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The map label for this gene is queF

Identifier: 115352839

GI number: 115352839

Start: 3076441

End: 3077265

Strand: Direct

Name: queF

Synonym: Bamb_2788

Alternate gene names: 115352839

Gene position: 3076441-3077265 (Clockwise)

Preceding gene: 115352838

Following gene: 115352843

Centisome position: 86.5

GC content: 65.58

Gene sequence:

>825_bases
ATGAATCCCGAACATTCCCCGCTCGGCAAGGCCACCGTCTATGCCGCCCAGTACGACGCGTCGCTGCTGTTCCCGATCCC
GCGCGCCGGCGCGCGCGAGCAGCTCGGCATCACGTCGGCGCTGCCGTTCTTCGGCACCGACATCTGGAACGCGTACGAAC
TGTCGTGGCTCAACGCGCGCGGCAAGCCGCAGGTCGCGGTCGCGACGTTCTACGTGCCGGCCGAATCGCCGAACATCGTC
GAATCGAAGTCGTTCAAGCTGTATCTCGGCTCGTTCGCCCAATCGAAGTTCGACTCGCTCGACGCGGTGCGCGACACGTT
GAAGCGCGACGTGTCGGCCGCGTGCGGCGCGAGCGTGTCGGTGCAGCTCGTGTCGCCGCACGACTTCGGCAAGCTTCAGA
TGGAAGAGCTCGACGGGCTGTCGCTCGACCGGCTCGACCTCGACACCGACGTGTACGAACCCGATCCGTCGCTGCTGTCG
GCCGCGCAGGACGAAGCGCCGGTGGAGGAGACGCTCGTGTCCGACCTGCTGCGCTCGAACTGCCCGGTCACCGGCCAGCC
CGACTGGGGCAGCGTGCAGATCCACTACGTGGGGCCGCAGATCGACCATGCGGGCCTGCTGCGCTACATCATTTCGTTCC
GCAATCACACGGGCTTTCACGAGCAGTGCGTCGAGCGCATCTTCCTCGACATCCTGCACGCGTGCAAACCGTTGAAGCTG
GCGGTGTATGCGCGCTATACGCGCCGCGGCGGGCTGGACATCAACCCGTTCCGTACGAATTACAACCAGCCGATGCCGGA
TAATGCGCGGACTGCGCGGCAGTAA

Upstream 100 bases:

>100_bases
CGCCGAGTTCGAACGCTTCCTCGCGGCGCTCGGCTATCCGTATGTCGAAGAGAGCGCCAATCCGGCGTACCGGCTCTTCC
TGTCGTAAAGGCTTCGTGCA

Downstream 100 bases:

>100_bases
CTGAAGCGGTATTGGCGGGCAATCGCCGCGAGCCGCAGCCACCGGCAATCACGAGCCCGTAACGAAACAGCCCCGCCGGC
TCGCGCCGACAGGGCTGTTT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQVAVATFYVPAESPNIV
ESKSFKLYLGSFAQSKFDSLDAVRDTLKRDVSAACGASVSVQLVSPHDFGKLQMEELDGLSLDRLDLDTDVYEPDPSLLS
AAQDEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPLKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ

Sequences:

>Translated_274_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQVAVATFYVPAESPNIV
ESKSFKLYLGSFAQSKFDSLDAVRDTLKRDVSAACGASVSVQLVSPHDFGKLQMEELDGLSLDRLDLDTDVYEPDPSLLS
AAQDEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPLKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
>Mature_274_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQVAVATFYVPAESPNIV
ESKSFKLYLGSFAQSKFDSLDAVRDTLKRDVSAACGASVSVQLVSPHDFGKLQMEELDGLSLDRLDLDTDVYEPDPSLLS
AAQDEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPLKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=269, Percent_Identity=53.5315985130111, Blast_Score=277, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 30416; Mature: 30416

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHCCCHHCCCHHCCCCCCCEEEEEECCC
GKPQVAVATFYVPAESPNIVESKSFKLYLGSFAQSKFDSLDAVRDTLKRDVSAACGASVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VQLVSPHDFGKLQMEELDGLSLDRLDLDTDVYEPDPSLLSAAQDEAPVEETLVSDLLRSN
EEEECCCCCCCEEHHHCCCCCCCCEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPLKL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
EEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHCCCHHCCCHHCCCCCCCEEEEEECCC
GKPQVAVATFYVPAESPNIVESKSFKLYLGSFAQSKFDSLDAVRDTLKRDVSAACGASVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VQLVSPHDFGKLQMEELDGLSLDRLDLDTDVYEPDPSLLSAAQDEAPVEETLVSDLLRSN
EEEECCCCCCCEEHHHCCCCCCCCEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPLKL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
AVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
EEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA