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The map label for this gene is glpK [H]

Identifier: 114799418

GI number: 114799418

Start: 1367752

End: 1369233

Strand: Direct

Name: glpK [H]

Synonym: HNE_1335

Alternate gene names: 114799418

Gene position: 1367752-1369233 (Clockwise)

Preceding gene: 114800494

Following gene: 114799350

Centisome position: 36.92

GC content: 68.62

Gene sequence:

>1482_bases
ATGAGCGACTTTATTCTCGTGATCGACGAAGGCACGACCTCGACGCGGGCGATCGTCTATGACCGCGACTTTCGCGAGGT
GGCCCTCGCGCAGGAAGAGGTGGCGCTGGCCTATCCCCGCGATGGCTGGGTGGAGCAGGATGGCGAGGAGATCTGGACGC
GGACGCTGTCGGTCTGCCGGGCGGTGATCGCCAAGGCGGGCGGGGCGGCGCGGATTGCGGCGATCGGCATCACCAACCAG
CGCGAGACGACGCTCGTCTGGGACCGCAAGACCGGCAAGCCGCTGGCGCCGGCCATCATCTGGCAGGACCGGCGCACGGC
GGCGTTCTGTGAAAGCCTCAAGGCGAAGGGCTATGAGAGCGATGTGCAGGCCGAGACCGGCCTGCTGCTGGACCCGTATT
TCTCCGGCACGAAAATAAACTGGGTACTGAATAATGTTCCCGGCGCGCAGGCGCGCGCAGAGGCCGGGGAACTGGCCTTC
GGGACGGTGGACAGTTTTCTGCTGTGGCGGCTGACGGGCGGGACGGTGCATGCCACCGATGTGACGAATGCGTCGCGCAC
GCTGCTCTACCGGCTGGGTATCGGCGGCGAGGGGGGCTGGAGCGCGGCGATGGGCGAGATGCTTGGCGTGCCCGCGGCCA
TGTTGCCCGAGGTGAAGCCGAGCGCGGCGGATTTTGGCACGGCAGGGGCGGCGCTGTTCGGCGCGGCGATCCCGGTGGCG
TCGATCCTGGGGGATCAGCAATCGGCGCTGGTGGGGCAGGGGTGTCTCGCGCCGGGGCAGGCGAAGATCACGTTTGGCAC
GGGCGCGTTCCTGGTGGCGAATACGGGCGCGGCCCGGCCGTTTTCGGAGAACAGATTGCTGGGCACGATGGGCTACGCGC
TGCCCGGCGCCGACGCGATGGCGCTGGAAGGCTCGATCTTCAATGCGGGCACGGTGATCAAATGGCTGCGCGATGATCTG
GGACTTATTCAGAGGGCGGATGAAAGCGCAGCCGCCGCGGCGAGCCTGAAGGATAATGGCGGCGTCTATATGGTGCCCGC
CCTCACCGGGCTTGGCGCGCCGCATTGGGACGCCGAAGCAAGGGGGCTGATCGCCGGGCTGACGCGGGCGGCGACGGGGG
CGCATCTGGTGCGCGCGGGGCTGGAATCGGTGGCCTATCAGACGCATGACCTGCTCGCCGCGTTTGCGGGCGACGGCGCG
CCGATTGGCGAGCTGCGCGTGGATGGCGGCATGGTGGGGAATGACTGGCTGATGCAGTTTCTGGCGAATGTGTGCGCGCT
GCCGGTGCTGCGGCCGGACTATCGCGAGATGACGGCGCTGGGCGCAGCGGCGGCTGCGGCGATGCAGATCGGCTGGCTGA
GCGCCGCCGACTGGGCGGCCCGCGAAGTGCCGGGCAAACGCTTCGAGCCAGATATGAGCGCTGACGCCCGCGCCGCGCTT
CTGAAGGGCTGGCAAACCGCGCTCGGCCGCGCGCTGACCTGA

Upstream 100 bases:

>100_bases
TGGGCGGGCTGCCCATCGGGTTCCAGCTGATCGGGGCGCCGTTTGCGGACAGGACGACGCTGCGCTTCGCATCTCTGCTG
GAAGCTGCGGGGCTGATCGA

Downstream 100 bases:

>100_bases
ATACTTCTCAGGCGCGCAGCCATAAAAAAGGGCGGCCCGGAAGCCGCCCTTTTCATTTTGGGTATACCCGGACCTACATG
TTGCAGGCGGTGTTGAGGAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSDFILVIDEGTTSTRAIVYDRDFREVALAQEEVALAYPRDGWVEQDGEEIWTRTLSVCRAVIAKAGGAARIAAIGITNQ
RETTLVWDRKTGKPLAPAIIWQDRRTAAFCESLKAKGYESDVQAETGLLLDPYFSGTKINWVLNNVPGAQARAEAGELAF
GTVDSFLLWRLTGGTVHATDVTNASRTLLYRLGIGGEGGWSAAMGEMLGVPAAMLPEVKPSAADFGTAGAALFGAAIPVA
SILGDQQSALVGQGCLAPGQAKITFGTGAFLVANTGAARPFSENRLLGTMGYALPGADAMALEGSIFNAGTVIKWLRDDL
GLIQRADESAAAAASLKDNGGVYMVPALTGLGAPHWDAEARGLIAGLTRAATGAHLVRAGLESVAYQTHDLLAAFAGDGA
PIGELRVDGGMVGNDWLMQFLANVCALPVLRPDYREMTALGAAAAAAMQIGWLSAADWAAREVPGKRFEPDMSADARAAL
LKGWQTALGRALT

Sequences:

>Translated_493_residues
MSDFILVIDEGTTSTRAIVYDRDFREVALAQEEVALAYPRDGWVEQDGEEIWTRTLSVCRAVIAKAGGAARIAAIGITNQ
RETTLVWDRKTGKPLAPAIIWQDRRTAAFCESLKAKGYESDVQAETGLLLDPYFSGTKINWVLNNVPGAQARAEAGELAF
GTVDSFLLWRLTGGTVHATDVTNASRTLLYRLGIGGEGGWSAAMGEMLGVPAAMLPEVKPSAADFGTAGAALFGAAIPVA
SILGDQQSALVGQGCLAPGQAKITFGTGAFLVANTGAARPFSENRLLGTMGYALPGADAMALEGSIFNAGTVIKWLRDDL
GLIQRADESAAAAASLKDNGGVYMVPALTGLGAPHWDAEARGLIAGLTRAATGAHLVRAGLESVAYQTHDLLAAFAGDGA
PIGELRVDGGMVGNDWLMQFLANVCALPVLRPDYREMTALGAAAAAAMQIGWLSAADWAAREVPGKRFEPDMSADARAAL
LKGWQTALGRALT
>Mature_492_residues
SDFILVIDEGTTSTRAIVYDRDFREVALAQEEVALAYPRDGWVEQDGEEIWTRTLSVCRAVIAKAGGAARIAAIGITNQR
ETTLVWDRKTGKPLAPAIIWQDRRTAAFCESLKAKGYESDVQAETGLLLDPYFSGTKINWVLNNVPGAQARAEAGELAFG
TVDSFLLWRLTGGTVHATDVTNASRTLLYRLGIGGEGGWSAAMGEMLGVPAAMLPEVKPSAADFGTAGAALFGAAIPVAS
ILGDQQSALVGQGCLAPGQAKITFGTGAFLVANTGAARPFSENRLLGTMGYALPGADAMALEGSIFNAGTVIKWLRDDLG
LIQRADESAAAAASLKDNGGVYMVPALTGLGAPHWDAEARGLIAGLTRAATGAHLVRAGLESVAYQTHDLLAAFAGDGAP
IGELRVDGGMVGNDWLMQFLANVCALPVLRPDYREMTALGAAAAAAMQIGWLSAADWAAREVPGKRFEPDMSADARAALL
KGWQTALGRALT

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=42.6587301587302, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=42.6587301587302, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI42794763, Length=510, Percent_Identity=41.9607843137255, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=39.6414342629482, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=31.3725490196078, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=47.4645030425963, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1790005, Length=491, Percent_Identity=27.2912423625255, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789987, Length=452, Percent_Identity=26.5486725663717, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789168, Length=477, Percent_Identity=25.5765199161426, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=37.5502008032129, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=25.0478011472275, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=27.6666666666667, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=489, Percent_Identity=32.5153374233129, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=40.4715127701375, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24654573, Length=449, Percent_Identity=41.2026726057906, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24655555, Length=508, Percent_Identity=37.007874015748, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24655560, Length=508, Percent_Identity=37.007874015748, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24655570, Length=508, Percent_Identity=37.007874015748, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24655574, Length=508, Percent_Identity=37.007874015748, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24655565, Length=508, Percent_Identity=37.007874015748, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI19922058, Length=531, Percent_Identity=29.9435028248588, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24652871, Length=496, Percent_Identity=29.8387096774194, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24656367, Length=492, Percent_Identity=29.2682926829268, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21357867, Length=504, Percent_Identity=28.5714285714286, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656372, Length=348, Percent_Identity=30.7471264367816, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45552909, Length=270, Percent_Identity=30, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 51622; Mature: 51491

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00933 FGGY_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFILVIDEGTTSTRAIVYDRDFREVALAQEEVALAYPRDGWVEQDGEEIWTRTLSVCR
CCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHH
AVIAKAGGAARIAAIGITNQRETTLVWDRKTGKPLAPAIIWQDRRTAAFCESLKAKGYES
HHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCC
DVQAETGLLLDPYFSGTKINWVLNNVPGAQARAEAGELAFGTVDSFLLWRLTGGTVHATD
CCCCCCCCEECCCCCCCEEEEEEECCCCCHHHCCCCCEEEHHHHHEEEEEEECCEEEEEE
VTNASRTLLYRLGIGGEGGWSAAMGEMLGVPAAMLPEVKPSAADFGTAGAALFGAAIPVA
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
SILGDQQSALVGQGCLAPGQAKITFGTGAFLVANTGAARPFSENRLLGTMGYALPGADAM
HHHCCCHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCE
ALEGSIFNAGTVIKWLRDDLGLIQRADESAAAAASLKDNGGVYMVPALTGLGAPHWDAEA
EECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEHHCCCCCCCCCHH
RGLIAGLTRAATGAHLVRAGLESVAYQTHDLLAAFAGDGAPIGELRVDGGMVGNDWLMQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
LANVCALPVLRPDYREMTALGAAAAAAMQIGWLSAADWAAREVPGKRFEPDMSADARAAL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHH
LKGWQTALGRALT
HHHHHHHHHCCCC
>Mature Secondary Structure 
SDFILVIDEGTTSTRAIVYDRDFREVALAQEEVALAYPRDGWVEQDGEEIWTRTLSVCR
CCEEEEEECCCCCCEEEEEECCHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHH
AVIAKAGGAARIAAIGITNQRETTLVWDRKTGKPLAPAIIWQDRRTAAFCESLKAKGYES
HHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCC
DVQAETGLLLDPYFSGTKINWVLNNVPGAQARAEAGELAFGTVDSFLLWRLTGGTVHATD
CCCCCCCCEECCCCCCCEEEEEEECCCCCHHHCCCCCEEEHHHHHEEEEEEECCEEEEEE
VTNASRTLLYRLGIGGEGGWSAAMGEMLGVPAAMLPEVKPSAADFGTAGAALFGAAIPVA
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
SILGDQQSALVGQGCLAPGQAKITFGTGAFLVANTGAARPFSENRLLGTMGYALPGADAM
HHHCCCHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCE
ALEGSIFNAGTVIKWLRDDLGLIQRADESAAAAASLKDNGGVYMVPALTGLGAPHWDAEA
EECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEHHCCCCCCCCCHH
RGLIAGLTRAATGAHLVRAGLESVAYQTHDLLAAFAGDGAPIGELRVDGGMVGNDWLMQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
LANVCALPVLRPDYREMTALGAAAAAAMQIGWLSAADWAAREVPGKRFEPDMSADARAAL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHH
LKGWQTALGRALT
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA