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The map label for this gene is potH [H]

Identifier: 115353096

GI number: 115353096

Start: 3362695

End: 3363690

Strand: Direct

Name: potH [H]

Synonym: Bamb_3045

Alternate gene names: 115353096

Gene position: 3362695-3363690 (Clockwise)

Preceding gene: 115353095

Following gene: 115353097

Centisome position: 94.55

GC content: 63.55

Gene sequence:

>996_bases
ATGATCAGAACTTCCGCTTCCACTCCGTCCGCGCCGGTGTCGACCGGTGCAGTAAGCAGCAGCCCCGGCGCCGGCCGCGC
GCGCCGCAGCCGCTTCGCCGCGCTGTCGCGCTTCCTGCCGTCGGGCCGCAACGTCGCGATCGGCGTGCCGTTCCTGTGGC
TCACGATCTTCTTCGCACTGCCGTTCGTGCTGGTGTTCAAGATCAGCTTCGCCGACCAGGTGATGGGGATCCCGCCGTAC
ACGTCGCTCGTCCAATTCAAGGACGGCGTCGTGCACTTCGCGCTGCAGCTGTCGCACTACGCGTTCCTGCTGCAGGACGA
CCTGTACATCGCGACCTACCTCAGCTCGCTGAAGATGGCCACCGTGTCGACGGTGCTGTGCCTGCTGATCGGTTACCCGA
TGGCGTACTACATCGCCCGTGCCGAACCGAGCCGGCGCAACGTGCTGATGATGGCCGTGATGCTGCCGTTCTGGACGTCG
TTCCTGATCCGCGTGTACGCATGGATCGGCATCCTGAAGGACGACGGCCTGCTGAATCACACGCTGATCGCGCTGGGCAT
CATCCACACGCCGCTGCGCCTTTATCACAGCGACGCGGGCGTCTACATCGGGATGGTCTATTCGTACCTGCCGTTCATGA
TCATGCCGCTCTATGCGCACCTCGTGAAGATGGATCTCACGCTGCTCGAGGCCGCGTACGACCTCGGGGCGAAGCCGTGG
GTCGCGTTCACGCGCATCACGCTGCCGCTGTCGAAGAACGGGATCATCGCCGGCAGCCTGCTGGTGTTCATTCCGGCCGT
CGGCGAATACGTGATCCCGGAACTGCTCGGCGGTGCCGACACGCTGATGATCGGCCGCGTGATGTGGGATGAGTTCTTCA
ACAACATGGACTGGCCGATGGCGTCCGCGGTGACGGTCGCGATGGTGCTGCTGTTGCTGGTGCCGATGGCGCTGTTCCAG
TACTACCAGGTCAAGGAACTGGAGGGAGCGAAATGA

Upstream 100 bases:

>100_bases
AGCTCGCACCTGCTGCACGACGGTGCGCCGTCGTGGAACGACGACGTGTTCGTGTCGTGGTCGCCGGCCAGCGGCGTCGT
GCTGACGCAGTGAGGACGAC

Downstream 100 bases:

>100_bases
TCAAGCCGAACAAACCGCTGTCGACGGGCGTTCTCGCCTTCGGCTTCCTGTTTCTGTACATCCCGATCATCAGCCTGGTC
GTGTACTCGTTCAACGAGTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIRTSASTPSAPVSTGAVSSSPGAGRARRSRFAALSRFLPSGRNVAIGVPFLWLTIFFALPFVLVFKISFADQVMGIPPY
TSLVQFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMATVSTVLCLLIGYPMAYYIARAEPSRRNVLMMAVMLPFWTS
FLIRVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPW
VAFTRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQ
YYQVKELEGAK

Sequences:

>Translated_331_residues
MIRTSASTPSAPVSTGAVSSSPGAGRARRSRFAALSRFLPSGRNVAIGVPFLWLTIFFALPFVLVFKISFADQVMGIPPY
TSLVQFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMATVSTVLCLLIGYPMAYYIARAEPSRRNVLMMAVMLPFWTS
FLIRVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPW
VAFTRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQ
YYQVKELEGAK
>Mature_331_residues
MIRTSASTPSAPVSTGAVSSSPGAGRARRSRFAALSRFLPSGRNVAIGVPFLWLTIFFALPFVLVFKISFADQVMGIPPY
TSLVQFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMATVSTVLCLLIGYPMAYYIARAEPSRRNVLMMAVMLPFWTS
FLIRVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPW
VAFTRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQ
YYQVKELEGAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=312, Percent_Identity=51.9230769230769, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1787369, Length=279, Percent_Identity=38.7096774193548, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787713, Length=213, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36702; Mature: 36702

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTSASTPSAPVSTGAVSSSPGAGRARRSRFAALSRFLPSGRNVAIGVPFLWLTIFFAL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHH
PFVLVFKISFADQVMGIPPYTSLVQFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMA
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TVSTVLCLLIGYPMAYYIARAEPSRRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNH
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VAFTRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPM
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH
ASAVTVAMVLLLLVPMALFQYYQVKELEGAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIRTSASTPSAPVSTGAVSSSPGAGRARRSRFAALSRFLPSGRNVAIGVPFLWLTIFFAL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHH
PFVLVFKISFADQVMGIPPYTSLVQFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMA
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TVSTVLCLLIGYPMAYYIARAEPSRRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNH
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VAFTRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPM
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH
ASAVTVAMVLLLLVPMALFQYYQVKELEGAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]