Definition Burkholderia ambifaria AMMD chromosome chromosome 1, complete sequence.
Accession NC_008390
Length 3,556,545

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The map label for this gene is hycF [C]

Identifier: 115352012

GI number: 115352012

Start: 2158706

End: 2158951

Strand: Direct

Name: hycF [C]

Synonym: Bamb_1961

Alternate gene names: 115352012

Gene position: 2158706-2158951 (Clockwise)

Preceding gene: 115352011

Following gene: 115352013

Centisome position: 60.7

GC content: 64.23

Gene sequence:

>246_bases
ATGCCCCATACCCCGCACGACATCTTCCTGCGCAGCAGCGCGCCCGTGACGATCGACGAGGACCGCTGCATCGCCGACAA
GGGCTGCACCGTCTGCGTCGACGTGTGCCCGCTCGACCTGCTCGCGATCGACGTGACCAAGGGCAAGGCGTACATGCAGT
TCGACGAATGCTGGTACTGCATGCCATGCGAACGCGATTGCCCGACCGGCGCGGTCAAGGTGGACATTCCATACCTGCTG
CGCTGA

Upstream 100 bases:

>100_bases
CGTACGTCGTGCCGCTCGCCGACGACGAACGCAGCGCCTACGCGCAGTTGCGCATCCGCGAACCGGCCGCACGCGCGGCC
GCCCTTTGAGGAACCTTGCC

Downstream 100 bases:

>100_bases
TGCCGCGCGCCGCCGGCGCGGCGCTTTCCCACTACCGTGCTCACGACACATGAAACCTGAAACCTGCGCTGCGCGTGCGT
CGACATCAAGCGCCGCTGCC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: Ferredoxin 4Fe-4S; 4Fe-4S Ferredoxin; Ferredoxin-Like Protein

Number of amino acids: Translated: 81; Mature: 80

Protein sequence:

>81_residues
MPHTPHDIFLRSSAPVTIDEDRCIADKGCTVCVDVCPLDLLAIDVTKGKAYMQFDECWYCMPCERDCPTGAVKVDIPYLL
R

Sequences:

>Translated_81_residues
MPHTPHDIFLRSSAPVTIDEDRCIADKGCTVCVDVCPLDLLAIDVTKGKAYMQFDECWYCMPCERDCPTGAVKVDIPYLL
R
>Mature_80_residues
PHTPHDIFLRSSAPVTIDEDRCIADKGCTVCVDVCPLDLLAIDVTKGKAYMQFDECWYCMPCERDCPTGAVKVDIPYLLR

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1146

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 9110; Mature: 8978

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
13.6 %Cys+Met (Translated Protein)
10.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
12.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHTPHDIFLRSSAPVTIDEDRCIADKGCTVCVDVCPLDLLAIDVTKGKAYMQFDECWYC
CCCCCCEEEEECCCCEEECCCCEECCCCCEEEEECCCCEEEEEEECCCCEEEEECCCEEE
MPCERDCPTGAVKVDIPYLLR
CCCCCCCCCCEEEEECCEECC
>Mature Secondary Structure 
PHTPHDIFLRSSAPVTIDEDRCIADKGCTVCVDVCPLDLLAIDVTKGKAYMQFDECWYC
CCCCCEEEEECCCCEEECCCCEECCCCCEEEEECCCCEEEEEEECCCCEEEEECCCEEE
MPCERDCPTGAVKVDIPYLLR
CCCCCCCCCCEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA