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The map label for this gene is mhpC [H]

Identifier: 114799337

GI number: 114799337

Start: 3529672

End: 3530499

Strand: Direct

Name: mhpC [H]

Synonym: HNE_3382

Alternate gene names: 114799337

Gene position: 3529672-3530499 (Clockwise)

Preceding gene: 114798760

Following gene: 114798838

Centisome position: 95.27

GC content: 65.34

Gene sequence:

>828_bases
ATGGCCCGCATACGCGCCAACGGGGTTGAACTTGAATACGAAACGATGGGCAATCCCGGCGATCCGGCCGTCCTGCTGAT
CATGGGCTTTGCCTTTCAGATGACGCGCTGGCCCGAAGCCTTCCGGCGCAGCCTGGCGGAGGCCGGCTTCCACGTTATCT
GGTTCGATAACCGCGACATTGGACTTTCCGCCGAGATGGGCGGCCTCGCCACCTACACGCTGAATGACCTGGCTGCCGAT
GCCGCCGGGCTGCTTGATGCCCTCGGCATTGCGCGGGCCCATATTGTCGGCATGTCGATGGGCGGCATGATCGCCCAGCT
GATAGCGCTCGATCATCCGCGCAAGGTGGACAAGCTCGTCGCCATGATGACGTCGTCTGGCGCGCCAAACCTGCCGCCAC
CGACGCAGGAGGCGCTTGCCGCGCTGACCGCGATGCCGATGCAGCGCACCCCCGAAGCCATTGCGGACATCGCAGTGAAA
GCCCAGCATGCCATCGGCTCCGCACCGAATTTGCGCAACAGCGCCGAGGTGATCCGCCAGAACGCCATCGACGATTTCCG
CCGCTCCGACCGGCCTTTCGGCGTCATCCGTCAGTTCACGGCTATTCAAGCCCAGCCCCGCTGGCATGAACGCCTCGGCA
GCATCACGCGGCCCACGCTCGTTCTCCACGGCGCAGACGACCCGCTCGTCCGCCCGGCGGCCGGCGAAGACATCGCCCGG
CGCATCCCCGGCGCCCGCTTCAAGGCGTTCCCAGGCTGGGGCCATGATCTCGCCGACGCCATGTCAGAAACGCTGGCAAA
CGAGATCACGGCCTTCCTGAAGGCATAA

Upstream 100 bases:

>100_bases
ACGGGGCGGCGTTTTTCTTTGCCTCCACGGCATGCTTGATCTTGGCGTCACCGCCCCTACTGTCCGCCCCGAAGCGGCAA
CAGGACCGGGGGGAAGTACT

Downstream 100 bases:

>100_bases
TCCGCTAGCGGATGGCTACCGGATTGACGATGGCCTGCACCGCCCCCTCAAGGCGCGGCGCGCCGAGCGTGAAGAAGAAC
TCGGTCGCCCCGTCAGCGGC

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MARIRANGVELEYETMGNPGDPAVLLIMGFAFQMTRWPEAFRRSLAEAGFHVIWFDNRDIGLSAEMGGLATYTLNDLAAD
AAGLLDALGIARAHIVGMSMGGMIAQLIALDHPRKVDKLVAMMTSSGAPNLPPPTQEALAALTAMPMQRTPEAIADIAVK
AQHAIGSAPNLRNSAEVIRQNAIDDFRRSDRPFGVIRQFTAIQAQPRWHERLGSITRPTLVLHGADDPLVRPAAGEDIAR
RIPGARFKAFPGWGHDLADAMSETLANEITAFLKA

Sequences:

>Translated_275_residues
MARIRANGVELEYETMGNPGDPAVLLIMGFAFQMTRWPEAFRRSLAEAGFHVIWFDNRDIGLSAEMGGLATYTLNDLAAD
AAGLLDALGIARAHIVGMSMGGMIAQLIALDHPRKVDKLVAMMTSSGAPNLPPPTQEALAALTAMPMQRTPEAIADIAVK
AQHAIGSAPNLRNSAEVIRQNAIDDFRRSDRPFGVIRQFTAIQAQPRWHERLGSITRPTLVLHGADDPLVRPAAGEDIAR
RIPGARFKAFPGWGHDLADAMSETLANEITAFLKA
>Mature_274_residues
ARIRANGVELEYETMGNPGDPAVLLIMGFAFQMTRWPEAFRRSLAEAGFHVIWFDNRDIGLSAEMGGLATYTLNDLAADA
AGLLDALGIARAHIVGMSMGGMIAQLIALDHPRKVDKLVAMMTSSGAPNLPPPTQEALAALTAMPMQRTPEAIADIAVKA
QHAIGSAPNLRNSAEVIRQNAIDDFRRSDRPFGVIRQFTAIQAQPRWHERLGSITRPTLVLHGADDPLVRPAAGEDIARR
IPGARFKAFPGWGHDLADAMSETLANEITAFLKA

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=259, Percent_Identity=28.957528957529, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 29723; Mature: 29591

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIRANGVELEYETMGNPGDPAVLLIMGFAFQMTRWPEAFRRSLAEAGFHVIWFDNRDI
CCCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GLSAEMGGLATYTLNDLAADAAGLLDALGIARAHIVGMSMGGMIAQLIALDHPRKVDKLV
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AMMTSSGAPNLPPPTQEALAALTAMPMQRTPEAIADIAVKAQHAIGSAPNLRNSAEVIRQ
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NAIDDFRRSDRPFGVIRQFTAIQAQPRWHERLGSITRPTLVLHGADDPLVRPAAGEDIAR
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHH
RIPGARFKAFPGWGHDLADAMSETLANEITAFLKA
HCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARIRANGVELEYETMGNPGDPAVLLIMGFAFQMTRWPEAFRRSLAEAGFHVIWFDNRDI
CCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GLSAEMGGLATYTLNDLAADAAGLLDALGIARAHIVGMSMGGMIAQLIALDHPRKVDKLV
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AMMTSSGAPNLPPPTQEALAALTAMPMQRTPEAIADIAVKAQHAIGSAPNLRNSAEVIRQ
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NAIDDFRRSDRPFGVIRQFTAIQAQPRWHERLGSITRPTLVLHGADDPLVRPAAGEDIAR
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHH
RIPGARFKAFPGWGHDLADAMSETLANEITAFLKA
HCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA