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The map label for this gene is rihA [H]

Identifier: 116248849

GI number: 116248849

Start: 179884

End: 180882

Strand: Reverse

Name: rihA [H]

Synonym: pRL120178

Alternate gene names: 116248849

Gene position: 180882-179884 (Counterclockwise)

Preceding gene: 116248850

Following gene: 116248844

Centisome position: 20.79

GC content: 61.76

Gene sequence:

>999_bases
ATGGCACAAAAAATCATCATCGATACGGATCCGGGGCAAGACGATGCGCTGGCCATACTCCTGGCTCTGGCGAGCCCGGA
AATCGAAGTTCTCGGCATCGTTGGCGCTGCCGGCAATGTGCCGCTATTGCTGACGTCCAAAAATATCCGCAAGATCTGCG
AACTCGCCGGCCGTCCAGACATCAAGGTGTTTGCCGGCTGCGATCGGCCGATGACCGGACCGCTAGTGACCGCCGAGCAC
GTGCATGGCGCGACCGGCATGGATGGCGCGGATCTTCCTGAACCGAGCATGCCGATGCAGGAACAGCACGGCGTCGATTT
CATCATCGACACCCTGCAGAGCGAACCGGCCGGCGCAGTCACCGTCTGCACGCTTGGGCCACTTACCAATCTCGGCAAGG
CGCTGACCCGGGCGCCGGAGATCGCCGGCCGGATCAAACAGCTCGTGCTGATGGGCGGCGGACTGTTCGAGGGCGGCAAC
ATTACGCCCGCCGCCGAATTCAATATCTACGTCGATCCGCCGGCTGCAGCTGTCGTCTTCGGATCGGGCGTGCCGATCGT
GATGATGCCGCTCGACGTCACGCACAAGACGCTGACGACACGGGTACGTGTCGCTGCAATCCAGGCGCTTGGAACTCCGC
TCAGCGATGCCGTGGTCGGATGGCTTCATTATTTCGAACGCTTCGACGTCGCTAAATATGGAAGCGATGGTGGGCCGCTG
CACGATCCCAACGTGATTGCCTATCTCCTGAAGCCGGAACTTTATTCCGGTCGTTTCTGCAATGTGGAGATCGAGACGGA
GTCCGAGCTCACCAAGGGCATGACCGTCGCCGACTGGTGGGATGTCTCGGGGCGGCCGAAAAATGCGCTGTTCATGCGCG
ACGTCGACGCCGACGGCTTTTATGCGCTGCTGACGGAACGGCTCTCCTGGCTCGGTCGCGCGGATACGGCAGCCGAAGCT
GCGCCGGGTCTGCTCGTCACGTCTTCGCGCGCGCGATGA

Upstream 100 bases:

>100_bases
AGCGCTTCGAAAAGCGCAAGAACGATCTCCTGGCGAAATGGCGGGCAAGCGAACCGCTCACCCATGTCGCCTAAATTTTG
TTTCCGAGAGTGAGCCCCAA

Downstream 100 bases:

>100_bases
TCAGCGCGTCGAGAATGCGCACGCCCTCGCCATCCGGCAGAACGGCGACCGGATTGAGGTCGACAGCCTCGATATCGTCG
CCATAACGGGCGGCGAAATC

Product: putative nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAQKIIIDTDPGQDDALAILLALASPEIEVLGIVGAAGNVPLLLTSKNIRKICELAGRPDIKVFAGCDRPMTGPLVTAEH
VHGATGMDGADLPEPSMPMQEQHGVDFIIDTLQSEPAGAVTVCTLGPLTNLGKALTRAPEIAGRIKQLVLMGGGLFEGGN
ITPAAEFNIYVDPPAAAVVFGSGVPIVMMPLDVTHKTLTTRVRVAAIQALGTPLSDAVVGWLHYFERFDVAKYGSDGGPL
HDPNVIAYLLKPELYSGRFCNVEIETESELTKGMTVADWWDVSGRPKNALFMRDVDADGFYALLTERLSWLGRADTAAEA
APGLLVTSSRAR

Sequences:

>Translated_332_residues
MAQKIIIDTDPGQDDALAILLALASPEIEVLGIVGAAGNVPLLLTSKNIRKICELAGRPDIKVFAGCDRPMTGPLVTAEH
VHGATGMDGADLPEPSMPMQEQHGVDFIIDTLQSEPAGAVTVCTLGPLTNLGKALTRAPEIAGRIKQLVLMGGGLFEGGN
ITPAAEFNIYVDPPAAAVVFGSGVPIVMMPLDVTHKTLTTRVRVAAIQALGTPLSDAVVGWLHYFERFDVAKYGSDGGPL
HDPNVIAYLLKPELYSGRFCNVEIETESELTKGMTVADWWDVSGRPKNALFMRDVDADGFYALLTERLSWLGRADTAAEA
APGLLVTSSRAR
>Mature_331_residues
AQKIIIDTDPGQDDALAILLALASPEIEVLGIVGAAGNVPLLLTSKNIRKICELAGRPDIKVFAGCDRPMTGPLVTAEHV
HGATGMDGADLPEPSMPMQEQHGVDFIIDTLQSEPAGAVTVCTLGPLTNLGKALTRAPEIAGRIKQLVLMGGGLFEGGNI
TPAAEFNIYVDPPAAAVVFGSGVPIVMMPLDVTHKTLTTRVRVAAIQALGTPLSDAVVGWLHYFERFDVAKYGSDGGPLH
DPNVIAYLLKPELYSGRFCNVEIETESELTKGMTVADWWDVSGRPKNALFMRDVDADGFYALLTERLSWLGRADTAAEAA
PGLLVTSSRAR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=311, Percent_Identity=45.016077170418, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=38.1578947368421, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786213, Length=311, Percent_Identity=36.0128617363344, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32565474, Length=199, Percent_Identity=32.6633165829146, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565698, Length=294, Percent_Identity=28.2312925170068, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320608, Length=257, Percent_Identity=30.7392996108949, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24583915, Length=205, Percent_Identity=27.8048780487805, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24641837, Length=198, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24641839, Length=204, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 35254; Mature: 35123

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKIIIDTDPGQDDALAILLALASPEIEVLGIVGAAGNVPLLLTSKNIRKICELAGRPD
CCCEEEEECCCCCCHHHEEEEECCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHCCCCC
IKVFAGCDRPMTGPLVTAEHVHGATGMDGADLPEPSMPMQEQHGVDFIIDTLQSEPAGAV
EEEEECCCCCCCCCEEEHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCEE
TVCTLGPLTNLGKALTRAPEIAGRIKQLVLMGGGLFEGGNITPAAEFNIYVDPPAAAVVF
EEEECCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCEEEEE
GSGVPIVMMPLDVTHKTLTTRVRVAAIQALGTPLSDAVVGWLHYFERFDVAKYGSDGGPL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
HDPNVIAYLLKPELYSGRFCNVEIETESELTKGMTVADWWDVSGRPKNALFMRDVDADGF
CCCCEEEEEECCCCCCCEEEEEEECCHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCH
YALLTERLSWLGRADTAAEAAPGLLVTSSRAR
HHHHHHHHHHHCCCCCHHHHCCCEEEECCCCC
>Mature Secondary Structure 
AQKIIIDTDPGQDDALAILLALASPEIEVLGIVGAAGNVPLLLTSKNIRKICELAGRPD
CCEEEEECCCCCCHHHEEEEECCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHCCCCC
IKVFAGCDRPMTGPLVTAEHVHGATGMDGADLPEPSMPMQEQHGVDFIIDTLQSEPAGAV
EEEEECCCCCCCCCEEEHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCEE
TVCTLGPLTNLGKALTRAPEIAGRIKQLVLMGGGLFEGGNITPAAEFNIYVDPPAAAVVF
EEEECCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCEEEEE
GSGVPIVMMPLDVTHKTLTTRVRVAAIQALGTPLSDAVVGWLHYFERFDVAKYGSDGGPL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
HDPNVIAYLLKPELYSGRFCNVEIETESELTKGMTVADWWDVSGRPKNALFMRDVDADGF
CCCCEEEEEECCCCCCCEEEEEEECCHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCH
YALLTERLSWLGRADTAAEAAPGLLVTSSRAR
HHHHHHHHHHHCCCCCHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA