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The map label for this gene is psuG2

Identifier: 116248846

GI number: 116248846

Start: 176030

End: 176959

Strand: Direct

Name: psuG2

Synonym: pRL120175

Alternate gene names: 116248846

Gene position: 176030-176959 (Clockwise)

Preceding gene: 116248845

Following gene: 116248847

Centisome position: 20.23

GC content: 62.69

Gene sequence:

>930_bases
ATGACCCTCATGAAGCCCCGACTGAGCCGCGAAATGGCCGAAGCCATTGCAGCCGGCAGCCCGGTCGTAGCGCTTGAATC
CACCATCATCACCCATGGCATGCCTTATCCGGCCAACCTCGAAACGGCCCTGGGCGTGGAAACGGTGATCCGTGAAAACG
GCGCCATTCCGGCCACGATCGCTGTCGTCAAGGGCGAACTGCGCGTTGGCCTTGAGCACGATGAGCTGGAAGAGCTGGCG
CAGTCAAAGGGCATCGTGAAGGCCTCTGGCCGCGACCTGGCCGTCGCCATGATACGCGGTCAGTCGGCCGGCACGACGGT
TTCGGCCACCATGCTGATGGCAGACCTCGCCGGCATCGACGTCTTTGCGACCGGCGGTGTCGGCGGCGTGCATCGCGGCG
CCGAGCAGACCTTCGACATCTCCGCCGACCTGACGGAATTGGGCAGAACGAAAACCGCTGTCGTCTGCGCCGGCGTAAAA
TCGATCCTCGACATCGCCAAGACGCTCGAATATCTCGAAACTCAGCGAGTGCCGGTGATCGCCTACGGCACCGAGGATTT
CCCGGCTTTCTTCACGCGCAGGAGCGGCTTCAAGGCCGACCATCGGCTCGACACGCCGGAAGAGATCGCCAAGGCGATGT
GGTTGCACCATCAACTCGGCACGGGCACCGGCCTTTTGATCGCCAACCCGATCCCGGAAGCGTCAGCGCTGGCGCCAGAC
TTCATCGACGGCACGATTGCCGATGCGGTGCGCGATGCCGACGAGCGTGGCATCGACCGCAAGGAACTGACACCTTTCCT
GCTCGCCCGCATCAACGAATTGACGAAGGGCGAAAGCCTCAAGGCCAATATTGAACTGGTGAAGAACAATGCCCGCCTCG
CCGCCAGGATAGCGGTCGCCTATGCGCCGCTGAAGAAGGCCCGCAACTGA

Upstream 100 bases:

>100_bases
CACCGCCAGCGTCCACACCGAATTGTCCGAACGCTTCCTCGAAGCGAACATGCACCGGCTTGTCGGCTAACCTCGCCGGC
TGAATCAAGGAATTGTCACA

Downstream 100 bases:

>100_bases
CTATCAAACAGGGGAACTGCCATGAGCTACGAGACGATCCTTTACGAGAAGGATGCCAGCGACAAGTTCGCGACGATCAC
CATCAATCGCCCAGACAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: PsiMP glycosidase 2

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTLMKPRLSREMAEAIAAGSPVVALESTIITHGMPYPANLETALGVETVIRENGAIPATIAVVKGELRVGLEHDELEELA
QSKGIVKASGRDLAVAMIRGQSAGTTVSATMLMADLAGIDVFATGGVGGVHRGAEQTFDISADLTELGRTKTAVVCAGVK
SILDIAKTLEYLETQRVPVIAYGTEDFPAFFTRRSGFKADHRLDTPEEIAKAMWLHHQLGTGTGLLIANPIPEASALAPD
FIDGTIADAVRDADERGIDRKELTPFLLARINELTKGESLKANIELVKNNARLAARIAVAYAPLKKARN

Sequences:

>Translated_309_residues
MTLMKPRLSREMAEAIAAGSPVVALESTIITHGMPYPANLETALGVETVIRENGAIPATIAVVKGELRVGLEHDELEELA
QSKGIVKASGRDLAVAMIRGQSAGTTVSATMLMADLAGIDVFATGGVGGVHRGAEQTFDISADLTELGRTKTAVVCAGVK
SILDIAKTLEYLETQRVPVIAYGTEDFPAFFTRRSGFKADHRLDTPEEIAKAMWLHHQLGTGTGLLIANPIPEASALAPD
FIDGTIADAVRDADERGIDRKELTPFLLARINELTKGESLKANIELVKNNARLAARIAVAYAPLKKARN
>Mature_308_residues
TLMKPRLSREMAEAIAAGSPVVALESTIITHGMPYPANLETALGVETVIRENGAIPATIAVVKGELRVGLEHDELEELAQ
SKGIVKASGRDLAVAMIRGQSAGTTVSATMLMADLAGIDVFATGGVGGVHRGAEQTFDISADLTELGRTKTAVVCAGVKS
ILDIAKTLEYLETQRVPVIAYGTEDFPAFFTRRSGFKADHRLDTPEEIAKAMWLHHQLGTGTGLLIANPIPEASALAPDF
IDGTIADAVRDADERGIDRKELTPFLLARINELTKGESLKANIELVKNNARLAARIAVAYAPLKKARN

Specific function: Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil

COG id: COG2313

COG function: function code Q; Uncharacterized enzyme involved in pigment biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine-5'-phosphate glycosidase family

Homologues:

Organism=Escherichia coli, GI1788490, Length=299, Percent_Identity=54.180602006689, Blast_Score=308, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25147470, Length=293, Percent_Identity=52.2184300341297, Blast_Score=299, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580669, Length=299, Percent_Identity=51.1705685618729, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PSUG2_RHIL3 (Q1M4T3)

Other databases:

- EMBL:   AM236086
- RefSeq:   YP_764687.1
- ProteinModelPortal:   Q1M4T3
- SMR:   Q1M4T3
- STRING:   Q1M4T3
- GeneID:   4398464
- GenomeReviews:   AM236086_GR
- KEGG:   rle:pRL120175
- NMPDR:   fig|216596.1.peg.5881
- eggNOG:   COG2313
- HOGENOM:   HBG308892
- OMA:   NEFPAFF
- PhylomeDB:   Q1M4T3
- ProtClustDB:   CLSK862888
- BioCyc:   RLEG216596:PRL120175-MONOMER
- HAMAP:   MF_01876
- InterPro:   IPR022830
- InterPro:   IPR007342
- PANTHER:   PTHR10584:SF1

Pfam domain/function: PF04227 Indigoidine_A; SSF110581 Indigdn_synthA

EC number: NA

Molecular weight: Translated: 32889; Mature: 32758

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLMKPRLSREMAEAIAAGSPVVALESTIITHGMPYPANLETALGVETVIRENGAIPATI
CCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
AVVKGELRVGLEHDELEELAQSKGIVKASGRDLAVAMIRGQSAGTTVSATMLMADLAGID
EEEECCEEECCCHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCE
VFATGGVGGVHRGAEQTFDISADLTELGRTKTAVVCAGVKSILDIAKTLEYLETQRVPVI
EEECCCCCCCCCCCCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
AYGTEDFPAFFTRRSGFKADHRLDTPEEIAKAMWLHHQLGTGTGLLIANPIPEASALAPD
EECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCCCH
FIDGTIADAVRDADERGIDRKELTPFLLARINELTKGESLKANIELVKNNARLAARIAVA
HHCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHH
YAPLKKARN
HHHHHHCCC
>Mature Secondary Structure 
TLMKPRLSREMAEAIAAGSPVVALESTIITHGMPYPANLETALGVETVIRENGAIPATI
CCCCHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
AVVKGELRVGLEHDELEELAQSKGIVKASGRDLAVAMIRGQSAGTTVSATMLMADLAGID
EEEECCEEECCCHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCE
VFATGGVGGVHRGAEQTFDISADLTELGRTKTAVVCAGVKSILDIAKTLEYLETQRVPVI
EEECCCCCCCCCCCCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
AYGTEDFPAFFTRRSGFKADHRLDTPEEIAKAMWLHHQLGTGTGLLIANPIPEASALAPD
EECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCCCH
FIDGTIADAVRDADERGIDRKELTPFLLARINELTKGESLKANIELVKNNARLAARIAVA
HHCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHH
YAPLKKARN
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA