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The map label for this gene is hbpA [H]

Identifier: 116248902

GI number: 116248902

Start: 242331

End: 243851

Strand: Reverse

Name: hbpA [H]

Synonym: pRL120232

Alternate gene names: 116248902

Gene position: 243851-242331 (Counterclockwise)

Preceding gene: 116248903

Following gene: 116248901

Centisome position: 28.03

GC content: 58.32

Gene sequence:

>1521_bases
ATGAAACTGCATTTGTTAGCAGCCTGCTTTTCAACAATGGTGCTCGCGCTGAGCGGCGGCACGGCTCAGGCACAGGATGC
CAAGAGCAACGTCACTGTTGTGCTTGCCGAAACCGTCGATGTCGTCGAACCCTGCATGGCAGCGCGCCAGGACGTCGGCC
GGGTCATTTCTGAAAACGTCAACGAGATGCTGGTGGAATTCGACTACGTCAATGGCGGCCTCAAGCCCCGCCTGGCGACG
GAATGGTCGAAGATCGACGACGACACCTGGGAGTTTAAGCTGCGCCCGAATGTCAAATGGCACGATGGCAAGCCGTTCAC
TGCCAAGGATGTCCAATTCACCATCGAGCGCAACAAGAATAAGAAGCTCAGCTGTGAGACCGGCGGCAAATATTTCGGCG
GCATGGAGTTCAGCTTCGAAACGCCGGATGCCAACACGATCCGCATTACGACAAAACCGGCGCAGCCGATTCTTCCGCTT
CTGATGACGGTGATGTCTGTGGAATCTGCCGAGGCGACACCAGCCGACGAATTCACCCGCAAGCCGATTGGCACCGGCCC
TTATACGTTCGACAAATGGGAAATCGGCCAGTCAATCGTGCTGAAACGCAATCCGGAATATTGGGGGGAGAAACCGCAGG
TCGAACAGGCGACCTATCTGTTCCGCTCCGACAGCGCCGTTGCTGCCGCCATGGTCGATGCTGGCGAAGCCGATATCGTT
CCGGCCGTATCCGTACAGGATGCCACCAACAAGGAAACCGATTTCGCCTACCCGAATTCGGAGACGACATCGCTGCGTAT
CGATACGCGCGCAGCCCCCCTTAACGACCGGCGCATCCGCGAAGCGATGAACCTCGCCATCGATCGTCAGGCGATGCTCG
GAACGTTGTTCCCGGAACAGGCAAAGATCGCGACACAGCTCGTCGTGCCGACCACGATCGGCTACAATGCCGATATCCCC
GCCTGGCCCTATGATCCCGAAAAAGCAAAGGAGCTGGTCGCAGCAGCAAAAGCGGACGGCGTCCCGATCGATCGTGAGAT
CCGCATCATCGGTCGCAACGGGCAATATCCCAACGCCACCGAAGCGATGGAAGCGATGATGGCGATGCTTCAGGAAGTCG
GCCTGAACGTAAAGCTCGACATGTATGACGTTTCCGTGTGGAACGGCTACTTCGTTGCACCCTTTGTCGCCGATTCCGGC
CCGACGCTGACTCAGTCGCAGCACGACAATGCAACCGGCGACCCTGTCTTCACCGCATTCGTGAAATACGCCACCGACGG
CTCCCACTCCATGGTTCGCGATCCCGCCGTCGACGCCCTGATCGCCAAGGCGACGTCTGCCACCGGCGACGAGCGCACAA
AACTCTGGAAGGAGCTGTTCGCCAAGGTGAACACCGAAATCATCGCCGACATCCCGATGTTTCACATGGTCGGTTTCACC
CGCGTCTCGCCGCGTCTCGACTTCAAGCCGACGATCGCGACGAATTCCGAACTGCAGCTGTCGCAGATCCGCTTCAAGTA
A

Upstream 100 bases:

>100_bases
TGTGCTTTATCAAGCTATTGAGGAGATAGATGCCGGAGCACCGGAAACGGGACGCAGATCTACTGTGAGGAGCGCCAAAG
GCGCATGGGAGGAGAACGAA

Downstream 100 bases:

>100_bases
ACCGGTATGCCGCACTTGAGCACGGGCCGCCGCAAATAGCGGCCCGTGCTTTCCATGACGTGAGGGAGAAATGCCTATGC
CGCTGATTGACCGCATTGCC

Product: putative exported substrate binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Hemin-binding lipoprotein [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKLHLLAACFSTMVLALSGGTAQAQDAKSNVTVVLAETVDVVEPCMAARQDVGRVISENVNEMLVEFDYVNGGLKPRLAT
EWSKIDDDTWEFKLRPNVKWHDGKPFTAKDVQFTIERNKNKKLSCETGGKYFGGMEFSFETPDANTIRITTKPAQPILPL
LMTVMSVESAEATPADEFTRKPIGTGPYTFDKWEIGQSIVLKRNPEYWGEKPQVEQATYLFRSDSAVAAAMVDAGEADIV
PAVSVQDATNKETDFAYPNSETTSLRIDTRAAPLNDRRIREAMNLAIDRQAMLGTLFPEQAKIATQLVVPTTIGYNADIP
AWPYDPEKAKELVAAAKADGVPIDREIRIIGRNGQYPNATEAMEAMMAMLQEVGLNVKLDMYDVSVWNGYFVAPFVADSG
PTLTQSQHDNATGDPVFTAFVKYATDGSHSMVRDPAVDALIAKATSATGDERTKLWKELFAKVNTEIIADIPMFHMVGFT
RVSPRLDFKPTIATNSELQLSQIRFK

Sequences:

>Translated_506_residues
MKLHLLAACFSTMVLALSGGTAQAQDAKSNVTVVLAETVDVVEPCMAARQDVGRVISENVNEMLVEFDYVNGGLKPRLAT
EWSKIDDDTWEFKLRPNVKWHDGKPFTAKDVQFTIERNKNKKLSCETGGKYFGGMEFSFETPDANTIRITTKPAQPILPL
LMTVMSVESAEATPADEFTRKPIGTGPYTFDKWEIGQSIVLKRNPEYWGEKPQVEQATYLFRSDSAVAAAMVDAGEADIV
PAVSVQDATNKETDFAYPNSETTSLRIDTRAAPLNDRRIREAMNLAIDRQAMLGTLFPEQAKIATQLVVPTTIGYNADIP
AWPYDPEKAKELVAAAKADGVPIDREIRIIGRNGQYPNATEAMEAMMAMLQEVGLNVKLDMYDVSVWNGYFVAPFVADSG
PTLTQSQHDNATGDPVFTAFVKYATDGSHSMVRDPAVDALIAKATSATGDERTKLWKELFAKVNTEIIADIPMFHMVGFT
RVSPRLDFKPTIATNSELQLSQIRFK
>Mature_506_residues
MKLHLLAACFSTMVLALSGGTAQAQDAKSNVTVVLAETVDVVEPCMAARQDVGRVISENVNEMLVEFDYVNGGLKPRLAT
EWSKIDDDTWEFKLRPNVKWHDGKPFTAKDVQFTIERNKNKKLSCETGGKYFGGMEFSFETPDANTIRITTKPAQPILPL
LMTVMSVESAEATPADEFTRKPIGTGPYTFDKWEIGQSIVLKRNPEYWGEKPQVEQATYLFRSDSAVAAAMVDAGEADIV
PAVSVQDATNKETDFAYPNSETTSLRIDTRAAPLNDRRIREAMNLAIDRQAMLGTLFPEQAKIATQLVVPTTIGYNADIP
AWPYDPEKAKELVAAAKADGVPIDREIRIIGRNGQYPNATEAMEAMMAMLQEVGLNVKLDMYDVSVWNGYFVAPFVADSG
PTLTQSQHDNATGDPVFTAFVKYATDGSHSMVRDPAVDALIAKATSATGDERTKLWKELFAKVNTEIIADIPMFHMVGFT
RVSPRLDFKPTIATNSELQLSQIRFK

Specific function: Important role in heme acquisition or metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=360, Percent_Identity=30.2777777777778, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1787052, Length=370, Percent_Identity=26.4864864864865, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789887, Length=498, Percent_Identity=23.0923694779116, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787762, Length=352, Percent_Identity=26.1363636363636, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1787551, Length=361, Percent_Identity=24.6537396121884, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787495, Length=419, Percent_Identity=24.3436754176611, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081878, Length=406, Percent_Identity=23.6453201970443, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082063, Length=255, Percent_Identity=25.8823529411765, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789397, Length=282, Percent_Identity=24.468085106383, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55872; Mature: 55872

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHLLAACFSTMVLALSGGTAQAQDAKSNVTVVLAETVDVVEPCMAARQDVGRVISENV
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCH
NEMLVEFDYVNGGLKPRLATEWSKIDDDTWEFKLRPNVKWHDGKPFTAKDVQFTIERNKN
HHHEEEEEECCCCCCCCHHCCHHHCCCCCEEEEECCCEEECCCCCCCCCCEEEEEECCCC
KKLSCETGGKYFGGMEFSFETPDANTIRITTKPAQPILPLLMTVMSVESAEATPADEFTR
CEEEECCCCCEECCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHC
KPIGTGPYTFDKWEIGQSIVLKRNPEYWGEKPQVEQATYLFRSDSAVAAAMVDAGEADIV
CCCCCCCCCCCHHHCCCEEEEECCCHHCCCCCCCCHHEEEEECCCCEEEEEECCCCCCCE
PAVSVQDATNKETDFAYPNSETTSLRIDTRAAPLNDRRIREAMNLAIDRQAMLGTLFPEQ
EEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AKIATQLVVPTTIGYNADIPAWPYDPEKAKELVAAAKADGVPIDREIRIIGRNGQYPNAT
HHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHH
EAMEAMMAMLQEVGLNVKLDMYDVSVWNGYFVAPFVADSGPTLTQSQHDNATGDPVFTAF
HHHHHHHHHHHHCCCCEEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
VKYATDGSHSMVRDPAVDALIAKATSATGDERTKLWKELFAKVNTEIIADIPMFHMVGFT
HHHHCCCCCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHCCE
RVSPRLDFKPTIATNSELQLSQIRFK
ECCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MKLHLLAACFSTMVLALSGGTAQAQDAKSNVTVVLAETVDVVEPCMAARQDVGRVISENV
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCH
NEMLVEFDYVNGGLKPRLATEWSKIDDDTWEFKLRPNVKWHDGKPFTAKDVQFTIERNKN
HHHEEEEEECCCCCCCCHHCCHHHCCCCCEEEEECCCEEECCCCCCCCCCEEEEEECCCC
KKLSCETGGKYFGGMEFSFETPDANTIRITTKPAQPILPLLMTVMSVESAEATPADEFTR
CEEEECCCCCEECCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHC
KPIGTGPYTFDKWEIGQSIVLKRNPEYWGEKPQVEQATYLFRSDSAVAAAMVDAGEADIV
CCCCCCCCCCCHHHCCCEEEEECCCHHCCCCCCCCHHEEEEECCCCEEEEEECCCCCCCE
PAVSVQDATNKETDFAYPNSETTSLRIDTRAAPLNDRRIREAMNLAIDRQAMLGTLFPEQ
EEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AKIATQLVVPTTIGYNADIPAWPYDPEKAKELVAAAKADGVPIDREIRIIGRNGQYPNAT
HHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHH
EAMEAMMAMLQEVGLNVKLDMYDVSVWNGYFVAPFVADSGPTLTQSQHDNATGDPVFTAF
HHHHHHHHHHHHCCCCEEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
VKYATDGSHSMVRDPAVDALIAKATSATGDERTKLWKELFAKVNTEIIADIPMFHMVGFT
HHHHCCCCCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHCCE
RVSPRLDFKPTIATNSELQLSQIRFK
ECCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339409; 7542800; 2041470 [H]