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The map label for this gene is argH

Identifier: 11498488

GI number: 11498488

Start: 786759

End: 788210

Strand: Reverse

Name: argH

Synonym: AF0883

Alternate gene names: 11498488

Gene position: 788210-786759 (Counterclockwise)

Preceding gene: 11498490

Following gene: 11498487

Centisome position: 36.18

GC content: 48.9

Gene sequence:

>1452_bases
ATGCTGAGAGAAAGCAGGATGAAGAAAGGCATGGACCCAAAGGCTCTGAAGCTCTCCTCTTCAATCGAGCACGATAAAAA
TATCTTCCTGTACGATATTCTCGTTGACGTTGCCCACGTCCTGACTCTCAAGAAGGGAGGCTACTTAAGCGAAGAAGAAG
CAAAGGAGATAATTTTGGCTCTGAAAAAGGTAAAGGATTCTGGATTTAGGGAAGATTGGCCCTACGAAGACGTTCACGAA
GCCATTGAGGCAGAGGTGACGAAGATAACCCCTCACGGTGCAAAAATGCACACGGGAAGGAGCAGAAACGATGAGGTTGC
AACCTGCCTGAGAATGTTCGCGAGAGACCACCTGCTGAATCTCGCAGAGGCAATCCTCAACGCGCTGGATGTTTTGATTA
AGAAGGCCGAGAAGTCCCACTTTCTGATGCCCGGATTCACGCATCTCCAGTATGCGCAGCCAACGAGGCTGTCGCATCAC
CTTCTCGCTTATCACGATATGCTCTCAAGAGACTTTGAGAGGGCAATAGAGGCGTTCAGGAGAGTGAACAAATCACCTCT
CGGTTCCGCTGCCTTCGCGTCCACGGGATACAGCCTTGACAGAGTCTACGCTGCAAGGTTGCTGGGATTTGACGGAGTCG
TTGAGCACAGCGAGGATGCAGTGGCGTCGAGGGACTTCGTTATTGAGTCCATTTTTGTGGCGGCCGAGGCAATGCTTTCA
ATCAGCAGAATTGCCGAGGAAATTGTCCTGTTTTCGTCAGAATTCGGCTTCATCACCTTACCTGATGAGTTTTCGTCAAC
GTCCTCCATCATGCCTCAGAAAAAGAATCCTGACATTGCAGAACTTTTGAGAGCCAATGCGGGAAAAATTGCGGGGAATT
TAACGTCAGCAATGATGATTTACAAGGCGACACCCTTCAGCTACAACAGGGACTTTCAGGAGATGAATCCCCTGCTCTAC
GAATCTCTGAAAAGAACGCACTTGGCTGTGGAGGTTTTTGCCTCAATGATGGGTAAAATAAAGTTCAACCCTGAGATTAC
GGAAAGAAAAGCCTCAAAGGGGTTTGCCACCGCAACCGAGCTTGCAGACATGCTCGTAATGAAGTACGGCGTTCCCTTCA
GGATGGCGCATCGCATTGTTGGGAGGTTGGCTGCAAAGGAGCTGGAGAGGCCTACAGCATCCGATGTTAATTCGGCAGCT
AAAGAACTTGGTGTTGAAATAAACGTCAGAGATGAAGACGTGCTTGAAGCTCTGGACGTTGAGAAGGTCGTGGAGAACAG
GGGAAATCTGGGAGGGACGAGCAAGGCTGAGGTGGAGAGAATGATAAAAGCGAGGAAAAGCGATCTGAAAGACAGGAAAA
CTTTGTTAAGGAAGCTTAGGGGAGAGGTTAAAATGGGGCTTAAAATGCTTTACGATGAGGCCAAAAAGCTGGGGGTGGAT
ATCAATGTCTGA

Upstream 100 bases:

>100_bases
AGCTCTATCCTGACTATTCTGTACTTCATGCTCTGCCATCATCTCCAAAACATAAATACATTTGCGGTAAGAATTAATAA
CCTCTGCACAAACTGAGTGC

Downstream 100 bases:

>100_bases
AAGGCTTGAAGGCAAGAGAGTCAGAATAAAGGCAAAGGGAAAGATTTTTGAAGGCATAGTGATGCCCTCCTTTACCGGAA
ACTTTGTCCTGAAGCTTGAT

Product: argininosuccinate lyase (argH)

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLRESRMKKGMDPKALKLSSSIEHDKNIFLYDILVDVAHVLTLKKGGYLSEEEAKEIILALKKVKDSGFREDWPYEDVHE
AIEAEVTKITPHGAKMHTGRSRNDEVATCLRMFARDHLLNLAEAILNALDVLIKKAEKSHFLMPGFTHLQYAQPTRLSHH
LLAYHDMLSRDFERAIEAFRRVNKSPLGSAAFASTGYSLDRVYAARLLGFDGVVEHSEDAVASRDFVIESIFVAAEAMLS
ISRIAEEIVLFSSEFGFITLPDEFSSTSSIMPQKKNPDIAELLRANAGKIAGNLTSAMMIYKATPFSYNRDFQEMNPLLY
ESLKRTHLAVEVFASMMGKIKFNPEITERKASKGFATATELADMLVMKYGVPFRMAHRIVGRLAAKELERPTASDVNSAA
KELGVEINVRDEDVLEALDVEKVVENRGNLGGTSKAEVERMIKARKSDLKDRKTLLRKLRGEVKMGLKMLYDEAKKLGVD
INV

Sequences:

>Translated_483_residues
MLRESRMKKGMDPKALKLSSSIEHDKNIFLYDILVDVAHVLTLKKGGYLSEEEAKEIILALKKVKDSGFREDWPYEDVHE
AIEAEVTKITPHGAKMHTGRSRNDEVATCLRMFARDHLLNLAEAILNALDVLIKKAEKSHFLMPGFTHLQYAQPTRLSHH
LLAYHDMLSRDFERAIEAFRRVNKSPLGSAAFASTGYSLDRVYAARLLGFDGVVEHSEDAVASRDFVIESIFVAAEAMLS
ISRIAEEIVLFSSEFGFITLPDEFSSTSSIMPQKKNPDIAELLRANAGKIAGNLTSAMMIYKATPFSYNRDFQEMNPLLY
ESLKRTHLAVEVFASMMGKIKFNPEITERKASKGFATATELADMLVMKYGVPFRMAHRIVGRLAAKELERPTASDVNSAA
KELGVEINVRDEDVLEALDVEKVVENRGNLGGTSKAEVERMIKARKSDLKDRKTLLRKLRGEVKMGLKMLYDEAKKLGVD
INV
>Mature_483_residues
MLRESRMKKGMDPKALKLSSSIEHDKNIFLYDILVDVAHVLTLKKGGYLSEEEAKEIILALKKVKDSGFREDWPYEDVHE
AIEAEVTKITPHGAKMHTGRSRNDEVATCLRMFARDHLLNLAEAILNALDVLIKKAEKSHFLMPGFTHLQYAQPTRLSHH
LLAYHDMLSRDFERAIEAFRRVNKSPLGSAAFASTGYSLDRVYAARLLGFDGVVEHSEDAVASRDFVIESIFVAAEAMLS
ISRIAEEIVLFSSEFGFITLPDEFSSTSSIMPQKKNPDIAELLRANAGKIAGNLTSAMMIYKATPFSYNRDFQEMNPLLY
ESLKRTHLAVEVFASMMGKIKFNPEITERKASKGFATATELADMLVMKYGVPFRMAHRIVGRLAAKELERPTASDVNSAA
KELGVEINVRDEDVLEALDVEKVVENRGNLGGTSKAEVERMIKARKSDLKDRKTLLRKLRGEVKMGLKMLYDEAKKLGVD
INV

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=459, Percent_Identity=33.9869281045752, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI68303542, Length=459, Percent_Identity=33.9869281045752, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI68303547, Length=459, Percent_Identity=32.4618736383442, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI68303549, Length=459, Percent_Identity=31.5904139433551, Blast_Score=211, Evalue=8e-55,
Organism=Escherichia coli, GI1790398, Length=450, Percent_Identity=36.8888888888889, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321806, Length=465, Percent_Identity=36.1290322580645, Blast_Score=259, Evalue=8e-70,
Organism=Drosophila melanogaster, GI221473854, Length=459, Percent_Identity=33.5511982570806, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI78706858, Length=459, Percent_Identity=33.5511982570806, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_ARCFU (O29379)

Other databases:

- EMBL:   AE000782
- PIR:   C69360
- RefSeq:   NP_069716.1
- ProteinModelPortal:   O29379
- SMR:   O29379
- GeneID:   1484105
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0883
- NMPDR:   fig|224325.1.peg.875
- TIGR:   AF_0883
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- ProtClustDB:   CLSK2746644
- BioCyc:   AFUL224325:AF_0883-MONOMER
- BRENDA:   4.3.2.1
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 54202; Mature: 54202

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRESRMKKGMDPKALKLSSSIEHDKNIFLYDILVDVAHVLTLKKGGYLSEEEAKEIILA
CCCHHHHHCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LKKVKDSGFREDWPYEDVHEAIEAEVTKITPHGAKMHTGRSRNDEVATCLRMFARDHLLN
HHHHHCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAEAILNALDVLIKKAEKSHFLMPGFTHLQYAQPTRLSHHLLAYHDMLSRDFERAIEAFR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVNKSPLGSAAFASTGYSLDRVYAARLLGFDGVVEHSEDAVASRDFVIESIFVAAEAMLS
HCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ISRIAEEIVLFSSEFGFITLPDEFSSTSSIMPQKKNPDIAELLRANAGKIAGNLTSAMMI
HHHHHHHHHHHCCCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
YKATPFSYNRDFQEMNPLLYESLKRTHLAVEVFASMMGKIKFNPEITERKASKGFATATE
HCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHH
LADMLVMKYGVPFRMAHRIVGRLAAKELERPTASDVNSAAKELGVEINVRDEDVLEALDV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHH
EKVVENRGNLGGTSKAEVERMIKARKSDLKDRKTLLRKLRGEVKMGLKMLYDEAKKLGVD
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
INV
CCC
>Mature Secondary Structure
MLRESRMKKGMDPKALKLSSSIEHDKNIFLYDILVDVAHVLTLKKGGYLSEEEAKEIILA
CCCHHHHHCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LKKVKDSGFREDWPYEDVHEAIEAEVTKITPHGAKMHTGRSRNDEVATCLRMFARDHLLN
HHHHHCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAEAILNALDVLIKKAEKSHFLMPGFTHLQYAQPTRLSHHLLAYHDMLSRDFERAIEAFR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVNKSPLGSAAFASTGYSLDRVYAARLLGFDGVVEHSEDAVASRDFVIESIFVAAEAMLS
HCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ISRIAEEIVLFSSEFGFITLPDEFSSTSSIMPQKKNPDIAELLRANAGKIAGNLTSAMMI
HHHHHHHHHHHCCCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
YKATPFSYNRDFQEMNPLLYESLKRTHLAVEVFASMMGKIKFNPEITERKASKGFATATE
HCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHH
LADMLVMKYGVPFRMAHRIVGRLAAKELERPTASDVNSAAKELGVEINVRDEDVLEALDV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHH
EKVVENRGNLGGTSKAEVERMIKARKSDLKDRKTLLRKLRGEVKMGLKMLYDEAKKLGVD
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
INV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475