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The map label for this gene is hpaA-2

Identifier: 11498490

GI number: 11498490

Start: 788730

End: 790115

Strand: Reverse

Name: hpaA-2

Synonym: AF0885

Alternate gene names: 11498490

Gene position: 790115-788730 (Counterclockwise)

Preceding gene: 11498491

Following gene: 11498488

Centisome position: 36.27

GC content: 50.94

Gene sequence:

>1386_bases
ATGCTTTTAAAGCCGGAGGAATACATAGAGAGGTTGGGTGAATACAAAAGGGATATCTACGTTCTCGGAAAGAAAATTGA
GGATTTCGTAAACCATCCGAATATAAGGCCGACGGTTGAGGCAATGGCCTATACCTACAGCATAGCGGAAAAGTACGCTC
CACACTCCGAGCTTGTTGGAGAGAAGGTAAACAGGCTGAACTACGTTAACCAAACTTTTGAAGACCTGATTGCGAGATAT
GATTATCAGAGAGAGCTAAGCCTAAAGCTTGCAACCTGCAACTACCGATGCACCGGTTGTGATGCAATTAACGCCATTTA
CCCGGCAACAAAGGCGCTTGACGAGAGGGAGGGAACGGACTATCACAGGAGGTTTTTGGAACTGCTCAAAGAGATCCAGA
AGAAGGATTATGCCTGCACCGCCGCGCTGACGGATGTTAAGGGGGATAGGGGAAAGAGGCCCTCGGAGCAAAAGGACAAG
TACCTTCGTGTGGTTGAGAAGAGAGAAGATGGGATAGTGGTCAGAGGAGCTAAGATTTGCCAGAGCGGTGCCTTTGCGGC
AGACGTCAACTTCGTCTTACCAACTCAGACCTTCCGTCCAGGAGAGGAGGAATTCGCTGTCGTTTTTGCCGCCATGCCCG
AGGACGAGGGGATAAAGTATATCCTTCAGAACACTGGAATTCAGGCGAAGCAGCGCGAAGGGGGAAAGTTTGAGGCAGGA
AATAAGTACGGAGACAGAACAACCTGCATGGTTGTGTTTGACGACGTCTTCATACCGTGGGAAAGGGTTTTCGTTTTTGA
GGACTTGAAGGCGGCGAGGGACGTGGTCAGCTACTTCGCACCCTCTCACAGGTGTGTGGGGGCAGCGTGCAAGGCAGGGT
TTATCGACTCGATGGCCGGTGCAGCGAGTCTAATGCTAAAGACGAGCGGTCTTGAGAACGTTTCAGCTTTGCAGCAGAAA
GTGGCTGAGATGGTTGCAGTCAGCGAGGGTGCCTTTGCAATCTCCGTCGGTGCTGCGGTGAAGGGGGCAAATCACAACGG
CTGCTGGTTGCCCAACTTTCAGATGGCAAATTCCGGGAAGGTTATAGGTGTAGAGGGGTTCGCCAAAGCTCTGATGAACC
TGATAGACATTTCGGGAGGGATTATAGGCACCTCTCCTTCAGAATTTGACCTTGAAATGGTTGGGGACTTCCTCCGGGCA
AGCGAGAAATTTACCGCGGAGCAGAGACTTAAAATCGTGAAGTTCATCGAATTTTGGACTACTTCATCCCACCTGGTGGG
AGCATTCCACGGCGGAGGCTCACCAACGGCCGCCCTCATTACCCTGCGGTTTTTGATGGACTTGAGCAGCAGAGAGGAGG
CTGTCAAGAACTGCATTGACTTTTAA

Upstream 100 bases:

>100_bases
TCCCCACCACATAGAATCGACAATTTTGGAAGCCATAATGATGTACAAATAGAAATGCTTTTAAAAAAGAAATTCTATCA
GGATTGATTGAGGTGGAACT

Downstream 100 bases:

>100_bases
TTTTTTGTGTGAGTTTCCAAGCACAAAAGGATAATGCTCCGGCCGGGATTTGAACCCGGGTCGCCGGCTCTCTTCGGCGA
AACCTCGAAAGGCCGGAATG

Product: 4-hydroxyphenylacetate-3-hydroxylase (hpaA-2)

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLLKPEEYIERLGEYKRDIYVLGKKIEDFVNHPNIRPTVEAMAYTYSIAEKYAPHSELVGEKVNRLNYVNQTFEDLIARY
DYQRELSLKLATCNYRCTGCDAINAIYPATKALDEREGTDYHRRFLELLKEIQKKDYACTAALTDVKGDRGKRPSEQKDK
YLRVVEKREDGIVVRGAKICQSGAFAADVNFVLPTQTFRPGEEEFAVVFAAMPEDEGIKYILQNTGIQAKQREGGKFEAG
NKYGDRTTCMVVFDDVFIPWERVFVFEDLKAARDVVSYFAPSHRCVGAACKAGFIDSMAGAASLMLKTSGLENVSALQQK
VAEMVAVSEGAFAISVGAAVKGANHNGCWLPNFQMANSGKVIGVEGFAKALMNLIDISGGIIGTSPSEFDLEMVGDFLRA
SEKFTAEQRLKIVKFIEFWTTSSHLVGAFHGGGSPTAALITLRFLMDLSSREEAVKNCIDF

Sequences:

>Translated_461_residues
MLLKPEEYIERLGEYKRDIYVLGKKIEDFVNHPNIRPTVEAMAYTYSIAEKYAPHSELVGEKVNRLNYVNQTFEDLIARY
DYQRELSLKLATCNYRCTGCDAINAIYPATKALDEREGTDYHRRFLELLKEIQKKDYACTAALTDVKGDRGKRPSEQKDK
YLRVVEKREDGIVVRGAKICQSGAFAADVNFVLPTQTFRPGEEEFAVVFAAMPEDEGIKYILQNTGIQAKQREGGKFEAG
NKYGDRTTCMVVFDDVFIPWERVFVFEDLKAARDVVSYFAPSHRCVGAACKAGFIDSMAGAASLMLKTSGLENVSALQQK
VAEMVAVSEGAFAISVGAAVKGANHNGCWLPNFQMANSGKVIGVEGFAKALMNLIDISGGIIGTSPSEFDLEMVGDFLRA
SEKFTAEQRLKIVKFIEFWTTSSHLVGAFHGGGSPTAALITLRFLMDLSSREEAVKNCIDF
>Mature_461_residues
MLLKPEEYIERLGEYKRDIYVLGKKIEDFVNHPNIRPTVEAMAYTYSIAEKYAPHSELVGEKVNRLNYVNQTFEDLIARY
DYQRELSLKLATCNYRCTGCDAINAIYPATKALDEREGTDYHRRFLELLKEIQKKDYACTAALTDVKGDRGKRPSEQKDK
YLRVVEKREDGIVVRGAKICQSGAFAADVNFVLPTQTFRPGEEEFAVVFAAMPEDEGIKYILQNTGIQAKQREGGKFEAG
NKYGDRTTCMVVFDDVFIPWERVFVFEDLKAARDVVSYFAPSHRCVGAACKAGFIDSMAGAASLMLKTSGLENVSALQQK
VAEMVAVSEGAFAISVGAAVKGANHNGCWLPNFQMANSGKVIGVEGFAKALMNLIDISGGIIGTSPSEFDLEMVGDFLRA
SEKFTAEQRLKIVKFIEFWTTSSHLVGAFHGGGSPTAALITLRFLMDLSSREEAVKNCIDF

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 51218; Mature: 51218

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKPEEYIERLGEYKRDIYVLGKKIEDFVNHPNIRPTVEAMAYTYSIAEKYAPHSELVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
EKVNRLNYVNQTFEDLIARYDYQRELSLKLATCNYRCTGCDAINAIYPATKALDEREGTD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCHHHHHHHHHHHHHHCCCCH
YHRRFLELLKEIQKKDYACTAALTDVKGDRGKRPSEQKDKYLRVVEKREDGIVVRGAKIC
HHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHH
QSGAFAADVNFVLPTQTFRPGEEEFAVVFAAMPEDEGIKYILQNTGIQAKQREGGKFEAG
CCCCCEEECEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCC
NKYGDRTTCMVVFDDVFIPWERVFVFEDLKAARDVVSYFAPSHRCVGAACKAGFIDSMAG
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AASLMLKTSGLENVSALQQKVAEMVAVSEGAFAISVGAAVKGANHNGCWLPNFQMANSGK
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCCCEECCCCC
VIGVEGFAKALMNLIDISGGIIGTSPSEFDLEMVGDFLRASEKFTAEQRLKIVKFIEFWT
EEEHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
TSSHLVGAFHGGGSPTAALITLRFLMDLSSREEAVKNCIDF
CCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLLKPEEYIERLGEYKRDIYVLGKKIEDFVNHPNIRPTVEAMAYTYSIAEKYAPHSELVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
EKVNRLNYVNQTFEDLIARYDYQRELSLKLATCNYRCTGCDAINAIYPATKALDEREGTD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCHHHHHHHHHHHHHHCCCCH
YHRRFLELLKEIQKKDYACTAALTDVKGDRGKRPSEQKDKYLRVVEKREDGIVVRGAKIC
HHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHH
QSGAFAADVNFVLPTQTFRPGEEEFAVVFAAMPEDEGIKYILQNTGIQAKQREGGKFEAG
CCCCCEEECEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCC
NKYGDRTTCMVVFDDVFIPWERVFVFEDLKAARDVVSYFAPSHRCVGAACKAGFIDSMAG
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AASLMLKTSGLENVSALQQKVAEMVAVSEGAFAISVGAAVKGANHNGCWLPNFQMANSGK
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCCCEECCCCC
VIGVEGFAKALMNLIDISGGIIGTSPSEFDLEMVGDFLRASEKFTAEQRLKIVKFIEFWT
EEEHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
TSSHLVGAFHGGGSPTAALITLRFLMDLSSREEAVKNCIDF
CCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]