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The map label for this gene is gcvA [H]

Identifier: 115352266

GI number: 115352266

Start: 2440720

End: 2441661

Strand: Direct

Name: gcvA [H]

Synonym: Bamb_2215

Alternate gene names: 115352266

Gene position: 2440720-2441661 (Clockwise)

Preceding gene: 115352263

Following gene: 115352267

Centisome position: 68.63

GC content: 72.51

Gene sequence:

>942_bases
ATGTCAACGCCGCTCGTCCGTCTCCCGTCGCTGGACCTCGTGCGCGGCTTCGTCGCCGTCGGCCGCCGCATGAGCATCAC
GCTCGCCGCGCAGGACTTGTGCGTCACGCAGTCGGCCGTCAGCCGCCAGGTGCATGCGCTCGAAGCGCATCTCGGCGTCG
CGCTGCTGCAGCGCGGCTATCGGAAGATCGCGTTCACGCCCGAAGGCGAGCGGCTGTTCCGCGCAGCGGATGCGGCGCTG
CTCAGCCTGCAGGACACCGTCGCGTCGCTCGCCGCCGCGCGCGAGCGCCAGCCCGTCACGATTACCGCGAGCATCGGCGT
GACCGCCTTGTGGCTGCTGCCGCGACTCGGGCGCCTGCAGGCGCGCCTGCCCGAGGTCGACTTGCGCGTCGCCGCGAACG
ACAAGGTACTCGATTTGCGCGCGGAAGGCATCGACCTCGGGATCCGCTACTGCCCGCGCGAGCGCGCGCCGGCCGGCGCG
GTACGCCTGTTCGACGAGATCGTCGTGCCGGTCGCTCACCCCGCGCTGGCCGCCCGGCCCGTCACCGACACGGCTGCGAT
CGCGGACCACGTGCTGCTCGAATTCGACGGACCGCCGCAAACGCAGCTGCAATGGCGTGCGCACCTGCACGCAGCCGGCC
TCGGCGACGCGCGCCCCAAGGGCGTGCTGCGCTTCAACCAGTACGACCAGGTGATCCACGCCGCGATCGCCGGCCAGGGC
GTCGCGCTCGGACGGCTCGCGCTCGTCGCGCCGATGCTCGCCGACGGCCGGCTCGCGGTGCTCGGCCCGCGCTCGCAGGC
GTCGTCCGATGCCTATGGCTACTGGCTGTTCCGGCACGATCCGGCACCGCGCCGCGAAGTCGCCGACGTGCGCGACTGGA
TTCTCGCCGAAGCAGCCGAGTGCGACGCGGCGATGCGCGCGTACGACGTGGAGCAGCGGTAG

Upstream 100 bases:

>100_bases
TGACCTGCGCCGGCGCCTGCCCGTCGGCCAGCCTCCATTGAACTTGACGCGTCCGGCGTTCTCAACCAATCTTGCTGCTC
CGTTCGCATGAGCCCTACGC

Downstream 100 bases:

>100_bases
GCGCGCGATTTTTCGCGTTGAGCCGATGCCGGTGCCTGCGCGCAGCGGCGATCGTTGCGCGACGGATCGCGCCTGCAGGC
GATGGACCGACGCCGTAACA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGYRKIAFTPEGERLFRAADAAL
LSLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPEVDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGA
VRLFDEIVVPVAHPALAARPVTDTAAIADHVLLEFDGPPQTQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIHAAIAGQG
VALGRLALVAPMLADGRLAVLGPRSQASSDAYGYWLFRHDPAPRREVADVRDWILAEAAECDAAMRAYDVEQR

Sequences:

>Translated_313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGYRKIAFTPEGERLFRAADAAL
LSLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPEVDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGA
VRLFDEIVVPVAHPALAARPVTDTAAIADHVLLEFDGPPQTQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIHAAIAGQG
VALGRLALVAPMLADGRLAVLGPRSQASSDAYGYWLFRHDPAPRREVADVRDWILAEAAECDAAMRAYDVEQR
>Mature_312_residues
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGYRKIAFTPEGERLFRAADAALL
SLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPEVDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGAV
RLFDEIVVPVAHPALAARPVTDTAAIADHVLLEFDGPPQTQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIHAAIAGQGV
ALGRLALVAPMLADGRLAVLGPRSQASSDAYGYWLFRHDPAPRREVADVRDWILAEAAECDAAMRAYDVEQR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=31.6498316498317, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=30.7958477508651, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=30.078125, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87081978, Length=280, Percent_Identity=28.2142857142857, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786401, Length=255, Percent_Identity=27.843137254902, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787589, Length=232, Percent_Identity=29.3103448275862, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI145693193, Length=248, Percent_Identity=27.0161290322581, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI157672245, Length=278, Percent_Identity=29.4964028776978, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33946; Mature: 33814

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGY
CCCCCEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKIAFTPEGERLFRAADAALLSLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
ARLPEVDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGAVRLFDEIVVPVAHPALAARP
HCCCCCEEEEECCCCEEEEEECCEEECEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCC
VTDTAAIADHVLLEFDGPPQTQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIHAAIAGQG
CCHHHHHHCEEEEEECCCCCCHHHHHHHEEECCCCCCCCCCEEEECHHHHHHHHHHHCCC
VALGRLALVAPMLADGRLAVLGPRSQASSDAYGYWLFRHDPAPRREVADVRDWILAEAAE
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
CDAAMRAYDVEQR
HHHHHHHHCCCCC
>Mature Secondary Structure 
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGY
CCCCEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKIAFTPEGERLFRAADAALLSLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
ARLPEVDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGAVRLFDEIVVPVAHPALAARP
HCCCCCEEEEECCCCEEEEEECCEEECEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCC
VTDTAAIADHVLLEFDGPPQTQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIHAAIAGQG
CCHHHHHHCEEEEEECCCCCCHHHHHHHEEECCCCCCCCCCEEEECHHHHHHHHHHHCCC
VALGRLALVAPMLADGRLAVLGPRSQASSDAYGYWLFRHDPAPRREVADVRDWILAEAAE
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
CDAAMRAYDVEQR
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]