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The map label for this gene is potI [H]

Identifier: 115353097

GI number: 115353097

Start: 3363687

End: 3364526

Strand: Direct

Name: potI [H]

Synonym: Bamb_3046

Alternate gene names: 115353097

Gene position: 3363687-3364526 (Clockwise)

Preceding gene: 115353096

Following gene: 115353098

Centisome position: 94.58

GC content: 64.29

Gene sequence:

>840_bases
ATGATCAAGCCGAACAAACCGCTGTCGACGGGCGTTCTCGCCTTCGGCTTCCTGTTTCTGTACATCCCGATCATCAGCCT
GGTCGTGTACTCGTTCAACGAGTCGAAGCTGGTGACGGTGTGGTCGGGCTTCTCGCTGAAGTGGTACGCGGCGCTTCTGG
AAGACGACGAGCTGCTCACCGCCGCGTGGCTGTCGCTGAAGATCGGCCTGCTGACGGCCACCGCGTCGGTCGTGATCGGC
ACGTGGGCGGGCTTCGTGCTCGCGCGCTTCGGCCGTTTCAAGGGCTTCACGCTGTACACGGGGATGATCAACGCGCCGCT
GGTGATTCCGGAGGTGATCCAGGGGATCTCGCTGCTGTTGCTGTTCGTTGCGCTGGAGCAGATGTTCGGCTGGCCGAAGG
GGCGCGGGATGGTGACGATCTGGATCGGTCACGTGATGCTGTGCGTGTCGTACGTGGCGATCATCGTGCAGTCGCGCGTG
AAGGAGATGAACAAGTCGCTCGAAGAAGCGGCGCTGGATCTGGGCGCGACGCCGCTGAAGGTGTTCTTCGTGGTGACGCT
GCCGCTGATCTCGCAGGCGCTGCTGTCGGGCTGGCTGCTGTCGTTCACGCTGTCGATCGACGATCTGGTGCTGTCGGCGT
TCCTGTCGGGGCCGGGCTCGACGACGCTGCCGCTGGTGGTGTTCTCGCGCGTGCGGCTCGGCCTGAATCCCGAGATGAAC
GCGCTGGCGACGCTGTTCATCACCGCGGTGACGATCGGCGTGATCGTCGTGAACCAGATGATGATCGCGCGCGAACGGCG
TCGGGTGGGCGACCTGAAGGCGGCGTTCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
GGCCGATGGCGTCCGCGGTGACGGTCGCGATGGTGCTGCTGTTGCTGGTGCCGATGGCGCTGTTCCAGTACTACCAGGTC
AAGGAACTGGAGGGAGCGAA

Downstream 100 bases:

>100_bases
CGCCCCTCCTCCCCATCCATCGATAACAAGACCTGCGCGCCGCGCGTTTGCGGCGCGCACCGAAACGCAGATTGACAGGC
ACACGACAAGGAGAATCCGC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIKPNKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLEDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNQMMIARERRRVGDLKAAFAAA

Sequences:

>Translated_279_residues
MIKPNKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLEDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNQMMIARERRRVGDLKAAFAAA
>Mature_279_residues
MIKPNKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLEDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNQMMIARERRRVGDLKAAFAAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=271, Percent_Identity=56.0885608856088, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1787368, Length=248, Percent_Identity=38.3064516129032, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1787714, Length=245, Percent_Identity=31.8367346938775, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788764, Length=221, Percent_Identity=30.316742081448, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30406; Mature: 30406

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPNKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLEDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNQMMIARERRRVGDLKAAFAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKPNKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLEDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNQMMIARERRRVGDLKAAFAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]