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The map label for this gene is lpdV [H]

Identifier: 115351179

GI number: 115351179

Start: 1234739

End: 1236130

Strand: Direct

Name: lpdV [H]

Synonym: Bamb_1126

Alternate gene names: 115351179

Gene position: 1234739-1236130 (Clockwise)

Preceding gene: 115351178

Following gene: 115351180

Centisome position: 34.72

GC content: 71.26

Gene sequence:

>1392_bases
ATGCCGAACGAACACACCACGCTGCTCGTGATCGGCGGCGGGCCGGGCGGTTACGTCGCCGCGATTCGCGCGGGGCAGCT
CGGCATTCCGACGGTGCTCGTCGAGCGCGAGCGGCTCGGCGGCACGTGCCTGAACATCGGCTGCATTCCGTCGAAGGCGC
TGATCCATGTCGCCGACGCGTTCGAGCAGGCGTGCGCGCAGGCCGGCGAAGGCATGCTCGGGATTCGCGTGCGCGCGCCG
GAGATCGACATCGCGAAAAGCGTCGCATGGAAGGACGGGATCGTCGAGCGGCTCACGCGCGGCGTCGGCGCGCTGCTGAA
GAAGCACGGCGTGCGCGTGCTGCAGGGCGACGCGCGCGTCGTCGACGGCAAGACGGTCGATATCGTCGCCGGTGATCACA
CGACGCGCATCGCATGCGAACACCTGTTGCTCGCGACCGGTTCGGAACCGGTCGCGCTGCCGTCGATGCCGTTCGGCGGG
CACGTCGTATCGTCGACCGACGCACTGTCGCCCACGTCGTTGCCGACGCGGCTGGTCGTCGTCGGCGCCGGATACATCGG
GCTCGAACTCGGGACCGTCTATCGCAAGCTCGGCGTCGACGTCAGCATCGTCGAAGCGGCGGAGCGCGTGCTGCCCGCAT
ACGACGCGGAACTCGCGAAGCCGGTCGCCGATTCGCTCGCGCAGCTCGGCGTCGGCTTGTGGCTGGGTCACACGGTGCTT
GGTCTCGCGAGCGACGGCGCGGTGCGCGTGCAGGCACCCGACGGCGCGCAGAAGACGCTGCCGGCCGATCGCGTGCTGGT
CGCGGTCGGCCGCCGGCCGCGCGTCGACGGCTTCGGGCTCGAAGCGTTGCCGCTCGATCGCAACGGCCGTGCGCTGCGCA
TCGACGACGCATGCCGGACCTCGATGCGCAACGTGTGGGCGATCGGCGACGTCGCAGGCGAGCCGATGCTCGCGCATCGC
GCGATGGCGCAGGGCGAGATGGTGGCCGAGCTGATCGCGGGGCGGCGCCGCAAGTTCACGCCCGCGTCGATCCCGGCCGT
GTGCTTCACCGATCCCGAGGTCGTCACGTCCGGCTGGTCGCCCGACGACGCGAAGGCGGCCGGCGTCGACTGCATCAGCG
CGTCGTTCCCGTTCGCGGCGAACGGGCGCGCGATGACGCTGCACGCGACCGACGGTTTCGTGCGCGTCGTCGCGCGGCGC
GACACGCATCTGATCGTCGGCTGGCAGGCGGTCGGGCGCGGCGTGTCGGAACTGGCCGCGGCGTTCTCGCAGTCGCTCGA
GATGGGCGCGCGGCTCGAGGACATCGGCGGCACGATCCACGCGCATCCGACGCTCGGCGAAGCGGTGCAGGAGGCGGCAT
TGCGCGCGCTGGGACACGCGTTGCACGTGTGA

Upstream 100 bases:

>100_bases
TCGACCATCGCGTCGTCGACGGCGCGGACGCGGCCGAATTCATCCAGGCCGTGCGCGGGTTGCTCGAGCGCCCGGCGCTG
CTGTTCGTGGAGTGAGTGCG

Downstream 100 bases:

>100_bases
ACGCACGGCGGTGTCCGTCGCCATCCGTCGCCGGACTAACCCCGGGTGCGGCGCCGATCCGGACGACCGGTGCGATCGCG
TTCGAGCATGCGCGGTTCGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERERLGGTCLNIGCIPSKALIHVADAFEQACAQAGEGMLGIRVRAP
EIDIAKSVAWKDGIVERLTRGVGALLKKHGVRVLQGDARVVDGKTVDIVAGDHTTRIACEHLLLATGSEPVALPSMPFGG
HVVSSTDALSPTSLPTRLVVVGAGYIGLELGTVYRKLGVDVSIVEAAERVLPAYDAELAKPVADSLAQLGVGLWLGHTVL
GLASDGAVRVQAPDGAQKTLPADRVLVAVGRRPRVDGFGLEALPLDRNGRALRIDDACRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGRRRKFTPASIPAVCFTDPEVVTSGWSPDDAKAAGVDCISASFPFAANGRAMTLHATDGFVRVVARR
DTHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHV

Sequences:

>Translated_463_residues
MPNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERERLGGTCLNIGCIPSKALIHVADAFEQACAQAGEGMLGIRVRAP
EIDIAKSVAWKDGIVERLTRGVGALLKKHGVRVLQGDARVVDGKTVDIVAGDHTTRIACEHLLLATGSEPVALPSMPFGG
HVVSSTDALSPTSLPTRLVVVGAGYIGLELGTVYRKLGVDVSIVEAAERVLPAYDAELAKPVADSLAQLGVGLWLGHTVL
GLASDGAVRVQAPDGAQKTLPADRVLVAVGRRPRVDGFGLEALPLDRNGRALRIDDACRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGRRRKFTPASIPAVCFTDPEVVTSGWSPDDAKAAGVDCISASFPFAANGRAMTLHATDGFVRVVARR
DTHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHV
>Mature_462_residues
PNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERERLGGTCLNIGCIPSKALIHVADAFEQACAQAGEGMLGIRVRAPE
IDIAKSVAWKDGIVERLTRGVGALLKKHGVRVLQGDARVVDGKTVDIVAGDHTTRIACEHLLLATGSEPVALPSMPFGGH
VVSSTDALSPTSLPTRLVVVGAGYIGLELGTVYRKLGVDVSIVEAAERVLPAYDAELAKPVADSLAQLGVGLWLGHTVLG
LASDGAVRVQAPDGAQKTLPADRVLVAVGRRPRVDGFGLEALPLDRNGRALRIDDACRTSMRNVWAIGDVAGEPMLAHRA
MAQGEMVAELIAGRRRKFTPASIPAVCFTDPEVVTSGWSPDDAKAAGVDCISASFPFAANGRAMTLHATDGFVRVVARRD
THLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=38.8646288209607, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=28.3261802575107, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI22035672, Length=462, Percent_Identity=27.9220779220779, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI33519430, Length=444, Percent_Identity=24.5495495495495, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI33519428, Length=444, Percent_Identity=24.5495495495495, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI33519426, Length=444, Percent_Identity=24.5495495495495, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI148277065, Length=444, Percent_Identity=24.5495495495495, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI148277071, Length=444, Percent_Identity=24.5495495495495, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI291045266, Length=433, Percent_Identity=24.7113163972286, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=24.4131455399061, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786307, Length=456, Percent_Identity=38.1578947368421, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=28.0092592592593, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=27.6008492569002, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=25.4424778761062, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=39.5196506550218, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71983429, Length=437, Percent_Identity=26.0869565217391, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=437, Percent_Identity=26.0869565217391, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17557007, Length=467, Percent_Identity=23.7687366167024, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=453, Percent_Identity=23.841059602649, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=37.5, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=28.0172413793103, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=26.3829787234043, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=39.0829694323144, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI17737741, Length=481, Percent_Identity=25.987525987526, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=26.7223382045929, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=26.7223382045929, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640551, Length=479, Percent_Identity=26.7223382045929, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48279; Mature: 48148

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERERLGGTCLNIGCIPSKALIHVADA
CCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEHHHCCCEEEEECCCCCHHHHHHHHH
FEQACAQAGEGMLGIRVRAPEIDIAKSVAWKDGIVERLTRGVGALLKKHGVRVLQGDARV
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
VDGKTVDIVAGDHTTRIACEHLLLATGSEPVALPSMPFGGHVVSSTDALSPTSLPTRLVV
ECCCEEEEEECCCCHHHHHHHHHEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEE
VGAGYIGLELGTVYRKLGVDVSIVEAAERVLPAYDAELAKPVADSLAQLGVGLWLGHTVL
EECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLASDGAVRVQAPDGAQKTLPADRVLVAVGRRPRVDGFGLEALPLDRNGRALRIDDACRT
EECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEECHHHHH
SMRNVWAIGDVAGEPMLAHRAMAQGEMVAELIAGRRRKFTPASIPAVCFTDPEVVTSGWS
HHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHEECCCC
PDDAKAAGVDCISASFPFAANGRAMTLHATDGFVRVVARRDTHLIVGWQAVGRGVSELAA
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEHHHHCCCHHHHHH
AFSQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHV
HHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERERLGGTCLNIGCIPSKALIHVADA
CCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEHHHCCCEEEEECCCCCHHHHHHHHH
FEQACAQAGEGMLGIRVRAPEIDIAKSVAWKDGIVERLTRGVGALLKKHGVRVLQGDARV
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
VDGKTVDIVAGDHTTRIACEHLLLATGSEPVALPSMPFGGHVVSSTDALSPTSLPTRLVV
ECCCEEEEEECCCCHHHHHHHHHEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEE
VGAGYIGLELGTVYRKLGVDVSIVEAAERVLPAYDAELAKPVADSLAQLGVGLWLGHTVL
EECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLASDGAVRVQAPDGAQKTLPADRVLVAVGRRPRVDGFGLEALPLDRNGRALRIDDACRT
EECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEECHHHHH
SMRNVWAIGDVAGEPMLAHRAMAQGEMVAELIAGRRRKFTPASIPAVCFTDPEVVTSGWS
HHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHEECCCC
PDDAKAAGVDCISASFPFAANGRAMTLHATDGFVRVVARRDTHLIVGWQAVGRGVSELAA
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEHHHHCCCHHHHHH
AFSQSLEMGARLEDIGGTIHAHPTLGEAVQEAALRALGHALHV
HHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]