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The map label for this gene is gatA2 [H]

Identifier: 114800494

GI number: 114800494

Start: 1366391

End: 1367755

Strand: Direct

Name: gatA2 [H]

Synonym: HNE_1334

Alternate gene names: 114800494

Gene position: 1366391-1367755 (Clockwise)

Preceding gene: 114798831

Following gene: 114799418

Centisome position: 36.88

GC content: 65.57

Gene sequence:

>1365_bases
ATGGCCGCAAAGACCGGGATTGATCTGGCAAAGGCTGGCGCGCGGGAGACGGCCGAGGCTGTTCGCGCCGGGGCCATATC
CGCGCTGGAGGCGGCCGACGCCGCCATTGCCCGCATCGAAGCCGTTGATGGCGCAATCAACGCGGTCGTCGTGCGCGATT
TCGAACGGGCACGGGCAGCGGCGAAAGCCGTGGACGCGAGCCGGATGCCGGGCGACATGCGGCGGCTTCTGGGTGTGCCG
ATGACAGTGAAGGAGTCCAATGATGTGGCAGGCCTGCCCTCCACTTGGGGGTTTGAGTCGTTCAAGGATGTGGACGCGTC
GCGAGATGCTGTGGTTGTGACGCGCCTCAAGGCTGAAGGCGCAATCATCCTTGGCAAGACGAATGTTCCCGTCGCATTGG
GTGACTGGCAGAGCGTGAACCCGGTCTATGGGCGCACGGTGAACCCGTTTGATCATACGCGCTCGCCAGGGGGATCTTCC
GGCGGGGCGGCGGCGGCGCTGGCGACCGGGATGGTGCCTCTGGAGATCGGCTCGGACATCGGCGGGTCTATCCGCTTTCC
GGCGCATATGTGCGGCGTGTTCGGGCACAAGCCGAGCTGGGGCATTGTGCCCCAGAGAGGGCATCTCTTTCCGGGGACGG
ATGGGTGTGATGCGCCCCTTGCTGTGGTGGGGCCGATGGCGCGTAATGTGGGCGACCTGATTGCCGCGCTTGAGGTGATC
GCCGGGCCGGAGGCTGGCAGCGGGATGCGTCTGGACCTGCCGGCGCCGCGCGCGGGCGGGCTCGGCGGTTTCCGCGTCCG
GGTGCTGCGCGAGATGCCAGGGGTGCCGGTGGACAGAGATACGGGAAACGCGCTGGAGCGCTTTGCGGGGCAGTTGCGCG
CGGCAGGCGTGGTGGTGAGCGAGGGGAGCGAGGGTTTACCGGATCTCGCCGAGATGGTGCCCACTTATGTGCGCATGCTG
ATGACGGTGATCTCGCGCGGCGAACCTTCGGCCACGCCGATCAGCGCCCATGACTGGATGAATTTGCTGGATTATCAGCT
CGCCATTACACGGCAGTGGTCGGCCTTTTTTGAGGGCGCCGATATTTTGCTCTCGCCTGCTTTCTCGACACCGGCCTTTC
CGCACAAGGACGAGCCTGATTGGGGCAAGCGCACCCTGGATATTGACGGGCTGACACTGCCCTATGGGGCTCAGCTCGCC
TGGGCGTCGATTGCGACCTTTGCGGGGCTTCCGTCTACCTGTGTACCGGTTGCCAAGAGCGTGGGCGGGCTGCCCATCGG
GTTCCAGCTGATCGGGGCGCCGTTTGCGGACAGGACGACGCTGCGCTTCGCATCTCTGCTGGAAGCTGCGGGGCTGATCG
AATGA

Upstream 100 bases:

>100_bases
TCCTTCCAGTGGAGCCCGCAACAGGCGATTGCAGGCAGGTCGCTTTGCGTTGAGCTTCTGAACGGGCGTGCTAGGCTCGC
CCGAAAGAGGGAGAGAACGC

Downstream 100 bases:

>100_bases
GCGACTTTATTCTCGTGATCGACGAAGGCACGACCTCGACGCGGGCGATCGTCTATGACCGCGACTTTCGCGAGGTGGCC
CTCGCGCAGGAAGAGGTGGC

Product: amidase family protein

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAAKTGIDLAKAGARETAEAVRAGAISALEAADAAIARIEAVDGAINAVVVRDFERARAAAKAVDASRMPGDMRRLLGVP
MTVKESNDVAGLPSTWGFESFKDVDASRDAVVVTRLKAEGAIILGKTNVPVALGDWQSVNPVYGRTVNPFDHTRSPGGSS
GGAAAALATGMVPLEIGSDIGGSIRFPAHMCGVFGHKPSWGIVPQRGHLFPGTDGCDAPLAVVGPMARNVGDLIAALEVI
AGPEAGSGMRLDLPAPRAGGLGGFRVRVLREMPGVPVDRDTGNALERFAGQLRAAGVVVSEGSEGLPDLAEMVPTYVRML
MTVISRGEPSATPISAHDWMNLLDYQLAITRQWSAFFEGADILLSPAFSTPAFPHKDEPDWGKRTLDIDGLTLPYGAQLA
WASIATFAGLPSTCVPVAKSVGGLPIGFQLIGAPFADRTTLRFASLLEAAGLIE

Sequences:

>Translated_454_residues
MAAKTGIDLAKAGARETAEAVRAGAISALEAADAAIARIEAVDGAINAVVVRDFERARAAAKAVDASRMPGDMRRLLGVP
MTVKESNDVAGLPSTWGFESFKDVDASRDAVVVTRLKAEGAIILGKTNVPVALGDWQSVNPVYGRTVNPFDHTRSPGGSS
GGAAAALATGMVPLEIGSDIGGSIRFPAHMCGVFGHKPSWGIVPQRGHLFPGTDGCDAPLAVVGPMARNVGDLIAALEVI
AGPEAGSGMRLDLPAPRAGGLGGFRVRVLREMPGVPVDRDTGNALERFAGQLRAAGVVVSEGSEGLPDLAEMVPTYVRML
MTVISRGEPSATPISAHDWMNLLDYQLAITRQWSAFFEGADILLSPAFSTPAFPHKDEPDWGKRTLDIDGLTLPYGAQLA
WASIATFAGLPSTCVPVAKSVGGLPIGFQLIGAPFADRTTLRFASLLEAAGLIE
>Mature_453_residues
AAKTGIDLAKAGARETAEAVRAGAISALEAADAAIARIEAVDGAINAVVVRDFERARAAAKAVDASRMPGDMRRLLGVPM
TVKESNDVAGLPSTWGFESFKDVDASRDAVVVTRLKAEGAIILGKTNVPVALGDWQSVNPVYGRTVNPFDHTRSPGGSSG
GAAAALATGMVPLEIGSDIGGSIRFPAHMCGVFGHKPSWGIVPQRGHLFPGTDGCDAPLAVVGPMARNVGDLIAALEVIA
GPEAGSGMRLDLPAPRAGGLGGFRVRVLREMPGVPVDRDTGNALERFAGQLRAAGVVVSEGSEGLPDLAEMVPTYVRMLM
TVISRGEPSATPISAHDWMNLLDYQLAITRQWSAFFEGADILLSPAFSTPAFPHKDEPDWGKRTLDIDGLTLPYGAQLAW
ASIATFAGLPSTCVPVAKSVGGLPIGFQLIGAPFADRTTLRFASLLEAAGLIE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=482, Percent_Identity=29.4605809128631, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=44.5161290322581, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI222831590, Length=252, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=29.938900203666, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17538252, Length=227, Percent_Identity=33.4801762114537, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=232, Percent_Identity=34.051724137931, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=164, Percent_Identity=41.4634146341463, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=458, Percent_Identity=26.4192139737991, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=210, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=183, Percent_Identity=35.5191256830601, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=183, Percent_Identity=35.5191256830601, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=217, Percent_Identity=31.3364055299539, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=406, Percent_Identity=28.8177339901478, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24644968, Length=497, Percent_Identity=28.5714285714286, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=29.8597194388778, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21356731, Length=248, Percent_Identity=38.3064516129032, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24648435, Length=487, Percent_Identity=29.5687885010267, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24648437, Length=487, Percent_Identity=29.5687885010267, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI45550774, Length=487, Percent_Identity=29.5687885010267, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24652985, Length=495, Percent_Identity=27.4747474747475, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922090, Length=495, Percent_Identity=27.4747474747475, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652981, Length=495, Percent_Identity=27.4747474747475, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652983, Length=495, Percent_Identity=27.4747474747475, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648441, Length=412, Percent_Identity=30.0970873786408, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648439, Length=412, Percent_Identity=30.0970873786408, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648113, Length=467, Percent_Identity=27.4089935760171, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 47220; Mature: 47088

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTGIDLAKAGARETAEAVRAGAISALEAADAAIARIEAVDGAINAVVVRDFERARAA
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
AKAVDASRMPGDMRRLLGVPMTVKESNDVAGLPSTWGFESFKDVDASRDAVVVTRLKAEG
HHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEEECCC
AIILGKTNVPVALGDWQSVNPVYGRTVNPFDHTRSPGGSSGGAAAALATGMVPLEIGSDI
EEEEECCCCCEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHCCEEEECCCCC
GGSIRFPAHMCGVFGHKPSWGIVPQRGHLFPGTDGCDAPLAVVGPMARNVGDLIAALEVI
CCCEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGPEAGSGMRLDLPAPRAGGLGGFRVRVLREMPGVPVDRDTGNALERFAGQLRAAGVVVS
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEE
EGSEGLPDLAEMVPTYVRMLMTVISRGEPSATPISAHDWMNLLDYQLAITRQWSAFFEGA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DILLSPAFSTPAFPHKDEPDWGKRTLDIDGLTLPYGAQLAWASIATFAGLPSTCVPVAKS
CEEEECCCCCCCCCCCCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHH
VGGLPIGFQLIGAPFADRTTLRFASLLEAAGLIE
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAKTGIDLAKAGARETAEAVRAGAISALEAADAAIARIEAVDGAINAVVVRDFERARAA
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
AKAVDASRMPGDMRRLLGVPMTVKESNDVAGLPSTWGFESFKDVDASRDAVVVTRLKAEG
HHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEEECCC
AIILGKTNVPVALGDWQSVNPVYGRTVNPFDHTRSPGGSSGGAAAALATGMVPLEIGSDI
EEEEECCCCCEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHCCEEEECCCCC
GGSIRFPAHMCGVFGHKPSWGIVPQRGHLFPGTDGCDAPLAVVGPMARNVGDLIAALEVI
CCCEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGPEAGSGMRLDLPAPRAGGLGGFRVRVLREMPGVPVDRDTGNALERFAGQLRAAGVVVS
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEE
EGSEGLPDLAEMVPTYVRMLMTVISRGEPSATPISAHDWMNLLDYQLAITRQWSAFFEGA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DILLSPAFSTPAFPHKDEPDWGKRTLDIDGLTLPYGAQLAWASIATFAGLPSTCVPVAKS
CEEEECCCCCCCCCCCCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHH
VGGLPIGFQLIGAPFADRTTLRFASLLEAAGLIE
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]