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The map label for this gene is glyA

Identifier: 114799314

GI number: 114799314

Start: 2133424

End: 2134731

Strand: Reverse

Name: glyA

Synonym: HNE_2060

Alternate gene names: 114799314

Gene position: 2134731-2133424 (Counterclockwise)

Preceding gene: 114797766

Following gene: 114797364

Centisome position: 57.62

GC content: 64.6

Gene sequence:

>1308_bases
ATGGCAGATTCGGCGCATGTTCCAGAAACGCTGGCCGGGGGCTTTTTCTCCGTTGGTCTTGCAGAGCGCGACCCCGAGCT
GGCCGCTGCGATCAACCAGGAAGCCACCCGCCAGCAGCACCAGATCGAGCTGATCGCCTCGGAGAACATCGTCTCCCGCG
CCGTGCTCGAAGCCCAAGGCTCGATCCTCACCAACAAATACGCCGAAGGCTATCCCGGCAAACGCTACTATGGCGGCTGC
GAATTCGTCGACATCGCTGAGGAACTCGCGATCGAGCGCGCCAAGAAGCTGTTCAATTGCGGCTTTGCCAACGTCCAGCC
AAACTCCGGCAGCCAGGCCAACCAGGGCGTCTTCCAGGCGGTCCTGAAGCCCGGCGACACCATCCTCGGCATGAGCCTCG
CCGCCGGTGGCCACCTCACCCACGGCGCCAAGCCAAACCAGTCCGGAAAATGGTTCAACGCTGTCCAGTATGGCGTGCGC
CCGGAAGACCACCTGATCGACTTTGATGAGGTTGAGCGCCTTGCCCGCGCCCACCGCCCGCAGATGATCATCGCCGGTGG
CTCTGCCTATCCGCGCCAGATTGACTTCAAACGCTTCCGCGAGATTGCCGATGATGTCGGCGCCATCTTCCTGGTCGACA
TGGCCCACTTTGCTGGTCTCGTCGCCGGCGGCGCCCATCCCAACCCGCTCGACCATTGCCACATTGCCACCACCACGACG
CACAAGACCCTTCGGGGCCCGCGCGGCGGCATGATCCTCACCAATGACGAAGCTCTCGCCAAGAAGATCAACTCGGCCAT
CTTCCCCGGCATCCAGGGCGGTCCCCTAATGCATGTCATCGCCGGCAAGGCGGTCGCCTTTGGCGAGGCGCTGATGCCGG
AATTCAAGACCTATGTTGAGCAGGTCGTCTCCAACGCCCGCGCCATGGCCGCAGCCTGCCGCACGGCGGGCCTCGATGTC
GTCTCCGATGGCACCGACACCCACCTCGCGCTGATTGACCTGCGCCCCAAAGGCGTCACCGGCCGCGACGCCGAAGCCGC
TCTCGAGCGCGCCTACATCACCTGCAATAAGAACGGCATCCCGTTCGATCCCGCCCCGCCGACGGTCACCTCGGGCATCC
GGGTCGGCTCGCCTGCGGGCACCACGCGCGGCTTCCGCGAGGAAGAGTTCATCCAGATCGGCACCTGGATCGGCGAGATC
GTAGACGCTCTGGCCAATGGCAACAGCGACGCGGTTGAAGCCCGCGTCCGCGAAGAGGTGAAGGCGCTCACGGCGCGTTT
CCCCATCTATGAGGGGCTGGGGGGCTGA

Upstream 100 bases:

>100_bases
TTCTTCCACTCTAGTTGAATTTCTGTAGAACTTCGAGTCGCAGTTGCCGAGCCTGCCTGCACCGCGTAAACAGCCCCAGA
CCGTCGCAAAGGAGGCTCCC

Downstream 100 bases:

>100_bases
TCGGCTTTGCGCTGTCCATTCTGCGGTTCTGACAATACATCCGTAAAAGACAGCCGCGCCGCTGAAGACGATACCGCTGT
GCGCCGCCGCCGCGTCTGTG

Product: serine hydroxymethyl transferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MADSAHVPETLAGGFFSVGLAERDPELAAAINQEATRQQHQIELIASENIVSRAVLEAQGSILTNKYAEGYPGKRYYGGC
EFVDIAEELAIERAKKLFNCGFANVQPNSGSQANQGVFQAVLKPGDTILGMSLAAGGHLTHGAKPNQSGKWFNAVQYGVR
PEDHLIDFDEVERLARAHRPQMIIAGGSAYPRQIDFKRFREIADDVGAIFLVDMAHFAGLVAGGAHPNPLDHCHIATTTT
HKTLRGPRGGMILTNDEALAKKINSAIFPGIQGGPLMHVIAGKAVAFGEALMPEFKTYVEQVVSNARAMAAACRTAGLDV
VSDGTDTHLALIDLRPKGVTGRDAEAALERAYITCNKNGIPFDPAPPTVTSGIRVGSPAGTTRGFREEEFIQIGTWIGEI
VDALANGNSDAVEARVREEVKALTARFPIYEGLGG

Sequences:

>Translated_435_residues
MADSAHVPETLAGGFFSVGLAERDPELAAAINQEATRQQHQIELIASENIVSRAVLEAQGSILTNKYAEGYPGKRYYGGC
EFVDIAEELAIERAKKLFNCGFANVQPNSGSQANQGVFQAVLKPGDTILGMSLAAGGHLTHGAKPNQSGKWFNAVQYGVR
PEDHLIDFDEVERLARAHRPQMIIAGGSAYPRQIDFKRFREIADDVGAIFLVDMAHFAGLVAGGAHPNPLDHCHIATTTT
HKTLRGPRGGMILTNDEALAKKINSAIFPGIQGGPLMHVIAGKAVAFGEALMPEFKTYVEQVVSNARAMAAACRTAGLDV
VSDGTDTHLALIDLRPKGVTGRDAEAALERAYITCNKNGIPFDPAPPTVTSGIRVGSPAGTTRGFREEEFIQIGTWIGEI
VDALANGNSDAVEARVREEVKALTARFPIYEGLGG
>Mature_434_residues
ADSAHVPETLAGGFFSVGLAERDPELAAAINQEATRQQHQIELIASENIVSRAVLEAQGSILTNKYAEGYPGKRYYGGCE
FVDIAEELAIERAKKLFNCGFANVQPNSGSQANQGVFQAVLKPGDTILGMSLAAGGHLTHGAKPNQSGKWFNAVQYGVRP
EDHLIDFDEVERLARAHRPQMIIAGGSAYPRQIDFKRFREIADDVGAIFLVDMAHFAGLVAGGAHPNPLDHCHIATTTTH
KTLRGPRGGMILTNDEALAKKINSAIFPGIQGGPLMHVIAGKAVAFGEALMPEFKTYVEQVVSNARAMAAACRTAGLDVV
SDGTDTHLALIDLRPKGVTGRDAEAALERAYITCNKNGIPFDPAPPTVTSGIRVGSPAGTTRGFREEEFIQIGTWIGEIV
DALANGNSDAVEARVREEVKALTARFPIYEGLGG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=407, Percent_Identity=47.4201474201474, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI261862350, Length=407, Percent_Identity=47.4201474201474, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI261862348, Length=407, Percent_Identity=47.4201474201474, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI19923315, Length=407, Percent_Identity=47.4201474201474, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI22547186, Length=456, Percent_Identity=43.859649122807, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI261862346, Length=407, Percent_Identity=46.4373464373464, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI22547189, Length=439, Percent_Identity=41.4578587699317, Blast_Score=302, Evalue=4e-82,
Organism=Escherichia coli, GI1788902, Length=415, Percent_Identity=58.0722891566265, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI25144732, Length=423, Percent_Identity=44.9172576832151, Blast_Score=343, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=423, Percent_Identity=44.9172576832151, Blast_Score=343, Evalue=9e-95,
Organism=Saccharomyces cerevisiae, GI6323087, Length=422, Percent_Identity=44.7867298578199, Blast_Score=345, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=418, Percent_Identity=43.7799043062201, Blast_Score=333, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=48, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=48, Blast_Score=358, Evalue=5e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_HYPNA (Q0C0I5)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760758.1
- ProteinModelPortal:   Q0C0I5
- SMR:   Q0C0I5
- STRING:   Q0C0I5
- GeneID:   4287323
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2060
- NMPDR:   fig|228405.5.peg.1985
- TIGR:   HNE_2060
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   IDMEQVA
- PhylomeDB:   Q0C0I5
- ProtClustDB:   CLSK2531974
- BioCyc:   HNEP81032:HNE_2060-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46422; Mature: 46291

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSAHVPETLAGGFFSVGLAERDPELAAAINQEATRQQHQIELIASENIVSRAVLEAQG
CCCCCCCCHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCC
SILTNKYAEGYPGKRYYGGCEFVDIAEELAIERAKKLFNCGFANVQPNSGSQANQGVFQA
CHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
VLKPGDTILGMSLAAGGHLTHGAKPNQSGKWFNAVQYGVRPEDHLIDFDEVERLARAHRP
HHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHCCCHHHHHHHHHHCCC
QMIIAGGSAYPRQIDFKRFREIADDVGAIFLVDMAHFAGLVAGGAHPNPLDHCHIATTTT
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
HKTLRGPRGGMILTNDEALAKKINSAIFPGIQGGPLMHVIAGKAVAFGEALMPEFKTYVE
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHHHHHHHHHHH
QVVSNARAMAAACRTAGLDVVSDGTDTHLALIDLRPKGVTGRDAEAALERAYITCNKNGI
HHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCCCCCCHHHHHHHEEEEECCCCC
PFDPAPPTVTSGIRVGSPAGTTRGFREEEFIQIGTWIGEIVDALANGNSDAVEARVREEV
CCCCCCCCHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KALTARFPIYEGLGG
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADSAHVPETLAGGFFSVGLAERDPELAAAINQEATRQQHQIELIASENIVSRAVLEAQG
CCCCCCCHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCC
SILTNKYAEGYPGKRYYGGCEFVDIAEELAIERAKKLFNCGFANVQPNSGSQANQGVFQA
CHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
VLKPGDTILGMSLAAGGHLTHGAKPNQSGKWFNAVQYGVRPEDHLIDFDEVERLARAHRP
HHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHCCCHHHHHHHHHHCCC
QMIIAGGSAYPRQIDFKRFREIADDVGAIFLVDMAHFAGLVAGGAHPNPLDHCHIATTTT
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
HKTLRGPRGGMILTNDEALAKKINSAIFPGIQGGPLMHVIAGKAVAFGEALMPEFKTYVE
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHHHHHHHHHHH
QVVSNARAMAAACRTAGLDVVSDGTDTHLALIDLRPKGVTGRDAEAALERAYITCNKNGI
HHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCCCCCCHHHHHHHEEEEECCCCC
PFDPAPPTVTSGIRVGSPAGTTRGFREEEFIQIGTWIGEIVDALANGNSDAVEARVREEV
CCCCCCCCHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KALTARFPIYEGLGG
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA