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The map label for this gene is fadB1 [H]

Identifier: 116248847

GI number: 116248847

Start: 176981

End: 177790

Strand: Direct

Name: fadB1 [H]

Synonym: pRL120176

Alternate gene names: 116248847

Gene position: 176981-177790 (Clockwise)

Preceding gene: 116248846

Following gene: 116248848

Centisome position: 20.34

GC content: 59.14

Gene sequence:

>810_bases
ATGAGCTACGAGACGATCCTTTACGAGAAGGATGCCAGCGACAAGTTCGCGACGATCACCATCAATCGCCCAGACAAGAT
GAATGCGATGAACAAGGTGGTGATCCGCGAGATCGATGCGGCCGTGGCTGCAGCCGTTGCAGATCCCGATATCAACGCGC
TGGTCATCACCGGCGCCGGCCGCGCCTTCTCCTCCGGTTACGATCTGCAGGGCGGCGATTTCGACGTCGATATCGATTTT
TGGCGCGAGGACATGTCGGAGAATGCCGAAGCGCTGCTTAACATCTGGCGCGCCCCGATTCCGGTCATCGCCTCGGTCAA
CGGCTATGCCCTGGCTGGCGCGCTGGAACTGATGATGTGCTGCGATCTGGCGATTGCCGCCGACAATGCCAAGTTCGGCG
AACCGGAAGTGCGCCACAATTCCGGTCCTCCGGCGCTGATGATGCCTTGGTTGCTTGCCACTCGCGACGTCCGCTGGCTG
ATGTTCACCGGCGACATGGTGGATGCGCAAGAGGCGCTGCGCATGCATCTGATCAATAAGATCGTGCCCGCCGATCAGCT
CAAGGAAAAAACCGAGAACCTGGCCCGTAAGCTGGCCCGTATGCCGCTGCCTGCGCTGAGATACACCAAGGCCTCGATCA
ATAACCAGCAGATGGTTGCCGGCCTGATGCCGTCCTTCCAGTACAATATCGAGGCGATCGCAGCCCTACACGTGACCAAG
GCGGGTCGTGACTGGATGGCCAACCTCGCAAAGATGTCGCTGAAGGAATATCTCGCTTTCCGCGACAATCCGTTCAAGGG
CCTCGACTGA

Upstream 100 bases:

>100_bases
TGAACTGGTGAAGAACAATGCCCGCCTCGCCGCCAGGATAGCGGTCGCCTATGCGCCGCTGAAGAAGGCCCGCAACTGAC
TATCAAACAGGGGAACTGCC

Downstream 100 bases:

>100_bases
GGTCCTGGCCATGCCCGCAAACACGATCGATGCCATGCGGGCTTTGATGGCGCCGCGCTCCGTCGCGGTCGTCGGTGCCA
CCGAGCGCGCCGACGCCTCG

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSYETILYEKDASDKFATITINRPDKMNAMNKVVIREIDAAVAAAVADPDINALVITGAGRAFSSGYDLQGGDFDVDIDF
WREDMSENAEALLNIWRAPIPVIASVNGYALAGALELMMCCDLAIAADNAKFGEPEVRHNSGPPALMMPWLLATRDVRWL
MFTGDMVDAQEALRMHLINKIVPADQLKEKTENLARKLARMPLPALRYTKASINNQQMVAGLMPSFQYNIEAIAALHVTK
AGRDWMANLAKMSLKEYLAFRDNPFKGLD

Sequences:

>Translated_269_residues
MSYETILYEKDASDKFATITINRPDKMNAMNKVVIREIDAAVAAAVADPDINALVITGAGRAFSSGYDLQGGDFDVDIDF
WREDMSENAEALLNIWRAPIPVIASVNGYALAGALELMMCCDLAIAADNAKFGEPEVRHNSGPPALMMPWLLATRDVRWL
MFTGDMVDAQEALRMHLINKIVPADQLKEKTENLARKLARMPLPALRYTKASINNQQMVAGLMPSFQYNIEAIAALHVTK
AGRDWMANLAKMSLKEYLAFRDNPFKGLD
>Mature_268_residues
SYETILYEKDASDKFATITINRPDKMNAMNKVVIREIDAAVAAAVADPDINALVITGAGRAFSSGYDLQGGDFDVDIDFW
REDMSENAEALLNIWRAPIPVIASVNGYALAGALELMMCCDLAIAADNAKFGEPEVRHNSGPPALMMPWLLATRDVRWLM
FTGDMVDAQEALRMHLINKIVPADQLKEKTENLARKLARMPLPALRYTKASINNQQMVAGLMPSFQYNIEAIAALHVTKA
GRDWMANLAKMSLKEYLAFRDNPFKGLD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=207, Percent_Identity=29.951690821256, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI194097323, Length=219, Percent_Identity=31.5068493150685, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI70995211, Length=198, Percent_Identity=32.8282828282828, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI68989263, Length=207, Percent_Identity=28.5024154589372, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI45643119, Length=223, Percent_Identity=27.8026905829596, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=223, Percent_Identity=27.8026905829596, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260274832, Length=223, Percent_Identity=27.8026905829596, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788597, Length=274, Percent_Identity=33.2116788321168, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787659, Length=216, Percent_Identity=31.9444444444444, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI221142681, Length=186, Percent_Identity=33.8709677419355, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI87082183, Length=202, Percent_Identity=28.7128712871287, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787660, Length=206, Percent_Identity=29.126213592233, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790281, Length=205, Percent_Identity=24.8780487804878, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=27.7227722772277, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=220, Percent_Identity=30.4545454545455, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=225, Percent_Identity=32, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=217, Percent_Identity=27.6497695852535, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=26.1904761904762, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=220, Percent_Identity=24.5454545454545, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=203, Percent_Identity=33.4975369458128, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24653477, Length=203, Percent_Identity=33.4975369458128, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21357171, Length=222, Percent_Identity=29.7297297297297, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922422, Length=179, Percent_Identity=25.1396648044693, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654903, Length=208, Percent_Identity=24.5192307692308, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=26.0223048327138, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24650670, Length=214, Percent_Identity=23.8317757009346, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29815; Mature: 29684

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYETILYEKDASDKFATITINRPDKMNAMNKVVIREIDAAVAAAVADPDINALVITGAG
CCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
RAFSSGYDLQGGDFDVDIDFWREDMSENAEALLNIWRAPIPVIASVNGYALAGALELMMC
CCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHH
CDLAIAADNAKFGEPEVRHNSGPPALMMPWLLATRDVRWLMFTGDMVDAQEALRMHLINK
HHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
IVPADQLKEKTENLARKLARMPLPALRYTKASINNQQMVAGLMPSFQYNIEAIAALHVTK
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHEEEHHHH
AGRDWMANLAKMSLKEYLAFRDNPFKGLD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SYETILYEKDASDKFATITINRPDKMNAMNKVVIREIDAAVAAAVADPDINALVITGAG
CCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
RAFSSGYDLQGGDFDVDIDFWREDMSENAEALLNIWRAPIPVIASVNGYALAGALELMMC
CCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHH
CDLAIAADNAKFGEPEVRHNSGPPALMMPWLLATRDVRWLMFTGDMVDAQEALRMHLINK
HHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
IVPADQLKEKTENLARKLARMPLPALRYTKASINNQQMVAGLMPSFQYNIEAIAALHVTK
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHEEEHHHH
AGRDWMANLAKMSLKEYLAFRDNPFKGLD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]