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The map label for this gene is hpaA-3

Identifier: 11498632

GI number: 11498632

Start: 928737

End: 930179

Strand: Reverse

Name: hpaA-3

Synonym: AF1027

Alternate gene names: 11498632

Gene position: 930179-928737 (Counterclockwise)

Preceding gene: 11498633

Following gene: 11498630

Centisome position: 42.7

GC content: 51.28

Gene sequence:

>1443_bases
ATGAGAACATCGGAGGAATACAGGCAGGATTTGTATAAGCTGAAGCCGAATGTTTATGTTAGAGGAAAGAAAGTGAGAAG
GGATTCACCCGAAGTGAGTGGAGGAATCAATGTCATTTCCAAAACATTCGACCTCGTAGAGCATCCAGACTACAAGGATT
TGCTGACCACAAAGTCCCACATTTCCGGAAAGAGAATCAACCGCTTCACCCACATAAATCAATCTGCGGAGGATTTGCTA
AAAAAGCAGCTAATGACCAGAAAGCTCTGCAGGCTCGTTGGGGGTTGCATCCAGCGCTGTATGGGTTGTGATGCTATAAA
CGGCCTCTCCGTCGCAACCTTTGCAGCAGACACGGAGCACGGAACCGACTATCACAAAAGGTTTCTTGAGTACGTAAAGG
AGTTTCAGGAGAGGGATCTTGTTGCTGCCTGCGCCCAGACGGACGTGAAGGGGGACAGAAGCAAGAGACCCCACCAGCAG
GACGACCCGGACATGTACGTGAGAGTTGTGGAGAGGAGGAGCGATGGAATTGTTGTGAGGGGAGCGAAGAACTGCATAAC
CATGGCTGCTGTGGCGGATGAGATAATTGTTGTACCAACGAGAGCGATGACCCCTGAGGATAAGGACTACAGCGTGGCCT
TCGCAGTTCCAGCAGATACTGAAGGCGTGAAGATTGTCGCAAGGACGAGCAAACTGAGAGCACCTGAAGGTCTGAAGATG
CCGCAGGGGGAGATAGGGGACGACGAGAACATGATAATCTTCGACGATGTGTTTGTGCCGTGGGATAGAGTGTTTTTATG
CGGAGAAAACAAGTACGCTACGCTTGCAGCACACCTCTTTGCCCTCTTCCACAGACACAGCTACACGGGCTGCAAGCCCG
CCACCACGGACATGATAATGGGCTTTGGCGCTTTAATTGCAGAGTACAATGGGGTTTACGAGAAGCACAACATTAGAGAA
AAGCTGGTTGAGCTTGCTGCAACTGCAGAACTTGTGTTTGCTGCTGGAGTAGCAGCAGCACACTACGGTCAGAAATCCCC
TGCAGGAACCTTTATTCCCAATGAAGTTTACGCCAACGTTGGAAGGAGGCATGCGGGCCTGAACTACTACAAGGAGCTTG
AAATTCTTGCAGAGCTTTCTGGTGGGTTGCCTGCTGCACTGCCCTTTGCCGAAGACTTCCTGAGCGAGGAGGTGGGCCCG
TACATCAGAAAGTACATCAAGAGAAGGGCTGACGTTCCGGCGGAGCATGTGTACCGCTGCCTGTTCGGAATATCCAACAT
CCTCTGCTCCTCCCTCGGAGGTGTTCAGGCGGTTGCAGGAGTTCACGGTGGAGGCTCGCCGGTGATGGAAGAAATTGCAA
TATGGAGGAGCTACGACTTCGAGAAGAAAAAGGACATCGCAAAGTACCTTTCTGGCATAAGCGAGGAGCTTCCGAGAGAT
TGA

Upstream 100 bases:

>100_bases
AACCGCCTGCGTCGGCGGCGGACAGGGCGCGGCGGTTGTAATTGAGAGAGTTTAATTTTTTTAAAAAATATTTTTAAAAA
TTAATTGTGTTGGTGATGTT

Downstream 100 bases:

>100_bases
TTTTTTGAATTTTTTACATCATTCAAAAAACAAAAGGGAAAATTTACTGGAGGGGATGCTTCCTCACCGCTCCAAGCCTC
GAGGGCACTTTGCCCAGCAA

Product: 4-hydroxyphenylacetate-3-hydroxylase (hpaA-3)

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MRTSEEYRQDLYKLKPNVYVRGKKVRRDSPEVSGGINVISKTFDLVEHPDYKDLLTTKSHISGKRINRFTHINQSAEDLL
KKQLMTRKLCRLVGGCIQRCMGCDAINGLSVATFAADTEHGTDYHKRFLEYVKEFQERDLVAACAQTDVKGDRSKRPHQQ
DDPDMYVRVVERRSDGIVVRGAKNCITMAAVADEIIVVPTRAMTPEDKDYSVAFAVPADTEGVKIVARTSKLRAPEGLKM
PQGEIGDDENMIIFDDVFVPWDRVFLCGENKYATLAAHLFALFHRHSYTGCKPATTDMIMGFGALIAEYNGVYEKHNIRE
KLVELAATAELVFAAGVAAAHYGQKSPAGTFIPNEVYANVGRRHAGLNYYKELEILAELSGGLPAALPFAEDFLSEEVGP
YIRKYIKRRADVPAEHVYRCLFGISNILCSSLGGVQAVAGVHGGGSPVMEEIAIWRSYDFEKKKDIAKYLSGISEELPRD

Sequences:

>Translated_480_residues
MRTSEEYRQDLYKLKPNVYVRGKKVRRDSPEVSGGINVISKTFDLVEHPDYKDLLTTKSHISGKRINRFTHINQSAEDLL
KKQLMTRKLCRLVGGCIQRCMGCDAINGLSVATFAADTEHGTDYHKRFLEYVKEFQERDLVAACAQTDVKGDRSKRPHQQ
DDPDMYVRVVERRSDGIVVRGAKNCITMAAVADEIIVVPTRAMTPEDKDYSVAFAVPADTEGVKIVARTSKLRAPEGLKM
PQGEIGDDENMIIFDDVFVPWDRVFLCGENKYATLAAHLFALFHRHSYTGCKPATTDMIMGFGALIAEYNGVYEKHNIRE
KLVELAATAELVFAAGVAAAHYGQKSPAGTFIPNEVYANVGRRHAGLNYYKELEILAELSGGLPAALPFAEDFLSEEVGP
YIRKYIKRRADVPAEHVYRCLFGISNILCSSLGGVQAVAGVHGGGSPVMEEIAIWRSYDFEKKKDIAKYLSGISEELPRD
>Mature_480_residues
MRTSEEYRQDLYKLKPNVYVRGKKVRRDSPEVSGGINVISKTFDLVEHPDYKDLLTTKSHISGKRINRFTHINQSAEDLL
KKQLMTRKLCRLVGGCIQRCMGCDAINGLSVATFAADTEHGTDYHKRFLEYVKEFQERDLVAACAQTDVKGDRSKRPHQQ
DDPDMYVRVVERRSDGIVVRGAKNCITMAAVADEIIVVPTRAMTPEDKDYSVAFAVPADTEGVKIVARTSKLRAPEGLKM
PQGEIGDDENMIIFDDVFVPWDRVFLCGENKYATLAAHLFALFHRHSYTGCKPATTDMIMGFGALIAEYNGVYEKHNIRE
KLVELAATAELVFAAGVAAAHYGQKSPAGTFIPNEVYANVGRRHAGLNYYKELEILAELSGGLPAALPFAEDFLSEEVGP
YIRKYIKRRADVPAEHVYRCLFGISNILCSSLGGVQAVAGVHGGGSPVMEEIAIWRSYDFEKKKDIAKYLSGISEELPRD

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 53499; Mature: 53499

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSEEYRQDLYKLKPNVYVRGKKVRRDSPEVSGGINVISKTFDLVEHPDYKDLLTTKSH
CCCHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ISGKRINRFTHINQSAEDLLKKQLMTRKLCRLVGGCIQRCMGCDAINGLSVATFAADTEH
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
GTDYHKRFLEYVKEFQERDLVAACAQTDVKGDRSKRPHQQDDPDMYVRVVERRSDGIVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
GAKNCITMAAVADEIIVVPTRAMTPEDKDYSVAFAVPADTEGVKIVARTSKLRAPEGLKM
CCCHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEECHHCCCCCCCCC
PQGEIGDDENMIIFDDVFVPWDRVFLCGENKYATLAAHLFALFHRHSYTGCKPATTDMIM
CCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GFGALIAEYNGVYEKHNIREKLVELAATAELVFAAGVAAAHYGQKSPAGTFIPNEVYANV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
GRRHAGLNYYKELEILAELSGGLPAALPFAEDFLSEEVGPYIRKYIKRRADVPAEHVYRC
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LFGISNILCSSLGGVQAVAGVHGGGSPVMEEIAIWRSYDFEKKKDIAKYLSGISEELPRD
HHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTSEEYRQDLYKLKPNVYVRGKKVRRDSPEVSGGINVISKTFDLVEHPDYKDLLTTKSH
CCCHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ISGKRINRFTHINQSAEDLLKKQLMTRKLCRLVGGCIQRCMGCDAINGLSVATFAADTEH
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
GTDYHKRFLEYVKEFQERDLVAACAQTDVKGDRSKRPHQQDDPDMYVRVVERRSDGIVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
GAKNCITMAAVADEIIVVPTRAMTPEDKDYSVAFAVPADTEGVKIVARTSKLRAPEGLKM
CCCHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEECHHCCCCCCCCC
PQGEIGDDENMIIFDDVFVPWDRVFLCGENKYATLAAHLFALFHRHSYTGCKPATTDMIM
CCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GFGALIAEYNGVYEKHNIREKLVELAATAELVFAAGVAAAHYGQKSPAGTFIPNEVYANV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
GRRHAGLNYYKELEILAELSGGLPAALPFAEDFLSEEVGPYIRKYIKRRADVPAEHVYRC
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LFGISNILCSSLGGVQAVAGVHGGGSPVMEEIAIWRSYDFEKKKDIAKYLSGISEELPRD
HHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]