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The map label for this gene is nth [H]

Identifier: 115352417

GI number: 115352417

Start: 2613182

End: 2613826

Strand: Direct

Name: nth [H]

Synonym: Bamb_2366

Alternate gene names: 115352417

Gene position: 2613182-2613826 (Clockwise)

Preceding gene: 115352416

Following gene: 115352419

Centisome position: 73.48

GC content: 65.43

Gene sequence:

>645_bases
ATGAACGCCACCAAACGACGCGCGATCTACGAAACGCTGCAAAGCCTCAATCCGCATCCGACCACCGAGCTCGAATACAC
GACGCCGTTCGAGTTGCTGATCGCGGTGATGCTGTCCGCGCAGGCGACCGACGTGTCGGTCAACAAGGCGATGCGCAAGA
TGTTTCCGGTCGCGAACACCCCGCGACAAATCGTCGCGCTCGGTGAAGAAGGCGTCGCCGACTACATCAAGACGATCGGC
CTGTACCGCACCAAGGCGAAGAACGTGGTCGCCGCGTGCAACATCCTGCTCGAGCGCTACGACGGCGAGGTGCCGGCCGA
TCGCGAAGCGCTCGAGAGCCTGCCGGGCGTCGGCCGCAAGACCGCGAACGTCGTCCTGAACACCGCGTTCGGCCAGCCGA
CGATCGCGGTCGACACGCATATCTTCCGCGTCGCGAACCGCACGGGGCTCGCGCCCGGCAAGGACGTGCGGGCCGTCGAG
GCCGCGCTCGAGAAGTTCACGCCGAAGGAATTCCTGCAGGACGCGCATCACTGGCTGATCCTGCACGGGCGCTATGTGTG
CAAGGCCCGCAAGCCCGAATGCTGGCACTGCGCGATCGAGCCGCTGTGCGAATTCCGGCCGAAGACGCCGCCGCCGAACG
AGTGA

Upstream 100 bases:

>100_bases
CGCAAGCCGCGGCAGCCGGCGACGAAAACGAAAGCGACAACGACGATCCAGACAACAACGACGACCGGGCCAGTCCGTCC
GCGCCGCCCAAGAACCGTCC

Downstream 100 bases:

>100_bases
GCGCGAGCCGCGGCTGACGGGCCGCGCGCGTTGCAACCCGCCGGCCCCATTGCCGCGCCGGCGGCCTTCCCCTTTCCTGC
TATGCGTTGGACGTCGCCGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNATKRRAIYETLQSLNPHPTTELEYTTPFELLIAVMLSAQATDVSVNKAMRKMFPVANTPRQIVALGEEGVADYIKTIG
LYRTKAKNVVAACNILLERYDGEVPADREALESLPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVE
AALEKFTPKEFLQDAHHWLILHGRYVCKARKPECWHCAIEPLCEFRPKTPPPNE

Sequences:

>Translated_214_residues
MNATKRRAIYETLQSLNPHPTTELEYTTPFELLIAVMLSAQATDVSVNKAMRKMFPVANTPRQIVALGEEGVADYIKTIG
LYRTKAKNVVAACNILLERYDGEVPADREALESLPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVE
AALEKFTPKEFLQDAHHWLILHGRYVCKARKPECWHCAIEPLCEFRPKTPPPNE
>Mature_214_residues
MNATKRRAIYETLQSLNPHPTTELEYTTPFELLIAVMLSAQATDVSVNKAMRKMFPVANTPRQIVALGEEGVADYIKTIG
LYRTKAKNVVAACNILLERYDGEVPADREALESLPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVE
AALEKFTPKEFLQDAHHWLILHGRYVCKARKPECWHCAIEPLCEFRPKTPPPNE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=67.7884615384615, Blast_Score=292, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.2033898305085, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=25.8620689655172, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=25.2631578947368, Blast_Score=68, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=30, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23834; Mature: 23834

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATKRRAIYETLQSLNPHPTTELEYTTPFELLIAVMLSAQATDVSVNKAMRKMFPVANT
CCCHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
PRQIVALGEEGVADYIKTIGLYRTKAKNVVAACNILLERYDGEVPADREALESLPGVGRK
CHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVEAALEKFTPKEFLQDAHHWLI
HHHEEEECCCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHEEE
LHGRYVCKARKPECWHCAIEPLCEFRPKTPPPNE
EECEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNATKRRAIYETLQSLNPHPTTELEYTTPFELLIAVMLSAQATDVSVNKAMRKMFPVANT
CCCHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
PRQIVALGEEGVADYIKTIGLYRTKAKNVVAACNILLERYDGEVPADREALESLPGVGRK
CHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVEAALEKFTPKEFLQDAHHWLI
HHHEEEECCCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHEEE
LHGRYVCKARKPECWHCAIEPLCEFRPKTPPPNE
EECEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]