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The map label for this gene is citZ [H]

Identifier: 115352633

GI number: 115352633

Start: 2845122

End: 2846354

Strand: Direct

Name: citZ [H]

Synonym: Bamb_2582

Alternate gene names: 115352633

Gene position: 2845122-2846354 (Clockwise)

Preceding gene: 115352631

Following gene: 115352635

Centisome position: 80.0

GC content: 75.26

Gene sequence:

>1233_bases
ATGACGTCCCTCAGCGCGCCGGAAGCCGCCGGCATCCTCGGTGTCAGCGTGAGCACCCTTTATGCGTATGTGAGCCGCGG
GCTGCTGCGCTCGCTGCCCGACGGCGCGAGCAAGCGCCGCCGCTACGACGCGGACGAGGTGCGTCTGCTCGCGCGCCGTC
GCGCCGATGCCAAACGCGCGGGCGGCGTCGCCGAACGCTCGCTCGACTGGGGCGTGCCCGTGCTCGAATCGCGGATCACG
CAGATCGCCGACGGCCGCCTGCACTACCGCGGCGCCGATGCGATCGCGCTCGCGAACGACGCGACGCTCGAGGAAGTGGC
CGCACGGTTGTGGGATTGTCCGCCCGCGCGGCTGGCCGCCGCATCGCCGGCGTCGACCGGTTTCGACACCGCGCAGTGGG
ACGACTGGGCGCGCCGCTGGGCGCATCTGGCGCCGCTCGAGCGCACGCTGGTGCTGCTCCCCGCCGCGGCCGCGTCGCTG
CCGCGCCTGTGGGCGCAGGGGCGCGACGCGCAGTTCGACGCGGCCACGCTGCTGCTGCGCGTGACGGCGGCCGCGCTGGC
CGGCAGCACGCCGAACGACGCGCCCGTGCACCGGCAGCTCGCCGCCGCGTGGCGCATCCGGCGGCGCGACGAAGTCGACC
TGCTGCGCCGCGCGCTCGTGCTGTGCGCCGACCACGAGCTGAACCCGTCGACGTTTGCGGTGCGCTGCATCGCATCGACC
GGCACCCACCTGTTCGGCGCGATCGCGGGCGGGCTCGCCGCGCTGTCGGGCCCGCGTCACGGCGGCGAGACGTTCCGTGC
GGGCGCCCTGCTCGATGAAGCGGCGCGTGCCGCCGACCTCGACCGCTATCTCGCGCTGCGCGTCGCGCACGACGAACGCG
CGGACGGCGCCCGCACGGTGCTGTCCGGCTTCGCGCACCCGCTCTATCCCGACGGCGATCCGCGTGCCCGCGCGTTGCTC
GACGCACTGCACGCGACGCTGCCCGACCGGGCGCCGCTCCAGGTCGCTCGCGCGCTCGCCACGCGCGTGGAAGCGGCGAC
CGGCCTGCGCCCGTCGATCGATTTCGCGCTCGCGGTGCTGGAGCGCACGCTTGCGCTGCCCGACGGCGCCGCGTTCACGC
TGTTCGCGGCCGGCCGCACCGCGGGCTGGATCGCGCATGCGCTCGAACAGTATGCGGACGGCAAGCTGATCCGGCCTCGC
GCGCGCTACGTCGGCAGCGATGCGACGGCCTGA

Upstream 100 bases:

>100_bases
AATTCGCGACGGGTGCGATCAATCTTGATTGCCCGAATCAATGCATAACGAATAGTTGTAGCCGGGCCGCGTTTCATGCG
CCCCGACAGGAGCGATCCGC

Downstream 100 bases:

>100_bases
GCGCGGCCGGCGCGCCATGCGGCAAACCGTCGCCACGCGATCGGCGTCGCGTGCACGGCCGGCGCCGCCTGCGGGCCGAA
CGCGAACACCGCACCGCCCG

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTSLSAPEAAGILGVSVSTLYAYVSRGLLRSLPDGASKRRRYDADEVRLLARRRADAKRAGGVAERSLDWGVPVLESRIT
QIADGRLHYRGADAIALANDATLEEVAARLWDCPPARLAAASPASTGFDTAQWDDWARRWAHLAPLERTLVLLPAAAASL
PRLWAQGRDAQFDAATLLLRVTAAALAGSTPNDAPVHRQLAAAWRIRRRDEVDLLRRALVLCADHELNPSTFAVRCIAST
GTHLFGAIAGGLAALSGPRHGGETFRAGALLDEAARAADLDRYLALRVAHDERADGARTVLSGFAHPLYPDGDPRARALL
DALHATLPDRAPLQVARALATRVEAATGLRPSIDFALAVLERTLALPDGAAFTLFAAGRTAGWIAHALEQYADGKLIRPR
ARYVGSDATA

Sequences:

>Translated_410_residues
MTSLSAPEAAGILGVSVSTLYAYVSRGLLRSLPDGASKRRRYDADEVRLLARRRADAKRAGGVAERSLDWGVPVLESRIT
QIADGRLHYRGADAIALANDATLEEVAARLWDCPPARLAAASPASTGFDTAQWDDWARRWAHLAPLERTLVLLPAAAASL
PRLWAQGRDAQFDAATLLLRVTAAALAGSTPNDAPVHRQLAAAWRIRRRDEVDLLRRALVLCADHELNPSTFAVRCIAST
GTHLFGAIAGGLAALSGPRHGGETFRAGALLDEAARAADLDRYLALRVAHDERADGARTVLSGFAHPLYPDGDPRARALL
DALHATLPDRAPLQVARALATRVEAATGLRPSIDFALAVLERTLALPDGAAFTLFAAGRTAGWIAHALEQYADGKLIRPR
ARYVGSDATA
>Mature_409_residues
TSLSAPEAAGILGVSVSTLYAYVSRGLLRSLPDGASKRRRYDADEVRLLARRRADAKRAGGVAERSLDWGVPVLESRITQ
IADGRLHYRGADAIALANDATLEEVAARLWDCPPARLAAASPASTGFDTAQWDDWARRWAHLAPLERTLVLLPAAAASLP
RLWAQGRDAQFDAATLLLRVTAAALAGSTPNDAPVHRQLAAAWRIRRRDEVDLLRRALVLCADHELNPSTFAVRCIASTG
THLFGAIAGGLAALSGPRHGGETFRAGALLDEAARAADLDRYLALRVAHDERADGARTVLSGFAHPLYPDGDPRARALLD
ALHATLPDRAPLQVARALATRVEAATGLRPSIDFALAVLERTLALPDGAAFTLFAAGRTAGWIAHALEQYADGKLIRPRA
RYVGSDATA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786939, Length=203, Percent_Identity=35.4679802955665, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786527, Length=196, Percent_Identity=32.6530612244898, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 43783; Mature: 43652

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSAPEAAGILGVSVSTLYAYVSRGLLRSLPDGASKRRRYDADEVRLLARRRADAKRA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHH
GGVAERSLDWGVPVLESRITQIADGRLHYRGADAIALANDATLEEVAARLWDCPPARLAA
CCCHHHCCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCHHHHHHHHHHCCCHHHHCC
ASPASTGFDTAQWDDWARRWAHLAPLERTLVLLPAAAASLPRLWAQGRDAQFDAATLLLR
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHEEHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VTAAALAGSTPNDAPVHRQLAAAWRIRRRDEVDLLRRALVLCADHELNPSTFAVRCIAST
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHC
GTHLFGAIAGGLAALSGPRHGGETFRAGALLDEAARAADLDRYLALRVAHDERADGARTV
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LSGFAHPLYPDGDPRARALLDALHATLPDRAPLQVARALATRVEAATGLRPSIDFALAVL
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ERTLALPDGAAFTLFAAGRTAGWIAHALEQYADGKLIRPRARYVGSDATA
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEECCHHHHCCCCCCC
>Mature Secondary Structure 
TSLSAPEAAGILGVSVSTLYAYVSRGLLRSLPDGASKRRRYDADEVRLLARRRADAKRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHH
GGVAERSLDWGVPVLESRITQIADGRLHYRGADAIALANDATLEEVAARLWDCPPARLAA
CCCHHHCCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCHHHHHHHHHHCCCHHHHCC
ASPASTGFDTAQWDDWARRWAHLAPLERTLVLLPAAAASLPRLWAQGRDAQFDAATLLLR
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHEEHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VTAAALAGSTPNDAPVHRQLAAAWRIRRRDEVDLLRRALVLCADHELNPSTFAVRCIAST
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHC
GTHLFGAIAGGLAALSGPRHGGETFRAGALLDEAARAADLDRYLALRVAHDERADGARTV
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LSGFAHPLYPDGDPRARALLDALHATLPDRAPLQVARALATRVEAATGLRPSIDFALAVL
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ERTLALPDGAAFTLFAAGRTAGWIAHALEQYADGKLIRPRARYVGSDATA
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEECCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006016; 12397186 [H]