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Identifier: 11499242

GI number: 11499242

Start: 1482089

End: 1484821

Strand: Reverse

Name: Not Available

Synonym: AF1652

Alternate gene names: 11499242

Gene position: 1484821-1482089 (Counterclockwise)

Preceding gene: 11499243

Following gene: 11499232

Centisome position: 68.16

GC content: 50.02

Gene sequence:

>2733_bases
ATGGACAACTTCGTGCCGAGTTACATTAAGCTGAGGGTTAATCCCGACACGGTGAACTACGTCTGGGCTGCGCCGCCTTA
CGGTGGAACAAGGACCCTTGAAATGGACGTTGACAGGCCCTTCACGCTTAAGTTCGACATTATAGGGTATGTGAGAGGGA
GCTTTGTTGTTAAGCTTGAGGCGAACGGGGAAACCAAGGAGCTGCTCTCTCTGTCTTCTGCAACCTACAACAGCTGGAAG
AGCATACAGTTAAGCTCCAGCACTGCGGGAAGGCTAATTTTTGAGTCGTACGGTGACAACTATGTCTGGATAGACAACAT
AGCAATACCCGAAATCGGCTTTTACGATGACGTTGAGACCGGCAACGCTGGCTGGACTGCAAATGGCTGGTTCATCGTAA
TGCACACGTTGTCCAAAGAAATTCCCGAAAGCTTCGAATTCCAGCTCATCTCACCGTCAGGGACTGCAGTAAAGCCTCAG
TGCACCGTCACAAGTTCTTACTTCGACTGCCAGTATTCACCATCGACTGTCCTCGAAAAGGGCAACTGGACCATCAGGCT
CACAAATTCGGGAGACAGAAAGGTTGAGGCAGACGTTACAGCCTACGTGGTTTACCCGTCCAACGGACACGACATACTGA
GCGAGGCTGTGAACGACATTGTGAGAAGGGGAGTTGTGGCGGTAATCGCTGCAGGGAACTCGGGAGAGCTTGGGGAAAGG
AGCATAGCCTCTCCCGGCTCGGCAGAGAACGCAATAACTGTTGGAGCTGTGGACAAAGCCGGCAAAGTTGCTTACTTCAG
CAGCCGCGGGCCTGTTGGCTACAATCCAGAGTACATTAAGCCCGACGTCGTCGCTCCGGGTGTGGCGGTTGTTTCAACGT
GGAAGGATGGGGGCTACGCTTCCCTTTCAGGCACGTCAATGGCAACACCGCATGTTTCGGGAATAGCTGCGCTACTGCTT
CAGGCAAATACCGATTTTACGCCAGAAGACGTTAAGAGGGCAATTGAAGAATCAGCAACGGACATTGAGATTGCTGGGAA
GGACGTTAAGTCGGGCAGCGGTATCGTTGACGCTTACAGGGCCTTTGCAGCAGCTATGAACATGCAGGTTTCAAAGAATC
AGCCACCAGTGGTTCTCTTCGAAATTACTCCCGAAAAGCCGGTGGCAGGAGAGCCGGTATTCTTCAAATCCCTATCCTAC
GACCCGGATGGTAAAGTTGTCTGGGTTAAGTGGGAGTTCGGCGATGGAACTTCGTGGGTAGCTCCGGGAATCAACCACGT
TTACGAGAAGAGTGGGGAGTATCAGGTCAAAATCACCGCATGCGACGACAAGGGGTTAAAGAACTCAACAACCACAATCC
TGAGAGTTTATGAGCCTATGGTTACCGTTGAGGGCTACGTTCTCAACTCCCTCGGAAAGCCTGTTGATGCGGTTGTTGAA
GCGGGCTCAGAAATGACAAAGACGGATGATGACGGTTATTTCACGTTGATGGTTGAAAAGGGTGCTGTTGTAACAGTTTC
TGCCGAGGGCTATGCTGAGCACACCTTCATAGCGGATGGCGACATGACCGTCAATATAACACTCAGGGACATCAACACAC
CCGTCATTGAGCTTCCGGCCCTTCCAGAGTACATAAACTACACAGAGCTAAGCTTAGCTGTCAGCGCCTATGATGCGGAA
ACTGCGGTGGATGAGGTGATTTACCTGCTGGATGGTGTTGAAACAGCCCCGGAGCTCACCCTCACGGAAGGAAAACATGA
GGTCAAAGTTGTCGCCATCGACCATGACGGAAACAAGGCTGAAGAAACCGTTCTGATTACAGTTGATATCACCAAGCCTG
AAATAACTGTAGTTGAGGAGGGGTGGAGCGATAATGGCTACAAGGTCGTTCTCAGAACCTCGGAAGAAGTTGAGCTGAGA
GTAGATGAAGGTGACTGCAGTGTAAGTGGAGACGGAATTAGCTGGGATGTTGTGGTCAAATCAACCCATGCAAAGCTCAC
CGCTATTGACAAGGCCGGAAATGCTAACAGCATTGAAATAAACCTACCTGAAATTCAGATAGAGCTGCTTGAGCCGAGTG
GCGTAACAAACAAGGCAGAATTCAAATTTGTGCTGCCCGTTGAAGCTGCCTTTACGCTCTACGTTGACGGCAACGCCGTG
TATGAGGGCTATGGTTCAGGGGAAATTACTGTAAACGCAAACATTGCTGACGGAGACCACACGTGGTGGGTTGAAGCTTT
GAATGCGGCAAGTGAAAGGCTAACCTTTACTCTCGATACAAAGCCACCTGAAGTTCTCAGTGTAGAAAAGAATAGCGACA
CGCTCATCGTAAAACCGTCGGAAGAGTTGAAGTGGGCCAGGGCCAACGGTATTGAGATGCAGAGAGAAGGAGACGTCTGG
AAGTGCAGCATCGCAGATTCAACCATTAAGATAACCATGGAAGACCTTGCGGGCAATGTTGGAAGCTACACCTACGAAGA
GAAAGCGGTTGTTGCCGACTTCACCTACACGCCAGAAGAGCCAAAGGCAGGTCAAAGAATTACTTTCAAGGCCTACGTTG
AAGGAATAAACTATAAACAGATCATCTGGGTGTTCAGCAACGGCCGAATGAGGATGGGGGCAACAACAACAATGTCCTTC
GGCTCACCGGGGGAGTACACGGTGAAAATGGCCGTGATTGACTATAGCTACAAGGTTGTAAAAGTGGTTGTGAAAAATAT
TGTTGTTTCTTAA

Upstream 100 bases:

>100_bases
TGCCCGGATTGGGGGGAGCTACGGAAAAGTTCTCAACTCAGGAGAGAGCTACGAAATTAATCTTCCGGTTTATTCGAGCC
TCGGAGAAGTTTACGATGAC

Downstream 100 bases:

>100_bases
CCTTTTATTTTTTCGAGAATCTCCTCAAACGATGTCGGCTCCCCCTCAGCCACCTCAATATTGGCCTTGAACCTCTTGAA
ACCATCAAAGCCTGTCAGGT

Product: prepro-subtilisin sendai, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 910; Mature: 910

Protein sequence:

>910_residues
MDNFVPSYIKLRVNPDTVNYVWAAPPYGGTRTLEMDVDRPFTLKFDIIGYVRGSFVVKLEANGETKELLSLSSATYNSWK
SIQLSSSTAGRLIFESYGDNYVWIDNIAIPEIGFYDDVETGNAGWTANGWFIVMHTLSKEIPESFEFQLISPSGTAVKPQ
CTVTSSYFDCQYSPSTVLEKGNWTIRLTNSGDRKVEADVTAYVVYPSNGHDILSEAVNDIVRRGVVAVIAAGNSGELGER
SIASPGSAENAITVGAVDKAGKVAYFSSRGPVGYNPEYIKPDVVAPGVAVVSTWKDGGYASLSGTSMATPHVSGIAALLL
QANTDFTPEDVKRAIEESATDIEIAGKDVKSGSGIVDAYRAFAAAMNMQVSKNQPPVVLFEITPEKPVAGEPVFFKSLSY
DPDGKVVWVKWEFGDGTSWVAPGINHVYEKSGEYQVKITACDDKGLKNSTTTILRVYEPMVTVEGYVLNSLGKPVDAVVE
AGSEMTKTDDDGYFTLMVEKGAVVTVSAEGYAEHTFIADGDMTVNITLRDINTPVIELPALPEYINYTELSLAVSAYDAE
TAVDEVIYLLDGVETAPELTLTEGKHEVKVVAIDHDGNKAEETVLITVDITKPEITVVEEGWSDNGYKVVLRTSEEVELR
VDEGDCSVSGDGISWDVVVKSTHAKLTAIDKAGNANSIEINLPEIQIELLEPSGVTNKAEFKFVLPVEAAFTLYVDGNAV
YEGYGSGEITVNANIADGDHTWWVEALNAASERLTFTLDTKPPEVLSVEKNSDTLIVKPSEELKWARANGIEMQREGDVW
KCSIADSTIKITMEDLAGNVGSYTYEEKAVVADFTYTPEEPKAGQRITFKAYVEGINYKQIIWVFSNGRMRMGATTTMSF
GSPGEYTVKMAVIDYSYKVVKVVVKNIVVS

Sequences:

>Translated_910_residues
MDNFVPSYIKLRVNPDTVNYVWAAPPYGGTRTLEMDVDRPFTLKFDIIGYVRGSFVVKLEANGETKELLSLSSATYNSWK
SIQLSSSTAGRLIFESYGDNYVWIDNIAIPEIGFYDDVETGNAGWTANGWFIVMHTLSKEIPESFEFQLISPSGTAVKPQ
CTVTSSYFDCQYSPSTVLEKGNWTIRLTNSGDRKVEADVTAYVVYPSNGHDILSEAVNDIVRRGVVAVIAAGNSGELGER
SIASPGSAENAITVGAVDKAGKVAYFSSRGPVGYNPEYIKPDVVAPGVAVVSTWKDGGYASLSGTSMATPHVSGIAALLL
QANTDFTPEDVKRAIEESATDIEIAGKDVKSGSGIVDAYRAFAAAMNMQVSKNQPPVVLFEITPEKPVAGEPVFFKSLSY
DPDGKVVWVKWEFGDGTSWVAPGINHVYEKSGEYQVKITACDDKGLKNSTTTILRVYEPMVTVEGYVLNSLGKPVDAVVE
AGSEMTKTDDDGYFTLMVEKGAVVTVSAEGYAEHTFIADGDMTVNITLRDINTPVIELPALPEYINYTELSLAVSAYDAE
TAVDEVIYLLDGVETAPELTLTEGKHEVKVVAIDHDGNKAEETVLITVDITKPEITVVEEGWSDNGYKVVLRTSEEVELR
VDEGDCSVSGDGISWDVVVKSTHAKLTAIDKAGNANSIEINLPEIQIELLEPSGVTNKAEFKFVLPVEAAFTLYVDGNAV
YEGYGSGEITVNANIADGDHTWWVEALNAASERLTFTLDTKPPEVLSVEKNSDTLIVKPSEELKWARANGIEMQREGDVW
KCSIADSTIKITMEDLAGNVGSYTYEEKAVVADFTYTPEEPKAGQRITFKAYVEGINYKQIIWVFSNGRMRMGATTTMSF
GSPGEYTVKMAVIDYSYKVVKVVVKNIVVS
>Mature_910_residues
MDNFVPSYIKLRVNPDTVNYVWAAPPYGGTRTLEMDVDRPFTLKFDIIGYVRGSFVVKLEANGETKELLSLSSATYNSWK
SIQLSSSTAGRLIFESYGDNYVWIDNIAIPEIGFYDDVETGNAGWTANGWFIVMHTLSKEIPESFEFQLISPSGTAVKPQ
CTVTSSYFDCQYSPSTVLEKGNWTIRLTNSGDRKVEADVTAYVVYPSNGHDILSEAVNDIVRRGVVAVIAAGNSGELGER
SIASPGSAENAITVGAVDKAGKVAYFSSRGPVGYNPEYIKPDVVAPGVAVVSTWKDGGYASLSGTSMATPHVSGIAALLL
QANTDFTPEDVKRAIEESATDIEIAGKDVKSGSGIVDAYRAFAAAMNMQVSKNQPPVVLFEITPEKPVAGEPVFFKSLSY
DPDGKVVWVKWEFGDGTSWVAPGINHVYEKSGEYQVKITACDDKGLKNSTTTILRVYEPMVTVEGYVLNSLGKPVDAVVE
AGSEMTKTDDDGYFTLMVEKGAVVTVSAEGYAEHTFIADGDMTVNITLRDINTPVIELPALPEYINYTELSLAVSAYDAE
TAVDEVIYLLDGVETAPELTLTEGKHEVKVVAIDHDGNKAEETVLITVDITKPEITVVEEGWSDNGYKVVLRTSEEVELR
VDEGDCSVSGDGISWDVVVKSTHAKLTAIDKAGNANSIEINLPEIQIELLEPSGVTNKAEFKFVLPVEAAFTLYVDGNAV
YEGYGSGEITVNANIADGDHTWWVEALNAASERLTFTLDTKPPEVLSVEKNSDTLIVKPSEELKWARANGIEMQREGDVW
KCSIADSTIKITMEDLAGNVGSYTYEEKAVVADFTYTPEEPKAGQRITFKAYVEGINYKQIIWVFSNGRMRMGATTTMSF
GSPGEYTVKMAVIDYSYKVVKVVVKNIVVS

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320775, Length=145, Percent_Identity=38.6206896551724, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324576, Length=107, Percent_Identity=43.9252336448598, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319893, Length=132, Percent_Identity=39.3939393939394, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 98999; Mature: 98999

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50093 PKD ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFVPSYIKLRVNPDTVNYVWAAPPYGGTRTLEMDVDRPFTLKFDIIGYVRGSFVVKLE
CCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEEEEECCEEEEEEE
ANGETKELLSLSSATYNSWKSIQLSSSTAGRLIFESYGDNYVWIDNIAIPEIGFYDDVET
CCCCHHHHHEECCCCCCCEEEEEECCCCCCEEEEEECCCCEEEEECEECCCCCCCCCCCC
GNAGWTANGWFIVMHTLSKEIPESFEFQLISPSGTAVKPQCTVTSSYFDCQYSPSTVLEK
CCCCEECCCEEEEEEEHHHHCCCCCEEEEECCCCCEECCEEEEECCEEEEECCCHHEEEC
GNWTIRLTNSGDRKVEADVTAYVVYPSNGHDILSEAVNDIVRRGVVAVIAAGNSGELGER
CCEEEEEECCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
SIASPGSAENAITVGAVDKAGKVAYFSSRGPVGYNPEYIKPDVVAPGVAVVSTWKDGGYA
CCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEE
SLSGTSMATPHVSGIAALLLQANTDFTPEDVKRAIEESATDIEIAGKDVKSGSGIVDAYR
EECCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
AFAAAMNMQVSKNQPPVVLFEITPEKPVAGEPVFFKSLSYDPDGKVVWVKWEFGDGTSWV
HHHHHHCEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCC
APGINHVYEKSGEYQVKITACDDKGLKNSTTTILRVYEPMVTVEGYVLNSLGKPVDAVVE
CCCHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEEECCEEEECCEEEHHCCCCHHHHHH
AGSEMTKTDDDGYFTLMVEKGAVVTVSAEGYAEHTFIADGDMTVNITLRDINTPVIELPA
CCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCEEECCC
LPEYINYTELSLAVSAYDAETAVDEVIYLLDGVETAPELTLTEGKHEVKVVAIDHDGNKA
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCC
EETVLITVDITKPEITVVEEGWSDNGYKVVLRTSEEVELRVDEGDCSVSGDGISWDVVVK
CEEEEEEEEECCCEEEEEECCCCCCCEEEEEEECCEEEEEEECCCCEECCCCEEEEEEEE
STHAKLTAIDKAGNANSIEINLPEIQIELLEPSGVTNKAEFKFVLPVEAAFTLYVDGNAV
ECCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCCCCEEEEEEEECEEEEEEECCCEE
YEGYGSGEITVNANIADGDHTWWVEALNAASERLTFTLDTKPPEVLSVEKNSDTLIVKPS
EECCCCCEEEEEEEECCCCCEEHHHHHHCCCCEEEEEECCCCCCEEEEECCCCEEEECCH
EELKWARANGIEMQREGDVWKCSIADSTIKITMEDLAGNVGSYTYEEKAVVADFTYTPEE
HHHCEEECCCEEEEECCCEEEEEECCCEEEEEEHHHCCCCCCEEECCCEEEEEEEECCCC
PKAGQRITFKAYVEGINYKQIIWVFSNGRMRMGATTTMSFGSPGEYTVKMAVIDYSYKVV
CCCCCEEEEEEEECCCCEEEEEEEEECCEEEECEEEEEECCCCCCEEEEEEEEECCHHHH
KVVVKNIVVS
HHHHHHHHCC
>Mature Secondary Structure
MDNFVPSYIKLRVNPDTVNYVWAAPPYGGTRTLEMDVDRPFTLKFDIIGYVRGSFVVKLE
CCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEEEEECCEEEEEEE
ANGETKELLSLSSATYNSWKSIQLSSSTAGRLIFESYGDNYVWIDNIAIPEIGFYDDVET
CCCCHHHHHEECCCCCCCEEEEEECCCCCCEEEEEECCCCEEEEECEECCCCCCCCCCCC
GNAGWTANGWFIVMHTLSKEIPESFEFQLISPSGTAVKPQCTVTSSYFDCQYSPSTVLEK
CCCCEECCCEEEEEEEHHHHCCCCCEEEEECCCCCEECCEEEEECCEEEEECCCHHEEEC
GNWTIRLTNSGDRKVEADVTAYVVYPSNGHDILSEAVNDIVRRGVVAVIAAGNSGELGER
CCEEEEEECCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
SIASPGSAENAITVGAVDKAGKVAYFSSRGPVGYNPEYIKPDVVAPGVAVVSTWKDGGYA
CCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEE
SLSGTSMATPHVSGIAALLLQANTDFTPEDVKRAIEESATDIEIAGKDVKSGSGIVDAYR
EECCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
AFAAAMNMQVSKNQPPVVLFEITPEKPVAGEPVFFKSLSYDPDGKVVWVKWEFGDGTSWV
HHHHHHCEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCC
APGINHVYEKSGEYQVKITACDDKGLKNSTTTILRVYEPMVTVEGYVLNSLGKPVDAVVE
CCCHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEEECCEEEECCEEEHHCCCCHHHHHH
AGSEMTKTDDDGYFTLMVEKGAVVTVSAEGYAEHTFIADGDMTVNITLRDINTPVIELPA
CCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCEEECCC
LPEYINYTELSLAVSAYDAETAVDEVIYLLDGVETAPELTLTEGKHEVKVVAIDHDGNKA
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCC
EETVLITVDITKPEITVVEEGWSDNGYKVVLRTSEEVELRVDEGDCSVSGDGISWDVVVK
CEEEEEEEEECCCEEEEEECCCCCCCEEEEEEECCEEEEEEECCCCEECCCCEEEEEEEE
STHAKLTAIDKAGNANSIEINLPEIQIELLEPSGVTNKAEFKFVLPVEAAFTLYVDGNAV
ECCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCCCCEEEEEEEECEEEEEEECCCEE
YEGYGSGEITVNANIADGDHTWWVEALNAASERLTFTLDTKPPEVLSVEKNSDTLIVKPS
EECCCCCEEEEEEEECCCCCEEHHHHHHCCCCEEEEEECCCCCCEEEEECCCCEEEECCH
EELKWARANGIEMQREGDVWKCSIADSTIKITMEDLAGNVGSYTYEEKAVVADFTYTPEE
HHHCEEECCCEEEEECCCEEEEEECCCEEEEEEHHHCCCCCCEEECCCEEEEEEEECCCC
PKAGQRITFKAYVEGINYKQIIWVFSNGRMRMGATTTMSFGSPGEYTVKMAVIDYSYKVV
CCCCCEEEEEEEECCCCEEEEEEEEECCEEEECEEEEEECCCCCCEEEEEEEEECCHHHH
KVVVKNIVVS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]