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The map label for this gene is rnfB [H]

Identifier: 115352416

GI number: 115352416

Start: 2612166

End: 2613185

Strand: Direct

Name: rnfB [H]

Synonym: Bamb_2365

Alternate gene names: 115352416

Gene position: 2612166-2613185 (Clockwise)

Preceding gene: 115352414

Following gene: 115352417

Centisome position: 73.45

GC content: 70.88

Gene sequence:

>1020_bases
ATGCGGGAAATCGGCGATAATCAAGGTTTGGCAAACCTTTCCGGCCGCGCCACACGCGGCCGAGAGCCATTCGGCGCCAC
AGCGCCCGTTGTCACCGTGACTGATTCCAAGACCCTCGCGGATCGCATCGAAGATCTGCTTCCCCAGACGCAATGCACGA
AGTGCGGCTATAACGGCTGCCGTCCGTACGCCGACGCGATCGCCGCCGGCGACGCGAACTACAACCAGTGCCCGCCCGGC
GGCGCCGAAGGCATCGCGCGCCTCGCGAGCCTGCTCGGCAAGCCCGTGATCCCGCTGAACCCCGTCAACGGCACCGAGCA
TCCGCGCGCCGTCGCGTTCATCGACGAAAGCCTGTGCATCGGCTGCACGCTGTGCATGCAGGCGTGCCCGGTCGACGCGA
TCGTCGGCGCACCGAAACAGATGCACACGATCGTCGAATCGCTGTGCACGGGCTGCGACCTGTGCGTGCCGCCCTGCCCG
GTCGACTGCATCGCGATGGTCCCCGTGACCGGCGAGCGCACCGGCTGGGACGCGTGGTCGCAGCAACAGGCGGACGCCGC
GCGCGAGCGCCACGACCGCCGGCTCGCCCGCCAGCGCCGCGAACGCGAGGCCGCCGAAGCGCGCGCCGCCGCCCGCCGCG
CGGCCAGCGCCAGTGCGGCCAAGGCGGCGCCGGAGGCGGCAGAGCCCGGCAAACAGCCTGACGCACCGAGCGCCGCCCCG
GCCGACGACGCCGACGCGAAGAAACGCGCGATCATCGCCGCCGCGCTCGAACGCGCGCGCAAGAAGAAGGAAGAACTGTC
CGAGCAAGGCACCGGGCCCAGGAACACCGAACGCGTGAGCGCGGCCGTGCAGGCGCAGATCGACGCGGCCGAAGCCCGCC
GCAAGCGGCTCGCCGAGCAGCAGGCGCAGCGCGACGCGCAAGCCGCGGCAGCCGGCGACGAAAACGAAAGCGACAACGAC
GATCCAGACAACAACGACGACCGGGCCAGTCCGTCCGCGCCGCCCAAGAACCGTCCATGA

Upstream 100 bases:

>100_bases
CGCCGGCCGCCCTGGAACGGGCGCACCACGTGCGGCCGCCCTCCTGCTGAGCCCCTGCAAAAAAGCGGTTCGATTATGCG
CACAGACGGCGTCCAGCGCA

Downstream 100 bases:

>100_bases
ACGCCACCAAACGACGCGCGATCTACGAAACGCTGCAAAGCCTCAATCCGCATCCGACCACCGAGCTCGAATACACGACG
CCGTTCGAGTTGCTGATCGC

Product: ferredoxin

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MREIGDNQGLANLSGRATRGREPFGATAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYADAIAAGDANYNQCPPG
GAEGIARLASLLGKPVIPLNPVNGTEHPRAVAFIDESLCIGCTLCMQACPVDAIVGAPKQMHTIVESLCTGCDLCVPPCP
VDCIAMVPVTGERTGWDAWSQQQADAARERHDRRLARQRREREAAEARAAARRAASASAAKAAPEAAEPGKQPDAPSAAP
ADDADAKKRAIIAAALERARKKKEELSEQGTGPRNTERVSAAVQAQIDAAEARRKRLAEQQAQRDAQAAAAGDENESDND
DPDNNDDRASPSAPPKNRP

Sequences:

>Translated_339_residues
MREIGDNQGLANLSGRATRGREPFGATAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYADAIAAGDANYNQCPPG
GAEGIARLASLLGKPVIPLNPVNGTEHPRAVAFIDESLCIGCTLCMQACPVDAIVGAPKQMHTIVESLCTGCDLCVPPCP
VDCIAMVPVTGERTGWDAWSQQQADAARERHDRRLARQRREREAAEARAAARRAASASAAKAAPEAAEPGKQPDAPSAAP
ADDADAKKRAIIAAALERARKKKEELSEQGTGPRNTERVSAAVQAQIDAAEARRKRLAEQQAQRDAQAAAAGDENESDND
DPDNNDDRASPSAPPKNRP
>Mature_339_residues
MREIGDNQGLANLSGRATRGREPFGATAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYADAIAAGDANYNQCPPG
GAEGIARLASLLGKPVIPLNPVNGTEHPRAVAFIDESLCIGCTLCMQACPVDAIVGAPKQMHTIVESLCTGCDLCVPPCP
VDCIAMVPVTGERTGWDAWSQQQADAARERHDRRLARQRREREAAEARAAARRAASASAAKAAPEAAEPGKQPDAPSAAP
ADDADAKKRAIIAAALERARKKKEELSEQGTGPRNTERVSAAVQAQIDAAEARRKRLAEQQAQRDAQAAAAGDENESDND
DPDNNDDRASPSAPPKNRP

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=142, Percent_Identity=50.7042253521127, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 35826; Mature: 35826

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIGDNQGLANLSGRATRGREPFGATAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCC
RPYADAIAAGDANYNQCPPGGAEGIARLASLLGKPVIPLNPVNGTEHPRAVAFIDESLCI
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHH
GCTLCMQACPVDAIVGAPKQMHTIVESLCTGCDLCVPPCPVDCIAMVPVTGERTGWDAWS
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
QQQADAARERHDRRLARQRREREAAEARAAARRAASASAAKAAPEAAEPGKQPDAPSAAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ADDADAKKRAIIAAALERARKKKEELSEQGTGPRNTERVSAAVQAQIDAAEARRKRLAEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QAQRDAQAAAAGDENESDNDDPDNNDDRASPSAPPKNRP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MREIGDNQGLANLSGRATRGREPFGATAPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCC
RPYADAIAAGDANYNQCPPGGAEGIARLASLLGKPVIPLNPVNGTEHPRAVAFIDESLCI
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHH
GCTLCMQACPVDAIVGAPKQMHTIVESLCTGCDLCVPPCPVDCIAMVPVTGERTGWDAWS
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
QQQADAARERHDRRLARQRREREAAEARAAARRAASASAAKAAPEAAEPGKQPDAPSAAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ADDADAKKRAIIAAALERARKKKEELSEQGTGPRNTERVSAAVQAQIDAAEARRKRLAEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QAQRDAQAAAAGDENESDNDDPDNNDDRASPSAPPKNRP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA