLKAKRAGLPLAKLLGAHRD
SVACYNTSGGFLSSSVEEIRDAIDHSIASGIGGIKIKVGQPDPMIDLRRLDAVTSHIDGRVPLMVDANQQWDRTTALRFG
RLVEPLNLEWIEEPLDAYDAEGHAALARELATPIATGEMLASADEHMALIRADAVDFIQPDAPRVGGITPFLRICTHAEA
KRMRLAPHFAMEIHLHLAAAYAHEPWVEHFDWLAPLFNEELEIKDGRMIVPARPGLGCSLTGKARDWTVETRSFGV
>Mature_396_residues
MFDPDRRTIPAQPPKAGFAAGPSKLDAIRSVTLSLVYLPLARPISDAKVLTGRQKPLTQVAFLFCEIVSEAGHSGLGFSY
SKRAGGPALYAHACELADNLIGEDPNDTARIWDKLCWAGASVGRSGIATQAIAAIDICLWDLKAKRAGLPLAKLLGAHRD
SVACYNTSGGFLSSSVEEIRDAIDHSIASGIGGIKIKVGQPDPMIDLRRLDAVTSHIDGRVPLMVDANQQWDRTTALRFG
RLVEPLNLEWIEEPLDAYDAEGHAALARELATPIATGEMLASADEHMALIRADAVDFIQPDAPRVGGITPFLRICTHAEA
KRMRLAPHFAMEIHLHLAAAYAHEPWVEHFDWLAPLFNEELEIKDGRMIVPARPGLGCSLTGKARDWTVETRSFGV

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=391, Percent_Identity=24.5524296675192, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI186972148, Length=313, Percent_Identity=25.5591054313099, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI42544117, Length=388, Percent_Identity=23.4536082474227, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787864, Length=368, Percent_Identity=26.6304347826087, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI48994953, Length=357, Percent_Identity=27.1708683473389, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI226510960, Length=344, Percent_Identity=25.8720930232558, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 42971; Mature: 42971

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPDRRTIPAQPPKAGFAAGPSKLDAIRSVTLSLVYLPLARPISDAKVLTGRQKPLTQV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHH
AFLFCEIVSEAGHSGLGFSYSKRAGGPALYAHACELADNLIGEDPNDTARIWDKLCWAGA
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCH
SVGRSGIATQAIAAIDICLWDLKAKRAGLPLAKLLGAHRDSVACYNTSGGFLSSSVEEIR
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
DAIDHSIASGIGGIKIKVGQPDPMIDLRRLDAVTSHIDGRVPLMVDANQQWDRTTALRFG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
RLVEPLNLEWIEEPLDAYDAEGHAALARELATPIATGEMLASADEHMALIRADAVDFIQP
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCC
DAPRVGGITPFLRICTHAEAKRMRLAPHFAMEIHLHLAAAYAHEPWVEHFDWLAPLFNEE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCC
LEIKDGRMIVPARPGLGCSLTGKARDWTVETRSFGV
EEECCCCEEEECCCCCCEEECCCCCCEEECCCCCCC
>Mature Secondary Structure
MFDPDRRTIPAQPPKAGFAAGPSKLDAIRSVTLSLVYLPLARPISDAKVLTGRQKPLTQV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHH
AFLFCEIVSEAGHSGLGFSYSKRAGGPALYAHACELADNLIGEDPNDTARIWDKLCWAGA
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCH
SVGRSGIATQAIAAIDICLWDLKAKRAGLPLAKLLGAHRDSVACYNTSGGFLSSSVEEIR
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
DAIDHSIASGIGGIKIKVGQPDPMIDLRRLDAVTSHIDGRVPLMVDANQQWDRTTALRFG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
RLVEPLNLEWIEEPLDAYDAEGHAALARELATPIATGEMLASADEHMALIRADAVDFIQP
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCC
DAPRVGGITPFLRICTHAEAKRMRLAPHFAMEIHLHLAAAYAHEPWVEHFDWLAPLFNEE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCC
LEIKDGRMIVPARPGLGCSLTGKARDWTVETRSFGV
EEECCCCEEEECCCCCCEEECCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]