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The map label for this gene is ada [H]

Identifier: 114798838

GI number: 114798838

Start: 3532547

End: 3533515

Strand: Direct

Name: ada [H]

Synonym: HNE_3385

Alternate gene names: 114798838

Gene position: 3532547-3533515 (Clockwise)

Preceding gene: 114799337

Following gene: 114800513

Centisome position: 95.34

GC content: 68.63

Gene sequence:

>969_bases
TTGCAGGAAAGTGTAAACACCGCCCTTCACCTTGCGGCAAGAATCGGGTGGCGCCGCGTCACCCCCTCCGGCAAACTCCC
TTTCATGGCTGACACACTCTTCCCCCTCGACGACCGCGCCCGCGCCTATGACCGTATGGCAAAAGCCCTCGAATGGCTGG
GCGACAGCTGGCAGGACTGGCCAGCTCTTGACGAAGCAGCCCGCGCGATGGGCCTCTCGCCGCATCATTTCCAGCGCGAA
TTTACCCGCTGGGCGGGCATCAGCCCCAAACAGTTCCAGGGCGCCCTCGCCCATGGCGCCGCCGGGGATCTCCTGCGCCA
GGGCGCCAGCGTGCTCGACGCCAGCCTCGAAACCGGCCTCTCCGGCCCCGGCCGCCTGCATGACCTCTTCATCGCTCACG
AGGGCCTCACCCCCGGCGAAGCCAAATCCGGCGGCAAGGGCGCAGAACTCACCCTTGGCAAGGCCCCCACGCCGTTCGGC
ATGGGCGCCTGGCTGATCGGGCCGCGCGGCCTTATCGCACTCGGCTTCATTGATGAGAACGCCTCCCCCCGCACCGGCTT
CGAACATCAGGGTCGCAGCGAGGACGCCGCCTTCGCAAACCTCGCCGCCCGCTACCCCGCCGCTTCACTTCGCCGTGAGG
ACGCCGCCGCTGAGCGCATGGCCCGCGACGTGTTCGAGGACGGCCAGCCCCTGCCCGTCTGCCTCTATGGCACGCCCTTC
CGACGGCAGGTCTGGCGCGCCCTGCTGGAGATTCCCGCCGGCCAGACGCGCACTTACGGGCATCTTGCCGCCGCCATCGA
GGCGCCCAAGGCCGCCCGCGCGGTCGGCGCGGCGGTCGGGGCAAACCCCGTCAGCTGGTTTATCCCCTGCCACCGGGCCC
TTGCAGCCGATGGCCGCCTGCATAACTATCATTGGGGTGTGGCGCGCAAACGCGCCATGCTGGTATACGAACGCGCTCAT
CTGGCCTGA

Upstream 100 bases:

>100_bases
GCAGGGTACTCGTGCAGGATGATTGTGCGGGCGTCCGCCATGTTGACCGTCTAGCCTCCCGAAGTCTGTTTTTGGGAGCG
CACATTGCGCGCGTTCCTGT

Downstream 100 bases:

>100_bases
TGCTTACAGGCTGGCCAGTCTCGGGCGCCCGGAACCTGCATTGATTCTGGCACCGATCCTGGCACTGAAGCGGACCTGAC
CCATGTGGCTCTATTTTCTT

Product: ADA regulatory protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQESVNTALHLAARIGWRRVTPSGKLPFMADTLFPLDDRARAYDRMAKALEWLGDSWQDWPALDEAARAMGLSPHHFQRE
FTRWAGISPKQFQGALAHGAAGDLLRQGASVLDASLETGLSGPGRLHDLFIAHEGLTPGEAKSGGKGAELTLGKAPTPFG
MGAWLIGPRGLIALGFIDENASPRTGFEHQGRSEDAAFANLAARYPAASLRREDAAAERMARDVFEDGQPLPVCLYGTPF
RRQVWRALLEIPAGQTRTYGHLAAAIEAPKAARAVGAAVGANPVSWFIPCHRALAADGRLHNYHWGVARKRAMLVYERAH
LA

Sequences:

>Translated_322_residues
MQESVNTALHLAARIGWRRVTPSGKLPFMADTLFPLDDRARAYDRMAKALEWLGDSWQDWPALDEAARAMGLSPHHFQRE
FTRWAGISPKQFQGALAHGAAGDLLRQGASVLDASLETGLSGPGRLHDLFIAHEGLTPGEAKSGGKGAELTLGKAPTPFG
MGAWLIGPRGLIALGFIDENASPRTGFEHQGRSEDAAFANLAARYPAASLRREDAAAERMARDVFEDGQPLPVCLYGTPF
RRQVWRALLEIPAGQTRTYGHLAAAIEAPKAARAVGAAVGANPVSWFIPCHRALAADGRLHNYHWGVARKRAMLVYERAH
LA
>Mature_322_residues
MQESVNTALHLAARIGWRRVTPSGKLPFMADTLFPLDDRARAYDRMAKALEWLGDSWQDWPALDEAARAMGLSPHHFQRE
FTRWAGISPKQFQGALAHGAAGDLLRQGASVLDASLETGLSGPGRLHDLFIAHEGLTPGEAKSGGKGAELTLGKAPTPFG
MGAWLIGPRGLIALGFIDENASPRTGFEHQGRSEDAAFANLAARYPAASLRREDAAAERMARDVFEDGQPLPVCLYGTPF
RRQVWRALLEIPAGQTRTYGHLAAAIEAPKAARAVGAAVGANPVSWFIPCHRALAADGRLHNYHWGVARKRAMLVYERAH
LA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=79, Percent_Identity=45.5696202531646, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788542, Length=265, Percent_Identity=32.0754716981132, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1787596, Length=87, Percent_Identity=49.4252873563218, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=42.8571428571429, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=42.8571428571429, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=103, Percent_Identity=36.8932038834951, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137554, Length=128, Percent_Identity=36.71875, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 34907; Mature: 34907

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQESVNTALHLAARIGWRRVTPSGKLPFMADTLFPLDDRARAYDRMAKALEWLGDSWQDW
CCCHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PALDEAARAMGLSPHHFQREFTRWAGISPKQFQGALAHGAAGDLLRQGASVLDASLETGL
CCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
SGPGRLHDLFIAHEGLTPGEAKSGGKGAELTLGKAPTPFGMGAWLIGPRGLIALGFIDEN
CCCCHHHEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEECCC
ASPRTGFEHQGRSEDAAFANLAARYPAASLRREDAAAERMARDVFEDGQPLPVCLYGTPF
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
RRQVWRALLEIPAGQTRTYGHLAAAIEAPKAARAVGAAVGANPVSWFIPCHRALAADGRL
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCC
HNYHWGVARKRAMLVYERAHLA
CCEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQESVNTALHLAARIGWRRVTPSGKLPFMADTLFPLDDRARAYDRMAKALEWLGDSWQDW
CCCHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PALDEAARAMGLSPHHFQREFTRWAGISPKQFQGALAHGAAGDLLRQGASVLDASLETGL
CCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
SGPGRLHDLFIAHEGLTPGEAKSGGKGAELTLGKAPTPFGMGAWLIGPRGLIALGFIDEN
CCCCHHHEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEECCC
ASPRTGFEHQGRSEDAAFANLAARYPAASLRREDAAAERMARDVFEDGQPLPVCLYGTPF
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
RRQVWRALLEIPAGQTRTYGHLAAAIEAPKAARAVGAAVGANPVSWFIPCHRALAADGRL
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCC
HNYHWGVARKRAMLVYERAHLA
CCEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]