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The map label for this gene is graD-2

Identifier: 11497938

GI number: 11497938

Start: 295251

End: 296309

Strand: Reverse

Name: graD-2

Synonym: AF0325

Alternate gene names: 11497938

Gene position: 296309-295251 (Counterclockwise)

Preceding gene: 11497942

Following gene: 11497937

Centisome position: 13.6

GC content: 47.03

Gene sequence:

>1059_bases
GTGAAGGGCGTACTGCTTCACGGTGGTGCCGGCACCCGATTAAGACCACTAACCTTTACGGGGCCAAAGCAGCTGATTCC
CGTAGCCAACAAGCCGGTTTCCCAGTATTGCCTTGAGGACATGATTGGTGCAGGAATCAAAGAGGTAGCCATAATTTTGG
GGGAGACCTATCCAGAAATGGTGGAAGAGCACTACGGAGATGGTTCGAGGTTCGGATGCAAGATTACATACATCCATCAG
GGTAAGCCACTCGGTATTGCTCATGCAGTCTATCTTGCCAAAGACTTCGTCGGTGATGAAAAGTTTGTAGTTTATCTCGG
CGACAACCTGATTCAGGATGGTATAAAGGAATACGTTAAGAGATTTGACGAGGAAGATTTTGACGCCTTCATACTTCTCA
AGGAGGTTGAGGATCCAAGAGCCTTTGGTGTTGCCAAGTTTGAGGGCGAGAGACTGGTCGGACTCATTGAGAAGCCAAAA
GAACCTCCAAGCAACTACGCGGTAATTGGTGTATATATGTTCAAACCAGTTGTTTTTGACATAATCAAAGACCTAAAACC
GAGCTGGAGAGGAGAACTTGAAATCACCGACACGCTACAAAAAATGATTGAAAACGGTTACAATGTGGGATATGCTAAAC
TGAAGGGTTGGTGGTTCGACACAGGCAAGGCAGAGGACATACTGAAGGTGAACGCAACGATTCTCGATGAGAGGGCAAAA
AGGAGTGTAAAGGGAGAGGTTTTGGCGTCGCAAATTGAGGGAAGAGTTGAGGTTGGAGAAGGGGCAAAAATAACTAACAG
CATCGTAAGGGGGCCAGCGGTCATCGGGGAAGACTGCATAATCGAGAACTCGTTCATCGGGCCCTACACGAGCGTTGGTG
AAAGAACGGTCATTAAAAACAGCTCGCTTGAATACTGCATAGTTCTCAGCGAGTCAATTATCGAGGGTGTGGAGAGAATT
GAGGAGAGTCTCATTGGGAGGAAAGTCAGAATCAGAAAGAACGACAGAAAGAGGTTCCTAAGATTCACCATTGGGGATTA
CTCCGAGATCCTGCTTTGA

Upstream 100 bases:

>100_bases
TTATCTGGAGGAGGATGACATAGAAAGGTTCGAGGACGATTTTGGGAGAGGTTAGAGTTAAAACCGACAATATTTTATAA
ATCAATTTCCCTCTTTTATC

Downstream 100 bases:

>100_bases
GGTGGAAGATGAGAATTCTTGTTACGGGAGGAATGGGGTTCATCGGCTCCAACTTCGTTAGGTACGCTCTTGCTCACCAC
CAGGTAGAGATTCTGAACCT

Product: glucose-1-phosphate thymidylyltransferase (graD-2)

Products: NA

Alternate protein names: Sugar-nucleotidylation enzyme; dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKGVLLHGGAGTRLRPLTFTGPKQLIPVANKPVSQYCLEDMIGAGIKEVAIILGETYPEMVEEHYGDGSRFGCKITYIHQ
GKPLGIAHAVYLAKDFVGDEKFVVYLGDNLIQDGIKEYVKRFDEEDFDAFILLKEVEDPRAFGVAKFEGERLVGLIEKPK
EPPSNYAVIGVYMFKPVVFDIIKDLKPSWRGELEITDTLQKMIENGYNVGYAKLKGWWFDTGKAEDILKVNATILDERAK
RSVKGEVLASQIEGRVEVGEGAKITNSIVRGPAVIGEDCIIENSFIGPYTSVGERTVIKNSSLEYCIVLSESIIEGVERI
EESLIGRKVRIRKNDRKRFLRFTIGDYSEILL

Sequences:

>Translated_352_residues
MKGVLLHGGAGTRLRPLTFTGPKQLIPVANKPVSQYCLEDMIGAGIKEVAIILGETYPEMVEEHYGDGSRFGCKITYIHQ
GKPLGIAHAVYLAKDFVGDEKFVVYLGDNLIQDGIKEYVKRFDEEDFDAFILLKEVEDPRAFGVAKFEGERLVGLIEKPK
EPPSNYAVIGVYMFKPVVFDIIKDLKPSWRGELEITDTLQKMIENGYNVGYAKLKGWWFDTGKAEDILKVNATILDERAK
RSVKGEVLASQIEGRVEVGEGAKITNSIVRGPAVIGEDCIIENSFIGPYTSVGERTVIKNSSLEYCIVLSESIIEGVERI
EESLIGRKVRIRKNDRKRFLRFTIGDYSEILL
>Mature_352_residues
MKGVLLHGGAGTRLRPLTFTGPKQLIPVANKPVSQYCLEDMIGAGIKEVAIILGETYPEMVEEHYGDGSRFGCKITYIHQ
GKPLGIAHAVYLAKDFVGDEKFVVYLGDNLIQDGIKEYVKRFDEEDFDAFILLKEVEDPRAFGVAKFEGERLVGLIEKPK
EPPSNYAVIGVYMFKPVVFDIIKDLKPSWRGELEITDTLQKMIENGYNVGYAKLKGWWFDTGKAEDILKVNATILDERAK
RSVKGEVLASQIEGRVEVGEGAKITNSIVRGPAVIGEDCIIENSFIGPYTSVGERTVIKNSSLEYCIVLSESIIEGVERI
EESLIGRKVRIRKNDRKRFLRFTIGDYSEILL

Specific function: Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=336, Percent_Identity=27.9761904761905, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI11761619, Length=336, Percent_Identity=27.9761904761905, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1790224, Length=240, Percent_Identity=37.0833333333333, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788351, Length=246, Percent_Identity=34.1463414634146, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787488, Length=262, Percent_Identity=29.7709923664122, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788355, Length=264, Percent_Identity=24.6212121212121, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI133931050, Length=336, Percent_Identity=29.7619047619048, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320148, Length=331, Percent_Identity=28.7009063444109, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21355443, Length=337, Percent_Identity=31.7507418397626, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24644084, Length=337, Percent_Identity=31.7507418397626, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005908
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 39403; Mature: 39403

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVLLHGGAGTRLRPLTFTGPKQLIPVANKPVSQYCLEDMIGAGIKEVAIILGETYPEM
CCCEEEECCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHH
VEEHYGDGSRFGCKITYIHQGKPLGIAHAVYLAKDFVGDEKFVVYLGDNLIQDGIKEYVK
HHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
RFDEEDFDAFILLKEVEDPRAFGVAKFEGERLVGLIEKPKEPPSNYAVIGVYMFKPVVFD
HCCCCCCCEEEEEEECCCCCCCCEEEECCCHHEEHHHCCCCCCCCEEEEEHHHHHHHHHH
IIKDLKPSWRGELEITDTLQKMIENGYNVGYAKLKGWWFDTGKAEDILKVNATILDERAK
HHHHCCCCCCCCEEHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEHHHHHHHHHH
RSVKGEVLASQIEGRVEVGEGAKITNSIVRGPAVIGEDCIIENSFIGPYTSVGERTVIKN
HHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCEECCCCEEECCCCCCCCCCCCEEEEEC
SSLEYCIVLSESIIEGVERIEESLIGRKVRIRKNDRKRFLRFTIGDYSEILL
CCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCHHHHCC
>Mature Secondary Structure
MKGVLLHGGAGTRLRPLTFTGPKQLIPVANKPVSQYCLEDMIGAGIKEVAIILGETYPEM
CCCEEEECCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHH
VEEHYGDGSRFGCKITYIHQGKPLGIAHAVYLAKDFVGDEKFVVYLGDNLIQDGIKEYVK
HHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
RFDEEDFDAFILLKEVEDPRAFGVAKFEGERLVGLIEKPKEPPSNYAVIGVYMFKPVVFD
HCCCCCCCEEEEEEECCCCCCCCEEEECCCHHEEHHHCCCCCCCCEEEEEHHHHHHHHHH
IIKDLKPSWRGELEITDTLQKMIENGYNVGYAKLKGWWFDTGKAEDILKVNATILDERAK
HHHHCCCCCCCCEEHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEHHHHHHHHHH
RSVKGEVLASQIEGRVEVGEGAKITNSIVRGPAVIGEDCIIENSFIGPYTSVGERTVIKN
HHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCEECCCCEEECCCCCCCCCCCCEEEEEC
SSLEYCIVLSESIIEGVERIEESLIGRKVRIRKNDRKRFLRFTIGDYSEILL
CCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3118332 [H]