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The map label for this gene is rluB [H]

Identifier: 115351432

GI number: 115351432

Start: 1513898

End: 1515616

Strand: Direct

Name: rluB [H]

Synonym: Bamb_1379

Alternate gene names: 115351432

Gene position: 1513898-1515616 (Clockwise)

Preceding gene: 115351431

Following gene: 115351433

Centisome position: 42.57

GC content: 71.26

Gene sequence:

>1719_bases
TTGACAGATATCCACGATATTGAATCGTCCGCGCCTGCCGCACAGGCCGCGCGTGCCGACGATGCGCCGGAGCAGGACGC
TCCGGCCGCGGGTGGTGACGAGCGTCCCCGCCGCGGCCTGCGGCGCGGTCCGCGCAGCCTGATCGCGCGTCGCCGCGCGG
CGGCCAAGTCGAAGGGCGCCGAGGGCGAGTCGCAGGACGGCGAAGGCGCAGACGCGCCGCCGGCCGAGGCCGCCGAAGCG
CAGCCCGCACGCGCACCGCGCAAGGAAGGCGCGGCCAGGGGCGGCCGCAAGCCGGCCGCCAAGCGTGAAGGTGCACCCAA
GAGTGCGCAGGGCGGACAAGGCGGCCAGGGGCGGCGTGGCAGCCCGGCGAAAGCCGAGGGCGGCGCGGCGAAGGTCGAAG
GCGATGCAGCGTCGCAGGACGATCTGTTCGCCTATGTGACGTCGCCGGCCTTCGACGCGGACAACTCCGCGGGCGGCAGC
GGCGTGCGTGCACCGATGCTGCGGCGCGGTCGTGCGCAGCCGACGAACAAGCGCGTGCTGTCGCCGGACGACGACGCACC
GAAGCTTCACAAGGTGCTCGCGGAAGCCGGCATGGGCTCGCGCCGCGAAATGGAAGAGCTGATCGTCGCCGGCCGCGTGT
CGGTGAACGGTGAGCCCGCGCACATCGGCCAGCGCATCATGCCGACCGACCAGGTCCGCATCAATGGCAAGCCGGTCAAG
CGCAAGCTGCCGAACAAGCCGCCGCGCGTGCTGCTGTACCACAAGCCGACGGGCGAGATCGTCAGCCATGCGGATCCGGA
AGGTCGTCCGTCGGTGTTCGATCGCCTGCCGCCGATGAAGACGGCGAAATGGCTCGCGGTGGGCCGCCTCGACTTCAACA
CCGAAGGTCTGCTGATGCTGACCACGTCCGGCGATCTCGCGAACCGCTTCATGCATCCGCGTTATAGCGTCGAGCGCGAG
TATGCGGTGCGTGTCGTCGGCGAGCTGTCCGAAGGCATGCGGCAGAAGCTGCTGCATGGCGTCGAGCTCGATGACGGCCC
GGCGAACTTCCTGCGCATCCGCGACGGCGGCGGCGAAGGCACGAACCACTGGTATCACGTTGCGCTGGCCGAAGGCCGCA
ACCGCGAAGTGCGCCGTATGTTCGAAGCGGCCGGCCTGATGGTGAGCCGCCTGATCCGTACGCGCCACGGTCCGATCCCG
CTGCCGCGCGGCCTGAAGCGCGGCCGTTGGGAAGAGCTCGACGAGGCGCAGGTGCGCAAGCTGATGGCGACCGTCGGCCT
GAAGGCGCCGTCCGAGGAAAAGGGCAAGCGCGGTGGCAGTGGGCCGGCCGAGCGCCGCCAGCCGGATCCGATGCAGACGT
CGATGGGCTTCATCAGCCGTGAGCCGGTGCTGACCACGCATGGTCAACTCGACCAGCCGCGTCGCGGTGGCGGTCGGCGC
GGCGGCCCCGGCCTGCCGGGCCTGAGCGGCTACGGCAGCCTGCCGGTTTCGCCGTCGGGCCATGGCAACCGCTCGGGTGG
CGGTCGCGATGTCGACGGCAATCGCGCGTCGTATGGCGCGGGTCCCAAGCGTGAAGGCGGCGCGGGCGCCAAGCGTGGCG
GCAGCAAGGGCGGCGGTAACCGCAACCCGAACGGCAATCGCGCCGAAGGCGGCGGCAACACCGGTGGTGGTGCACGCGGC
GGTCAGCGCCCGCAGCGCAATCGCTCGCGCGGTCGCTGA

Upstream 100 bases:

>100_bases
GCCGGACGACGAAGCAGAACCCGAACAGCGCCGCGCCTGATCGCGGCCGACTTCTTGCCGCGCCCGCGCCGGGCGCGAGA
CCTGACATTTTGAGGTTGTT

Downstream 100 bases:

>100_bases
ACGTTTCCGGCGGACGGCGGCGTGAAGCCGCCGGCGCGCCGGCGAAATCGGCGTGAATCCGGTTGGGTCGGCACCGCGCG
CTGCGTGACTTGCTTTCGAC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MTDIHDIESSAPAAQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGESQDGEGADAPPAEAAEA
QPARAPRKEGAARGGRKPAAKREGAPKSAQGGQGGQGRRGSPAKAEGGAAKVEGDAASQDDLFAYVTSPAFDADNSAGGS
GVRAPMLRRGRAQPTNKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVK
RKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVERE
YAVRVVGELSEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAAGLMVSRLIRTRHGPIP
LPRGLKRGRWEELDEAQVRKLMATVGLKAPSEEKGKRGGSGPAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRR
GGPGLPGLSGYGSLPVSPSGHGNRSGGGRDVDGNRASYGAGPKREGGAGAKRGGSKGGGNRNPNGNRAEGGGNTGGGARG
GQRPQRNRSRGR

Sequences:

>Translated_572_residues
MTDIHDIESSAPAAQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGESQDGEGADAPPAEAAEA
QPARAPRKEGAARGGRKPAAKREGAPKSAQGGQGGQGRRGSPAKAEGGAAKVEGDAASQDDLFAYVTSPAFDADNSAGGS
GVRAPMLRRGRAQPTNKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVK
RKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVERE
YAVRVVGELSEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAAGLMVSRLIRTRHGPIP
LPRGLKRGRWEELDEAQVRKLMATVGLKAPSEEKGKRGGSGPAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRR
GGPGLPGLSGYGSLPVSPSGHGNRSGGGRDVDGNRASYGAGPKREGGAGAKRGGSKGGGNRNPNGNRAEGGGNTGGGARG
GQRPQRNRSRGR
>Mature_571_residues
TDIHDIESSAPAAQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGESQDGEGADAPPAEAAEAQ
PARAPRKEGAARGGRKPAAKREGAPKSAQGGQGGQGRRGSPAKAEGGAAKVEGDAASQDDLFAYVTSPAFDADNSAGGSG
VRAPMLRRGRAQPTNKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKR
KLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREY
AVRVVGELSEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAAGLMVSRLIRTRHGPIPL
PRGLKRGRWEELDEAQVRKLMATVGLKAPSEEKGKRGGSGPAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRRG
GPGLPGLSGYGSLPVSPSGHGNRSGGGRDVDGNRASYGAGPKREGGAGAKRGGSKGGGNRNPNGNRAEGGGNTGGGARGG
QRPQRNRSRGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=297, Percent_Identity=50.5050505050505, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=29.6442687747036, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788510, Length=218, Percent_Identity=29.3577981651376, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081838, Length=192, Percent_Identity=33.8541666666667, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 60525; Mature: 60393

Theoretical pI: Translated: 11.24; Mature: 11.24

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIHDIESSAPAAQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCC
EGESQDGEGADAPPAEAAEAQPARAPRKEGAARGGRKPAAKREGAPKSAQGGQGGQGRRG
CCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCC
SPAKAEGGAAKVEGDAASQDDLFAYVTSPAFDADNSAGGSGVRAPMLRRGRAQPTNKRVL
CCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
SPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHH
RKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLML
HHCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCEEEE
TTSGDLANRFMHPRYSVEREYAVRVVGELSEGMRQKLLHGVELDDGPANFLRIRDGGGEG
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
TNHWYHVALAEGRNREVRRMFEAAGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRK
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LMATVGLKAPSEEKGKRGGSGPAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRR
HHHHHCCCCCCHHHCCCCCCCCHHCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GGPGLPGLSGYGSLPVSPSGHGNRSGGGRDVDGNRASYGAGPKREGGAGAKRGGSKGGGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RNPNGNRAEGGGNTGGGARGGQRPQRNRSRGR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TDIHDIESSAPAAQAARADDAPEQDAPAAGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCC
EGESQDGEGADAPPAEAAEAQPARAPRKEGAARGGRKPAAKREGAPKSAQGGQGGQGRRG
CCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCC
SPAKAEGGAAKVEGDAASQDDLFAYVTSPAFDADNSAGGSGVRAPMLRRGRAQPTNKRVL
CCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
SPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHH
RKLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLML
HHCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCEEEE
TTSGDLANRFMHPRYSVEREYAVRVVGELSEGMRQKLLHGVELDDGPANFLRIRDGGGEG
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
TNHWYHVALAEGRNREVRRMFEAAGLMVSRLIRTRHGPIPLPRGLKRGRWEELDEAQVRK
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LMATVGLKAPSEEKGKRGGSGPAERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRR
HHHHHCCCCCCHHHCCCCCCCCHHCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GGPGLPGLSGYGSLPVSPSGHGNRSGGGRDVDGNRASYGAGPKREGGAGAKRGGSKGGGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RNPNGNRAEGGGNTGGGARGGQRPQRNRSRGR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]