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The map label for this gene is pyrG

Identifier: 114800487

GI number: 114800487

Start: 1838378

End: 1840012

Strand: Direct

Name: pyrG

Synonym: HNE_1794

Alternate gene names: 114800487

Gene position: 1838378-1840012 (Clockwise)

Preceding gene: 114799731

Following gene: 114800169

Centisome position: 49.62

GC content: 59.33

Gene sequence:

>1635_bases
ATGATCCGCTATATTTTCATCACTGGCGGCGTGGTGTCTTCGCTCGGAAAAGGCATAGCCTCCGCCTCGCTCGGCGCGCT
GCTGCAATCGCGCGGGTACCGCGTGCGCCTGCGCAAGCTCGATCCCTATCTCAACGTCGATCCGGGCACGATGAGCCCCC
GCCAGCATGGTGAGGTCTATGTGACCGACGATGGCGCAGAGACCGACCTCGATCTCGGCCATTATGAACGCTTTACTGGC
GTATCGGCCCGGCAGTCCGACAACATCACGACCGGCCGCATCTATCAGCGTATCATCGAGAAAGAACGTCGCGGCGATTA
TCTCGGCGCCACGATCCAGGTCATCCCTCACGTCACCAACGACATCAAGGAGTTCGTCCTTTCCGATCCCGGCGAGGGCG
TTGATTTTGTTCTCTGCGAAATCGGGGGAACGGTCGGGGACATCGAAGGCCTGCCATTCTTCGAGGCGATCCGTCAGCTC
GGCCAGGAACTGGGGCCTCAGCGGGCATGCTTTATACACCTGACACTGCTGCCCTACATCCCGGCGGCGGGCGAAATGAA
AACCAAACCGACGCAGCACTCGGTCAAGGAACTGCGGTCTATTGGCATCCAGCCACAGATTCTTCTTTGCCGCTGTGACC
GGCCGATCCCGGTCAATGAAAAGGGCAAGATCGCCAGCTTCTGCAATGTCCGCCTGGCAAGCGTGATCGAGGCCCGCGAC
GTCGGACATATCTATGACGTGCCGATGGCTTATCACGCGGAAGGACTGGACTCTGAGGTTCTGTCGCATTTCGGAATAAC
CGATGCCCCCCCGCCGGACCTGTCTTCCTGGCAGCGGATCGCCCAGACAATCAAAAACCCCGATGGTCAGGTGACAATTG
GCCTGGTCGGAAAATACACCGACGTACCAGATGCCTACAAATCGGTATCCGAAGCGCTCGGCCATGGCGGCCTCGCCAAC
AAGGTGAAGGTCGACATCCGATTCGTGGATTCCGAAAAATTCGACGATCCCGACGCCGCGCTTGAAGATTTGGATGGCGT
ACACGGAATTCTGCTGCCCGGCGGTTTCGGCGAACGTGGCGCACACGGCAAGATGCGGGTCGCCAGATATGCGCGTGAGC
GTAACATGCCGTGTTTCGGTATCTGCTACGGCATGCAGCTCTCGGTTGTTGAAGCTGCGCGTAATCTGGCCGGCATCAAG
AATGCTTCGACCAGCGAATTTGGCCCAACCAAAGAGCCCGTGGTTGGTCTCATGGAAGAGTGGACCAAGGGCAACGAGAA
GGTCACGCGCGACGCCAGCACCGACCTTGGCGGCACAATGCGTCTCGGCGCGTACCCTGCACGCCTCAAGGAAGGCAGCA
TGGTCGCACAGGTTTATGGCAGTCTCGAAATCTCCGAGCGCCACCGCCACCGCTACGAAGTCAATGCCAGTTACATCGAA
CGCCTCGAAAAGGCGGGTATGATCTTCTCAGGCATGTCGCCAGATGGCCGCCTCCCGGAAATCGTCGAGCTTGAAGGGCA
CCCCTGGTTCATCGGCGTGCAGTTCCACCCGGAACTCAAGTCCCGGCCGTTCGAACCCCACCCGCTTTTCGCGAGCTTCA
TTGCAGCGGCGGTCAAACAATCCCGGCTCGTCTGA

Upstream 100 bases:

>100_bases
TACGGAATCCGTGCCCGAAACGGCTAATCCCCAGTAATTTTCTGCTAATCGCTGGTGGAGGTCGCGCGCAAGGCGCGAAT
CATTTCATGAGTATAACCCC

Downstream 100 bases:

>100_bases
ACGACACGCCGGGCAGGGGCCCTCAGGCCGGCCGCGTTACCTGGAAATGGCCGACATTTATCCGGCCGTCGCTGAAGCCA
GCTTCGCAATAGCTGAGATA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MIRYIFITGGVVSSLGKGIASASLGALLQSRGYRVRLRKLDPYLNVDPGTMSPRQHGEVYVTDDGAETDLDLGHYERFTG
VSARQSDNITTGRIYQRIIEKERRGDYLGATIQVIPHVTNDIKEFVLSDPGEGVDFVLCEIGGTVGDIEGLPFFEAIRQL
GQELGPQRACFIHLTLLPYIPAAGEMKTKPTQHSVKELRSIGIQPQILLCRCDRPIPVNEKGKIASFCNVRLASVIEARD
VGHIYDVPMAYHAEGLDSEVLSHFGITDAPPPDLSSWQRIAQTIKNPDGQVTIGLVGKYTDVPDAYKSVSEALGHGGLAN
KVKVDIRFVDSEKFDDPDAALEDLDGVHGILLPGGFGERGAHGKMRVARYARERNMPCFGICYGMQLSVVEAARNLAGIK
NASTSEFGPTKEPVVGLMEEWTKGNEKVTRDASTDLGGTMRLGAYPARLKEGSMVAQVYGSLEISERHRHRYEVNASYIE
RLEKAGMIFSGMSPDGRLPEIVELEGHPWFIGVQFHPELKSRPFEPHPLFASFIAAAVKQSRLV

Sequences:

>Translated_544_residues
MIRYIFITGGVVSSLGKGIASASLGALLQSRGYRVRLRKLDPYLNVDPGTMSPRQHGEVYVTDDGAETDLDLGHYERFTG
VSARQSDNITTGRIYQRIIEKERRGDYLGATIQVIPHVTNDIKEFVLSDPGEGVDFVLCEIGGTVGDIEGLPFFEAIRQL
GQELGPQRACFIHLTLLPYIPAAGEMKTKPTQHSVKELRSIGIQPQILLCRCDRPIPVNEKGKIASFCNVRLASVIEARD
VGHIYDVPMAYHAEGLDSEVLSHFGITDAPPPDLSSWQRIAQTIKNPDGQVTIGLVGKYTDVPDAYKSVSEALGHGGLAN
KVKVDIRFVDSEKFDDPDAALEDLDGVHGILLPGGFGERGAHGKMRVARYARERNMPCFGICYGMQLSVVEAARNLAGIK
NASTSEFGPTKEPVVGLMEEWTKGNEKVTRDASTDLGGTMRLGAYPARLKEGSMVAQVYGSLEISERHRHRYEVNASYIE
RLEKAGMIFSGMSPDGRLPEIVELEGHPWFIGVQFHPELKSRPFEPHPLFASFIAAAVKQSRLV
>Mature_544_residues
MIRYIFITGGVVSSLGKGIASASLGALLQSRGYRVRLRKLDPYLNVDPGTMSPRQHGEVYVTDDGAETDLDLGHYERFTG
VSARQSDNITTGRIYQRIIEKERRGDYLGATIQVIPHVTNDIKEFVLSDPGEGVDFVLCEIGGTVGDIEGLPFFEAIRQL
GQELGPQRACFIHLTLLPYIPAAGEMKTKPTQHSVKELRSIGIQPQILLCRCDRPIPVNEKGKIASFCNVRLASVIEARD
VGHIYDVPMAYHAEGLDSEVLSHFGITDAPPPDLSSWQRIAQTIKNPDGQVTIGLVGKYTDVPDAYKSVSEALGHGGLAN
KVKVDIRFVDSEKFDDPDAALEDLDGVHGILLPGGFGERGAHGKMRVARYARERNMPCFGICYGMQLSVVEAARNLAGIK
NASTSEFGPTKEPVVGLMEEWTKGNEKVTRDASTDLGGTMRLGAYPARLKEGSMVAQVYGSLEISERHRHRYEVNASYIE
RLEKAGMIFSGMSPDGRLPEIVELEGHPWFIGVQFHPELKSRPFEPHPLFASFIAAAVKQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=557, Percent_Identity=43.6265709156194, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI28559083, Length=557, Percent_Identity=43.6265709156194, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI221316689, Length=557, Percent_Identity=43.6265709156194, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI148491070, Length=558, Percent_Identity=43.010752688172, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=56.9029850746269, Blast_Score=601, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=34.3185550082102, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6322563, Length=574, Percent_Identity=39.8954703832753, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=39.0158172231986, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=558, Percent_Identity=43.3691756272401, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=42.3459244532803, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_HYPNA (Q0C195)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760498.1
- ProteinModelPortal:   Q0C195
- SMR:   Q0C195
- STRING:   Q0C195
- GeneID:   4289440
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1794
- NMPDR:   fig|228405.5.peg.1727
- TIGR:   HNE_1794
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- PhylomeDB:   Q0C195
- ProtClustDB:   PRK05380
- BioCyc:   HNEP81032:HNE_1794-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59757; Mature: 59757

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 382-382 ACT_SITE 516-516 ACT_SITE 518-518

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYIFITGGVVSSLGKGIASASLGALLQSRGYRVRLRKLDPYLNVDPGTMSPRQHGEVY
CEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
VTDDGAETDLDLGHYERFTGVSARQSDNITTGRIYQRIIEKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHH
DIKEFVLSDPGEGVDFVLCEIGGTVGDIEGLPFFEAIRQLGQELGPQRACFIHLTLLPYI
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHCC
PAAGEMKTKPTQHSVKELRSIGIQPQILLCRCDRPIPVNEKGKIASFCNVRLASVIEARD
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHC
VGHIYDVPMAYHAEGLDSEVLSHFGITDAPPPDLSSWQRIAQTIKNPDGQVTIGLVGKYT
CCCEECCCHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCC
DVPDAYKSVSEALGHGGLANKVKVDIRFVDSEKFDDPDAALEDLDGVHGILLPGGFGERG
CCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCC
AHGKMRVARYARERNMPCFGICYGMQLSVVEAARNLAGIKNASTSEFGPTKEPVVGLMEE
CCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
WTKGNEKVTRDASTDLGGTMRLGAYPARLKEGSMVAQVYGSLEISERHRHRYEVNASYIE
HCCCCCCEECCCCCCCCCEEEECCCHHHHCCCCEEEHHHCCCCHHHHHHHEEECCHHHHH
RLEKAGMIFSGMSPDGRLPEIVELEGHPWFIGVQFHPELKSRPFEPHPLFASFIAAAVKQ
HHHHCCCEEECCCCCCCCCCEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHH
SRLV
HCCC
>Mature Secondary Structure
MIRYIFITGGVVSSLGKGIASASLGALLQSRGYRVRLRKLDPYLNVDPGTMSPRQHGEVY
CEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
VTDDGAETDLDLGHYERFTGVSARQSDNITTGRIYQRIIEKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHH
DIKEFVLSDPGEGVDFVLCEIGGTVGDIEGLPFFEAIRQLGQELGPQRACFIHLTLLPYI
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHCC
PAAGEMKTKPTQHSVKELRSIGIQPQILLCRCDRPIPVNEKGKIASFCNVRLASVIEARD
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHC
VGHIYDVPMAYHAEGLDSEVLSHFGITDAPPPDLSSWQRIAQTIKNPDGQVTIGLVGKYT
CCCEECCCHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCC
DVPDAYKSVSEALGHGGLANKVKVDIRFVDSEKFDDPDAALEDLDGVHGILLPGGFGERG
CCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCC
AHGKMRVARYARERNMPCFGICYGMQLSVVEAARNLAGIKNASTSEFGPTKEPVVGLMEE
CCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
WTKGNEKVTRDASTDLGGTMRLGAYPARLKEGSMVAQVYGSLEISERHRHRYEVNASYIE
HCCCCCCEECCCCCCCCCEEEECCCHHHHCCCCEEEHHHCCCCHHHHHHHEEECCHHHHH
RLEKAGMIFSGMSPDGRLPEIVELEGHPWFIGVQFHPELKSRPFEPHPLFASFIAAAVKQ
HHHHCCCEEECCCCCCCCCCEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHH
SRLV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA