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The map label for this gene is potD [H]

Identifier: 116248843

GI number: 116248843

Start: 172686

End: 173786

Strand: Reverse

Name: potD [H]

Synonym: pRL120172

Alternate gene names: 116248843

Gene position: 173786-172686 (Counterclockwise)

Preceding gene: 116248844

Following gene: 116248842

Centisome position: 19.97

GC content: 58.86

Gene sequence:

>1101_bases
GTGCGCAGAAGCGGAAAACCGCCGGCACAACGATCAAGGATCGCAACTAAGGGGAACTGGAAAATGATGGATTTGACAAG
GCGGCAGACGCTTGCCGGAATGGGAGCATTCGCTGCCGCCGGTCTCATGGGACTTCCGGCCCGTGCACAATCTAAGACGC
TGATCGTGCCGACGCTGGGCGGGGTATGGGAACAGTACTGGCGCTCGACAGTGGCGCCGGAATTCACCAAGCAGAGCGGG
GCAGCCGTGACGCTGGACGTTGGAAACGGCCGCGTGTTCGGGGCGAATCTGCGGGCCGCCGGCGTTGCCAAGCCGCCCTA
TTCGATCGTGATGACCAACGAGGTCTTCGCCTCGGGGCTGCGCAAGGAAGGTTTCTTCGAGAAGCTCGACCTGACGAAGC
TGTCGAACTATGCCGACCTTTATCCGCTGGCCAAGAAGACCGACGGCTGGGGCGCGATCGGCGCCGTTTCCCCGATCGGC
ATCGGCTACCGCACGGACCTGGTGAAGACACCGCCAAAAAGTTGGAAGGATCTTTGGACCAACCCGGAATTCAAAGGCAA
GATCGGCCTCTACAATTTTGCCAACAGCGCCGGCAAGATGGAGTTGCTGCTGTTTTCGAGGATTTTTGGCAAGGACCAGT
ATGATGTCGACGCCGGCTTTGCCGCGCTCGAAAAGCTTGGCTCGGTCATCCAGGTGGACTTCAACCTTTCCACCGGTCTC
GCGTCAGGCGAAATCGTCGTCGCACCGTTCGATTTCGGCGAAATTGCCCGCCTGAAGGCTCAGGGACTGCCGGTCGATTG
CATCGTCCCGGAAGAAGGGATGTTCATGTTCGACCAAACCATCAACATCCTTGCCAATGGCCCGGAGAAGGAACTGGCCT
ACCAGTATGCCAACTTCCTGCTGTCGCCCGACGTGCAGACGATGATGATGAGACAGTTCTTCATCTCGCCGACCAATTCC
AAGGTCATGGTGCCGGCTGATCTCAAAGCCAGCGTGCCGGTCTCCGGCGCCGACATGGACAAGATCCTCACCTGGGACTG
GAACTTCGTCAACGAAAAGCAAGGCGAGCTCGCCGAACGCTGGGCGAAGACCATAAAGTGA

Upstream 100 bases:

>100_bases
CGGCGCGAAATACGAGCCGATTACCTACGGTGAATATATCGACCTGAAGACGAAACAGGCTTTCTCGAAAGGCTGATGAG
TGACTGAAGTCGACTGACTC

Downstream 100 bases:

>100_bases
TTTCCCCCGATGCGATCATGGCCCACCCCTGTGGCGGAGCCATGATCGGTTTTTTTTGCGGAGGATCTTTGGACCGTTGG
CTCCAGGCAATTGGAAAGTG

Product: putative substrate-binding component of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRRSGKPPAQRSRIATKGNWKMMDLTRRQTLAGMGAFAAAGLMGLPARAQSKTLIVPTLGGVWEQYWRSTVAPEFTKQSG
AAVTLDVGNGRVFGANLRAAGVAKPPYSIVMTNEVFASGLRKEGFFEKLDLTKLSNYADLYPLAKKTDGWGAIGAVSPIG
IGYRTDLVKTPPKSWKDLWTNPEFKGKIGLYNFANSAGKMELLLFSRIFGKDQYDVDAGFAALEKLGSVIQVDFNLSTGL
ASGEIVVAPFDFGEIARLKAQGLPVDCIVPEEGMFMFDQTINILANGPEKELAYQYANFLLSPDVQTMMMRQFFISPTNS
KVMVPADLKASVPVSGADMDKILTWDWNFVNEKQGELAERWAKTIK

Sequences:

>Translated_366_residues
MRRSGKPPAQRSRIATKGNWKMMDLTRRQTLAGMGAFAAAGLMGLPARAQSKTLIVPTLGGVWEQYWRSTVAPEFTKQSG
AAVTLDVGNGRVFGANLRAAGVAKPPYSIVMTNEVFASGLRKEGFFEKLDLTKLSNYADLYPLAKKTDGWGAIGAVSPIG
IGYRTDLVKTPPKSWKDLWTNPEFKGKIGLYNFANSAGKMELLLFSRIFGKDQYDVDAGFAALEKLGSVIQVDFNLSTGL
ASGEIVVAPFDFGEIARLKAQGLPVDCIVPEEGMFMFDQTINILANGPEKELAYQYANFLLSPDVQTMMMRQFFISPTNS
KVMVPADLKASVPVSGADMDKILTWDWNFVNEKQGELAERWAKTIK
>Mature_366_residues
MRRSGKPPAQRSRIATKGNWKMMDLTRRQTLAGMGAFAAAGLMGLPARAQSKTLIVPTLGGVWEQYWRSTVAPEFTKQSG
AAVTLDVGNGRVFGANLRAAGVAKPPYSIVMTNEVFASGLRKEGFFEKLDLTKLSNYADLYPLAKKTDGWGAIGAVSPIG
IGYRTDLVKTPPKSWKDLWTNPEFKGKIGLYNFANSAGKMELLLFSRIFGKDQYDVDAGFAALEKLGSVIQVDFNLSTGL
ASGEIVVAPFDFGEIARLKAQGLPVDCIVPEEGMFMFDQTINILANGPEKELAYQYANFLLSPDVQTMMMRQFFISPTNS
KVMVPADLKASVPVSGADMDKILTWDWNFVNEKQGELAERWAKTIK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=265, Percent_Identity=29.0566037735849, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40149; Mature: 40149

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSGKPPAQRSRIATKGNWKMMDLTRRQTLAGMGAFAAAGLMGLPARAQSKTLIVPTLG
CCCCCCCCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCH
GVWEQYWRSTVAPEFTKQSGAAVTLDVGNGRVFGANLRAAGVAKPPYSIVMTNEVFASGL
HHHHHHHHHHCCCCHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHCH
RKEGFFEKLDLTKLSNYADLYPLAKKTDGWGAIGAVSPIGIGYRTDLVKTPPKSWKDLWT
HCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
NPEFKGKIGLYNFANSAGKMELLLFSRIFGKDQYDVDAGFAALEKLGSVIQVDFNLSTGL
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCCC
ASGEIVVAPFDFGEIARLKAQGLPVDCIVPEEGMFMFDQTINILANGPEKELAYQYANFL
CCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCEEEECHHHEEECCCHHHHHHHHHHHH
LSPDVQTMMMRQFFISPTNSKVMVPADLKASVPVSGADMDKILTWDWNFVNEKQGELAER
CCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHH
WAKTIK
HHHHCC
>Mature Secondary Structure
MRRSGKPPAQRSRIATKGNWKMMDLTRRQTLAGMGAFAAAGLMGLPARAQSKTLIVPTLG
CCCCCCCCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCH
GVWEQYWRSTVAPEFTKQSGAAVTLDVGNGRVFGANLRAAGVAKPPYSIVMTNEVFASGL
HHHHHHHHHHCCCCHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHCH
RKEGFFEKLDLTKLSNYADLYPLAKKTDGWGAIGAVSPIGIGYRTDLVKTPPKSWKDLWT
HCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
NPEFKGKIGLYNFANSAGKMELLLFSRIFGKDQYDVDAGFAALEKLGSVIQVDFNLSTGL
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCCC
ASGEIVVAPFDFGEIARLKAQGLPVDCIVPEEGMFMFDQTINILANGPEKELAYQYANFL
CCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCEEEECHHHEEECCCHHHHHHHHHHHH
LSPDVQTMMMRQFFISPTNSKVMVPADLKASVPVSGADMDKILTWDWNFVNEKQGELAER
CCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHH
WAKTIK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]