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The map label for this gene is ykoW [H]

Identifier: 114799436

GI number: 114799436

Start: 59697

End: 61481

Strand: Direct

Name: ykoW [H]

Synonym: HNE_0074

Alternate gene names: 114799436

Gene position: 59697-61481 (Clockwise)

Preceding gene: 114800187

Following gene: 114797180

Centisome position: 1.61

GC content: 63.03

Gene sequence:

>1785_bases
GTGGGCAATGGACTGAAACAGGGAAGACTTGTCGGCTGGTTGACCAGCCGCCTCACCATTCCGGCATTTGCCGCGATGAC
AGGCGCCCTGTTGTCCATTTTCCTGTTTGTTGTGATCGTCGCTCTGGAAGTTGTGTTCCGCATCAGAGGCACAGACGCTC
CGGTCGGTTTTACCGTTCCATTCCCGGTAATCGTGGGCATGTTGATCGGAACAATCGCGATTTCCTCAACTGCCTGTTAC
CTGCTCGCGCGTCCGCTCCAGGGCGTGTTCGTGCGCTTCACCGGCTATATGATGTCGTCCCGCGGCGATGTGGACGCTGA
CCTCCATTCGCTGCAATTTCCGGAGCTGCGCCGTCTCCGCGTCGCCACCACCCGCACTGTGCGCAAGCTGCGGCGCGAGA
ACGACCATCTGCGCAAGATTGCATATCGCGACAGCCGGACGGGCCTTCCCAATGCGCTTGCCGCTGAAAAGCGGATTATC
GAAACGCTGCCAGGCGCCAGTTTTGAGCGGCCCGCGGCATTCATCCTTCTCGACATTGACCGCTTCGCCCACCTGGTAGA
GCAGGTCGGGGCAGGGCATGGCGACATGATGATCCATGCGGCCGCCCAGCGCATTTCAAAAGCGCTCAGCGATGTGCCCG
ATCCGTCCGCGTCTGCATTGCGCGGCTCGATGCTCGCGGCGCTGACGGCAGACCAGTTCACGCTGTTCATGCCCAACGCC
GGGAGCCGGGAACATGTGATCGCGATTGCCCGCGCCATCCGGAACGCCTTCAACGATCCGTTCACGATCCTTGGCCGCCC
GGTCACCATGTCGCTGTCGGGCGGTATCGTGATGGCGCCGGAAGACGCCGACTCGCCGCAGAAGCTGATGCAGAATGCCA
AGCTGGCGCTGCGCCTTGTGCGGAATGAATCGCAGTCGGGTTTCCGTTTCTTCACGCCGCGCCTGACGCGCATCGTGCAG
GGCCGTTACCGGTTTGAGGCAGAGCTGCGCGACGCGGTGGAAAACCGTGAGTTCAAGGCCGTCTTCCAGCCTAAGATCGA
TTTTGCCACCGGCCGCATCGTTGGCGCCGAGGCGCTGGCCCGTTGGCGGCGCTCGAATGGCAAGCTGATTTCGCCGGCGG
CTTTTATTCCGGTGGCGGAGGAAGCGGGCCTGATCGAAGAGATCGGCAAGCAGATCCTGGAAGCGGCCTGTGAAGCAGCC
CGCACCTGGGAGCTGGAAGGCCATGATGTGACCGTGGCGGTCAACGTTTCGCCCAGCCAGTTCCAGCGTAACGACCTGAC
CGAAACCGTCATGGGCGCGCTGCGTCGCTCAGGGCTCCATCCCCGCCTGCTGGAGCTTGAGATCACCGAGAGCATGGCCG
TGTCAGACCCCGCGCGGGTGGCGGAGTTCATCTCGCCGCTGCGGGCGATGGGTACCAAGCTCGCGATTGATGACTTCGGC
ACCGGCCACTCAAACCTCGCCACGCTGACCCAGCTGCCATTCGACGTGTTCAAGATTGACCGGCAATTCGTGTCTGCTCT
GGAGACGGATCGTCAGGCCCCTGCGATTGTCGAGATGATCCTCGCCATGGCCGAAACGCTTGGCCTCAAGACGGTGGCCG
AGGGCGTCGAGACCACCAAGCAGGCAGACTTCCTGCGCCGCCGGGGCTGCACCATGGCGCAGGGCTTCCTCTACAGTCCC
GGCCTGCCCGATCAGGCATTCCTGGAACTGCTGCGCGCCTGGAAGCCGCTGGGCGCCAATGACGAGACGGGCAATCCCGA
CGCCTATCAGGTGCCGCGCCGGTAA

Upstream 100 bases:

>100_bases
TATAAACTGCCGGAGGCGCAACAGCTAGCGCATCTGTGTTCACGGATTCCTAAACATCTAAGGCGCAGTCTTTGCGGCAA
TTACTGAGTTGGGCTTCCGA

Downstream 100 bases:

>100_bases
GTGTTTACGGGCGTGCCTGCCAGTAATGTCTGGCCGGTGTGACGGTAGCCGATCTTCCCTGCCACCTTGCAGGACGCCAG
ATTGCTTGGCTCGATCACAC

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MGNGLKQGRLVGWLTSRLTIPAFAAMTGALLSIFLFVVIVALEVVFRIRGTDAPVGFTVPFPVIVGMLIGTIAISSTACY
LLARPLQGVFVRFTGYMMSSRGDVDADLHSLQFPELRRLRVATTRTVRKLRRENDHLRKIAYRDSRTGLPNALAAEKRII
ETLPGASFERPAAFILLDIDRFAHLVEQVGAGHGDMMIHAAAQRISKALSDVPDPSASALRGSMLAALTADQFTLFMPNA
GSREHVIAIARAIRNAFNDPFTILGRPVTMSLSGGIVMAPEDADSPQKLMQNAKLALRLVRNESQSGFRFFTPRLTRIVQ
GRYRFEAELRDAVENREFKAVFQPKIDFATGRIVGAEALARWRRSNGKLISPAAFIPVAEEAGLIEEIGKQILEAACEAA
RTWELEGHDVTVAVNVSPSQFQRNDLTETVMGALRRSGLHPRLLELEITESMAVSDPARVAEFISPLRAMGTKLAIDDFG
TGHSNLATLTQLPFDVFKIDRQFVSALETDRQAPAIVEMILAMAETLGLKTVAEGVETTKQADFLRRRGCTMAQGFLYSP
GLPDQAFLELLRAWKPLGANDETGNPDAYQVPRR

Sequences:

>Translated_594_residues
MGNGLKQGRLVGWLTSRLTIPAFAAMTGALLSIFLFVVIVALEVVFRIRGTDAPVGFTVPFPVIVGMLIGTIAISSTACY
LLARPLQGVFVRFTGYMMSSRGDVDADLHSLQFPELRRLRVATTRTVRKLRRENDHLRKIAYRDSRTGLPNALAAEKRII
ETLPGASFERPAAFILLDIDRFAHLVEQVGAGHGDMMIHAAAQRISKALSDVPDPSASALRGSMLAALTADQFTLFMPNA
GSREHVIAIARAIRNAFNDPFTILGRPVTMSLSGGIVMAPEDADSPQKLMQNAKLALRLVRNESQSGFRFFTPRLTRIVQ
GRYRFEAELRDAVENREFKAVFQPKIDFATGRIVGAEALARWRRSNGKLISPAAFIPVAEEAGLIEEIGKQILEAACEAA
RTWELEGHDVTVAVNVSPSQFQRNDLTETVMGALRRSGLHPRLLELEITESMAVSDPARVAEFISPLRAMGTKLAIDDFG
TGHSNLATLTQLPFDVFKIDRQFVSALETDRQAPAIVEMILAMAETLGLKTVAEGVETTKQADFLRRRGCTMAQGFLYSP
GLPDQAFLELLRAWKPLGANDETGNPDAYQVPRR
>Mature_593_residues
GNGLKQGRLVGWLTSRLTIPAFAAMTGALLSIFLFVVIVALEVVFRIRGTDAPVGFTVPFPVIVGMLIGTIAISSTACYL
LARPLQGVFVRFTGYMMSSRGDVDADLHSLQFPELRRLRVATTRTVRKLRRENDHLRKIAYRDSRTGLPNALAAEKRIIE
TLPGASFERPAAFILLDIDRFAHLVEQVGAGHGDMMIHAAAQRISKALSDVPDPSASALRGSMLAALTADQFTLFMPNAG
SREHVIAIARAIRNAFNDPFTILGRPVTMSLSGGIVMAPEDADSPQKLMQNAKLALRLVRNESQSGFRFFTPRLTRIVQG
RYRFEAELRDAVENREFKAVFQPKIDFATGRIVGAEALARWRRSNGKLISPAAFIPVAEEAGLIEEIGKQILEAACEAAR
TWELEGHDVTVAVNVSPSQFQRNDLTETVMGALRRSGLHPRLLELEITESMAVSDPARVAEFISPLRAMGTKLAIDDFGT
GHSNLATLTQLPFDVFKIDRQFVSALETDRQAPAIVEMILAMAETLGLKTVAEGVETTKQADFLRRRGCTMAQGFLYSPG
LPDQAFLELLRAWKPLGANDETGNPDAYQVPRR

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=434, Percent_Identity=31.7972350230415, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1787541, Length=437, Percent_Identity=29.5194508009153, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081743, Length=259, Percent_Identity=38.2239382239382, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1790496, Length=255, Percent_Identity=35.6862745098039, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI226510982, Length=349, Percent_Identity=30.3724928366762, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1787055, Length=447, Percent_Identity=28.1879194630872, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081980, Length=261, Percent_Identity=34.8659003831418, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1786507, Length=238, Percent_Identity=33.1932773109244, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1788502, Length=252, Percent_Identity=32.5396825396825, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081845, Length=242, Percent_Identity=33.4710743801653, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87082096, Length=201, Percent_Identity=31.3432835820896, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788849, Length=443, Percent_Identity=25.9593679458239, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787410, Length=233, Percent_Identity=26.6094420600858, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788381, Length=449, Percent_Identity=22.9398663697105, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 65238; Mature: 65107

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNGLKQGRLVGWLTSRLTIPAFAAMTGALLSIFLFVVIVALEVVFRIRGTDAPVGFTVP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FPVIVGMLIGTIAISSTACYLLARPLQGVFVRFTGYMMSSRGDVDADLHSLQFPELRRLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHH
VATTRTVRKLRRENDHLRKIAYRDSRTGLPNALAAEKRIIETLPGASFERPAAFILLDID
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHH
RFAHLVEQVGAGHGDMMIHAAAQRISKALSDVPDPSASALRGSMLAALTADQFTLFMPNA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCEEEECCCC
GSREHVIAIARAIRNAFNDPFTILGRPVTMSLSGGIVMAPEDADSPQKLMQNAKLALRLV
CCHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHH
RNESQSGFRFFTPRLTRIVQGRYRFEAELRDAVENREFKAVFQPKIDFATGRIVGAEALA
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECHHHHH
RWRRSNGKLISPAAFIPVAEEAGLIEEIGKQILEAACEAARTWELEGHDVTVAVNVSPSQ
HHHHCCCCEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEECCHHH
FQRNDLTETVMGALRRSGLHPRLLELEITESMAVSDPARVAEFISPLRAMGTKLAIDDFG
HHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCC
TGHSNLATLTQLPFDVFKIDRQFVSALETDRQAPAIVEMILAMAETLGLKTVAEGVETTK
CCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QADFLRRRGCTMAQGFLYSPGLPDQAFLELLRAWKPLGANDETGNPDAYQVPRR
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GNGLKQGRLVGWLTSRLTIPAFAAMTGALLSIFLFVVIVALEVVFRIRGTDAPVGFTVP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FPVIVGMLIGTIAISSTACYLLARPLQGVFVRFTGYMMSSRGDVDADLHSLQFPELRRLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHH
VATTRTVRKLRRENDHLRKIAYRDSRTGLPNALAAEKRIIETLPGASFERPAAFILLDID
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHH
RFAHLVEQVGAGHGDMMIHAAAQRISKALSDVPDPSASALRGSMLAALTADQFTLFMPNA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCEEEECCCC
GSREHVIAIARAIRNAFNDPFTILGRPVTMSLSGGIVMAPEDADSPQKLMQNAKLALRLV
CCHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHH
RNESQSGFRFFTPRLTRIVQGRYRFEAELRDAVENREFKAVFQPKIDFATGRIVGAEALA
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECHHHHH
RWRRSNGKLISPAAFIPVAEEAGLIEEIGKQILEAACEAARTWELEGHDVTVAVNVSPSQ
HHHHCCCCEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEECCHHH
FQRNDLTETVMGALRRSGLHPRLLELEITESMAVSDPARVAEFISPLRAMGTKLAIDDFG
HHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCC
TGHSNLATLTQLPFDVFKIDRQFVSALETDRQAPAIVEMILAMAETLGLKTVAEGVETTK
CCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QADFLRRRGCTMAQGFLYSPGLPDQAFLELLRAWKPLGANDETGNPDAYQVPRR
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]