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The map label for this gene is pcbC [H]

Identifier: 116248844

GI number: 116248844

Start: 173811

End: 174785

Strand: Reverse

Name: pcbC [H]

Synonym: pRL120173

Alternate gene names: 116248844

Gene position: 174785-173811 (Counterclockwise)

Preceding gene: 116248849

Following gene: 116248843

Centisome position: 20.09

GC content: 61.85

Gene sequence:

>975_bases
ATGTCCGTATCGAACGCCAATACCGCGTCGTCCAACGGTGTCGGGGCCGGCATTCCCGTCATCGATGTGGGGCCTTTCAT
CCGTAATGAGGTGGGCAGCGCCGATGTGGTGAAGGCGATCGAGGCCGCCTGTCGCGACACCGGCTTCTTCCTTGTCACCG
GCCATGGTGTTTCGCTGGACGTCACCTCGCGGCTCTATCAACTCGCCCGCGCCTTTTTCGATTACCCGCAAGACTGGAAG
ACGACGCAAGGGCGCGGCACGGGCGTGATGGGCGGCGTCGCCTTCTCGCCCCTCGCCGAGGAAGCATTGGCCGCGACGCT
CGGCATCAAGACGCCCGGCGACTACAAGGAAAGCCTGAACTTCGGTCAGAACCTGCCGGGCGATACGTGGCCCAACAGGC
CGGAAGGGATCGAGCGGGCCTTCCGCGACTATTTCAAGGAAATGGAAATCCTGGCCAAGCACATGCGCCGGATCTTCTGC
CAGGCGATCGGGCTTGACCAGGATTATTTCGAGCCGTCCTTCGAGAACCACCTCTCGGCACTGCGCGTCATCAACTATCC
CGAACAGGACGAAGCACCGCTGCCGGGCCAGATGCGCGCCGGCGTCCACACCGATTACGGCTTCATGACCATCTTGCGCT
CGGAAGCGTCGGCAGGCGGGTTGCAAGTGCGCAACCGCAATGGCGACTGGCTTGACGCGCCATCGATCGAGGGTGCCTAT
GTCATCAACATCGCCGATGCGTTTATGCGCTGGAGCAACGACGAGTGGGTCTCGACCCCGCATCGGGTCGCCAATCCGCC
GAGCGGCTTCAAGGGCACGGCGCGCCGCCAGTCGATCCCGTTCTTCCTCAACCCGTCGAAGGACACGGTGATCGAGTGCC
TGCCGCCTTTCTTAAAGGGCGGCGCGAAATACGAGCCGATTACCTACGGTGAATATATCGACCTGAAGACGAAACAGGCT
TTCTCGAAAGGCTGA

Upstream 100 bases:

>100_bases
TTGTCTAGTTCACTGGGTTGAAGTTTCGTTATTTTTGATATGGACAACAACAGATGTTTTTGTCAACATCTGTTTGTCAG
TCGAGTTTGGAGGTAGGCTT

Downstream 100 bases:

>100_bases
TGAGTGACTGAAGTCGACTGACTCGTGCGCAGAAGCGGAAAACCGCCGGCACAACGATCAAGGATCGCAACTAAGGGGAA
CTGGAAAATGATGGATTTGA

Product: putative Fe-containing oxidoreductase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSVSNANTASSNGVGAGIPVIDVGPFIRNEVGSADVVKAIEAACRDTGFFLVTGHGVSLDVTSRLYQLARAFFDYPQDWK
TTQGRGTGVMGGVAFSPLAEEALAATLGIKTPGDYKESLNFGQNLPGDTWPNRPEGIERAFRDYFKEMEILAKHMRRIFC
QAIGLDQDYFEPSFENHLSALRVINYPEQDEAPLPGQMRAGVHTDYGFMTILRSEASAGGLQVRNRNGDWLDAPSIEGAY
VINIADAFMRWSNDEWVSTPHRVANPPSGFKGTARRQSIPFFLNPSKDTVIECLPPFLKGGAKYEPITYGEYIDLKTKQA
FSKG

Sequences:

>Translated_324_residues
MSVSNANTASSNGVGAGIPVIDVGPFIRNEVGSADVVKAIEAACRDTGFFLVTGHGVSLDVTSRLYQLARAFFDYPQDWK
TTQGRGTGVMGGVAFSPLAEEALAATLGIKTPGDYKESLNFGQNLPGDTWPNRPEGIERAFRDYFKEMEILAKHMRRIFC
QAIGLDQDYFEPSFENHLSALRVINYPEQDEAPLPGQMRAGVHTDYGFMTILRSEASAGGLQVRNRNGDWLDAPSIEGAY
VINIADAFMRWSNDEWVSTPHRVANPPSGFKGTARRQSIPFFLNPSKDTVIECLPPFLKGGAKYEPITYGEYIDLKTKQA
FSKG
>Mature_323_residues
SVSNANTASSNGVGAGIPVIDVGPFIRNEVGSADVVKAIEAACRDTGFFLVTGHGVSLDVTSRLYQLARAFFDYPQDWKT
TQGRGTGVMGGVAFSPLAEEALAATLGIKTPGDYKESLNFGQNLPGDTWPNRPEGIERAFRDYFKEMEILAKHMRRIFCQ
AIGLDQDYFEPSFENHLSALRVINYPEQDEAPLPGQMRAGVHTDYGFMTILRSEASAGGLQVRNRNGDWLDAPSIEGAYV
INIADAFMRWSNDEWVSTPHRVANPPSGFKGTARRQSIPFFLNPSKDTVIECLPPFLKGGAKYEPITYGEYIDLKTKQAF
SKG

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=317, Percent_Identity=25.2365930599369, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 35603; Mature: 35472

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSNANTASSNGVGAGIPVIDVGPFIRNEVGSADVVKAIEAACRDTGFFLVTGHGVSLD
CCCCCCCCCCCCCCCCCCCCEECCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEH
VTSRLYQLARAFFDYPQDWKTTQGRGTGVMGGVAFSPLAEEALAATLGIKTPGDYKESLN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
FGQNLPGDTWPNRPEGIERAFRDYFKEMEILAKHMRRIFCQAIGLDQDYFEPSFENHLSA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LRVINYPEQDEAPLPGQMRAGVHTDYGFMTILRSEASAGGLQVRNRNGDWLDAPSIEGAY
HHHCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCEE
VINIADAFMRWSNDEWVSTPHRVANPPSGFKGTARRQSIPFFLNPSKDTVIECLPPFLKG
EEEEHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCHHCCCCEEECCCHHHHHHHCCHHHCC
GAKYEPITYGEYIDLKTKQAFSKG
CCCCCCCCCCCEECCHHHHHHCCC
>Mature Secondary Structure 
SVSNANTASSNGVGAGIPVIDVGPFIRNEVGSADVVKAIEAACRDTGFFLVTGHGVSLD
CCCCCCCCCCCCCCCCCCCEECCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEH
VTSRLYQLARAFFDYPQDWKTTQGRGTGVMGGVAFSPLAEEALAATLGIKTPGDYKESLN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
FGQNLPGDTWPNRPEGIERAFRDYFKEMEILAKHMRRIFCQAIGLDQDYFEPSFENHLSA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LRVINYPEQDEAPLPGQMRAGVHTDYGFMTILRSEASAGGLQVRNRNGDWLDAPSIEGAY
HHHCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCEE
VINIADAFMRWSNDEWVSTPHRVANPPSGFKGTARRQSIPFFLNPSKDTVIECLPPFLKG
EEEEHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCHHCCCCEEECCCHHHHHHHCCHHHCC
GAKYEPITYGEYIDLKTKQAFSKG
CCCCCCCCCCCEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1956290 [H]