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The map label for this gene is bkdB [H]

Identifier: 115351178

GI number: 115351178

Start: 1233396

End: 1234733

Strand: Direct

Name: bkdB [H]

Synonym: Bamb_1125

Alternate gene names: 115351178

Gene position: 1233396-1234733 (Clockwise)

Preceding gene: 115351177

Following gene: 115351179

Centisome position: 34.68

GC content: 70.1

Gene sequence:

>1338_bases
ATGGGGATTCATGTGATCAAGATGCCGGATATCGGCGAAGGGATCGCCGAGGTCGAGCTGGTGGCGTGGCACGTGGAAGT
CGGCCAGACGATCAAGGAAGACCAGCCGCTCGCCGACGTGATGACCGACAAGGCGGCGGTGGAGATTCCGTCGCCGGTGG
CGGGCAAAGTGCTGGCGCTCGGCGGCCGGATCGGCGAGATGATGGCGGTCGGCAGCGAGCTGATTCGTGTCGAGGTCGAA
GGCAACGGCAACCTGAAGCCGGGCACGAAGGCACGCGATGCCGAAGCGGATGCGACGTCGCGTCCGGCGGCGGTCGACAC
GCCTGCAAAGTCGTCGAAAGTCACCGAAGCTGCCGAAGCGCACGACGCATCGAAGGCAGCGCGCCACACCGCTGAGCGTG
CACCGGCCGAGCCGCGTCGCACGGAACACGCGGCGGCGCCGCGCGCGGCGCTCGCGCCGGGCGAACGGCCGCTCGCGTCG
CCGGCGGTGCGCCAGCGCGCCTGGGACATGGGTATCGAGCTGCGCTATGTGCGCGGCACCGGCGAGGCCGGCCGGATCCT
GCATGCGGACCTCGATGCGTACGCACGCACGGGCGGCACGGCGGCGGGTGGCGCGCAAGCGCGCGGCTACGACGAGCGCA
ACGATGAAACGGAGGTGCCGGTGATCGGCCTGCGGCGGGCGATCGCGCGCAAGATGCAGGAAGCGAAGCGCCGCATTCCG
CACTTCAGCTACGTCGAGGAGATCGACGTCACCGAGCTCGAATCGCTGCGCAGCGAGCTGAACCGACGCCACGGCGACAC
GCGCGGCCGGCTCACGCCGCTGCCGCTGCTGATCCGCGCGATGGTGATCGCGCTGCGCGACTTCCCGCAGATCAACGCGC
GCTACGACGACGAAGCCGGGGTCGTCACGCGCTACGGCGCGGTGCACATGGGCGTCGCGACGCAGACGGACAGCGGCCTC
ACGGTGCCGGTGCTGCGTCATGCGGAAGCGCGCGACGTGTGGTCGATCTCGGCGGAAATCGCGCGGCTCGCCGACGCCGT
GCGCGCGAACCGCGCGCAACGCGACGAGCTGAGCGGCTCGACGATCACGATCTCGAGCCTCGGCGCGCTCGGCGGCATCA
TGTCGACGCCGGTCATCAACCATCCGGAGGTCGGCATCGTCGGCGTGAACCGCATCGTCGAGCGGCCGATGATCCGCGAC
GGCGCGATCGTCGCGCGCAAGCTGATGAACCTGTCGTCGTCGTTCGACCATCGCGTCGTCGACGGCGCGGACGCGGCCGA
ATTCATCCAGGCCGTGCGCGGGTTGCTCGAGCGCCCGGCGCTGCTGTTCGTGGAGTGA

Upstream 100 bases:

>100_bases
ACGGGCTGGGACACGCCGTATCCGCACGCGCAGGAATGGGCGTATTTCCCCGGCCCGGCCCGGGTTGGCGAAGCATTGCG
ACGCGTGATGGAGGCCTGAG

Downstream 100 bases:

>100_bases
GTGCGATGCCGAACGAACACACCACGCTGCTCGTGATCGGCGGCGGGCCGGGCGGTTACGTCGCCGCGATTCGCGCGGGG
CAGCTCGGCATTCCGACGGT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVAGKVLALGGRIGEMMAVGSELIRVEVE
GNGNLKPGTKARDAEADATSRPAAVDTPAKSSKVTEAAEAHDASKAARHTAERAPAEPRRTEHAAAPRAALAPGERPLAS
PAVRQRAWDMGIELRYVRGTGEAGRILHADLDAYARTGGTAAGGAQARGYDERNDETEVPVIGLRRAIARKMQEAKRRIP
HFSYVEEIDVTELESLRSELNRRHGDTRGRLTPLPLLIRAMVIALRDFPQINARYDDEAGVVTRYGAVHMGVATQTDSGL
TVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIMSTPVINHPEVGIVGVNRIVERPMIRD
GAIVARKLMNLSSSFDHRVVDGADAAEFIQAVRGLLERPALLFVE

Sequences:

>Translated_445_residues
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVAGKVLALGGRIGEMMAVGSELIRVEVE
GNGNLKPGTKARDAEADATSRPAAVDTPAKSSKVTEAAEAHDASKAARHTAERAPAEPRRTEHAAAPRAALAPGERPLAS
PAVRQRAWDMGIELRYVRGTGEAGRILHADLDAYARTGGTAAGGAQARGYDERNDETEVPVIGLRRAIARKMQEAKRRIP
HFSYVEEIDVTELESLRSELNRRHGDTRGRLTPLPLLIRAMVIALRDFPQINARYDDEAGVVTRYGAVHMGVATQTDSGL
TVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIMSTPVINHPEVGIVGVNRIVERPMIRD
GAIVARKLMNLSSSFDHRVVDGADAAEFIQAVRGLLERPALLFVE
>Mature_444_residues
GIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVAGKVLALGGRIGEMMAVGSELIRVEVEG
NGNLKPGTKARDAEADATSRPAAVDTPAKSSKVTEAAEAHDASKAARHTAERAPAEPRRTEHAAAPRAALAPGERPLASP
AVRQRAWDMGIELRYVRGTGEAGRILHADLDAYARTGGTAAGGAQARGYDERNDETEVPVIGLRRAIARKMQEAKRRIPH
FSYVEEIDVTELESLRSELNRRHGDTRGRLTPLPLLIRAMVIALRDFPQINARYDDEAGVVTRYGAVHMGVATQTDSGLT
VPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIMSTPVINHPEVGIVGVNRIVERPMIRDG
AIVARKLMNLSSSFDHRVVDGADAAEFIQAVRGLLERPALLFVE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=464, Percent_Identity=30.1724137931034, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=26.3038548752834, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=29.2035398230088, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI203098816, Length=233, Percent_Identity=27.8969957081545, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI203098753, Length=233, Percent_Identity=27.8969957081545, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=28.9308176100629, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786946, Length=449, Percent_Identity=27.3942093541203, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=459, Percent_Identity=30.9368191721133, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25146366, Length=441, Percent_Identity=26.530612244898, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=457, Percent_Identity=27.5711159737418, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=25.4019292604502, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=445, Percent_Identity=27.8651685393258, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=26.3616557734205, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI18859875, Length=459, Percent_Identity=27.8867102396514, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24582497, Length=288, Percent_Identity=29.1666666666667, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI20129315, Length=288, Percent_Identity=29.1666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=30.3030303030303, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47830; Mature: 47699

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVAGKVLAL
CCEEEEECCCCCCCHHHEEEEEEEEHHCCHHHCCCCHHHHHHCCCCEECCCCCCCHHEEC
GGRIGEMMAVGSELIRVEVEGNGNLKPGTKARDAEADATSRPAAVDTPAKSSKVTEAAEA
CCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HDASKAARHTAERAPAEPRRTEHAAAPRAALAPGERPLASPAVRQRAWDMGIELRYVRGT
HHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECC
GEAGRILHADLDAYARTGGTAAGGAQARGYDERNDETEVPVIGLRRAIARKMQEAKRRIP
CCCCCEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
HFSYVEEIDVTELESLRSELNRRHGDTRGRLTPLPLLIRAMVIALRDFPQINARYDDEAG
CCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VVTRYGAVHMGVATQTDSGLTVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGS
EEEECCEEEECEEEECCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TITISSLGALGGIMSTPVINHPEVGIVGVNRIVERPMIRDGAIVARKLMNLSSSFDHRVV
EEEEHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
DGADAAEFIQAVRGLLERPALLFVE
CCCCHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
GIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVAGKVLAL
CEEEEECCCCCCCHHHEEEEEEEEHHCCHHHCCCCHHHHHHCCCCEECCCCCCCHHEEC
GGRIGEMMAVGSELIRVEVEGNGNLKPGTKARDAEADATSRPAAVDTPAKSSKVTEAAEA
CCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HDASKAARHTAERAPAEPRRTEHAAAPRAALAPGERPLASPAVRQRAWDMGIELRYVRGT
HHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECC
GEAGRILHADLDAYARTGGTAAGGAQARGYDERNDETEVPVIGLRRAIARKMQEAKRRIP
CCCCCEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
HFSYVEEIDVTELESLRSELNRRHGDTRGRLTPLPLLIRAMVIALRDFPQINARYDDEAG
CCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VVTRYGAVHMGVATQTDSGLTVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGS
EEEECCEEEECEEEECCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TITISSLGALGGIMSTPVINHPEVGIVGVNRIVERPMIRDGAIVARKLMNLSSSFDHRVV
EEEEHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
DGADAAEFIQAVRGLLERPALLFVE
CCCCHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]