Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 116248836

GI number: 116248836

Start: 163914

End: 165497

Strand: Reverse

Name: gsiB [H]

Synonym: pRL120164

Alternate gene names: 116248836

Gene position: 165497-163914 (Counterclockwise)

Preceding gene: 116248837

Following gene: 116248835

Centisome position: 19.02

GC content: 60.16

Gene sequence:

>1584_bases
ATGAATGACATCAGCAGACGCAATTTCCTCGCCGGGCTTGGTGCAGCTGGCACGGGTGCCGGTCTCCTGCAGATCAACCC
GGATTTTCTGTTCTCCTCGGCCTTTGCCCAATCGGGCAAGACGGTGGTGTTCCTGTCGGCGGAAAATATTACGGGCAACT
GGGACCCGACCGGCCACACGACGCTGTCGCAGAAGAATATCGAAGGCTTCGTCATGGGCTTCCTGACGCGGACGCCGATG
ACTGTCGAAAAGCCCGATGAAGTGGTCTATGAACTGGCCACCAGCATCAAGCTTCTCGATCCGAACAAGCTCGAGATCAA
GCTGCGCGAAGGCGTCAAATTCCACGACGGCAAACCCTTCAAGGCCGAAGACGTCAAGGCGACCTTCGAATACGGCTCCG
GGCCGGACCGCCCGGCCCAATTCTATCCCGGCCCGACCGAGACGCTTGTTATCACCACCCCTGACGACTACACGGTCATC
GTCGATACCTCTAAGGGCGGATATCCGGCCCATCTGTTCATCTTCCTCGCCTCATACCTGCCGATCATGTCGGCGACCGA
TGTCGCCGGCGGCCCGACCGGCCCGCTGACGCAGCGCCTGAACGGCACCGGCCCGTTCAAGTTCGTCAAGCAGGAAGGCA
ATGCCACGATCCTCGAAGCCTATCCTGATTATTTCCTCGGTACACCGAAGATTCCTGGCATCAGCTTCAATTTCGTCGGC
GACAGCACGACGCGCATGTTGTCCCTGCTCAACGGCCAGGCCTCGATCGTCGAGCGTCTGGAACCGGAACAGGTGACTTC
GCTCCAGGGCAACGACAAGATCGCCATCAGCAGCGTCGTTTCGGTCGAGAACAAGTATCTCTGGTTCCGCTGCTCGAAGG
CGCCGTTCGACAACCCGCTGATCCGCAAGGCGGCCTGCCACGCGATCGACCGCTCGATGATCGTCGAAATTCTGGGCTCG
GCCGGTCATGCTTCGTCCAATTACGTGTCGCCGGTGAAGTTCGGCTATATCGACCTGCCGAATTATCCAGAATACAGCCC
GGAAAAATGCCAGGCGCTGCTGGCCGAGGCCGGCTTCCCGAAGGGCGAGGGCCTGCCGCCGCTGGAATACATCACCTCCG
TCGGCTTCTACCCGAAGACCAAGGAATACGGCGAGGTCATCACCGCCATGCTGCAGGAACAGGGTTTTCAGGTGTCGCTC
TCCGTTCTGGAAATCGCCGCCTGGAACGAACGCCTCTACGACCGTCCGGGCGGCGGCCCCGGCCATATGGTCGATTGCGG
CTGGTCCACCGGTTCGCCCGAGCCCGACCTCGTGCTGCGCACGCATTTCCATTCGACCTCGAAGCGCATTACCGGCATCG
TCGACAAGGAACTCGACGCGACACTCGACAAGGAGCGCAGTGCCCCGTCGCTCGATGAGCGCAAGAAAATCCTGCAGACC
GAAACCATGCCCATGCTTGCCGAAAAGGTGCCGGCCCTGTCACTGTTCACTTCGGTGATGATCCACGCGATGGACAAGTC
GCTGGCCGGTCTCTTCATATATCCTGACGGATCCATGGACGCCTCGAAGGCCGCATTCAGCTGA

Upstream 100 bases:

>100_bases
GGCTTTTCTAAATTACGAGAGCCCATTGAAAAAGGAAAAAATGCCCGATATCGTGGAATAATAAGAAAGCCACATAAAAC
CACAAAAAGGGAAGCAGCTT

Downstream 100 bases:

>100_bases
TGCTAGCTTGCGTTTTCCGCGGCGCTGGAGGCCGGCGCCGCGGAGGTTTTCGTTTCGCTCATGATGTCAACAAACGACCG
CGACCGTCACCGCTCAGGAC

Product: putative component of ABC transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MNDISRRNFLAGLGAAGTGAGLLQINPDFLFSSAFAQSGKTVVFLSAENITGNWDPTGHTTLSQKNIEGFVMGFLTRTPM
TVEKPDEVVYELATSIKLLDPNKLEIKLREGVKFHDGKPFKAEDVKATFEYGSGPDRPAQFYPGPTETLVITTPDDYTVI
VDTSKGGYPAHLFIFLASYLPIMSATDVAGGPTGPLTQRLNGTGPFKFVKQEGNATILEAYPDYFLGTPKIPGISFNFVG
DSTTRMLSLLNGQASIVERLEPEQVTSLQGNDKIAISSVVSVENKYLWFRCSKAPFDNPLIRKAACHAIDRSMIVEILGS
AGHASSNYVSPVKFGYIDLPNYPEYSPEKCQALLAEAGFPKGEGLPPLEYITSVGFYPKTKEYGEVITAMLQEQGFQVSL
SVLEIAAWNERLYDRPGGGPGHMVDCGWSTGSPEPDLVLRTHFHSTSKRITGIVDKELDATLDKERSAPSLDERKKILQT
ETMPMLAEKVPALSLFTSVMIHAMDKSLAGLFIYPDGSMDASKAAFS

Sequences:

>Translated_527_residues
MNDISRRNFLAGLGAAGTGAGLLQINPDFLFSSAFAQSGKTVVFLSAENITGNWDPTGHTTLSQKNIEGFVMGFLTRTPM
TVEKPDEVVYELATSIKLLDPNKLEIKLREGVKFHDGKPFKAEDVKATFEYGSGPDRPAQFYPGPTETLVITTPDDYTVI
VDTSKGGYPAHLFIFLASYLPIMSATDVAGGPTGPLTQRLNGTGPFKFVKQEGNATILEAYPDYFLGTPKIPGISFNFVG
DSTTRMLSLLNGQASIVERLEPEQVTSLQGNDKIAISSVVSVENKYLWFRCSKAPFDNPLIRKAACHAIDRSMIVEILGS
AGHASSNYVSPVKFGYIDLPNYPEYSPEKCQALLAEAGFPKGEGLPPLEYITSVGFYPKTKEYGEVITAMLQEQGFQVSL
SVLEIAAWNERLYDRPGGGPGHMVDCGWSTGSPEPDLVLRTHFHSTSKRITGIVDKELDATLDKERSAPSLDERKKILQT
ETMPMLAEKVPALSLFTSVMIHAMDKSLAGLFIYPDGSMDASKAAFS
>Mature_527_residues
MNDISRRNFLAGLGAAGTGAGLLQINPDFLFSSAFAQSGKTVVFLSAENITGNWDPTGHTTLSQKNIEGFVMGFLTRTPM
TVEKPDEVVYELATSIKLLDPNKLEIKLREGVKFHDGKPFKAEDVKATFEYGSGPDRPAQFYPGPTETLVITTPDDYTVI
VDTSKGGYPAHLFIFLASYLPIMSATDVAGGPTGPLTQRLNGTGPFKFVKQEGNATILEAYPDYFLGTPKIPGISFNFVG
DSTTRMLSLLNGQASIVERLEPEQVTSLQGNDKIAISSVVSVENKYLWFRCSKAPFDNPLIRKAACHAIDRSMIVEILGS
AGHASSNYVSPVKFGYIDLPNYPEYSPEKCQALLAEAGFPKGEGLPPLEYITSVGFYPKTKEYGEVITAMLQEQGFQVSL
SVLEIAAWNERLYDRPGGGPGHMVDCGWSTGSPEPDLVLRTHFHSTSKRITGIVDKELDATLDKERSAPSLDERKKILQT
ETMPMLAEKVPALSLFTSVMIHAMDKSLAGLFIYPDGSMDASKAAFS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=533, Percent_Identity=26.078799249531, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789966, Length=437, Percent_Identity=24.0274599542334, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787762, Length=451, Percent_Identity=25.7206208425721, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787551, Length=460, Percent_Identity=23.2608695652174, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57388; Mature: 57388

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDISRRNFLAGLGAAGTGAGLLQINPDFLFSSAFAQSGKTVVFLSAENITGNWDPTGHT
CCCCHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCC
TLSQKNIEGFVMGFLTRTPMTVEKPDEVVYELATSIKLLDPNKLEIKLREGVKFHDGKPF
EECCCCCCHHEEHHHHCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEEECCCEECCCCCC
KAEDVKATFEYGSGPDRPAQFYPGPTETLVITTPDDYTVIVDTSKGGYPAHLFIFLASYL
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
PIMSATDVAGGPTGPLTQRLNGTGPFKFVKQEGNATILEAYPDYFLGTPKIPGISFNFVG
HHHCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCEEEEECCCCHHCCCCCCCCEEEEECC
DSTTRMLSLLNGQASIVERLEPEQVTSLQGNDKIAISSVVSVENKYLWFRCSKAPFDNPL
CCHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCCCCCHH
IRKAACHAIDRSMIVEILGSAGHASSNYVSPVKFGYIDLPNYPEYSPEKCQALLAEAGFP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCC
KGEGLPPLEYITSVGFYPKTKEYGEVITAMLQEQGFQVSLSVLEIAAWNERLYDRPGGGP
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHCCCCCCC
GHMVDCGWSTGSPEPDLVLRTHFHSTSKRITGIVDKELDATLDKERSAPSLDERKKILQT
CCEEECCCCCCCCCCCEEEEEECCCCCHHHEEECCCHHHHHHHHCCCCCCHHHHHHHHHH
ETMPMLAEKVPALSLFTSVMIHAMDKSLAGLFIYPDGSMDASKAAFS
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC
>Mature Secondary Structure
MNDISRRNFLAGLGAAGTGAGLLQINPDFLFSSAFAQSGKTVVFLSAENITGNWDPTGHT
CCCCHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCC
TLSQKNIEGFVMGFLTRTPMTVEKPDEVVYELATSIKLLDPNKLEIKLREGVKFHDGKPF
EECCCCCCHHEEHHHHCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEEECCCEECCCCCC
KAEDVKATFEYGSGPDRPAQFYPGPTETLVITTPDDYTVIVDTSKGGYPAHLFIFLASYL
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
PIMSATDVAGGPTGPLTQRLNGTGPFKFVKQEGNATILEAYPDYFLGTPKIPGISFNFVG
HHHCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCEEEEECCCCHHCCCCCCCCEEEEECC
DSTTRMLSLLNGQASIVERLEPEQVTSLQGNDKIAISSVVSVENKYLWFRCSKAPFDNPL
CCHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCCCCCHH
IRKAACHAIDRSMIVEILGSAGHASSNYVSPVKFGYIDLPNYPEYSPEKCQALLAEAGFP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCC
KGEGLPPLEYITSVGFYPKTKEYGEVITAMLQEQGFQVSLSVLEIAAWNERLYDRPGGGP
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHCCCCCCC
GHMVDCGWSTGSPEPDLVLRTHFHSTSKRITGIVDKELDATLDKERSAPSLDERKKILQT
CCEEECCCCCCCCCCCEEEEEECCCCCHHHEEECCCHHHHHHHHCCCCCCHHHHHHHHHH
ETMPMLAEKVPALSLFTSVMIHAMDKSLAGLFIYPDGSMDASKAAFS
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA