| Definition | Burkholderia ambifaria AMMD chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008390 |
| Length | 3,556,545 |
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The map label for this gene is rluF [H]
Identifier: 115352106
GI number: 115352106
Start: 2251285
End: 2253357
Strand: Direct
Name: rluF [H]
Synonym: Bamb_2055
Alternate gene names: 115352106
Gene position: 2251285-2253357 (Clockwise)
Preceding gene: 115352105
Following gene: 115352112
Centisome position: 63.3
GC content: 71.64
Gene sequence:
>2073_bases ATGCGCACCAAATTAACCGTCAAGAATCCGCGTCCGGCGTCGCCGACCCGCGCACCCGTCCGCTCCGGCAGCCTCGTCGC TCGCAAGGCGGTGCGCCCCGCCGGGCCGCCCGCGGCCGACAAGCCGCCGCGCCCGAAGAAGGCCGCCGCGCCGGCAGCCG CCGGCGAACGCGCGTTCAAGCCGCGCGGCGCGGAACGCCCGGGTGCGGATCGCGCGCCGGCGAAGCGCGCACCGCGTGAC GGCGGTGCCGAGCGCGGCGACGCGCGTCCCGCGCGTCCGTATCGCAGCGCCGAAGGTCAGCCCGATGCGCGGCCGCGCCG CGCGGGCGCCGAAGGCGGTGCCCGCGCGCCGTATCGCGACAAGGCGGAGGGCTACGGCGAGAAGCGCAGCTTCGGCGAAC GCCGTACGTCGTCCGACCGCCCGGCTCGCCGCAGCGATGACGATACGCGGCCGCGCCGTGCAGGCGCGGAAGGCGGCGCA CGCGCACCGTATCGTGACAAGGCAACCGGCGAAGGCGCGAAGCGCAGTTTCGACGATCGCCGTACGTCGTCCGACCGCCC GGCTCGCCGCAGCGACGACGATACGCGGCCGCGCCGCGCAGGCGCCGAAGGCGGTGCACGCGCGCCGTATCGCGACAAGG CAACCGGCGATGCGCCGAAGCGCAGCTTCGGTGAACGCCGTACGTCGTCCGACCGTCCGGCACGCCGCGACGACGATGCA CGGCCGCGCCGCGCAGGTGCCGAAGGCGGTGCACGCGCACCGTATCGCGACAAGGCAACCGGCGATGCGCCGAAGCGCAG CTTCGGTGAACGCAGTACATCGTCCGACCGACCGGCACGCCGCGACGACGATACGCGGCCGCGTCGCGCAGGCGCCGAAG GCGGCGCTCGCGCACCGTATCGCGACAAGGCAACCGGCGATGCGCCGAAGCGCAGCTTCGGCGAACGCCGTGCGCCGTCC GACCGCCCAGCTCGCCGCAGCGATGACGATACGCGGCCGCGCCGCGCAGGCGCCGAAGGCGGCGCACGCGCACCGTATCG TGACAAGGCAACCGGCGATGCACCGAAGCGCAGCTTCGGTGAACGCAGTACATCGACCGACCGACCGGCTCGCCGCAGCG ACGACGATGCACGGCCGCGCCGCGCAGGCGCCGAAGGCGGCGCACGCGCCCCCTATCGCGACAAGGCAACCGGCTACGGC GAAAAGCGCAGCTTCGGCGAACGCGGCAACACCGAGCGCCGCTCGACGGCCGCCGCGCCGAAAGCCGCACAACCGGTCAA GCACCGCGCCCCCGACATCGATCACGGCGACGAAACCGGCCTGATGCGCCTGTCGAAGCGCATGTCCGAGCTCGGCCTGT GCTCGCGCCGCGAAGCCGACGAATGGATCGAAAAGGGCTGGGTGCTCGTCGACGGCGAACGCATCGACACGCTCGGCACC AAGGTCCGTCCCGACCAGAAAATCGAGATCGACGAACGCGCGAGCGCCGCGCAGGCCGCACAGGTGACGATCCTGCTGCA CAAGCCGGTCGGCTATGTGTCCGGTCAGGCGGAAGACGGCTACGAGCCCGCGTCGGTGCTGATCACGCGCGCGAACCAGT GGAGCGGCGACCGCTCGCCGCTGCGCTTCTCGCCGCAGCATCTGCACGCGCTCGCTCCGGCCGGCCGGCTCGACATCGAT TCGACCGGCATGCTCGTGTTGACGCAGAACGGCCGGATCGCGAAGCAACTGATCGGCGAGCAGTCGGACATCGACAAGGA GTACCTGGTGCGCGTGCGCTTCGGCGAACGGCTGCTCGACATCGACCAGCACTTCCCCGCCGAATCGCTCGCGAAGCTGC GCCACGGCCTCGAACTCGACGGTGTCGCACTGAAGCCCGCGATGGTGAGCTGGCAGAACGGCGAACAGCTGCGTTTCGTG CTGCGTGAAGGCAAGAAGCGCCAGATCCGCCGGATGTGCGAACTGGTCGGTCTCGAAGTGATCGGCCTGAAGCGCGTGCG GATGGGCCGCGTGATGCTCGGCGCGCTGCCGCAAGGGCAATGGCGCTACCTGTCGCCCGACGAGTCGTTCTGA
Upstream 100 bases:
>100_bases TTCCTGCTCGATGGTGCCGGCCTGTCCGACAACCGCCTGCAGATCCAGCTCGAAACCGAATTGCTGCGCGCGATCGCAGT CTGAATGAATCCCAGCGTTT
Downstream 100 bases:
>100_bases CGGCAGGCAGGCACACACGGCGTGCCACGCAATGACAAAGCCCGCGCAAGCGGGCTTTGTCGTTTCCGTCACGACGACGG CACGCAACATCGCGCACCGC
Product: pseudouridine synthase, Rsu
Products: NA
Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]
Number of amino acids: Translated: 690; Mature: 690
Protein sequence:
>690_residues MRTKLTVKNPRPASPTRAPVRSGSLVARKAVRPAGPPAADKPPRPKKAAAPAAAGERAFKPRGAERPGADRAPAKRAPRD GGAERGDARPARPYRSAEGQPDARPRRAGAEGGARAPYRDKAEGYGEKRSFGERRTSSDRPARRSDDDTRPRRAGAEGGA RAPYRDKATGEGAKRSFDDRRTSSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRTSSDRPARRDDDA RPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSSDRPARRDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRAPS DRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSTDRPARRSDDDARPRRAGAEGGARAPYRDKATGYG EKRSFGERGNTERRSTAAAPKAAQPVKHRAPDIDHGDETGLMRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGT KVRPDQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPASVLITRANQWSGDRSPLRFSPQHLHALAPAGRLDID STGMLVLTQNGRIAKQLIGEQSDIDKEYLVRVRFGERLLDIDQHFPAESLAKLRHGLELDGVALKPAMVSWQNGEQLRFV LREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQGQWRYLSPDESF
Sequences:
>Translated_690_residues MRTKLTVKNPRPASPTRAPVRSGSLVARKAVRPAGPPAADKPPRPKKAAAPAAAGERAFKPRGAERPGADRAPAKRAPRD GGAERGDARPARPYRSAEGQPDARPRRAGAEGGARAPYRDKAEGYGEKRSFGERRTSSDRPARRSDDDTRPRRAGAEGGA RAPYRDKATGEGAKRSFDDRRTSSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRTSSDRPARRDDDA RPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSSDRPARRDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRAPS DRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSTDRPARRSDDDARPRRAGAEGGARAPYRDKATGYG EKRSFGERGNTERRSTAAAPKAAQPVKHRAPDIDHGDETGLMRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGT KVRPDQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPASVLITRANQWSGDRSPLRFSPQHLHALAPAGRLDID STGMLVLTQNGRIAKQLIGEQSDIDKEYLVRVRFGERLLDIDQHFPAESLAKLRHGLELDGVALKPAMVSWQNGEQLRFV LREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQGQWRYLSPDESF >Mature_690_residues MRTKLTVKNPRPASPTRAPVRSGSLVARKAVRPAGPPAADKPPRPKKAAAPAAAGERAFKPRGAERPGADRAPAKRAPRD GGAERGDARPARPYRSAEGQPDARPRRAGAEGGARAPYRDKAEGYGEKRSFGERRTSSDRPARRSDDDTRPRRAGAEGGA RAPYRDKATGEGAKRSFDDRRTSSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRTSSDRPARRDDDA RPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSSDRPARRDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRAPS DRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSTDRPARRSDDDARPRRAGAEGGARAPYRDKATGYG EKRSFGERGNTERRSTAAAPKAAQPVKHRAPDIDHGDETGLMRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGT KVRPDQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPASVLITRANQWSGDRSPLRFSPQHLHALAPAGRLDID STGMLVLTQNGRIAKQLIGEQSDIDKEYLVRVRFGERLLDIDQHFPAESLAKLRHGLELDGVALKPAMVSWQNGEQLRFV LREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQGQWRYLSPDESF
Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]
COG id: COG1187
COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 S4 RNA-binding domain [H]
Homologues:
Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=35.3413654618474, Blast_Score=124, Evalue=1e-29, Organism=Escherichia coli, GI1788510, Length=250, Percent_Identity=30, Blast_Score=78, Evalue=2e-15, Organism=Escherichia coli, GI1787524, Length=254, Percent_Identity=31.496062992126, Blast_Score=75, Evalue=2e-14,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR020103 - InterPro: IPR006145 - InterPro: IPR000748 - InterPro: IPR018496 - InterPro: IPR002942 [H]
Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]
EC number: NA
Molecular weight: Translated: 75744; Mature: 75744
Theoretical pI: Translated: 11.29; Mature: 11.29
Prosite motif: PS50889 S4
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.4 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRTKLTVKNPRPASPTRAPVRSGSLVARKAVRPAGPPAADKPPRPKKAAAPAAAGERAFK CCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC PRGAERPGADRAPAKRAPRDGGAERGDARPARPYRSAEGQPDARPRRAGAEGGARAPYRD CCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC KAEGYGEKRSFGERRTSSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGEGAKRSFDDR HHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH RTSSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRTSSDRPARRDDDA CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC RPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSSDRPARRDDDTRPRRAGAEGGARAPY CHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC RDKATGDAPKRSFGERRAPSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFG CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHC ERSTSTDRPARRSDDDARPRRAGAEGGARAPYRDKATGYGEKRSFGERGNTERRSTAAAP CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCC KAAQPVKHRAPDIDHGDETGLMRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGT CHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHCHHHHHHHHCCCEEECCCCHHHHCC KVRPDQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPASVLITRANQWSGDRSP CCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCC LRFSPQHLHALAPAGRLDIDSTGMLVLTQNGRIAKQLIGEQSDIDKEYLVRVRFGERLLD CCCCHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEHHHHHHHHH IDQHFPAESLAKLRHGLELDGVALKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEV HHHCCCHHHHHHHHHCCCCCCEEECCCEECCCCCHHHHHHHHHCHHHHHHHHHHHHCCHH IGLKRVRMGRVMLGALPQGQWRYLSPDESF HHHHHHHHHHHHHHCCCCCCEEECCCCCCC >Mature Secondary Structure MRTKLTVKNPRPASPTRAPVRSGSLVARKAVRPAGPPAADKPPRPKKAAAPAAAGERAFK CCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC PRGAERPGADRAPAKRAPRDGGAERGDARPARPYRSAEGQPDARPRRAGAEGGARAPYRD CCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC KAEGYGEKRSFGERRTSSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGEGAKRSFDDR HHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH RTSSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFGERRTSSDRPARRDDDA CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC RPRRAGAEGGARAPYRDKATGDAPKRSFGERSTSSDRPARRDDDTRPRRAGAEGGARAPY CHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC RDKATGDAPKRSFGERRAPSDRPARRSDDDTRPRRAGAEGGARAPYRDKATGDAPKRSFG CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHC ERSTSTDRPARRSDDDARPRRAGAEGGARAPYRDKATGYGEKRSFGERGNTERRSTAAAP CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCC KAAQPVKHRAPDIDHGDETGLMRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGT CHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHCHHHHHHHHCCCEEECCCCHHHHCC KVRPDQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPASVLITRANQWSGDRSP CCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCC LRFSPQHLHALAPAGRLDIDSTGMLVLTQNGRIAKQLIGEQSDIDKEYLVRVRFGERLLD CCCCHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEHHHHHHHHH IDQHFPAESLAKLRHGLELDGVALKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEV HHHCCCHHHHHHHHHCCCCCCEEECCCEECCCCCHHHHHHHHHCHHHHHHHHHHHHCCHH IGLKRVRMGRVMLGALPQGQWRYLSPDESF HHHHHHHHHHHHHHCCCCCCEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]