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The map label for this gene is pcm [H]

Identifier: 114800134

GI number: 114800134

Start: 1998332

End: 1998991

Strand: Reverse

Name: pcm [H]

Synonym: HNE_1928

Alternate gene names: 114800134

Gene position: 1998991-1998332 (Counterclockwise)

Preceding gene: 114799382

Following gene: 114797763

Centisome position: 53.95

GC content: 66.06

Gene sequence:

>660_bases
ATGGCAGAGCTGATCGCTGATCCCTTCCGCGCCGCGCGGCTGCTGCTCTCGCTGAGGCAGGAGGGGGTGACTGATCCCAG
CGTTCTGGCGGCCATGGAAACGATCGGCCGGGCGGCGTTTGTGGACGATGCCGGGCTTGAGGCGCTTTCCTTCGAAGATT
CGATCATCCCGATACCTTGTGGCCAGGTGATGTTGCGCCCCTCGGCGACGGGGCATCTGATCCAGGCCATGGGATTGAAG
CGCGGCCAGGAAGGGCGCGTGCTGCTGATCGGTTTTGGTTCGGGCTATATGGCGGCGCTGCTCTCCCAGCTTGCCGCGCA
TGTGTTTGCGGTCGAGCGCTTTGCCCGTCTGACGGAAGAGGGGCGGGTCCGGCTCGCGCGGCTTGGCGTGGAGAATGTGA
CGCTGTCCCATCGTGATGGCCTGCATGGATGGGCCGAGCAGGGCCCATTTGACGCGATCGTGCTGGCGGGCGCCGTTCCC
GCCGTCCCGGAAGGGCTGACCGCGCAGTTGACGCGGGGCGGGGTGCTGGTGGCCCCCATCAATGAGGGGGCTGGGCAGGC
GAATCTTTTGCGCGTGACTGCCTCGGGAAGCGAGCTGGACAGACGGCCGCTGTTTCAATGGGTGCCGCCGCTTATCGAGG
GACGCGCGCAAGTGCTTTGA

Upstream 100 bases:

>100_bases
AAGGCACTGACATCCGGGCAATCTATGACGGCTACGTCTCGATCTCCCCGCTGCATGTGGACTTGACGCATGAGCCGTTC
CTGAAAACGCTGAAGGAGTC

Downstream 100 bases:

>100_bases
TTGCAGGGGGAATCGCCTGCAGGGGTAGTTTGCCAAGGCGCAGCCGCGCCGCTATACGCCTCAAGTTCCCTGTTACCGCC
GCAGAATTTTCTGGAGAATG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MAELIADPFRAARLLLSLRQEGVTDPSVLAAMETIGRAAFVDDAGLEALSFEDSIIPIPCGQVMLRPSATGHLIQAMGLK
RGQEGRVLLIGFGSGYMAALLSQLAAHVFAVERFARLTEEGRVRLARLGVENVTLSHRDGLHGWAEQGPFDAIVLAGAVP
AVPEGLTAQLTRGGVLVAPINEGAGQANLLRVTASGSELDRRPLFQWVPPLIEGRAQVL

Sequences:

>Translated_219_residues
MAELIADPFRAARLLLSLRQEGVTDPSVLAAMETIGRAAFVDDAGLEALSFEDSIIPIPCGQVMLRPSATGHLIQAMGLK
RGQEGRVLLIGFGSGYMAALLSQLAAHVFAVERFARLTEEGRVRLARLGVENVTLSHRDGLHGWAEQGPFDAIVLAGAVP
AVPEGLTAQLTRGGVLVAPINEGAGQANLLRVTASGSELDRRPLFQWVPPLIEGRAQVL
>Mature_218_residues
AELIADPFRAARLLLSLRQEGVTDPSVLAAMETIGRAAFVDDAGLEALSFEDSIIPIPCGQVMLRPSATGHLIQAMGLKR
GQEGRVLLIGFGSGYMAALLSQLAAHVFAVERFARLTEEGRVRLARLGVENVTLSHRDGLHGWAEQGPFDAIVLAGAVPA
VPEGLTAQLTRGGVLVAPINEGAGQANLLRVTASGSELDRRPLFQWVPPLIEGRAQVL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=190, Percent_Identity=38.4210526315789, Blast_Score=124, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23230; Mature: 23099

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELIADPFRAARLLLSLRQEGVTDPSVLAAMETIGRAAFVDDAGLEALSFEDSIIPIPC
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCEEECCCCEEECCC
GQVMLRPSATGHLIQAMGLKRGQEGRVLLIGFGSGYMAALLSQLAAHVFAVERFARLTEE
CCEEECCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVRLARLGVENVTLSHRDGLHGWAEQGPFDAIVLAGAVPAVPEGLTAQLTRGGVLVAPI
CCEEEEEECCCCEEECCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEC
NEGAGQANLLRVTASGSELDRRPLFQWVPPLIEGRAQVL
CCCCCCCEEEEEEECCCCCCCCCHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
AELIADPFRAARLLLSLRQEGVTDPSVLAAMETIGRAAFVDDAGLEALSFEDSIIPIPC
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCEEECCCCEEECCC
GQVMLRPSATGHLIQAMGLKRGQEGRVLLIGFGSGYMAALLSQLAAHVFAVERFARLTEE
CCEEECCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVRLARLGVENVTLSHRDGLHGWAEQGPFDAIVLAGAVPAVPEGLTAQLTRGGVLVAPI
CCEEEEEECCCCEEECCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEC
NEGAGQANLLRVTASGSELDRRPLFQWVPPLIEGRAQVL
CCCCCCCEEEEEEECCCCCCCCCHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA