BacMap

Definition	Archaeoglobus fulgidus DSM 4304, complete genome.
Accession	NC_000917
Length	2,178,400

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The map label for this gene is fad-1

Identifier: 11498047

GI number: 11498047

Start: 392235

End: 393005

Strand: Reverse

Name: fad-1

Synonym: AF0435

Alternate gene names: 11498047

Gene position: 393005-392235 (Counterclockwise)

Preceding gene: 11498048

Following gene: 11498046

Centisome position: 18.04

GC content: 53.05

Gene sequence:

>771_bases
ATGGGAGAGAGAGTCAAGCTTGAGCTTGATGGTGAAATTGCTGTTGCAACCCTCAACAGACCAGAGAAGCTAAACGCTCT
TGACACCAAAACGAGGATGGAGCTGGCTGAGGTTATTGAGGGCATCGAGGAAGTGGCAAGAGTTTTAATCATTACCGGCA
GCGGCAAGGCCTTTGCTGCCGGCGCTGACATCAACGAGCTTCTTCAGAGGGATGCAATTAAGGCTTTTGAGGCAACAAAG
CTCGGAACGGATCTCTTTTCGAGAATTGAGGAGCTTGAGATTCCGGTGATTGCCGCAGTTAACGGATACACCCTCGGTGG
GGGTTGTGAGCTGGCGATGGCGTGCGACATAAGAATTGCCAGTGAAAAGGCAAAGTTCGGGCAGCCAGAGATAAACCTCG
CCATAATTCCCGGAGCGGGAGGGACGCAGAGGCTGCCGAGGCTCGTGGGGCTGGGGATGGCGAAAAAGCTTGTTCTTACG
GGGGAGATAATCGATGCTCAGACTGCTTTAAGAATAGGGCTGGTTGAGGAGGTTGTGGAGCACGAAAGGCTGATGGAGAG
GGCAAAGGAGGTTGCCGCTAAAATAATCGAGAAATCGCCTCTCGCTGTAAAGGTGGCGAAGAAAGCTTTGAACGCATCAA
TCAACATGCCCCTGAAGGAGGGGCTGAGGTATGAGGCAAGCCTCTTCGCCCTGCTCTTCTCCAGCGAGGATGCTAAGGAG
GGGATGAGGGCGTTTCTGGAGAAGAGGAAGCCTGAGTTTAGGGGAAGGTGA

Upstream 100 bases:

>100_bases
CTACAGGGATGCGAAGCTGATGGAGATTTACGAGGGAACGAAAGAGGTTCAGAAGCAGATAATTGCGAGAAGGTTGATAG
GAAAGCTTTGAGGTGGGAGA

Downstream 100 bases:

>100_bases
TGGCATGTTCGAGAAGATTGGAGTTGTCGGATTCGGCCTGATGGGCACCCAGATAACCCAATTTTTTGCCCAGCAGGGTT
TGGAGGTTGTTGCGATTGAC

Product: enoyl-CoA hydratase (fad-1)

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MGERVKLELDGEIAVATLNRPEKLNALDTKTRMELAEVIEGIEEVARVLIITGSGKAFAAGADINELLQRDAIKAFEATK
LGTDLFSRIEELEIPVIAAVNGYTLGGGCELAMACDIRIASEKAKFGQPEINLAIIPGAGGTQRLPRLVGLGMAKKLVLT
GEIIDAQTALRIGLVEEVVEHERLMERAKEVAAKIIEKSPLAVKVAKKALNASINMPLKEGLRYEASLFALLFSSEDAKE
GMRAFLEKRKPEFRGR

Sequences:

>Translated_256_residues
MGERVKLELDGEIAVATLNRPEKLNALDTKTRMELAEVIEGIEEVARVLIITGSGKAFAAGADINELLQRDAIKAFEATK
LGTDLFSRIEELEIPVIAAVNGYTLGGGCELAMACDIRIASEKAKFGQPEINLAIIPGAGGTQRLPRLVGLGMAKKLVLT
GEIIDAQTALRIGLVEEVVEHERLMERAKEVAAKIIEKSPLAVKVAKKALNASINMPLKEGLRYEASLFALLFSSEDAKE
GMRAFLEKRKPEFRGR
>Mature_255_residues
GERVKLELDGEIAVATLNRPEKLNALDTKTRMELAEVIEGIEEVARVLIITGSGKAFAAGADINELLQRDAIKAFEATKL
GTDLFSRIEELEIPVIAAVNGYTLGGGCELAMACDIRIASEKAKFGQPEINLAIIPGAGGTQRLPRLVGLGMAKKLVLTG
EIIDAQTALRIGLVEEVVEHERLMERAKEVAAKIIEKSPLAVKVAKKALNASINMPLKEGLRYEASLFALLFSSEDAKEG
MRAFLEKRKPEFRGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=45.7831325301205, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=34.8, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI20127408, Length=207, Percent_Identity=37.1980676328502, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI70995211, Length=254, Percent_Identity=33.0708661417323, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI68989263, Length=277, Percent_Identity=31.0469314079422, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=32.8125, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=29.957805907173, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=29.957805907173, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=30.8943089430894, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI157694520, Length=196, Percent_Identity=30.6122448979592, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI261878539, Length=179, Percent_Identity=31.8435754189944, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI37594469, Length=218, Percent_Identity=31.1926605504587, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594471, Length=218, Percent_Identity=31.651376146789, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI221136756, Length=219, Percent_Identity=24.6575342465753, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136753, Length=219, Percent_Identity=24.6575342465753, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=216, Percent_Identity=24.537037037037, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=21.875, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=21.875, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI62530384, Length=258, Percent_Identity=24.031007751938, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=262, Percent_Identity=21.7557251908397, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI49355787, Length=262, Percent_Identity=21.7557251908397, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4757966, Length=245, Percent_Identity=21.6326530612245, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI51538581, Length=245, Percent_Identity=21.6326530612245, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=242, Percent_Identity=47.1074380165289, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=39.4736842105263, Blast_Score=170, Evalue=8e-44,
Organism=Escherichia coli, GI1790281, Length=196, Percent_Identity=38.7755102040816, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=39.9038461538462, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=35.1778656126482, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=32.6848249027237, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=32.421875, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=44.4015444015444, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=44.5344129554656, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=40.5286343612335, Blast_Score=154, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17534483, Length=252, Percent_Identity=30.1587301587302, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17536985, Length=254, Percent_Identity=29.5275590551181, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17508953, Length=230, Percent_Identity=33.4782608695652, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=230, Percent_Identity=33.4782608695652, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=230, Percent_Identity=33.4782608695652, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=230, Percent_Identity=30.8695652173913, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=255, Percent_Identity=27.843137254902, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=29.8319327731092, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=237, Percent_Identity=29.1139240506329, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=32.8282828282828, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=32.8282828282828, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=32.2404371584699, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=45.3125, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=45.3125, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=38.4615384615385, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19920382, Length=253, Percent_Identity=32.8063241106719, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21357171, Length=267, Percent_Identity=28.4644194756554, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922422, Length=242, Percent_Identity=27.2727272727273, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571729, Length=302, Percent_Identity=26.8211920529801, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28571730, Length=302, Percent_Identity=26.8211920529801, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921000, Length=178, Percent_Identity=37.0786516853933, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583077, Length=178, Percent_Identity=37.0786516853933, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583079, Length=178, Percent_Identity=37.0786516853933, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=30.4, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583165, Length=240, Percent_Identity=27.5, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654903, Length=183, Percent_Identity=27.8688524590164, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921018, Length=246, Percent_Identity=28.4552845528455, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550169, Length=208, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 27765; Mature: 27634

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGERVKLELDGEIAVATLNRPEKLNALDTKTRMELAEVIEGIEEVARVLIITGSGKAFAA
CCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEC
GADINELLQRDAIKAFEATKLGTDLFSRIEELEIPVIAAVNGYTLGGGCELAMACDIRIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEEEEEEEEEEE
SEKAKFGQPEINLAIIPGAGGTQRLPRLVGLGMAKKLVLTGEIIDAQTALRIGLVEEVVE
CCHHHCCCCCEEEEEEECCCCHHHHHHHHHCCHHHHHEEECCHHHHHHHHHHHHHHHHHH
HERLMERAKEVAAKIIEKSPLAVKVAKKALNASINMPLKEGLRYEASLFALLFSSEDAKE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCHHH
GMRAFLEKRKPEFRGR
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GERVKLELDGEIAVATLNRPEKLNALDTKTRMELAEVIEGIEEVARVLIITGSGKAFAA
CCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEC
GADINELLQRDAIKAFEATKLGTDLFSRIEELEIPVIAAVNGYTLGGGCELAMACDIRIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEEEEEEEEEEE
SEKAKFGQPEINLAIIPGAGGTQRLPRLVGLGMAKKLVLTGEIIDAQTALRIGLVEEVVE
CCHHHCCCCCEEEEEEECCCCHHHHHHHHHCCHHHHHEEECCHHHHHHHHHHHHHHHHHH
HERLMERAKEVAAKIIEKSPLAVKVAKKALNASINMPLKEGLRYEASLFALLFSSEDAKE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCHHH
GMRAFLEKRKPEFRGR
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]