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The map label for this gene is rfaE

Identifier: 114800169

GI number: 114800169

Start: 1844994

End: 1846454

Strand: Direct

Name: rfaE

Synonym: HNE_1799

Alternate gene names: 114800169

Gene position: 1844994-1846454 (Clockwise)

Preceding gene: 114800487

Following gene: 114799621

Centisome position: 49.8

GC content: 60.37

Gene sequence:

>1461_bases
ATGTCATCCCGACTTTCAGGGCTTCTAGACCGTGCGGCCGGAAAGCGCGTTCTGTGCATCGGAGACGTGATGCTGGACCG
CTTCATTTACGGCGTCGTGGATCGCATATCGCCCGAAGCGCCTGTTCCGGTTCTTCGCCATAGCCGGGAAGCGTCAATGC
CGGGCGGCGCTGCCAACGTTGCCCGCAACCTCGCCTCGCTTGGTCTGGAACCCGTATTAATCGGTGCCTGCGGCGATGAT
GACGCCGGAAGGGAACTTCTGTCCATATTTGACCAGGATTTGTCTCTTTCTGTCAGGCTCGTGACGGCCAAGGGCCGCCC
GACAACCCTGAAATGCCGCTTCGTGGCCGGAGGGCATCAGCTTCTGCGCGTCGATACCGAAAACGTCGCTCCTGTCAGTG
AAGCGACAGAAGAGGAGCTTATCGGTATTCTGTCTCGAGAGGCCCCCGGATCGGCCGCTATCCTCATCTCCGATTATGCA
AAGGGCCTTCTGACAGATCGGCTGCTGAAGGCGGTCACGAAGCTGGCCGCCGACCTGAATATCCCGCTCATTGCAGACCC
CAAAGGACGCGATTTTGCCCGCTATGGCGCTGTCGACATTCTCAAACCGAATGCATTCGAACTGTCGGCGGCGGTGCATA
GGAGCATCAGCACCGATGAAGAGGCCGCTCTGGCCTTGCGCGAAGCCTTGGACACCCTGCCAGCCAAAGCGATCATTGTA
ACACGAGCCGCACGTGGAATTTCCTATATCGGTCAAGACGGTAACGTGCATCATGAAGCGGGCAGGGCGCGGGAAGTCTT
CGACGTGTCCGGAGCGGGAGACACCAGCCTCGCAGCCCTCGCTACGGCGATCGCGGGCGGTGGCACCCTGTCGGATGCTG
TACATCTGGCAATTGCCGCATCCGGCATCGCAGTCGGCAAGGCAGGCACAGCAACGGTGTCGGCTGAAGAGATCAAGGCT
GCGCTGTCAGTCGCAGGCCCGGTTGGCCGTGCGGGCCTGCTGCCGATGGATGCCATGATTGGTCAGGTCGAGCGCTGGCG
CGCCGCCGGCCTGAAGATTGGCTTCACGAACGGGTGTTTTGACATCCTTCATCCCGGTCACATCCGGGTAATAGAGCAGG
CCCGCGCGCACTGCGACAGGCTGGTAGTGGGCTTGAACTCCGACAACTCTGTCAAACGCCTCAAAGGCCCGCTGCGTCCA
ATCAACAACGAGCAGGCGCGGGCTGATGTTCTGTCGGCACTGAGCGCAGTCGACGGCGTGATTATCTTCGATACGGACAC
ACCGCTGGACGCCATTGCCGCCCTCAATCCTGATGTGCTGGTGAAGGGCGGAGATTACACGCGTGAATCTATAGTGGGCG
CAGACATCGTTGAAGCCAGGGGCGGCGAGATCGTGATTGTGCCGCTGGTCGCAGGCCATTCTACCACCGCAATCATCGCG
CGAAGCGAAACCGGGAAATAG

Upstream 100 bases:

>100_bases
AATTGGATCACCCTTCAGCGCGCATCGCTTTCACTCGCGAAGGTCAACCGTTATAGCCAGTCGGCAAGACCTTTCATTTC
CCCCGGAGGCTCACATCAAC

Downstream 100 bases:

>100_bases
GCCGAAAAATAGGAAAAAAGGGACTGAACACCCGCAGGGATCAGTTGGTCGCCTCTTTCTTCAGTTCGTAAGTGCCACTG
CCATTCATCGTGAACCAGTC

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSSRLSGLLDRAAGKRVLCIGDVMLDRFIYGVVDRISPEAPVPVLRHSREASMPGGAANVARNLASLGLEPVLIGACGDD
DAGRELLSIFDQDLSLSVRLVTAKGRPTTLKCRFVAGGHQLLRVDTENVAPVSEATEEELIGILSREAPGSAAILISDYA
KGLLTDRLLKAVTKLAADLNIPLIADPKGRDFARYGAVDILKPNAFELSAAVHRSISTDEEAALALREALDTLPAKAIIV
TRAARGISYIGQDGNVHHEAGRAREVFDVSGAGDTSLAALATAIAGGGTLSDAVHLAIAASGIAVGKAGTATVSAEEIKA
ALSVAGPVGRAGLLPMDAMIGQVERWRAAGLKIGFTNGCFDILHPGHIRVIEQARAHCDRLVVGLNSDNSVKRLKGPLRP
INNEQARADVLSALSAVDGVIIFDTDTPLDAIAALNPDVLVKGGDYTRESIVGADIVEARGGEIVIVPLVAGHSTTAIIA
RSETGK

Sequences:

>Translated_486_residues
MSSRLSGLLDRAAGKRVLCIGDVMLDRFIYGVVDRISPEAPVPVLRHSREASMPGGAANVARNLASLGLEPVLIGACGDD
DAGRELLSIFDQDLSLSVRLVTAKGRPTTLKCRFVAGGHQLLRVDTENVAPVSEATEEELIGILSREAPGSAAILISDYA
KGLLTDRLLKAVTKLAADLNIPLIADPKGRDFARYGAVDILKPNAFELSAAVHRSISTDEEAALALREALDTLPAKAIIV
TRAARGISYIGQDGNVHHEAGRAREVFDVSGAGDTSLAALATAIAGGGTLSDAVHLAIAASGIAVGKAGTATVSAEEIKA
ALSVAGPVGRAGLLPMDAMIGQVERWRAAGLKIGFTNGCFDILHPGHIRVIEQARAHCDRLVVGLNSDNSVKRLKGPLRP
INNEQARADVLSALSAVDGVIIFDTDTPLDAIAALNPDVLVKGGDYTRESIVGADIVEARGGEIVIVPLVAGHSTTAIIA
RSETGK
>Mature_485_residues
SSRLSGLLDRAAGKRVLCIGDVMLDRFIYGVVDRISPEAPVPVLRHSREASMPGGAANVARNLASLGLEPVLIGACGDDD
AGRELLSIFDQDLSLSVRLVTAKGRPTTLKCRFVAGGHQLLRVDTENVAPVSEATEEELIGILSREAPGSAAILISDYAK
GLLTDRLLKAVTKLAADLNIPLIADPKGRDFARYGAVDILKPNAFELSAAVHRSISTDEEAALALREALDTLPAKAIIVT
RAARGISYIGQDGNVHHEAGRAREVFDVSGAGDTSLAALATAIAGGGTLSDAVHLAIAASGIAVGKAGTATVSAEEIKAA
LSVAGPVGRAGLLPMDAMIGQVERWRAAGLKIGFTNGCFDILHPGHIRVIEQARAHCDRLVVGLNSDNSVKRLKGPLRPI
NNEQARADVLSALSAVDGVIIFDTDTPLDAIAALNPDVLVKGGDYTRESIVGADIVEARGGEIVIVPLVAGHSTTAIIAR
SETGK

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=480, Percent_Identity=40.8333333333333, Blast_Score=314, Evalue=6e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_HYPNA (Q0C190)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760503.1
- ProteinModelPortal:   Q0C190
- SMR:   Q0C190
- STRING:   Q0C190
- GeneID:   4290013
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1799
- NMPDR:   fig|228405.5.peg.1732
- TIGR:   HNE_1799
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- PhylomeDB:   Q0C190
- ProtClustDB:   CLSK2317040
- BioCyc:   HNEP81032:HNE_1799-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 50542; Mature: 50411

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRLSGLLDRAAGKRVLCIGDVMLDRFIYGVVDRISPEAPVPVLRHSREASMPGGAANV
CCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH
ARNLASLGLEPVLIGACGDDDAGRELLSIFDQDLSLSVRLVTAKGRPTTLKCRFVAGGHQ
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEECCCE
LLRVDTENVAPVSEATEEELIGILSREAPGSAAILISDYAKGLLTDRLLKAVTKLAADLN
EEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCC
IPLIADPKGRDFARYGAVDILKPNAFELSAAVHRSISTDEEAALALREALDTLPAKAIIV
CCEEECCCCCCHHHHCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
TRAARGISYIGQDGNVHHEAGRAREVFDVSGAGDTSLAALATAIAGGGTLSDAVHLAIAA
EHHHCCCCEECCCCCEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHEEEEEE
SGIAVGKAGTATVSAEEIKAALSVAGPVGRAGLLPMDAMIGQVERWRAAGLKIGFTNGCF
CCEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCH
DILHPGHIRVIEQARAHCDRLVVGLNSDNSVKRLKGPLRPINNEQARADVLSALSAVDGV
HHCCCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCE
IIFDTDTPLDAIAALNPDVLVKGGDYTRESIVGADIVEARGGEIVIVPLVAGHSTTAIIA
EEEECCCCHHHHHHCCCCEEEECCCCCHHHHCCCHHHCCCCCCEEEEEEECCCCEEEEEE
RSETGK
ECCCCC
>Mature Secondary Structure 
SSRLSGLLDRAAGKRVLCIGDVMLDRFIYGVVDRISPEAPVPVLRHSREASMPGGAANV
CHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH
ARNLASLGLEPVLIGACGDDDAGRELLSIFDQDLSLSVRLVTAKGRPTTLKCRFVAGGHQ
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEECCCE
LLRVDTENVAPVSEATEEELIGILSREAPGSAAILISDYAKGLLTDRLLKAVTKLAADLN
EEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCC
IPLIADPKGRDFARYGAVDILKPNAFELSAAVHRSISTDEEAALALREALDTLPAKAIIV
CCEEECCCCCCHHHHCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
TRAARGISYIGQDGNVHHEAGRAREVFDVSGAGDTSLAALATAIAGGGTLSDAVHLAIAA
EHHHCCCCEECCCCCEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHEEEEEE
SGIAVGKAGTATVSAEEIKAALSVAGPVGRAGLLPMDAMIGQVERWRAAGLKIGFTNGCF
CCEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCH
DILHPGHIRVIEQARAHCDRLVVGLNSDNSVKRLKGPLRPINNEQARADVLSALSAVDGV
HHCCCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCE
IIFDTDTPLDAIAALNPDVLVKGGDYTRESIVGADIVEARGGEIVIVPLVAGHSTTAIIA
EEEECCCCHHHHHHCCCCEEEECCCCCHHHHCCCHHHCCCCCCEEEEEEECCCCEEEEEE
RSETGK
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA