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The map label for this gene is rutB [H]

Identifier: 114800521

GI number: 114800521

Start: 1282015

End: 1282665

Strand: Reverse

Name: rutB [H]

Synonym: HNE_1257

Alternate gene names: 114800521

Gene position: 1282665-1282015 (Counterclockwise)

Preceding gene: 114797899

Following gene: 114799386

Centisome position: 34.62

GC content: 64.98

Gene sequence:

>651_bases
ATGCCTGTGAGCCTGAAACCCGCTTTTCCTGACACCCTTTCTGGCTGGATCGCGCCAGACCGCACGGCGGTCATCGTTGT
CGATATTCAGGTCGATTTTGCCTCACCCGATGGCGTGCTGGGCAAGGCCGGCGTGGACATGGGCATCGCGGCGCCGGCGG
TCTCTGCTGCTGAACGTCTGGTCGATGCCGCCCGCGCCGCCGGAACGCCCGTCATCTTCGTCGGCCTCGCCACCACCGCC
GCAACGGACTCGCCCGTCTGGGGCGAATGGCGCCGCCGCAAGAACGGTGTCGACAGTGGTGGCGCGCTCTGCCGCGCAGG
CGAAGTGGGCTCGGCTTTCTATGGCCCTACGCCCCAGGGCGATGAAACCGTCATCTGGAAACTGCGCTATAGCGGCTTCT
TCGGCACCAGCCTGGACGCGGCCCTGCGCGCGCGCGGCATCGACACGCTGATCGTCTGCGGCCTCACCACGGAATGCTGT
GTCGATTGCACCGTGCGCGATGCCTTCCACCTCGATTATTTCGTGTACGTGCCAACCGACGCCTGCGCGGCCTACGATCC
CGGCATCCACGAAAGCGCGCTGCAATCCCTGGAGCTCAACTGCGCCACGCTCATCTCTACCGGTGAGATCCTCAGTGCCT
GGACTGAATAA

Upstream 100 bases:

>100_bases
GCCGGGGACCGGTTCTGCTGCCTACCCAACGCCCTCCCGCTGGGCTTGCAGGACCGGTCCTTGGCAGAGGAGACACTATG
GGGAGTGATAACAATGAGGA

Downstream 100 bases:

>100_bases
AAACAAGAAAGTGTCCCCATGAGTAACGTGTTCACGAATTTGCCCGAGGCGAACTATATCGTCACGGAAGAAGATGCCGC
CGCCCTCGCCGCCGCTATCG

Product: isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MPVSLKPAFPDTLSGWIAPDRTAVIVVDIQVDFASPDGVLGKAGVDMGIAAPAVSAAERLVDAARAAGTPVIFVGLATTA
ATDSPVWGEWRRRKNGVDSGGALCRAGEVGSAFYGPTPQGDETVIWKLRYSGFFGTSLDAALRARGIDTLIVCGLTTECC
VDCTVRDAFHLDYFVYVPTDACAAYDPGIHESALQSLELNCATLISTGEILSAWTE

Sequences:

>Translated_216_residues
MPVSLKPAFPDTLSGWIAPDRTAVIVVDIQVDFASPDGVLGKAGVDMGIAAPAVSAAERLVDAARAAGTPVIFVGLATTA
ATDSPVWGEWRRRKNGVDSGGALCRAGEVGSAFYGPTPQGDETVIWKLRYSGFFGTSLDAALRARGIDTLIVCGLTTECC
VDCTVRDAFHLDYFVYVPTDACAAYDPGIHESALQSLELNCATLISTGEILSAWTE
>Mature_215_residues
PVSLKPAFPDTLSGWIAPDRTAVIVVDIQVDFASPDGVLGKAGVDMGIAAPAVSAAERLVDAARAAGTPVIFVGLATTAA
TDSPVWGEWRRRKNGVDSGGALCRAGEVGSAFYGPTPQGDETVIWKLRYSGFFGTSLDAALRARGIDTLIVCGLTTECCV
DCTVRDAFHLDYFVYVPTDACAAYDPGIHESALQSLELNCATLISTGEILSAWTE

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=191, Percent_Identity=34.5549738219895, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI87081992, Length=196, Percent_Identity=30.1020408163265, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 22661; Mature: 22530

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSLKPAFPDTLSGWIAPDRTAVIVVDIQVDFASPDGVLGKAGVDMGIAAPAVSAAERL
CCCCCCCCCCCHHCCCCCCCCCEEEEEEEEEEECCCCCCEECCCCCCCCCCHHHHHHHHH
VDAARAAGTPVIFVGLATTAATDSPVWGEWRRRKNGVDSGGALCRAGEVGSAFYGPTPQG
HHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCC
DETVIWKLRYSGFFGTSLDAALRARGIDTLIVCGLTTECCVDCTVRDAFHLDYFVYVPTD
CCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHEECEECCEEEEEEEEEECCC
ACAAYDPGIHESALQSLELNCATLISTGEILSAWTE
HHHCCCCCCCHHHHHHHCCCHHEEEECCHHHHHCCC
>Mature Secondary Structure 
PVSLKPAFPDTLSGWIAPDRTAVIVVDIQVDFASPDGVLGKAGVDMGIAAPAVSAAERL
CCCCCCCCCCHHCCCCCCCCCEEEEEEEEEEECCCCCCEECCCCCCCCCCHHHHHHHHH
VDAARAAGTPVIFVGLATTAATDSPVWGEWRRRKNGVDSGGALCRAGEVGSAFYGPTPQG
HHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCC
DETVIWKLRYSGFFGTSLDAALRARGIDTLIVCGLTTECCVDCTVRDAFHLDYFVYVPTD
CCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHEECEECCEEEEEEEEEECCC
ACAAYDPGIHESALQSLELNCATLISTGEILSAWTE
HHHCCCCCCCHHHHHHHCCCHHEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA