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The map label for this gene is lip2 [H]

Identifier: 116248699

GI number: 116248699

Start: 21620

End: 22555

Strand: Reverse

Name: lip2 [H]

Synonym: pRL120023

Alternate gene names: 116248699

Gene position: 22555-21620 (Counterclockwise)

Preceding gene: 116248700

Following gene: 116248697

Centisome position: 2.59

GC content: 64.53

Gene sequence:

>936_bases
ATGACACAACTGGATGAATCCGCCAAACGCGCGCTTGCATGGCCTGGCGATCCGCAAACGCCGGTCGAAAGCTGCACTCC
GGCACTGGCGCGGCAGCAATATCTGGATAGTTTCGCAGACATTCAACGGCCGCTCGAAGACGTGGTCGAGACCCTAGAAA
GGCATGCAGGCGCCATCCGGTTGAAGATATGGCGCGGCCGCGCTGCGCCGCGCCAGGGCGCGCCGGCCTTGCTTTATCTC
CATGGCGGCGGTTTTGTGATCGGCGCGCCGGAAACCCATGAGGATATCTGCCGGACGCTTGCCAATATGGCAGGCGCCGT
CGTGGTTTCGCCCGACTATCGCCTGGCCCCGGAACATCCCTTTCCTGCTGCCATTGACGACTGCGCCGCCGCACTCGTCT
GGATGACGGAGCAGGCTGATGCGCTCGGCATCGATCCCTTGCGGGTTGTCGTCGCCGGCGATAGCGCCGGCGGCAATCTG
GCCGCCGTCGTCGCCCTGCTGGCGCGGGACGGACAGGTGCCGGCTGTCATCGGACAGGTGCTGATCTATCCGGTGACCGA
CCAGTTGCAGACAAGCGACAGCTACTCACGTTACGAAGAGGATTTCGGCCTGACGGCAGCCGCGATGAAATGGTTCCGAG
ACCACTATCTGCCGGACGCCGCGAGCCGGTCGGACTGGCGAGCCTCGCCGCTTAACGCAGCATCGCTGGCGGGCGTGGCG
CCGGCCCTGGTGATCCTTGCCGGATACGACGTGTTGTTCGACGAAGGGGCAGCTTATGCCGAGCGGCTCGGCACCGAAGC
GAAGGCAACGACCCGGACCTGGCCCGGACAGATCCATGGCTTCGTATCGAAGAGACGGGCCATTCCGGAGGGGCAAGAGG
CCCTCGAAGCCATCGCAACAGCATGGCGCGCCATGGACCCTGCGCTTGCCCGTTAA

Upstream 100 bases:

>100_bases
CGATCCTGCGTGCCGGCGAAACCGGTCTCGCGCAGACCATTTCGGGTACCGTCGCGCAGCCGAGGGAATTGCGGGAAGAC
GAAGCGAGGAGTTTGCTGGC

Downstream 100 bases:

>100_bases
CGCGGAAACACGGCCTCAAGCCGACTCGCGCATAACCAGTGTTGCCTCGAGGATTACCTTGTTCGTCACGGGGGTCGTTC
CATCATCCTCACTCTCGATA

Product: putative lipase/esterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTQLDESAKRALAWPGDPQTPVESCTPALARQQYLDSFADIQRPLEDVVETLERHAGAIRLKIWRGRAAPRQGAPALLYL
HGGGFVIGAPETHEDICRTLANMAGAVVVSPDYRLAPEHPFPAAIDDCAAALVWMTEQADALGIDPLRVVVAGDSAGGNL
AAVVALLARDGQVPAVIGQVLIYPVTDQLQTSDSYSRYEEDFGLTAAAMKWFRDHYLPDAASRSDWRASPLNAASLAGVA
PALVILAGYDVLFDEGAAYAERLGTEAKATTRTWPGQIHGFVSKRRAIPEGQEALEAIATAWRAMDPALAR

Sequences:

>Translated_311_residues
MTQLDESAKRALAWPGDPQTPVESCTPALARQQYLDSFADIQRPLEDVVETLERHAGAIRLKIWRGRAAPRQGAPALLYL
HGGGFVIGAPETHEDICRTLANMAGAVVVSPDYRLAPEHPFPAAIDDCAAALVWMTEQADALGIDPLRVVVAGDSAGGNL
AAVVALLARDGQVPAVIGQVLIYPVTDQLQTSDSYSRYEEDFGLTAAAMKWFRDHYLPDAASRSDWRASPLNAASLAGVA
PALVILAGYDVLFDEGAAYAERLGTEAKATTRTWPGQIHGFVSKRRAIPEGQEALEAIATAWRAMDPALAR
>Mature_310_residues
TQLDESAKRALAWPGDPQTPVESCTPALARQQYLDSFADIQRPLEDVVETLERHAGAIRLKIWRGRAAPRQGAPALLYLH
GGGFVIGAPETHEDICRTLANMAGAVVVSPDYRLAPEHPFPAAIDDCAAALVWMTEQADALGIDPLRVVVAGDSAGGNLA
AVVALLARDGQVPAVIGQVLIYPVTDQLQTSDSYSRYEEDFGLTAAAMKWFRDHYLPDAASRSDWRASPLNAASLAGVAP
ALVILAGYDVLFDEGAAYAERLGTEAKATTRTWPGQIHGFVSKRRAIPEGQEALEAIATAWRAMDPALAR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=144, Percent_Identity=40.2777777777778, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI206597554, Length=216, Percent_Identity=34.2592592592593, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI21328446, Length=150, Percent_Identity=39.3333333333333, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI61966717, Length=155, Percent_Identity=32.258064516129, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI68299767, Length=325, Percent_Identity=27.6923076923077, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI157041237, Length=100, Percent_Identity=42, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68051721, Length=148, Percent_Identity=30.4054054054054, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI226423947, Length=156, Percent_Identity=28.8461538461538, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1786682, Length=234, Percent_Identity=32.9059829059829, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17567059, Length=268, Percent_Identity=33.5820895522388, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI72001146, Length=160, Percent_Identity=35, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17540028, Length=135, Percent_Identity=37.7777777777778, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=120, Percent_Identity=41.6666666666667, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533410, Length=90, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=90, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=131, Percent_Identity=38.1679389312977, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656076, Length=131, Percent_Identity=38.1679389312977, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20130169, Length=131, Percent_Identity=38.1679389312977, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33349; Mature: 33218

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLDESAKRALAWPGDPQTPVESCTPALARQQYLDSFADIQRPLEDVVETLERHAGAIR
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LKIWRGRAAPRQGAPALLYLHGGGFVIGAPETHEDICRTLANMAGAVVVSPDYRLAPEHP
EEEEECCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCCC
FPAAIDDCAAALVWMTEQADALGIDPLRVVVAGDSAGGNLAAVVALLARDGQVPAVIGQV
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHH
LIYPVTDQLQTSDSYSRYEEDFGLTAAAMKWFRDHYLPDAASRSDWRASPLNAASLAGVA
HEECCCHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
PALVILAGYDVLFDEGAAYAERLGTEAKATTRTWPGQIHGFVSKRRAIPEGQEALEAIAT
HHHHHHHCCHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AWRAMDPALAR
HHHHCCHHHCC
>Mature Secondary Structure 
TQLDESAKRALAWPGDPQTPVESCTPALARQQYLDSFADIQRPLEDVVETLERHAGAIR
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LKIWRGRAAPRQGAPALLYLHGGGFVIGAPETHEDICRTLANMAGAVVVSPDYRLAPEHP
EEEEECCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCCC
FPAAIDDCAAALVWMTEQADALGIDPLRVVVAGDSAGGNLAAVVALLARDGQVPAVIGQV
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHH
LIYPVTDQLQTSDSYSRYEEDFGLTAAAMKWFRDHYLPDAASRSDWRASPLNAASLAGVA
HEECCCHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
PALVILAGYDVLFDEGAAYAERLGTEAKATTRTWPGQIHGFVSKRRAIPEGQEALEAIAT
HHHHHHHCCHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AWRAMDPALAR
HHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]